Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Differential expression of soybean SLTI100 gene encoding translation elongation factor 1A by abiotic stresses

  • Chung, Eun-Sook (BK21 Center for Silver-Bio Industrialization, College of Natural Resources and Life Science, Dong-A University) ;
  • Cho, Chang-Woo (BK21 Center for Silver-Bio Industrialization, College of Natural Resources and Life Science, Dong-A University) ;
  • So, Hyun-A (BK21 Center for Silver-Bio Industrialization, College of Natural Resources and Life Science, Dong-A University) ;
  • Yun, Bo-Hyun (Plant Biotechnology Department, College of Natural Resources and Life Science, Korea University) ;
  • Lee, Jai-Heon (BK21 Center for Silver-Bio Industrialization, College of Natural Resources and Life Science, Dong-A University)
  • Published : 2009.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

The translation elongation factor 1A, eEF1A, catalyzes the binding of aminoacyl-tRNA to the A-site of the ribosome by a GTP-dependent mechanism. By subtractive suppression hybridization technique, we have isolated a soybean low-temperature inducible gene, SLTI100 encoding translation elongation factor 1A. Multiple sequence alignments and phylogenic analysis showed that SLTI100 and other eEF1As originated from diverse organisms are highly conserved. RNA expression of SLTI100 was specifically induced by low temperature, high salt, ABA, or drought stress. Based on the subcellular localization of the corresponding gene product fused to GFP, we were able to confirm that SLTI100-GFP was restricted to the nucleus and cytoplasm. We propose that soybean eEF1A may play an important role in translational regulation during abiotic stress responses in plants.

Keywords

References

  1. Aguilar F, Montandon P-E, Stutz E (1991) Two genes encoding the soybean translation elongation factor eEF-1$\alpha$ are transcribed in seedlings leaves. Plant Mol Biol 17:351-360 https://doi.org/10.1007/BF00040630
  2. Axelos M, Bardet C, Liboz T, LeVan TA, Curie C, Lescure B (1989) The gene family encoding the Arabidopsis thaliana translation elongation factor EF-1$\alpha$: molecular cloning, characterization and expression. Mol Gen Genet 219:106-112 https://doi.org/10.1007/BF00261164
  3. Berberich T, Sugawara K, Harada M, Kusano T (1995) Molecular cloning, characterization and expression of an elongation factor 1$\alpha$ gene in maize. Plant Mol Biol 29:611-615 https://doi.org/10.1007/BF00020988
  4. Browing KS, Humphreys J, Hobbs W, Smith GB, Ravel JM (1990) Quantitation of translation initiation and elongation factors in wheat germ. J Biol Chem 265:17967-17973
  5. Carneiro N P, Hughes PA, Larkins BA (1999) The eEF1A gene family is differentially expressed in maize endosperm. Plant Mol Biol 41:801-813 https://doi.org/10.1023/A:1006391207980
  6. Cho C-W, Lee H-J, Chung E, Kim KM, Heo J E, Kim J-I, Chung J, Ma Y, Fukui K, Lee D-W, Kim D-H, Chung Y-S, Lee J-H (2007) Molecular characterization of the soybean L-asparaginase gene induced by low temperature stress. Mol Cells 23:280-286
  7. Church GM, Gilbert W (1984) Genomic sequencing. Proc Natl Acad Sci USA 81:1991-1995 https://doi.org/10.1073/pnas.81.7.1991
  8. Clore AM, Dannenhoffer JM, Larkins BA (1996) EF-1 alpha is associated with a cytoskeletal network surrounding protein bodies in maize endosperm cells. Plant Cell 8:2003-2014 https://doi.org/10.1105/tpc.8.11.2003
  9. Condeelis J (1995) Elongation factor 1 alpha, translation and the cytoskeleton. Trends Biochem Sci 20:169-170 https://doi.org/10.1016/S0968-0004(00)88998-7
  10. Davis SJ, Vierstra RD (1996) Soluble derivatives of green fluorescent protein (GFP) for use in Arabidopsis thaliana. Weeds World 3:43-48
  11. Dunn MA, Morris A, Jack PL, Hughes MA (1993) A low-temperature-responsive translation elongation factor 1$\alpha$ from barley (Hordeum vulgare L.). Plant Mol Biol 23:221-225 https://doi.org/10.1007/BF00021434
  12. Kidou S-I, Ejiri S-I (1998) Isolation, characterization and mRNA expression of four cDNAs encoding translation elongation factor 1A from rice (Oryza sativa L.). Plant Mol Biol 36: 137-148 https://doi.org/10.1023/A:1005960721762
  13. Kim K, Chung E, Cho CW, Soh HA, Chung YK, Seo YW, Lee SW, Lee YC, Chung YS, Lee JH (2009a) Molecular characterization of soybean ribosomal protein S13 targeted to the nucleus. Russ J Plant Physiol 56:131-138 https://doi.org/10.1134/S1021443709030145
  14. Kim K, Chung E, Cho CW, Soh HA, Yun BH, Kim JI, Seo YW, Lee SW, Lee YC, Chung YS, Lee JH (2009b) Cloning and characterization of SLTI98 encoding ribosomal protein S6 in soybean. Russ J Plant Physiol 56:118-125 https://doi.org/10.1134/S1021443709010178
  15. Lopez-Valenzuela JA, Gibbon BC, Holding DR, Larkins BA (2004) Cytoskeletal proteins are coordinately increased in maize genotypes with high levels of eEF1A. Plant Physiol 134:1784-1797 https://doi.org/10.1104/pp.103.032185
  16. Lopez-Valenzuela JA, Gibbon BC, Hughes PA, Dreher TW, Larkins BA (2003) eEF1A isoforms change in abundance and actin-binding activity during maize endosperm development. Plant Physiol 133:1285-1295 https://doi.org/10.1104/pp.103.027854
  17. Lund A, Knudsen SM, Vissing H, Clark B, Tommerups N (1996) Assignment of human elongation factor 1 genes: eEF1A maps to chromosome 6q14 and EF1A2 to 20q13.3. Genomics 36: 359-361 https://doi.org/10.1006/geno.1996.0475
  18. Metz AM, Timmer RT, Allen ML, Browning KS (1992) Sequence of a cDNA encoding the α-subunit of wheat translation elongation factor 1. Gene 120:315-316 https://doi.org/10.1016/0378-1119(92)90113-4
  19. Morelli J, Shewmaker CK, Vayada ME (1994) Biphasic stimulation of translational activity correlates with induction of translation elongation factor 1 subunit $\alpha$ upon wounding in potato tubers. Plant Physiol 106:897-903 https://doi.org/10.1104/pp.106.3.897
  20. Saitou N, Nei M (1987) The neighbor-joining method: A new method for constructing phylogenetic trees. Mol Biol Evol 4:406-425
  21. Thivierge K, Cotton S, Dufresne PJ, Mathieu I, Beauchemin C, Ide C, Fortin MG, Laliberte J-F (2008) Eukaryotic elongation factor 1A interacts with Turnip mosaic virus RNA-dependent RNA polymerase and VPg-Pro in virus-induced vesicles. Virol 377:216-225 https://doi.org/10.1016/j.virol.2008.04.015
  22. Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673-4680 https://doi.org/10.1093/nar/22.22.4673
  23. Vayada ME, Shewmaker CK, Morelli JK (1995) Translational arrest in hypoxic potato tubers is correlated with the aberrant association of elongation factor EF-1$\alpha$ with polysomes. Plant Mol Biol 28:751-757 https://doi.org/10.1007/BF00021198
  24. Vijaykumar D, Ramachander TVN, Mahishi LH, Kaul R, Pyati P, Paul B, Rawal SK (2002) Molecular cloning, characterization and tissue specific expression of an elongation factor 1A gene in Saccharum officinarum L. Plant Sci 162:315-321 https://doi.org/10.1016/S0168-9452(01)00579-9
  25. Xu W-L, Wang X-L, Wang H, Li X-B (2007) Molecular characterization and expression analysis of nine cotton GhEF1A genes encoding translation elongation factor 1A. Gene 389: 27-35 https://doi.org/10.1016/j.gene.2006.09.014

Cited by

  1. Gene coding for an elongation factor is involved in resistance against powdery mildew in common bean vol.130, pp.5, 2017, https://doi.org/10.1007/s00122-017-2864-x
  2. Heat-induced accumulation of protein synthesis elongation factor 1A implies an important role in heat tolerance in potato vol.244, pp.3, 2016, https://doi.org/10.1007/s00425-016-2534-2
  3. A Leader Intron of a Soybean Elongation Factor 1A (eEF1A) Gene Interacts with Proximal Promoter Elements to Regulate Gene Expression in Synthetic Promoters vol.11, pp.11, 2016, https://doi.org/10.1371/journal.pone.0166074
  4. Systemic responses in a tolerant olive (Olea europaea L.) cultivar upon root colonization by the vascular pathogen Verticillium dahliae vol.6, 2015, https://doi.org/10.3389/fmicb.2015.00928
  5. Roles of Protein Synthesis Elongation Factor EF-Tu in Heat Tolerance in Plants vol.2012, 2012, https://doi.org/10.1155/2012/835836