The Korean Journal of Malacology (한국패류학회지)

The Malacological Society of Korea (한국패류학회)
권호 표지
DOI QR코드

DOI QR Code

A Mitochondiral Cytochrome Oxidase I gene based identification of Corbicula ssp. commercially available in South Korea

CO-I 유전자 기반 국내 유통 Corbicula 속 패류의 종 동정

  • Park, So Young (Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University) ;
  • Kang, Se Won (Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University) ;
  • Hwang, Hee Ju (Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University) ;
  • Chung, Jong Min (Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University) ;
  • Song, Dae Kwon (Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University) ;
  • Park, Hong Seog (Research Institute, GnC BIO Co., LTD.) ;
  • Han, Yeon Soo (Division of Plant Biotechnology, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Lee, Jun-Sang (Institute of Environmental Research, Kangwon National University) ;
  • Kang, Jung-Ha (National Institute of Fisheries Science, Biotechnology Research Division) ;
  • Lee, Yong Seok (Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University)
  • 박소영 (순천향대학교 자연과학대학 생명시스템학과) ;
  • 강세원 (순천향대학교 자연과학대학 생명시스템학과) ;
  • 황희주 (순천향대학교 자연과학대학 생명시스템학과) ;
  • 정종민 (순천향대학교 자연과학대학 생명시스템학과) ;
  • 송대권 (순천향대학교 자연과학대학 생명시스템학과) ;
  • 박홍석 ((주)지앤시바이오) ;
  • 한연수 (전남대학교 농업생명과학대학 식물생명공학부) ;
  • 이준상 (강원대학교 환경연구소) ;
  • 강정하 (국립수산과학원 생명공학과) ;
  • 이용석 (순천향대학교 자연과학대학 생명시스템학과)
  • Received : 2016.06.01
  • Accepted : 2016.06.30
  • Published : 2016.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

The natives of the genus Corbicula have shown worldwide dispersion in recent times, which has caused great ecological and economic impacts on the introduced ecosystems. The species reported from the genus have been consumed as food and explored for medicine with pharmacological activity. Consequently, the demand of Corbicula sp. in the South Korean domestic market has increased and so also it's associated import to the country. However, due to the absence of identification keys of imported Corbicula, the market is facing confronting situations. We hypothesized that the mitochondrial Cytochrome Oxidase I gene (CO-I) based molecular profiling could be a necessary technique for identification of Corbicula sp. in the South Korea domestic market. The genetic analysis identified both Corbicula japonica and Corbicula fluminea from the market foods. C. japonica and C. fluminea are inhabitants in Korea, but C. fluminea production has decreased in Seomjingang river basin. Therefore, C. fluminea identified from this study, is expected to be imported from China and would have a mixed sales in Seomjingang river side basin.

CO-I서열의 생물정보학적 분석 결과 국내에서 유통되는 재첩류의 연체부는 일본재첩 (C. japonica) 과 재첩 (C. fluminea) 으로 동정되어 두 종이 혼재하여 유통되는 것으로 확인되었다. 일본재첩 (C. japonica) 과 재첩 (C. fluminea) 은 국내에서 서식하고 있으나, 섬진강 유역의 재첩 (C. fluminea) 은 개체수가 감소하여 거의 생산되지 않고 있으므로, 본 연구를 통해 확인된 재첩 (C. fluminea) 은 중국 수입산으로 예상된다.

Keywords

References

  1. Altschul, S.F., Gish, W., Miller, W., Myers, E.W., and Lipman, D.J. (1990) Basic local alignment search tool. Journal of molecular biology, 215: 403-410. https://doi.org/10.1016/S0022-2836(05)80360-2
  2. Bang, I.S., and Lee, Y.S. (2014) Molecular Phylogenetic Study of Nesiohelix samarangae Based on CO-I Gene. The Korean Journal of Malacology, 30: 391-397. https://doi.org/10.9710/kjm.2014.30.4.391
  3. Chung, P.-., Park, G.-., Jung, Y., Yong, T.-., Im, K.-., and Soh, C.-. (2000) Mdicinal Mollusks in Korea. The Korean Journal of Malacology, 16: 55-60.
  4. Ewing, B., and Green, P. (1998) Base-calling of automated sequencer traces using phred. II. Error probabilities. Genome. Res., 8: 186-194. https://doi.org/10.1101/gr.8.3.186
  5. Ewing, B., Hillier, L., Wendl, M.C., and Green, P. (1998) Base-calling of automated sequencer traces using phred. I. Accuracy assessment. Genome. Res., 8: 175-185. https://doi.org/10.1101/gr.8.3.175
  6. Hebert, P.D.N., Ratnasingham, S., and de Waard, J.R. (2003) Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Proceedings of the Royal Society of London B: Biological Sciences, 270: S96-S99. https://doi.org/10.1098/rsbl.2003.0025
  7. Hee Ju, H., Se Won, K., So Young, P., Jong Min, C., Dae Kwon, S., Hyeongchun, P., Hong Seog, P., Yeon Soo, H., Jun-Sang, L., and Yong Seok, L. (2016) Classification and Phylogenetic Studies of Cephalopods from four countries of South-East Asia. The Korean Journal of Malacology, 32: 55-62. https://doi.org/10.9710/kjm.2016.32.1.55
  8. Higgins, D.G., and Sharp, P.M. (1988) CLUSTAL: a package for performing multiple sequence alignment on a microcomputer. Gene, 73: 237-244. https://doi.org/10.1016/0378-1119(88)90330-7
  9. Huang, X., and Madan, A. (1999) CAP3: A DNA sequence assembly program. Genome. Res., 9: 868-877. https://doi.org/10.1101/gr.9.9.868
  10. Kang, S.W., Hwang, H.J., Park, S.Y., Wang, T.H., Park, E.B., Lee, T.H., Hwang, U.W., Lee, J.-S., Park, H.S., Han, Y.S., Lim, C.E., Kim, S., and Lee, Y.S. (2014) Mollusks Sequence Database: Version II. The Korean Journal of Malacology, 30: 429-431. https://doi.org/10.9710/kjm.2014.30.4.429
  11. Kang, S.W., Park, S.Y., Patnaik, B.B., Hwang, H.J., Kim, C., Kim, S., Lee, J.S., Han, Y.S., and Lee, Y.S. (2015) Construction of PANM Database (Protostome DB) for rapid annotation of NGS data in Mollusks. The Korean Journal of Malacology, 31: 243-247. https://doi.org/10.9710/kjm.2015.31.3.243
  12. Kim, W.-K., Lee, C.-S., Lee, J.-Y., and Hu, S.-B. (2002) Production of Artificial Seedling of the Brackish Water Clam, Corbicula japonica. Journal of Aquaculture, 15: 23-29.
  13. Kress, W.J., Garcia-Robledo, C., Uriarte, M., and Erickson, D.L. (2015) DNA barcodes for ecology, evolution, and conservation. Trends in Ecology & Evolution, 30: 25-35. https://doi.org/10.1016/j.tree.2014.10.008
  14. Layton, K.K.S., Martel, A.L., and Hebert, P.D.N. (2014) Patterns of DNA Barcode Variation in Canadian Marine Molluscs. PLoS. ONE, 9: e95003. https://doi.org/10.1371/journal.pone.0095003
  15. Lee, J.-Y., Kim, W.-k., and Lee, C.-s. (2011) Growth and survival of the brackish water clam, Corbicula japonica larvae according to rearing conditions. The Korean Journal of Malacology, 27: 337-343. https://doi.org/10.9710/kjm.2011.27.4.337
  16. Lee, J.B., Shin, Y.J., Lee, J.H., Choi, Y.M., Lee, D.W., and Cha, H.K. (2012) Estimation of potential fishery yield for Corbicula japonica in the Seomjin River, Korea. The Korean Journal of Malacology, 28: 91-98. https://doi.org/10.9710/kjm.2012.28.2.091
  17. Lee, J.S. (2015) National list of species of the Korea [Invertebrates-VI] pp. National Institute of Biological Resources.
  18. Lee, Y.S., Jo, Y.-H., Kim, D.-S., Kim, D.-W., Kim, M.-Y., Choi, S.-H., Yon, J.-O., Byun, I.-S., Kang, B.-R., Jeong, K.-H., and Park, H.-S. (2004) Construction of BLAST Server for Mollusks. Korean Journal of Malacology, 20: 165-169.
  19. Musto, M. (2011) DNA quality and integrity of nuclear and mitochondrial sequences from beef meat as affected by different cooking methods. Food Technology and Biotechnology, 49: 523-528.
  20. Park, J.-K., and Kim, W. (2003) Two Corbicula (Corbiculidae: Bivalvia) mitochondrial lineages are widely distributed in Asian freshwater environment. Molecular Phylogenetics and Evolution, 29: 529-539. https://doi.org/10.1016/S1055-7903(03)00138-6
  21. Ryu, D.K., Chung, E.-Y., and Kim, Y.H. (2005) Age and Growth of the Brackish Water Clam, Corbicula japonica Prime on the West Coast of Korea. The Korean Journal of Malacology, 21: 57-64.
  22. Ministry of Food and Drug Safety (2015) Status of importation for fishery product
  23. Tamura, K., Stecher, G., Peterson, D., Filipski, A., and Kumar, S. (2013) MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Molecular Biology and Evolution, 30: 2725-2729. https://doi.org/10.1093/molbev/mst197

Cited by

  1. (Müller, 1774) Cultured in Çine Creek, Aydin, Turkey vol.37, pp.3, 2018, https://doi.org/10.2983/035.037.0304