• Korean Journal of Food Science and Technology
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• v.49 no.1
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• pp.50-55
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• 2017

The purpose of this study was to investigate the effect of frying number on oxidative changes in edible oils and fried foods. According to the frying number, the extracted edible oils from pork cutlet and fried potato were used as experimental samples. The Ministry of Food and Drug Safety (MFDS) regulations permit edible oils to have <2.5 mg KOH/g of acid value and <50 meq/kg of peroxide value in food. However, there are no regulations for edible oils used to fry livestock. Animal foods contain protein and fat, and should be held to a different standard than ordinary food. Therefore, we present basic information and suggest the establishment of regulations for livestock frying oil and fried livestock.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.8
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• pp.246-253
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• 2018

The main industries in Chungcheongbuk-do are the smart IT parts; bio-health; and transportation machinery, materials and parts. The transportation, machinery, materials and parts (TMMP) industry is a key source technology for environmentally friendly and intelligent vehicles related to future vehicles in the machinery sector. Business support projects to improve this industry are currently underway. In this study, the number of enterprises, number of employees, amount of shipment, and added value of the enterprises in Chungbuk area were analyzed for the downstream and rear industries of the transportation, machinery, materials, and parts industry to set targets and directions for the technical support projects during the enterprise support. In particular, for the SMEs with less than 50 employees, the changes in the value added per enterprise were analyzed according to the period from the year of foundation. In the case of the rear industry, the value added by companies within three years after foundation has decreased gradually each year, so technical support is needed at the beginning of the startup. Companies between the ages of five and nine years are showing a fluctuating trend each year. Therefore, it is necessary to support business diversification or find new items.

• The Journal of the Korea Contents Association
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• v.12 no.4
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• pp.407-416
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• 2012

The primary purpose of this paper is to identify the development of education and training for the human resource development in the view of technology management. For this purpose, a research is developed based on the relevant literature reviews and empirical studies. Data have been collected from 110 firms in Daejeon city and tested by the frequency analysis and various statistical methods. The results of this empirical studies are summarized as follows. We classified in company competitive power as innovators, leading chasers and late chasers. The innovators, leading chasers and late chasers demanded the management of technology. They also demanded the middle manager, the CEO and R&D researchers as a important position in this demanding of the management of technology and its human resources of the market and technologies. Department of marketing and the executives and staff members find it important. We departmentalized the management of technology with the Daejeon region's strategic industries. Information technology(IT) industries thought that the middle manager, CEO and R&D department were important. Bio-industries thought that the CEO and the middle manager and R&D department were important. The high-tech parts materials industries thought that the middle manager, R&D department and marketing department considered as important.

• Microbiology and Biotechnology Letters
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• v.35 no.1
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• pp.1-10
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• 2007

The 44 endophytic bacterial strains were isolated from surface-sterilized root of rice cultivated in seven different locations of Chungcheong province, Korea. Each isolate was introduced into rice seedlings grown gnotobiotically by inoculating scissor-cut first true leaf with cell suspensions, and the colonization capacity of each isolate in root tissue was analyzed at 7 days after inoculation. Sixteen out of 44 isolates were re-isolated from root successfully with the frequency of $10^{3-5}$ CFU/g tissue. Interestingly, seven out of 16 isolates were identified as Burkholderia species. The identity between inoculated strains and re-isolates was confirmed by genomic finger-printing and 16S rDNA sequence analysis. By a confocal laser scanning microscopic observation it was revealed that KJ001 strain, one of the sixteen isolates tagged with gfp colonized in root tissue especially around xylem. Six out of seven Burkholderia strains obtained in this study showed antagonizing activities against seven different fungal pathogens, contain nifH gene, and five of them enhanced growth of cucumber over 30%. The isolates showed no hypersensitive response on tobacco leaves and no pathogenecity in rice. From these results it was found that the endophytic Burkholderia strains will be useful in agriculture to develop a biocontrol agent or a bio-fertilizer.

• Journal of Korean Society of Environmental Engineers
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• v.27 no.4
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• pp.353-361
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• 2005

In this study, we performed whole effluent toxicity(WET) test and compared the biological component analyses tests for some streams into which wastewater treatment plant effluents are discharged. The test procedures for the WET test and the biological component analysis were conducted according to U.S. EPA guidelines and the Ohio EPA wading method. The WET tests based on the test species of D. manga and S. capricornutum All treatment plants showed a S. capricornutum toxicity, and at 7 sites, except A and J, we could observe a D. magna toxicity. The highest toxicity was observed at site D and this were followed by E and F. The biological component analyses applied to the effluent discharged stream. We found that total species were low at sites C1, D1 and J1 and the low number of total individuals were observed at sites A1, C1, E1, H1, and J1 compared to the controls and other sites. The species richness was low at sites A1, B1, C1, D1, and J1. The species change was evident at site D(D1) and the tolerant species was predominant. The biological analyses and WET tests suggested that the effluents resulted in massive ecosystem impact.

• Journal of The Korean Society of Agricultural Engineers
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• v.59 no.4
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• pp.97-107
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• 2017

This study is to determine the coefficients of regression equations and to select the optimal regression equation in the LOADEST model after classifying the whole study period into 5 flow conditions for 16 watersheds located in the Nakdonggang waterbody. The optimized coefficients of regression equations were derived using the gradient descent method as a learning method in Tensorflow which is the engine of machine-learning method. In South Korea, the variability of streamflow is relatively high, and rainfall is concentrated in summer that can significantly affect the characteristic analysis of pollutant loads. Thus, unlike the previous application of the LOADEST model (adjusting whole study period), the study period was classified into 5 flow conditions to estimate the optimized coefficients and regression equations in the LOADEST model. As shown in the results, the equation #9 which has 7 coefficients related to flow and seasonal characteristics was selected for each flow condition in the study watersheds. When compared the simulated load (SS) to observed load, the simulation showed a similar pattern to the observation for the high flow condition due to the flow parameters related to precipitation directly. On the other hand, although the simulated load showed a similar pattern to observation in several watersheds, most of study watersheds showed large differences for the low flow conditions. This is because the pollutant load during low flow conditions might be significantly affected by baseflow or point-source pollutant load. Thus, based on the results of this study, it can be found that to estimate the continuous pollutant load properly the regression equations need to be determined with proper coefficients based on various flow conditions in watersheds. Furthermore, the machine-learning method can be useful to estimate the coefficients of regression equations in the LOADEST model.

• Journal of the Korean Applied Science and Technology
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• v.37 no.4
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• pp.830-838
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• 2020

The present study aimed to evaluate the effect of radiation mutant Perilla frutescens var. crispa on bone metabolism and the inflammatory response in a monosodium iodoacetateinduced rat model of osteoarthritis. radiation mutant Perilla frutescens var. crispa was administered orally at doses of 25, 50 or 100 mg/kg/day for 2 weeks before direct injection of monosodium iodoacetate (3 mg/50 μl of 0.9% saline) into the intra-articular space of the rats' right knees. The rats subsequently received the same doses of oral radiation mutant Perilla frutescens var. crispa for another 4 weeks. It was evaluated that the treatment effects based on serum biomarkers, and morphological and histopathological analysis of the knee joints. Compared with those in control rats, the radiation mutant Perilla frutescens var. crispa treatments significantly reduced the serum levels of inflammation, bone metabolism markers (i.e., COX-2, LTB4, MMP-3, and COMP), and the amount of fibrous tissue. Otherwise, it was significantly increased the concentration of TIMP-1 and calcitonin. In addition, the radiation mutant Perilla frutescens var. crispa treatments effectively preserved the knee cartilage and synovial membrane. As a result, it indicates that radiation mutant Perilla frutescens var. crispa prevented and alleviated osteoarthritis symptoms. Thus, radiation mutant Perilla frutescens var. crispa can be used in food and drug material for the management of osteoarthritis.

• The Korean Journal of Pesticide Science
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• v.20 no.3
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• pp.189-196
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• 2016

Several microorganisms in particular Bacillus subtilis group have been isolated from diverse places such as soils and the gastrointestinal tract of ruminants etc., and used as biocontrol agent against various plant pathogens and utilized as plant growth promoting agents. Among them, Bacillus is well known as one of the most useful bacteria for biocontrol and plant growth promotion. Bacterium GH1-13 was isolated from a reclaimed paddy field in Wando Island and identified as Bacillus velezensis using phylogenetic analysis on the basis of 16S rRNA and gyrB gene. It was confirmed that GH1-13 produced indole acetic acid (IAA) associated with promoted growth of rice root. GH1-13 showed characteristics of antagonization against the main pathogen of rice as well as diverse pathogenic fungi. GH1-13 had biosynthetic genes, bacillomycin, bacilycin, fengycin, iturin, and surfactin which are considered to be associated closely with inhibition of growth of pathogenic fungi and bacteria. This study showed that GH1-13 could be used as a multifunctional agent for biocontrol and growth promotion of crop.

• The Korean Journal of Malacology
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• v.32 no.2
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• pp.127-131
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• 2016

The natives of the genus Corbicula have shown worldwide dispersion in recent times, which has caused great ecological and economic impacts on the introduced ecosystems. The species reported from the genus have been consumed as food and explored for medicine with pharmacological activity. Consequently, the demand of Corbicula sp. in the South Korean domestic market has increased and so also it's associated import to the country. However, due to the absence of identification keys of imported Corbicula, the market is facing confronting situations. We hypothesized that the mitochondrial Cytochrome Oxidase I gene (CO-I) based molecular profiling could be a necessary technique for identification of Corbicula sp. in the South Korea domestic market. The genetic analysis identified both Corbicula japonica and Corbicula fluminea from the market foods. C. japonica and C. fluminea are inhabitants in Korea, but C. fluminea production has decreased in Seomjingang river basin. Therefore, C. fluminea identified from this study, is expected to be imported from China and would have a mixed sales in Seomjingang river side basin.

• Journal of Microbiology and Biotechnology
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• v.21 no.8
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• pp.808-817
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• 2011

Because of its elevated cellulolytic activity, the filamentous fungus Trichoderma harzianum has a considerable potential in biomass hydrolysis applications. Trichoderma harzianum cellobiohydrolase I (ThCBHI), an exoglucanase, is an important enzyme in the process of cellulose degradation. Here, we report an easy single-step ion-exchange chromatographic method for purification of ThCBHI and its initial biophysical and biochemical characterization. The ThCBHI produced by induction with microcrystalline cellulose under submerged fermentation was purified on DEAE-Sephadex A-50 media and its identity was confirmed by mass spectrometry. The ThCBHI biochemical characterization showed that the protein has a molecular mass of 66 kDa and pI of 5.23. As confirmed by smallangle X-ray scattering (SAXS), both full-length ThCBHI and its catalytic core domain (CCD) obtained by digestion with papain are monomeric in solution. Secondary structure analysis of ThCBHI by circular dichroism revealed ${\alpha}$- helices and ${\beta}$-strands contents in the 28% and 38% range, respectively. The intrinsic fluorescence emission maximum of 337 nm was accounted for as different degrees of exposure of ThCBHI tryptophan residues to water. Moreover, ThCBHI displayed maximum activity at pH 5.0 and temperature of $50^{\circ}C$ with specific activities against Avicel and p-nitrophenyl-${\beta}$-D-cellobioside of 1.25 U/mg and 1.53 U/mg, respectively.