• 한국진공학회:학술대회논문집
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• 한국진공학회 2010년도 제39회 하계학술대회 초록집
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• pp.73-73
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• 2010

Various organic- and bio-conjugated nanoparticles have been studied extensively for biological applications in medical diagnoses and drug delivery systems. Gold nanoparticles (AuNP) and poly(ethylene glycol) (PEG) are known biocompatible materials to be used in vivo and are becoming increasingly important in biomedical applications. In this work, we investigated the stability of PEG-conjugated AuNPs, dialysis and centrifuge effects after synthesis or pegylation of AuNPs as a function of elapsed time by using ToF-SIMS imaging technique along with dynamic light scattering (DLS), UV-visible absorption spectroscopic and statistical analyses. Roughly 15-nm-sized AuNPs were synthesized in a citrate-conjugated form, and then converted into the thiol-terminated PEG (O-[2-(3-Mercaptopropionylamino)ethyl]-O'-methylpolyethyleneglycol, M.W.=5 kDa) form. Based on our data, we will show that ToF-SIMS imaging analysis along with DLS, UV-visible absorption and statistical analyses would be a useful method to evaluate stability of PEG-conjugated AuNPs in various environmental conditions.

• 센서학회지
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• 제21권4호
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• pp.270-275
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• 2012

In this study, we have developed a mobile-based visible/NIR(Near InfraRed) imaging equipment for the animal testing. This equipment can provide visible, NIR and merged image through the viewer program. Especially, merged image help researcher to understand visual messages at animal in-vivo test. Also, it is available to send real-time images through the smart phone. Researcher can communicate with another researcher who is a long distance away. Also, the equipment was made with portable small size to enable it to commercialize.

• Journal of the Optical Society of Korea
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• 제15권3호
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• pp.300-304
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• 2011

Optical microscopes are widely used for medical imaging these days, but biopsy is a lengthy process that causes many problems during the ex-vivo imaging procedure. The endo-microscope has been studied to increase accessibility to the human body and to get in-vivo images to use for medical diagnosis. This research proposes a multi-modal confocal endo-microscope for bio-medical imaging. We introduce the design process for a small endoscopic probe and a coupling mechanism for the probe to make the multi-modal confocal endo-microscope. The endoscopic probe was designed to decrease chromatic and spherical aberrations, which deteriorate the images obtained with the conventional GRIN lens. Fluorescence and reflectance images of various samples were obtained with the proposed endo-microscope. We evaluated the performance of the proposed endo-microscope by analyzing the acquired images, and demonstrate the possibilities of in-vivo medical imaging for early diagnosis.

• Biotechnology and Bioprocess Engineering:BBE
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• 제9권2호
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• pp.143-146
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• 2004

A surface plasmon resonance (SPR) imaging system was constructed and used to detect the hexahistidine-ubiquitin-tagged human parathyroid hormone fragment (His$\sub$6/-Ub-hPTHF(1-34)) expressed in Escherichia coli. The hexahistidine-specific antibody was immobilized on a thin gold film coated with ProLinker$\^$TM/ B, a novel calixcrown derivative with a bifunctional coupling property that permits efficient immobilizaton of capture proteins on solid matrices. The soluble and insoluble fractions of an E. coli cell lysate were spotted onto the antibody-coated gold chip, which was then washed with buffer (pH 7.4) solution and dried. SPR imaging measurements were carried out to detect the expressed His$\sub$6/-Ub-hPTHF(1-34). There was no discernible protein image in the uninduced cell lysate, indicating that non-specific binding of contaminant proteins did not occur on the gold chip surface. It is expected that the approach used here to detect affinity-tagged recombinant proteins using an SPR imaging technique could be used as a powerful tool for the analyses of a number of proteins in a high-throughput mode.

• 대한의용생체공학회:의공학회지
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• 제26권5호
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• pp.283-294
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• 2005

The Center for Imaging Human Structures (CIH) was established in December 2002 to develop new diagnostic imaging techniques and to make them available to the greater community of biomedical and clinical researchers at Sungkyunkwan University. CIH has been involved in 5 specific activities to provide solutions for early diagnosis and improved treatment of human diseases. The five area goals include: 1) development of a digital mammography system with computer aided diagnosis (CAD); 2) development of digital radiological imaging techniques; 3) development of unified medical solutions using 3D image fusion; 4) development of multi-purpose digital endoscopy; and, 5) evaluation of new imaging systems for clinical application

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVII)
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• pp.770-770
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• 2005

• 대한의용생체공학회:의공학회지
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• 제44권4호
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• pp.255-263
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• 2023

Clinical ultrasound (US) is a widely used imaging modality with various clinical applications. However, capturing a large field of view often requires specialized transducers which have limitations for specific clinical scenarios. Panoramic imaging offers an alternative approach by sequentially aligning image sections acquired from freehand sweeps using a standard transducer. To reconstruct a 3D volume from these 2D sections, an external device can be employed to track the transducer's motion accurately. However, the presence of optical or electrical interferences in a clinical setting often leads to incorrect measurements from such sensors. In this paper, we propose a deep learning (DL) framework that enables the prediction of scan trajectories using only US data, eliminating the need for an external tracking device. Our approach incorporates diverse data types, including correlation volume, optical flow, B-mode images, and rawer data (IQ data). We develop a DL network capable of effectively handling these data types and introduce an attention technique to emphasize crucial local areas for precise trajectory prediction. Through extensive experimentation, we demonstrate the superiority of our proposed method over other DL-based approaches in terms of long trajectory prediction performance. Our findings highlight the potential of employing DL techniques for trajectory estimation in clinical ultrasound, offering a promising alternative for panoramic imaging.

• Imaging Science in Dentistry
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• 제31권4호
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• pp.235-240
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• 2001

Purpose: To evaluate displacement and morphologic changes of the temporomandibular joint (TMJ) disc in patient with internal derangement using magnetic resonance imaging (MRI). Materials and Methods: One hundred and forty five MR images of TMJs in 73 patients were evaluated. Positional and morphologic changes of the TMJ discs were assessed. Lateral or medial disc displacement was also evaluated on coronal images. Results: Among 63 discs with anterior disc displacement, 37 discs were assessed as a biconcave disc and 21 as a deformed disc. Rotational disc displacement was observed in 35 discs. Anteromedial disc displacement was observed in 29 discs, and anterolateral direction in 6 discs. Among 35 rotational displacement, 5 biconcave discs and 21 deformed discs were observed. Conclusion : Rotational and sideways displacement of TMJ discs were found to be common and an important aspect of internal derangement. This study also suggests that sagittal and coronal images of the TMJ have complementary abilities for an assessment of joint abnormality.

• Nuclear Medicine and Molecular Imaging
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• 제43권4호
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• pp.344-351
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• 2009

목적: 광학영상기술은 소동물이나 임상연구에서 분자영상법으로 알려진 첨단연구 분야이다. 광학영상기기는 소동물영상연구 및 추적연구에 중요한 역할을 수행하고 있다. 발광영상에서 소동물을 영상화 하기 위해서는 피부조직을 뚫고 나오는 광자를 검출하기 위한 고민감도 CCD카메라가 필요하다. 이 연구에서는 소동물에서 발생하는 발광신호를 검출하기 위해 개발한 광학영상기기를 소개하고자 한다. 대상 및 방법: 냉각형 CCD카메라와 집광렌즈, 8개의 백색광 LED광원을 암실상자 안에 장치하였다. 팬텀 및 튜브를 이용한 영상을 얻은 후 발광 박테리아를 이용하여 CT26 암모델 누드마우스에서 영상을 획득하였다. 결과: 발광영상을 얻기 위한 광학영상기기를 설계하고 개발하였다. 영상획득이 성공적으로 수행되었고, 시스템을 완성하였다. 개발된 장비는 분자영상연구에 사용되고 있다. 결론 개발된 광학영상장비는 다양한 실험적 조건을 만족하는 연구에 최적화하여 유용한 도구로 자리잡을 것으로 기대한다.

• Applied Microscopy
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• 제45권3호
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• pp.150-154
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• 2015

Negative staining electron microscopy facilitates the visualization of small bio-materials such as proteins; thus, many electron microscopists have used this conventional method to visualize the morphologies and structures of biological materials. To achieve sufficient contrast of the materials, a number of imaging parameters must be considered. Here, we examined the effects of one of the fundamental imaging parameters, electron beam exposure time, on electron densities generated using transmission electron microscopy. A single site of a negatively stained biological sample was illuminated with the electron beam for different times (1, 2, or 4 seconds) and sets of micrographs were collected. Computational image processing demonstrated that longer exposure times provide better electron densities at the molecular level. This report describes technical procedures for testing parameters that allow enhanced evaluations of the densities of electron microscopy images.