• 제목/요약/키워드: Bio-analysis

검색결과 3,812건 처리시간 0.035초

한우의 연령에 따른 혈액 및 혈청 화학치 분석 (Hematological and biochemical analysis of Korean indigenous cattle according to the ages)

  • 조현웅;고원석;손향원;이미진;송희종;박진호
    • 한국동물위생학회지
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    • 제31권1호
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    • pp.137-147
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    • 2008
  • Investigations for hematologic and biochemical values and the differential count of WBC for Korean indigenous cattle (KIC)is rarely performed. Therefore, when the index of complete blood counts (CBC) and biochemical analysis of KIC was requested, it had many difficulties to make the results for blood condition since the standard hematologic and biochemical values of KIC are lacking. In the present study, we have investigated the hematologic and biochemical values and the differential count of WBC for blood of total 252 KIC and 17 striped cattle (SC) as a control. As a result, the mean values of RBC and platelet of KIC were significantly decreased by age (P<0.05). The mean values of RBC, HCT, MCV and MCHC between KIC and SC of the same age (2 - 3 years) showed the statistical significance (P<0.05). Also, in the WBC of KIC, the mean values were decreased according to the age from $12.8{\times}10^3/{\mu}l$ under 1 year to $8.5{\times}10^3/{\mu}l$ over 5 years. In the differential count of WBC of KIC, it showed generally the rates of 45% lymphocyte and 35% segmented neutrophil. In the biochemical analysis, the mean values of BUN and globulin were significantly increased according to the age (P<0.05). Additionally, in comparative analysis between pregnant and non-pregnant group of KIC, the mean values of Hg and HCT in pregnant group were significantly decreased (P<0.05). In conclusion, data obtained from this study may be valuable as a standard for interpretation of the results in hematologic and biochemical analysis of KIC.

Development of a Simultaneous Analytical Method for Diquat, Paraquat and Chlormequat in Animal Products Using UPLC-MS/MS

  • Cho, Il Kyu;Rahman, Md. Musfiqur;Seol, Jae Ung;Noh, Hyun Ho;Jo, Hyeong-Wook;Moon, Joon-Kwan
    • 한국환경농학회지
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    • 제39권4호
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    • pp.368-374
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    • 2020
  • BACKGROUND: The residual analysis of polar pesticides has remained a challenge. It is even more difficult to simultaneously analyze multiple polar pesticides. Diquat, paraquat, and chlormequat are typical examples of highly polar pesticides. The existing methods for the analysis of diquat, paraquat and chlormequat are complex and time consuming. Therefore, a simple, quick and effective method was developed in the represent study for simultaneous analysis of diquat, paraquat and chlormequat in animal products, meat and fat using UPLC-MS/MS. METHODS AND RESULTS: Sample extraction was carried out using acidified acetonitrile and water and re- extracted with acidified acetonitrile and combine the extracts followed by centrifugation. The extract was then cleaned up with a HLB cartridge after reconstitution with acidic acetonitrile and water. The method was validated in quintuplicate at three different concentrations. The limits of detection (LOD) and quantification (LOQ) were 0.0015 and 0.005 mg/L, respectively. Matrix suppression effect was observed for all of the analytes. A seven point matrix matched calibration curve was constructed for each of the compound resulted excellent linearity with determination coefficients (R2) ≥ 0.991. Accuracy and precision of the method was calculated from the recovery and repeatability and ranged from 62.4 to 119.7% with relative standard deviation less than 18.8%. CONCLUSION: The recovery and repeatability of the developed method were in the acceptable range according to the Codex Alimentarius guideline. The developed method can be applied for the routine monitoring of diquat, paraquat, and chlormequat in animal products, meat and fat.

Agro-Healing Service Quality Analysis Using IPA Analysis for Business Owners

  • Kim, Yun-Jin;Kim, Seon-Ok;Park, Sin-Ae
    • 인간식물환경학회지
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    • 제24권6호
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    • pp.673-684
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    • 2021
  • Background and objective: This study seeks to investigate domestic agro-healing farm resources so that preliminary data can be obtained on the systematic provisions for agro-healing programs in order to improve service quality and to identify the quality of agro-healing policy services. Methods: For this study, owners of agro-healing facilities were asked 24 questions via an online survey. An importance-performance analysis (IPA) was conducted to identify service quality for each policy sector. Results: Respondents' satisfaction levels were low when compared to agro-healing farm owners' perceptions of the above-average importance of each sector. The results from the resource analysis of domestic agro-healing farms indicated that the main operational purposes of farms were, in descending order, experience (90.0%), healing (86.7%), and education (84.0%). With respect to the type of program that was operated, cultivating crops (horticultural therapy) was identified most frequently at 83.3%. Conclusion: As a result of this study, the resources possessed by agro-healing farms were identified. In addition, the results of the IPA analysis in terms of service quality are not as good as expected, and performance levels should be increased to improve this. The results of this study are expected to provide useful information not only to improve the quality of agro-healing services, but also to revitalize the agro-healing industry while developing a systematic agro-healing program.

광전 방식에 의한 피하 지방층의 비만도 측정에 관한 연구 (A Study on The Fat Measurement at Subcutaneous Adipose by Optical and Electrical Method)

  • 오세용;이영우
    • 한국정보통신학회:학술대회논문집
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    • 한국해양정보통신학회 2008년도 춘계종합학술대회 A
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    • pp.405-407
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    • 2008
  • 광학적 방식에 의한 비침습적으로 신체 전체의 비만도를 측정하기 위해서는 단일 특정 부위에서 측정하는 것은 오차가 너무 크기 때문에 여러 부위에서 측정하고 이를 통계적 방법에 의해서 정확도를 높일 수 있다. 이를 위해서 백색광 LED광원을 이용하고 전기적 방식인 BIA(Bio-electrical Impedance)방식으로 동시에 측정하여 광전기적 지방측정에 관한 상관식을 유도하였다.

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Post and core build-ups in crown and bridge abutments: Bio-mechanical advantages and disadvantages

  • Mamoun, John
    • The Journal of Advanced Prosthodontics
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    • 제9권3호
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    • pp.232-237
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    • 2017
  • Dentists often place post and core buildups on endodontically treated abutments for crown and bridge restorations. This article analyzes the bio-mechanical purposes, advantages and disadvantages of placing a core or a post and core in an endodontically treated tooth and reviews literature on post and core biomechanics. The author assesses the scientific rationale of the claim that the main purpose of a post is to retain a core, or the claim that posts weaken teeth. More likely, the main function of a post is to help prevent the abutment, on which a crown is cemented, from fracturing such that the abutment separates from the tooth root, at a fracture plane that is located approximately and theoretically at the level of the crown (or ferrule) margin. A post essentially improves the ferrule effect that is provided by the partial fixed denture prosthesis. This paper also explores the difference between bio-mechanical failures of crowns caused by lack of retention or excess taper, versus failures due to a sub-optimal ferrule effect in crown and bridge prostheses.

발효조건에 따른 돼지감자의 발효시스템 (Characteristics of Sunroot (Helianthus tuberosus) Fermentation with boundary condition)

  • 정성원;우지희;최원식
    • 한국산업융합학회 논문집
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    • 제20권5호
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    • pp.411-417
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    • 2017
  • In this study, Sunroot was used as material in the fermentation process. Sunroot are widely distributed in Korea, The main component is composed of inulin. Fermented vinegar stimulates digestion in the body, as well as to relieve stress is a cause of increasing the neurotransmitter-Serotonin of the body. Therefore, we conducted fermentation studies using sunroot. This research conducted seven days, and the pH and brix value analysis was done every 12 hours during fermentation process. The experimental results show that the fermentation product under blender treatment is better than cutting treatment and without cutting treatment. Initial brix 25% is the best treatment for fermentation because produce fermentation product with a good taste and aroma than other treatment.

범죄의 두려움에 대한 자극의 유형에 따른 ERP 변화 분석 (An Analysis on the Changes in ERP According to Type of Stimuli about Fear of Crime)

  • 김용우;강행봉
    • 한국멀티미디어학회논문지
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    • 제20권12호
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    • pp.1856-1864
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    • 2017
  • The ultimate goal of multimedia in bio-signal research is to approach multimedia contents through bio-signal. Hence it is important to interpret user's emotions by analyzing his or her bio-signals. In this paper, we construct ERP task of oddball component to analyze EEG signal between normal stimuli and fear stimuli and measure EEG during ERP task. The results from extracted ERP component show that there is a difference in N200 in visual stimuli, P300 in auditory stimuli, and N100 and P300. Moreover, there are larger changes in audiovisual stimuli, indicating that users recognize greater fear of crime when visual and auditory stimuli are simultaneously presented.

Gene Cloning, High-Level Expression, and Characterization of an Alkaline and Thermostable Lipase from Trichosporon coremiiforme V3

  • Wang, Jian-Rong;Li, Yang-Yuan;Liu, Danni
    • Journal of Microbiology and Biotechnology
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    • 제25권6호
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    • pp.845-855
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    • 2015
  • The present study describes the gene cloning and high-level expression of an alkaline and thermostable lipase gene from Trichosporon coremiiforme V3. Nucleotide analysis revealed that this lipase gene has an open reading frame of 1,692 bp without any introns, encoding a protein of 563 amino acid residues. The lipase gene without its signal sequence was cloned into plasmid pPICZαA and overexpressed in Pichia pastoris X33. The maximum lipase activity of recombinant lipase was 5,000 U/ml, which was obtained in fed-batch cultivation after 168 h induction with methanol in a 50 L bioreactor. The purified lipase showed high temperature tolerance, and being stable at 60℃ and kept 45% enzyme activity after 1 h incubation at 70℃. The stability, effects of metal ions and other reagents were also determined. The chain length specificity of the recombinant lipase showed high activity toward triolein (C18:1) and tripalmitin (C16:0).

첨단 유동가시화 기술을 이용한 수치해석 검증용 실험 (EXPERIMENTS FOR VALIDATING NUMERICAL ANALYSIS USING ADVANCED FLOW VISUALIZATION TECHNOLOGIES)

  • 이상준
    • 한국전산유체공학회:학술대회논문집
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    • 한국전산유체공학회 2008년도 학술대회
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    • pp.14-17
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    • 2008
  • Recently, several advanced flow visualization techniques such as Particle Image Velocimetry (PIV) including stereo PIV, holographic PIV, and dynamic PIV have been developed. These advanced techniques have strong potential as the experimental technology which can be used for verifying numerical simulation. In addition, there would be indispensable in solving complicated thermo-fluid flow problems not only in the industrial fields such as automotive, space, electronics, aero- and hydro-dynamics, steel, and information engineering, but also in the basic research fields of medical science, bio-medical engineering, environmental and energy engineering etc. Especially, NT Nano Technology) and BT (Bio Technology) strongly demand these advanced measurement techniques, because it is difficult for conventional methods to observe most complicated nano- and bio-fluidic phenomena. In this paper, the basic principle of these advanced visualization techniques and their practical applications which cannot be resolved by conventional methods, such as flow in automotive HVAC system, ship and propeller wake, three-dimensional flow measurement in micro-conduits, and flow around a circulating cylinder will be introduced.

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첨단 유동가시화 기술을 이용한 수치해석 검증용 실험 (EXPERIMENTS FOR VALIDATING NUMERICAL ANALYSIS USING ADVANCED FLOW VISUALIZATION TECHNOLOGIES)

  • 이상준
    • 한국전산유체공학회:학술대회논문집
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    • 한국전산유체공학회 2008년 추계학술대회논문집
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    • pp.14-17
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    • 2008
  • Recently, several advanced flow visualization techniques such as Particle Image Velocimetry (PIV) including stereo PIV, holographic PIV, and dynamic PIV have been developed. These advanced techniques have strong potential as the experimental technology which can be used for verifying numerical simulation. In addition, there would be indispensable in solving complicated thermo-fluid flow problems not only in the industrial fields such as automotive, space, electronics, aero- and hydro-dynamics, steel, and information engineering, but also in the basic research fields of medical science, bio-medical engineering, environmental and energy engineering etc. Especially, NT (Nano Technology) and BT (Bio Technology) strongly demand these advanced measurement techniques, because it is difficult for conventional methods to observe most complicated nano- and bio-fluidic phenomena. In this paper, the basic principle of these advanced visualization techniques and their practical applications which cannot be resolved by conventional methods, such as flow in automotive HVAC system, ship and propeller wake, three-dimensional flow measurement in micro-conduits, and flow around a circulating cylinder will be introduced.

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