• Title/Summary/Keyword: Bio-analysis

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Higher Extraction of Phytochemical Compounds from Tartary Buckwheat Seeds by the Application of Surfactant Formulation

  • Park, Cheol Ho;Azad, Md Obyedul Kalam;Heo, Jeong Won;Kang, Wie Soo
    • Korean Journal of Plant Resources
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    • v.30 no.6
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    • pp.686-692
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    • 2017
  • The aim of this study was to determine the suitability of surfactant to extract higher phenolic compound, flavonoid and antioxidant activity from Tartary buckwheat and evaluate the potentiality of surfactant as a screening agent for breeding purpose. Primarily, we employed two types of surfactant (Hydrophilic: Tween 20 and Lipophilic: Span 80) to select the suitable surfactant agent for the extraction of optimum bioactive compounds. Between two surfactants, Tween 20 showed highest efficiency at 4 mM concentration to extract total phenolic content (TP), total flavonoid (TF) and antioxidant activity (AA). Tween 20 at 4 mM concentration was fixed for further analysis along with hot water ($90^{\circ}C$) treatment as a control. In our findings, highest TP (118 mg/g), TF (38 mg/g) and AA (76%) was achieved in KW21 and KW22 among the fifteen accessions of Tartary buckwheat. In other way, TP, TF and AA was 200%, 120% and 110% higher in surfactant formulation compared with control treatment, respectively.

Differential expression of soybean SLTI100 gene encoding translation elongation factor 1A by abiotic stresses

  • Chung, Eun-Sook;Cho, Chang-Woo;So, Hyun-A;Yun, Bo-Hyun;Lee, Jai-Heon
    • Journal of Plant Biotechnology
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    • v.36 no.3
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    • pp.255-260
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    • 2009
  • The translation elongation factor 1A, eEF1A, catalyzes the binding of aminoacyl-tRNA to the A-site of the ribosome by a GTP-dependent mechanism. By subtractive suppression hybridization technique, we have isolated a soybean low-temperature inducible gene, SLTI100 encoding translation elongation factor 1A. Multiple sequence alignments and phylogenic analysis showed that SLTI100 and other eEF1As originated from diverse organisms are highly conserved. RNA expression of SLTI100 was specifically induced by low temperature, high salt, ABA, or drought stress. Based on the subcellular localization of the corresponding gene product fused to GFP, we were able to confirm that SLTI100-GFP was restricted to the nucleus and cytoplasm. We propose that soybean eEF1A may play an important role in translational regulation during abiotic stress responses in plants.

Computational Fluid Dynamics Analysis of the Pretreatment System for Livestock BIO-GAS MGT Power Generation (바이오가스 마이크로 터빈 발전용 전처리시스템 전산유동해석)

  • Hur, Kwang-Beom;Park, Jung-Keuk;Rhim, Sang-Gyu;Kim, Jae-Hoon
    • 한국전산유체공학회:학술대회논문집
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    • 2008.03b
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    • pp.260-263
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    • 2008
  • KEPCO(Korea Electric Power Corporation) is performing the nation's first biogas-MGT project as an effort to encourage the utilization of wasted biogas which contains useful CH4. The goals of this project are to develop the Pretreatment system of Livestock bio-gas and set up the biogas-MGT co-generation system. The project will not only utilze flared biogas as precious energy but also improve the economics of the plant a lot. The pretreatment system mainly consists of sulfur removal tower, biogas compressor and many filtering systems. A computational fluid dynamics study in the bio gas sulfur removal tower and sulfur absorption filter was carried out. Understanding of the flow in the sulfur removal tower and sulfur adsorption filter obtained by this study can be used to identify the problems in the sulfur removal tower and to improve the sulfur removal efficiency of the sulfur removal tower. Resistance material modeling is used to simulate the sulfur adsorption filter, and the resistance coefficient was adjusted to reflect the experimental pressure loss value. And the pressure loss change with the flowrate is predicted

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Development of u-Healthcare Agent System using of 3-Axis Accelerometer Sensor (3축 가속도 센서를 이용한 u-헬스케어 에이전트 시스템 개발)

  • Choi, Dong-Oun;Kim, Jin-Sung;Song, Haeng-Sook
    • The Journal of the Korea Contents Association
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    • v.10 no.4
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    • pp.98-105
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    • 2010
  • Health of the people involved in blood sugar, blood pressure, activity, and using bio-sensors to obtain biometric information, the system for monitoring health research for development is a lot. This paper analyzes the bio information by monitoring the u-health care agent system was developed. Activity measurement technology that uses 3-axis accelerometer sensor. 3-axis acceleration sensors, using information obtained from activity to activity analysis to calculate the exact algorithm was developed. More accurate than the existing system to calculate the calorie consumption, it is stored in the database management. and you can check your health status using of activity and bio-information.

Immunostimulant and Anti-Tumor Activity of Crude Extracts of Galium aparine L. (팔선초 물 추출물의 면역자극 및 항종양 활성)

  • Yoon, Taek-Joon;Lee, Chang-Kwon;Park, Tae-Kyu;Lee, Kwang-Ho
    • Korean Journal of Pharmacognosy
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    • v.36 no.4 s.143
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    • pp.332-337
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    • 2005
  • We here demonstrate the evidence of increased anti-tumor and immunostimulating activities of crude extracts (GAL) from Galium. aparine L. In experimental lung metastasis of colon26-M3.1 carcinoma or B16-BL6 melanoma cells, prophylactically intravenous (i.v) administration of GAL significantly inhibited lung metastasis in a dose-dependant manner. In an in vitro cytotoxicity analysis, GAL at the concentration up to $500\;{\mu}g/ml$ did not affect the growth of B16-BL6 melanoma cells. In contrast, GAL showed the enhancement of splenocyte proliferating activity in a dose-dependent manner. Peritoneal macrophages stimulated with GAL produced various cytokines such as $1L-1{\beta},\;TNF-{\alpha},\;IFN-{\gamma}$ and IL-12. These data suggest that GAL has an antitumor activity to inhibit tumor metastasis, and its antitumor effects is associated with activation of nonspecific immnune related cells.

Structure Prediction of the Peptide Synthesized with the Nonribosomal Peptide Synthetase Gene from Bradyrhizobium japonicum

  • JUNG BO-RA;LEE YUKYUNG;LIM YOONGHO;AHN JOONG-HOON
    • Journal of Microbiology and Biotechnology
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    • v.15 no.3
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    • pp.656-659
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    • 2005
  • Small peptides synthesized by nonribosomal peptide synthetases (NRPSs) genes are found in bacteria and fungi. While some microbial taxa have few, others make a large number and variety. However, biochemical characterization of the products synthesized by NPRS demands a great deal of efforts. Since the completion of genome projects of numerous microorganisms, the numbers of available NRPSs genes are being expanded. Prediction of the peptides encoded by NRPS could save time and efforts. We chose the NRPS gene from Bradyrhizobium japonicum as a model to predict the peptide structure encoded by NRPS genes. Using computational analyses, the domain structure of this gene was defined, and the structure of a peptide synthesized by this NRPS was deduced. It was found that it encoded a tripeptide consisting of proline-serine-phenylalanine. This method would be helpful to predict the structure of small peptides with various NPRS genes from the genome sequence.

Preparation of Micro-/Macroporous Carbons and Their Gas Sorption Properties

  • Hwang, Yong-Kyung;Shin, Hye-Seon;Hong, Jin-Yeon;Huh, Seong
    • Bulletin of the Korean Chemical Society
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    • v.35 no.2
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    • pp.377-382
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    • 2014
  • Micro-/macroporous carbons (MMCs) were prepared using a hollow mesoporous silica capsule (HMSC) as a sacrificial hard template. The carbonization process after the infiltration of furfuryl alcohol into the template-free HMSC material afforded MMC materials in high yield. The hard template HMSC could be removed by HF etching without deteriorating the structure of MMC. The MMC materials were fully characterized by SEM, TEM, PXRD, XPS, and Raman spectroscopy. The replication processes were so successful that MMCs exhibited a hollow capsular structure with multimodal microporosity. Detailed textural properties of MMC materials were investigated by volumetric $N_2$ adsorption-desorption analysis at 77 K. To explore the gas sorption abilities of MMCs for other gases, $H_2$ and $CO_2$ sorption analyses were also performed at various temperatures. The multimodal MMC materials were found to be good sorbents for both $H_2$ and $CO_2$ at low pressure.

Numerical Simulation for Urban Climate Assessment and Hazard (도시기후 평가와 방재를 위한 도시기상 수치모의)

  • O, Seong-Nam
    • Magazine of the Korean Society of Hazard Mitigation
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    • v.2 no.4 s.7
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    • pp.40-47
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    • 2002
  • Since it is important to understand the bio-climatic change in Seoul for ecological city planning in the future, this paper gives an overview on bio-climate analysis of urban environments at Seoul. We analyzed its characteristics in recent years using the observations of 24 of Automatic Weather Station (AWS) by Korea Meteorological Administration (KMA). In urbanization, Seoul metropolitan area is densely populated and is concentrated with high buildings. This urban activity changes land covering, which modifies the local circulation of radiation, heat and moisture, precipitation and creating a specific climate. Urban climate is evidently manifested in the phenomena of the increase of the air temperature, called urban heat Island and in addition urban sqall line of heavy rain. Since a city has its different land cover and street structure, these form their own climate character such as climate comfort zone. The thermal fold in urban area such as the heat island is produced by the change of land use and the air pollution that provide the bio-climate change of urban eco-system. The urban wind flow is the most important climate element on dispersion of air pollution, thermal effects and heavy shower. Numerical modeling indicates that the bio-climatic transition of wind wake in urban area and the dispersion of the air pollution by the simulations of the wind variation depend on the urban land cover change. The winds are separately simulated on small and micro-scale at Seoul with two kinds of kinetic model, Witrak and MUKLIMO.

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Combining Neuroinformatics Databases for Multi-Level Analysis of Brain Disorders

  • Yu, Ha Sun;Bang, Joon;Jo, Yousang;Lee, Doheon
    • Interdisciplinary Bio Central
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    • v.4 no.3
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    • pp.7.1-7.8
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    • 2012
  • With the development of many methods of studying the brain, the field of neuroscience has generated large amounts of information obtained from various techniques: imaging techniques, electrophysiological techniques, techniques for analyzing brain connectivity, techniques for getting molecular information of the brain, etc. A plenty of neuroinformatics databases have been made for storing and sharing this useful information and those databases can be publicly accessed by researchers as needed. However, since there are too many neuroinformatics databases, it is difficult to find the appropriate database depending on the needs of researcher. Moreover, many researchers in neuroscience fields are unfamiliar with using neuroinformatics databases for their studies because data is too diverse for neuroscientists to handle this and there is little precedent for using neuroinformatics databases for their research. Therefore, in this article, we review databases in the field of neuroscience according to both their methods for obtaining data and their objectives to help researchers to use databases properly. We also introduce major neuroinformatics databases for each type of information. In addition, to show examples of novel uses of neuroinformatics databases, we represent several studies that combine neuroinformatics databases of different information types and discover new findings. Finally, we conclude our paper with the discussion of potential applications of neuroinformatics databases.

Assessment of Driver's Emotional Stability by Using Bio-signals (생체신호 측정을 통한 운전자의 감정적 안정상태 평가)

  • Kim, Jung-Yong;Park, Ji-Soo;Yoon, Sang-Young
    • Journal of the Ergonomics Society of Korea
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    • v.30 no.1
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    • pp.203-211
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    • 2011
  • Objective: The aim of this study is to introduce a methodology to assess driver's emotion stability by using bio-signals. Background: Psychophysiological analysis of driver's behavior has been conducted to improve the driving safety and comfort. However, the variability of bio-signal and individual difference made it difficult to assess the psychophysiological status of drivers that can be expressed as emotional stability of drivers. Method: Two experimental studies were reviewed and summarized. New techniques assessing emotional stability of drivers were explained. Statistical concept and multidimensional space were used to identify the emotionally stable conditions. Conclusion: Psychophysiological approach can provide information of driver's emotional status. The experimental methodology and algorithm used in this study showed the possibility of parameterization of psychophysiological response. Application: Currently measured statistical and geometrical data can be further applied to develop an interactive device monitoring and reacting driver's emotion when driver experiences emotionally unstable or uncomfortable situation.