• Journal of the Korean Applied Science and Technology
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• 제29권1호
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• pp.122-128
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• 2012

The influences of fluorescence, scattering, and flocculation in turbid material by light scattering of L-aspartic acid, preservative and emulsifier were interpreted for the scattered fluorescence intensity and wavelength. They have been studied the molecular properties by the spectroscopy of laser induced fluorescence (LIF) and flocculation. The effects of optical properties in scattering media have been found by the optical parameters(${\mu}_s$, ${\mu}_a$, ${\mu}_t$). Flocculation is an important step in many solid-liquid separation processes and is widely used. When two particles approach each other, interactions of several colloid particles can come into play which may have major effect on the flocculation and LIF process. The values of scattering coefficient ${\mu}_s$ are large by means of the increasing scattering of scatterer, The values have been found that the slope decays exponentially as a function of concentration from laser source to detector by our experimental result. It may also aid in designing the best model for oil chemistry, bio-pharmaceutical products, laser medicine and application of medical engineering on LIF and coagulation in particle transport mode.

• KSBB Journal
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• 제27권6호
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• pp.381-386
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• 2012

Obesity increases the risk of many adult diseases, such as atherosclerosis, diabetes, hypertension, ischemic heart disease and breast cancer. Inhibition of adipogenesis is an effective way in the anti-obesity management. Because of main components of Saururus chinensis is flavonoid, it has been showed some improvement by its antioxidant effects on the atherosclerosis, heart disease and diabetic hyperlipidemia. But mechanism of anti-obesity effect of S. chinensis is not clear. We investigated the effects of ethanol extracts of S. chinensis on adipogenesis in the 3T3-L1 pre-adipocyte. The 3T3-L1 cell line is commonly used to study adipogenesis in vitro. In this study, ethanol extracts of S. chinensis significantly decrease the lipid accumulation in the 3T3-L1 cells proved by measuring triglyceride contents and Oil red O staining. The proposed mechanism of inhibition of adipogenesis in the 3T3-L1 cells with ethanol extracts of S. chinensis is down-regulation of transcriptional factors and adipocyte-specific genes such CCAAT/enhancer binding protein ${\alpha}$ ($C/EBP{\alpha}$) and Peroxisome proliferator activated receptor ${\gamma}$ ($PPAR{\gamma}$) in concentration dependent pattern. These results suggest that ethanol extracts of S. chinensis inhibits adipognesis in the 3T3-L1 cells and can be used as a safe and efficient natural substance to manage anti-obesity.

• Journal of Korean Society of Environmental Engineers
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• 제35권6호
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• pp.389-393
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• 2013

The purpose of this study is to investigate the biodegradation characteristics of the xylene by BTEX-degrading bacteria, Pseudomonas putida BJ10, isolated from oil-contaminated soil and bio-degradation pathway of the xylene. The removal efficiencies of o, m, p-xylene in mineral salts medium (MSM) by P. putida BJ10 were 94, 90 and 98%, respectively for 24 hours. It shows clear difference compared with the control groups which were below 3%. The removal efficiencies of BTEX by P. putida BJ10 in gasoline-contaminated soil were 66% for 9 days. They were clearly distinguished from the control groups (control and sterilized soil) which were 32 and 8%. 3-methylcatechol and o-toluic acid were detected after 6 and 24 hours during the o-xylene biodegradation pathway. Therefore, we confirmed o-toluic acid as the final metabolite. And intermediate-products were somewhat different with previously published studies of the transformation pathway from o-xylene to 3-methylcatechol.

• Korean journal of food and cookery science
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• 제21권4호
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• pp.433-439
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• 2005

Soybean is an important plant as it is the source of protein and oil as well as various phytochemicals that are related with biological activity. Over the past decades, scientists have conducted considerable research on the physiological properties of soybeans, especially isoflavones, which are the characteristic components in soybeans. However, there is no research on the properties or the bio-functionality of soybean leaf. Jangachi and kimchi are two of the traditional special dishes of Gyungsang Province in Korea which we made from soybean leaves. Depending on the recipe, green or yellow soybean leaves are used for the preparation of these two side dishes. We compared the antioxidative activity and measured the contents of isoflavones, total phenol, chlorophylls, carotenoids, and vitamin C in the ingredients (green and yellow soybean leaf) and the final side dishes (jangachi and kimchi). We Int report that isoflavones were contained in soybean leaf and that jangachi had the highest isoflavone contents among the samples. Yellow soybean leaf contained higher isoflavones than green soybean lear and kimchi. From the TEAC assay results, the sequence or antioxidative activities was yellow soybean leaf > soybean leaf jangachi > green soybean leaf > soybean leaf kimchi. The sequence was the same with total phenol contents, indicating that antioxidative activity is highly related with total phenol level. Chlorophylls, carotenoids and vitamin C existed abundantly in green soybean leaf. In conclusion, soybean leaf could be a good material for health due to the presence of isoflavones and the other useful antioxidants mentioned above.

• Journal of the Korea Society of Computer and Information
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• 제21권8호
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• pp.57-64
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• 2016

Initial prevention activities and rapid propagation conditions is the most important to prevent diffusion of water pollution. If water pollutants flow into streams river or main stresm located in environmental conservation area or water intake facilities, we must predict immediately arrival time and the diffusion concentration to the proactive. National Institute of Environmental Research developed water pollution incident response prediction system linking dam and movable weir. the system is mathematical model which is updated daily. Therefore it can quickly predict the arrival time and the diffusion concentration when there are accident of oil spills and hazardous chemicals. Also we equipped with mathematical model and toxicity model of EFDC(Environmental Fluid Dynamics Code) to calculate the arrival time and the diffusion concentration. However these systems offer the services of an offline manner than real-time control services. we have ensured the reliability of data collection and have developed a real-time water quality measurement data transmission device by using the data linkage utilizing a mode bus communication and a commercial SCADA system, in particular, we implemented to be able to do real-time water quality prediction through information infrastructure of the water quality integrated management business created by utilizing the construction of the real-time prediction system that utilizes the data collected, the Open map, the visual representation using charts API and development of integrated management system development based on web maps.

• Korean Chemical Engineering Research
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• 제45권6호
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• pp.611-618
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• 2007

We demonstrate the sol-gel coating technique of colloidal clusters for producing hollow micro-particles with complex morphologies. Cross-linked amidine polystyrene (PS) microspheres were synthesized by emulsifier-free emulsion copolymerization of styrene and divinylbenzene. The amidine PS particles were self-organized inside toluene-in-water emulsion droplets to produce large quantities of colloidally stable clusters. These clusters were coated with thin silica shell by sol-gel reaction of tetraethylorthosilicate (TEOS) and ammonia, and the organic polystyrene cores were removed by calcination at high temperature to generate nonspherical hollow micro-particles with complex morphologies. This process can be used to prepare hollow particles with shapes such as doublets, tetrahedra, icosahedra, and others.

• The Plant Pathology Journal
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• 제27권1호
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• pp.59-67
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• 2011

A bacterial strain CH-67 which exhibits antagonism towards several plant pathogenic fungi such as Botrytis cinerea, Fulvia fulva, Rhizoctonia solani, Sclerotinia sclerotiorum, Colletotrichum sp. and Phytophthora sp. was isolated from forest soil by a chitin-baiting method. This strain was identified as Burkholderia cepacia complex (Bcc) and belonging to genomovar IX (Burkholderia pyrrocinia) by colony morphology, biochemical traits and molecular method like 16S rRNA and recA gene analysis. This strain was used to develop a bio-fungicide for the control of tomato leaf mold caused by Fulvia fulva. Various formulations of B. pyrrocinia CH-67 were prepared using fermentation cultures of the bacterium in rice oil medium. The result of pot experiments led to selection of the wettable powder formulation CH67-C containing modified starch as the best formulation for the control of tomato leaf mold. CH67-C, at 100-fold dilution, showed a control value of 85% against tomato leaf mold. Its disease control efficacy was not significantly different from that of the chemical fungicide triflumidazole. B. pyrrocinia CH-67 was also effective in controlling damping-off caused by Rhizoctonia solani PY-1 in crisphead lettuce and tomato plants. CH67-C formulation was recognized as a cell-free formulation since B. pyrrocinia CH-67 was all lethal during formulation process. This study provides an effective biocontrol formulation of biofungicide using B. pyrrocinia CH-67 to control tomato leaf mold and damping-off crisphead lettuce and tomato.

• Journal of the Korean Wood Science and Technology
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• 제35권1호
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• pp.1-10
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• 2007

This study was reviewed on the bioethanol production from biomass resources and feedstock supply in America. U.S. Department of Energy (USDE) and the u.s. Department of Agriculture USDA) are both strongly committed to expand the role of biomass as an energy source. They support biomass fuels and products as a way to reduce the need for oil and gas imports, to strengthen the nation's energy security and environmental quality. And it was envisioned a 20 percent replacement of the current U.S.transportation fuel consumption in 2030. Also it was reviewed policies to encourage the expanding of Bio-based fuel use to replace gasoline, such as Clean Air Act, Federal Clean Fuel Program and American Jobs Creation Act. In feedstock supply it was assumed forest biomass will be supplied in 368 million dry tons yearly and the agriculture derived biomass adopted by new technologies and land use change will be supplied in 998 million dry tons, including highly 818 million dry tons of lignocellulosic biomass such as perenial crops (hybrid trees, grasses) corn stover, other crop residues. This amount is 5 times to the amount from based current agricultural technology and crop land.

• Journal of Physiology & Pathology in Korean Medicine
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• 제20권6호
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• pp.1561-1566
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• 2006

Sebum is secreted due to the effect of androgen, which start to be secreted at puberty. Androgens have profound effects on the physiology of the sebaceous gland, Using the human sebocyte cell line SZ95, we investigated the inhibitory effect of Chelidonii Harba (CH) on the subum production. Our results showed that numerous cytoplasmic lipid droplets were examined by Oil red staining and lipid droplets were increased markedly by testosterone. Cell viability was dose-dependently decreased by CH as compared with untreated cells, while total lipid content and cholesterol slightly were increased by CH. Testosterone significantly stimulated the synthesis of total lipid and the synthesis of specific sebaceous lipids such as cholesterol and triglyceride. Combined treatment with CH and testosterone resulted in a lower lipid synthesis than with testosterone alone. Especially cholesteol content was reduced by combined treatment with CH and testosterone. These results indicate that CH inhibits the testosterone-induced lipid synthesis in SZ95 cells and acts antagonistically to androgen at the cellular level.

• Toxicological Research
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• 제26권2호
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• pp.101-108
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• 2010

Halogenated organic compounds, such as 1-bromobutane (1-BB), have been used as cleaning agents, agents for chemical syntheses or extraction solvents in workplace. In the present study, immunotoxic effects of 1-BB and its conjugation with glutathione (GSH) were investigated in female BALB/c mice. Animals were treated orally with 1-BB at 375, 750 and 1500 mg/kg in corn oil once for dose response or treated orally with 1-BB at 1500 mg/kg for 6, 12, 24 and 48 hr for time course. S-Butyl GSH was identified in spleen by liquid chromatography-electrospray ionization tandem mass spectrometry. Splenic GSH levels were significantly reduced by single treatment with 1-BB. S-Butyl GSH conjugates were detected in spleen from 6 hr after treatment. Oral 1-BB significantly suppressed the antibody response to a T-dependent antigen and the production of splenic intracellular interlukin-2 in response to Con A. Our present results suggest that 1-BB could cause immunotoxicity as well as reduction of splenic GSH content, due to the formation of GSH conjugates in mice. The present results would be useful to understand molecular toxic mechanism of low molecular weight haloalkanes and to develop biological markers for exposure to haloalkanes.