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http://dx.doi.org/10.7841/ksbbj.2012.27.6.381

Inhibition of Adipogenesis in 3T3-L1 Adipocytes with Ethanol Extracts of Saururus chinensis  

Shin, Ok-Su (Department of Pathology, Chung-Ang University)
Shin, Youn-Ho (Department of Pathology, Chung-Ang University)
Lee, Kang-Hyun (R&D center, Bioland)
Kim, Gun-Yong (R&D center, Bioland)
Kim, Ki-Ho (R&D center, Bioland)
Park, Jung-Keug (Department of Medical Biotechnology, Dongguk University)
Ahn, Jae-Il (Bio Center, Chungbuk Technopark)
Song, Kye-Yong (Department of Pathology, Chung-Ang University)
Publication Information
KSBB Journal / v.27, no.6, 2012 , pp. 381-386 More about this Journal
Abstract
Obesity increases the risk of many adult diseases, such as atherosclerosis, diabetes, hypertension, ischemic heart disease and breast cancer. Inhibition of adipogenesis is an effective way in the anti-obesity management. Because of main components of Saururus chinensis is flavonoid, it has been showed some improvement by its antioxidant effects on the atherosclerosis, heart disease and diabetic hyperlipidemia. But mechanism of anti-obesity effect of S. chinensis is not clear. We investigated the effects of ethanol extracts of S. chinensis on adipogenesis in the 3T3-L1 pre-adipocyte. The 3T3-L1 cell line is commonly used to study adipogenesis in vitro. In this study, ethanol extracts of S. chinensis significantly decrease the lipid accumulation in the 3T3-L1 cells proved by measuring triglyceride contents and Oil red O staining. The proposed mechanism of inhibition of adipogenesis in the 3T3-L1 cells with ethanol extracts of S. chinensis is down-regulation of transcriptional factors and adipocyte-specific genes such CCAAT/enhancer binding protein ${\alpha}$ ($C/EBP{\alpha}$) and Peroxisome proliferator activated receptor ${\gamma}$ ($PPAR{\gamma}$) in concentration dependent pattern. These results suggest that ethanol extracts of S. chinensis inhibits adipognesis in the 3T3-L1 cells and can be used as a safe and efficient natural substance to manage anti-obesity.
Keywords
3T3-L1; anti-obesity; anti-adipogenesis; Saururus chinensis;
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