• Journal of Microbiology and Biotechnology
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• 제14권6호
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• pp.1327-1332
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• 2004

The hemA gene encoding 5-aminolevulinic acid synthase (ALAS) was cloned from Rhodobacter capsulatus, and its nucleotide sequence was determined. DNA sequencing data revealed one open reading frame coding for a protein with 401 amino acids that displayed high similarity to the amino acid sequences of other known ALASs. The hemA gene was then cloned and expressed under the control of constitutive promotor in E. coli. The recombinant E. coli strain was able to accumulate 5-aminolevulinic acid to 21 mM in the liquid medium supplemented with 45 mM glycine and 120 mM succinate. In addition, a marked effect of the pH of the culture medium on ALA production was observed, and the optimum pH for culture medium was determined to be 5.8-6.3.

• Journal of Microbiology and Biotechnology
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• 제27권5호
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• pp.939-942
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• 2017

The cellular composition and metabolic compounds of Leuconostoc mesenteroides ATCC 8293 were analyzed after cultivation in an anaerobic chemostat. The macromolecular composition was 24.4% polysaccharide, 29.7% protein, 7.9% lipid, 2.9% DNA, and 7.4% RNA. Its amino acid composition included large amounts of lysine, glutamic acid, alanine, and leucine. Elements were in the order of C > O > N > H > S. The metabolites in chemostat culture were lactic acid (73.34 mM), acetic acid (7.69 mM), and mannitol (9.93 mM). These data provide a first view of the cellular composition of L. mesenteroides for use in metabolic flux analysis.

• JSTS:Journal of Semiconductor Technology and Science
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• 제6권1호
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• pp.10-15
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• 2006

Various sizes of erbium/platinum silicided n/p-type Schottky barrier metal-oxide-semiconductor field effect transistors (SB-MOSFETs) are manufactured from $20{\mu}m$ to 10nm. The manufactured SB-MOSFETs show excellent DIBL and subthreshold swing characteristics due to the existence of Schottky barrier between source and channel. It is found that the minimization of trap density between silicide and silicon interface and the reduction of the underlap resistance are the key factors for the improvement of short channel characteristics. The manufactured 10 nm n-type SBMOSFET showed $550{\mu}A/um$ saturation current at $V_{GS}-V_T$ = $V_{DS}$ = 2V condition ($T_{ox}$ = 5nm) with excellent short channel characteristics, which is the highest current level compared with reported data.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2010년도 제39회 하계학술대회 초록집
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• pp.73-73
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• 2010

Various organic- and bio-conjugated nanoparticles have been studied extensively for biological applications in medical diagnoses and drug delivery systems. Gold nanoparticles (AuNP) and poly(ethylene glycol) (PEG) are known biocompatible materials to be used in vivo and are becoming increasingly important in biomedical applications. In this work, we investigated the stability of PEG-conjugated AuNPs, dialysis and centrifuge effects after synthesis or pegylation of AuNPs as a function of elapsed time by using ToF-SIMS imaging technique along with dynamic light scattering (DLS), UV-visible absorption spectroscopic and statistical analyses. Roughly 15-nm-sized AuNPs were synthesized in a citrate-conjugated form, and then converted into the thiol-terminated PEG (O-[2-(3-Mercaptopropionylamino)ethyl]-O'-methylpolyethyleneglycol, M.W.=5 kDa) form. Based on our data, we will show that ToF-SIMS imaging analysis along with DLS, UV-visible absorption and statistical analyses would be a useful method to evaluate stability of PEG-conjugated AuNPs in various environmental conditions.

• 생물환경조절학회지
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• 제23권3호
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• pp.174-180
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• 2014

Standard weather data available to greenhouse environmental design are limited in most regions of the country. So, instead of using standard weather data, in order to find the method to build design weather data for greenhouse heating and cooling, design outdoor weather conditions were analyzed and compared by TAC method and frequency analysis using climatological normal and thirty years from 1981 to 2010 hourly weather data provided by KMA and standard weather data provided by KSES. Average TAC values of outdoor temperature, relative humidity and insolation using thirty years hourly weather data showed a good agreement with them using standard weather data. Therefore, in regions which are not available standard weather data, we suggest that design outdoor weather conditions should be analyzed using thirty years hourly weather data. Average of TAC values derived from every year hourly weather data during the whole period can be established as environmental design standards, and also minimum and maximum of them can be used as reference data.

• 한국지하수토양환경학회지:지하수토양환경
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• 제18권1호
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• pp.46-56
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• 2013

Laboratory scale experiments to remove benzene in solution by using the bio-carrier composed of dead biomass have been performed. The immobilized bio-carrier with dead Bacillus drentensis sp. and polysulfone was manufactured as the biosorbent. Batch sorption experiments were performed with bio-carriers having various quantities of biomass and then, their removal efficiencies and uptake capacities were calculated. From results of batch experiments, 98.0% of the initial benzene (1 mg/L) in 1 liter of solution was removed by using 40 g of immobilized bio-carrier containing 5% biomass within 1 hour and the biosorption reaction reached in equilibrium within 2 hours. Benzene removal efficiency slightly increased (99.0 to $99.4%{\pm}0.05$) as the temperature increased from 15 to $35^{\circ}C$, suggesting that the temperature rarely affects on the removal efficiency of the bio-carrier. The removal efficiency changed under the different initial benzene concentration in solution and benzene removal efficiency of the bio-carrier increased with the increase of the initial benzene concentration (0.001 to 10 mg/L). More than 99.0% of benzene was removed from solution when the initial benzene concentration ranged from 1 to 10 mg/L. From results of fitting process for batch experimental data to Langmuir and Freundlich isotherms, the removal isotherms of benzene were more well fitted to Freundlich model ($r^2$=0.9242) rather than Langmuir model ($r^2$=0.7453). From the column experiment, the benzene removal efficiency maintained over 99.0% until 420 pore volumes of benzene solution (initial benzene concentration: 1 mg/L) were injected in the column packed with bio-carriers, investigating that the immobilized carrier containing Bacillus drentensis sp. and polysulfone is the outstanding biosorbent to remove benzene in solution.

• 한국산업융합학회 논문집
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• 제21권5호
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• pp.247-255
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• 2018

In this research, the effect of normal load, sliding velocity, and texture density on thefriction coefficient of surfaces micro-textured on AISI 4140 under paraffin oil lubrication were investigated. The predicted tribological behavior by numerical calculation can be serves as guidance for the designer during the machine development stage. Therefore, in this research friction coefficient prediction model based on response surface methodology (RSM), support vector machine (SVM), and artificial neural network (ANN) were developed. The experimental result shows that the variation of load, speed and texture density were influence the friction coefficient. The RSM, ANN and SVM model was successfully developed based on the experimental data. The ANN model can effectively predict the tribological characteristics of micro-textured AISI 4140 in paraffin oil lubrication condition compare to RSM and SVM.

• 생물환경조절학회지
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• 제31권3호
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• pp.255-260
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• 2022

본 논문은 딥러닝 알고리즘을 이용하여 딸기 영상 데이터의 병충해 존재 여부를 자동으로 검출할 수 있는 서비스 모델을 제안한다. 또한 병징에 특화된 분할 이미지 데이터 세트를 제안하여 딥러닝 모델의 병충해 검출 성능을 향상한다. 딥러닝 모델은 CNN 기반 YOLO를 선정하여 기존의 R-CNN 기반 모델의 느린 학습속도와 추론속도를 개선하였다. 병충해 검출 모델을 학습하기 위해 일반적인 데이터 세트와 제안하는 분할 이미지 데이터 세트를 구축하였다. 딥러닝 모델이 일반적인 학습 데이터 세트를 학습했을 때 병충해 검출률은 81.35%이며 병충해 검출 신뢰도는 73.35%이다. 반면 딥러닝 모델이 분할 이미지 학습 데이터 세트를 학습했을 때 병충해 검출률은 91.93%이며 병충해 검출 신뢰도는 83.41%이다. 따라서 분할 이미지 데이터를 학습한 딥러닝 모델의 성능이 우수하다는 것을 증명할 수 있었다.

• 한국정보과학회논문지:컴퓨팅의 실제 및 레터
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• 제13권7호
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• pp.433-441
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• 2007

바이오센서는 생명공학 또는 의학 분야에서 사용되는 인간의 생체 신호를 감지할 수 있는 센서들로 의료기기에 주로 사용되는데, 최근 MEMS 기술의 발달로 작은 크기의 하드웨어에 센서 인터페이스, 프로세서, 무선통신, 배터리 등을 포함한 모듈을 센서노드(모트 : Mote)들로 구성된 센서기반 네트워크에서 바이오센서 네트워크로 응용분야를 확장하고 있다. 이에 본 논문에서는 바이오센서 기술과 센서네트워크 기술을 융합한 기술인 바이오 센서네트워크를 활용한 응급 구조 시스템의 설계 및 구현을 제안한다. 제안된 시스템에 사용된 바이오센서는 근전도(EKG), 혈압(Blood Pressure), 맥박(Heart Rate), 산소포화도(Pulse Oximeter), 혈당(Glucose)센서들로, 바이오센서에서 측정된 생체 신호를 센서네트워크 모트를 통해 데이타를 수집하고, 수집된 데이타를 이용하여 건강관리 측정 데이타로 활용하였으며 측정된 데이터는 무선단말기(PDA, 휴대폰), 전자액자 디스플레이장치 등에서 확인 가능하도록 구성하였다. 아울러, 제안한 u- 응급 구조 시스템의 유효성을 실험하기 위해서 사용자의 바이탈사인 정보와 주변 환경정보를 고려한 실험을 수행하였다.

• Journal of Biosystems Engineering
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• 제32권5호
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• pp.324-331
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• 2007

The purpose of this study is to analysis effects of defect factors of combine header for cutting speed of combine header, feeding depth of straw, and cylinder speed of thresher. Measurement system for defect factors was consists of sensors to monitor the combine operation and I/O interface to convert the signals. Cutting speed of combine header, feeding depth of straw, cylinder speed of thresher were measured and analyzed. The data were collected from three paddy field during rice harvesting. The tests were conducted at different grounding speeds, lug troubles, and cutter condition. The one way ANOVA and the multiple comparison tests were performed. The results showed that the measured data were useful to monitor the defect factors of combine during harvesting. The faults conditions of grounding speeds, lug troubles, and cutter conditions affected cutting speeds, feeding depths and cylinder speeds of the combine. The data seem to be useful to analysis the faults conditions of combine header.