• Journal of Ginseng Research
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• 제40권4호
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• pp.315-324
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• 2016

Background: Biocontrol agents are regarded as promising and environmental friendly approaches as agrochemicals for phytodiseases that cause serious environmental and health problems. Trichoderma species have been widely used in suppression of soil-borne pathogens. In this study, an endophytic fungus, Trichoderma gamsii YIM PH30019, from healthy Panax notoginseng root was investigated for its biocontrol potential. Methods: In vitro detached healthy roots, and pot and field experiments were used to investigate the pathogenicity and biocontrol efficacy of T. gamsii YIM PH30019 to the host plant. The antagonistic mechanisms against test phytopathogens were analyzed using dual culture, scanning electron microscopy, and volatile organic compounds (VOCs). Tolerance to chemical fertilizers was also tested in a series of concentrations. Results: The results indicated that T. gamsii YIM PH30019 was nonpathogenic to the host, presented appreciable biocontrol efficacy, and could tolerate chemical fertilizer concentrations of up to 20%. T. gamsii YIM PH30019 displayed antagonistic activities against the pathogenic fungi of P. notoginseng via production of VOCs. On the basis of gas chromatography-mass spectrometry, VOCs were identified as dimethyl disulfide, dibenzofuran, methanethiol, ketones, etc., which are effective ingredients for antagonistic activity. T. gamsii YIM PH30019 was able to improve the seedlings' emergence and protect P. notoginseng plants from soil-borne disease in the continuous cropping field tests. Conclusion: The results suggest that the endophytic fungus T. gamsii YIM PH30019 may have a good potential as a biological control agent against notoginseng phytodiseases and can provide a clue to further illuminate the interactions between Trichoderma and phytopathogens.

• 한국습지학회지
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• 제26권3호
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• pp.219-226
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• 2024

생태계를 보호하고 생태계적 가치를 증진하기 위해 생태계서비스지불제(Payment for Ecosystem Services, PES)가 강조되고 있다. 그러나 PES 시행과 정책의 효과적인 실행을 위해 필요한 생태계 모니터링과 연구는 부족한 상황이다. 이에 본 연구는 장항습지에서 시행 중인 PES의 효과를 분석하여 서식지 관리 방안을 제시하였으며, 이때 이동성 조류 모니터링과 주요 종의 먹이원을 분석하였다. 장항습지에서 관찰된 우점 조류로는 괭이갈매기, 청둥오리, 쇠기러기 등이 있으며, 이들의 주요 먹이원은 참새귀리, 개보리 등의 벼과 식물과 속속이풀 등 십자화과 식물로 분석되었다. 또한 PES를 통해 장항습지 내에서 볍씨 공급이 이루어지고 있으며, 재두루미, 큰기러기, 쇠기러기 등의 조류가 이를 이용하고 있다는 결과가 관찰되었다. 장항습지의 지속 가능한 관리를 위해 먹이 공급 시스템 개선과 재두루미 및 기러기류(큰기러기, 쇠기러기)에 대한 분산 공급 방안을 모색해야 한다. 이때, 농업 및 개발 지역과의 경계를 관리하여 생태적 연결성을 강화해야 한다. 본 연구에서는 이동성 조류의 서식지로서 장항 습지의 생태적 중요성을 재확인하였다. 또한, 이들 결과는 향후 정책 수립과 지속 가능한 보전을 위한 기초 자료로 활용될 수 있는 의의를 갖는다.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2010년도 심포지엄 및 추계학술발표회
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• pp.155-156
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• 2010

• 한국응용곤충학회지
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• 제54권4호
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• pp.335-343
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• 2015

유전자변형 작물이 절지동물에 미칠 수 있는 잠재적인 부정적 영향은 유전자변형 작물의 주요한 환경위해성의 하나로 여겨지고 있다. 본 연구에서는 PPO (protoporphyrinogen oxidase) 저해 제초제 내성 유전자변형 벼가 절지동물에 미칠 수 있는 영향을 평가하기 위하여 절지동물의 다양성과 군집구조를 조사하였다. 절지동물은 야외포장에서 벼의 생육기간 동안 황색점착트랩을 이용하여 채집하였다. 유전자변형 벼는 채집된 절지동물군집의 다양도 지수에 유의한 영향을 주지 않았다. 또한 다변량분석(PerMANOVA, NMDS) 결과에서도 절지동물군집 구조는 채집시기에 따라 달랐지만 벼의 유전형(유전자변형 또는 비변형)에 의해 영향을 받지 않았다.

• 한국약용작물학회지
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• 제21권3호
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• pp.220-228
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• 2013

This study was conducted to assess the genetic diversity and population structure of 70 amaranth accessions collected from South and Southeast Asia using 14 simple sequence repeat (SSR) markers. In total, 67 alleles were detected, with an average of 4.79 per locus. Rare alleles comprised a large portion (46.3%) of the detected alleles, and 29 unique alleles associated with rice accessions were also discovered. The mean major allele frequency (MAF), genetic diversity (GD) and polymorphic information content (PIC) of the 14 SSR loci were 0.77, 0.36, and 0.34, respectively. A model-based structural analysis revealed the presence of three subpopulations. The genetic relationships revealed by the neighbor-joining tree method were fairly consistent with the structure-based membership assignments for most of the accessions. All 70 accessions showed a clear relationship to each cluster without any admixtures. We observed a relatively low extent of genetic exchange within or among amaranth species from South and Southeast Asia. The genetic diversity results could be used to identify amaranth germplasms and so facilitate their use for crop improvement.

• Journal of Microbiology
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• 제45권2호
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• pp.105-112
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• 2007

The objective of this study was to analyze the phylogenetic composition of bacterial community in the soil of an earth-cave (Niu Cave) using a culture-independent molecular approach. 16S rRNA genes were amplified directly from soil DNA with universally conserved and Bacteria-specific rRNA gene primers and cloned. The clone library was screened by restriction fragment length polymorphism (RFLP), and representative rRNA gene sequences were determined. A total of 115 bacterial sequence types were found in 190 analyzed clones. Phylogenetic sequence analyses revealed novel 16S rRNA gene sequence types and a high diversity of putative bacterial community. Members of these bacteria included Proteobacteria (42.6%), Acidobacteria (18.6%), Planctomycetes (9.0 %), Chloroflexi (Green nonsulfur bacteria, 7.5%), Bacteroidetes (2.1%), Gemmatimonadetes (2.7%), Nitrospirae (8.0%), Actinobacteria (High G+C Gram-positive bacteria, 6.4%) and candidate divisions (including the OP3, GN08, and SBR1093, 3.2%). Thirty-five clones were affiliated with bacteria that were related to nitrogen, sulfur, iron or manganese cycles. The comparison of the present data with the data obtained previously from caves based on 16S rRNA gene analysis revealed similarities in the bacterial community components, especially in the high abundance of Proteobacteria and Acidobacteria. Furthermore, this study provided the novel evidence for presence of Gemmatimonadetes, Nitrosomonadales, Oceanospirillales, and Rubrobacterales in a karstic hypogean environment.

• Journal of Species Research
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• 제10권2호
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• pp.99-116
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• 2021

In 2018, as a subset study to discover indigenous prokaryotic species in Korea, a total of 18 unreported bacterial strains were discovered. From the high 16S rRNA gene sequence similarity (>98.8%) and formation of a robust phylogenetic clade, it was determined that each strain belonged an independent and predefined bacterial species. There were no official report that these 18 species were previously described in Korea; therefore, one strain of Williamsia, one strain of Rhodococcus, three strains of Microbacterium, three strains of Agromyces, one strain of Arthrobacter, one strain of Paeniglutamicibacter, one strain of Pseudarthrobacter, one strain of Nocardioides, one strain of Fibrella, one strain of Hymenobacter, one strain of Deinococcus, two strains of Fictibacillus, and one strain of Paenibacillus are described as unreported bacterial species in Korea. Gram reaction, basic biochemical characteristics, and colony and cell morphologies are described in the species description section.

• Journal of Species Research
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• 제10권4호
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• pp.321-335
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• 2021

In 2020, a total of 12 bacterial strains were isolated from soil after a comprehensive investigation of indigenous prokaryotic species in Korea. It was determined that each strain belonged to independent and predefined bacterial species, with high 16S rRNA gene sequence similarity (>98.7%) and formation of a robust phylogenetic clade with the closest species. This study identified four families in the phylum Actinobacteria, two families in the phylum Proteobacteria, one family in the phylum Bacteroidetes one family in the phylum Firmicutes; and four species in the family Nocardiaceae, two species in the family Nocardioidaceae, one species in the family Cellulomonadaceae, one species in the family Hymenobacter, one species in the family Methylobacteriaceae, one species in the family Microbacteriaceae, one species in the family Bacillaceae and one species in the family Sphingomonadaceae. There is no official report of these 12 species in Korea, so they are described as unreported bacterial species in Korea in this study. Gram reaction, basic biochemical characteristics, colony, and cell morphology are included in the species description section.

• Journal of Forest and Environmental Science
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• 제30권1호
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• pp.91-100
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• 2014

This research was conducted on the conservation area of Baekdudaegan, Kangwondo under the purpose of evaluating bio-diversity according to the changes of spatial scale, using GIS data and spatial filtering method. The diversity index was calculated based on the information of species of The $5^{th}$ forest type map using Shannon-weaver index (H'), evenness index ($E_i$) and richness index ($R_i$). The diversity index was analyzed and compared according to the changes of 12 spatial scales from Kernel size $3{\times}3$ to $73{\times}73$ and basin unit. As for H' and $R_i$, spatial scale increased as diversity index decreased, while $E_i$ decreases gradually. H' and $R_i$ was highest; each 1.1 and 0.6, when the Kernel size was $73{\times}73$, while $E_i$ was 0.2, the lowest. When you look at according to the basin unit, for large basin unit, 'YeongDong' region shows higher diversity index than 'YeongSeo' region. For middle basin unit, 'Gangneung Namdaecheon' region, and for small basin unit, 'Gangneung Namdaecheon' and 'Gangneung Ohbongdaem' region shows high diversity index. When you look at the relationship between diversity index and Geographic factors, H' shows positive relation to curvature and sunshine factor while shows negative to elevation, slope, hillshade, and wetness index. Also $E_i$ was similar to the relationship between H' and Geographic factor. Meanwhile, $R_i$ shows positive relationship to curvature and sunshine factor, while negative to elevation, slope, hillshade, and wetness index. macro unit diversity index evaluation was possible through the GIS data and spatial filtering, and it can be a good source for local biosphere conservation policy making.

• Journal of Applied Biological Chemistry
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• 제48권3호
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• pp.113-119
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• 2005

O-methylation mediated by O-methyltransferases (OMTs) is a common modification in natural product biosynthesis and contributes to diversity of secondary metabolites. OMTs use phenylpropanoids, flavonoids, other phenolics and alkaloids as substrates, and share common domains for S-adenosyl-L-methionine (AdoMet) and substrate binding. We searched Arabiposis genome and found 17 OMTs genes (AtOMTs). AdoMet- and substrate-binding sites were predicted. AdoMet binding domain of AtOMTs is highly conserved, while substrate-binding domain is diverse, indicating use of different substrates. In addition, expressions of six AtOMT genes in response to UV and in different tissues were investigated using real-time quantitative reverse transcriptase-polymerase chain reaction. All the AtOMTs investigated were expressed under normal growth condition and most, except AtOMT10, were induced after UV illumination. AtOMT1 and AtOMT8 were expressed in all the tissues, whereas AtOMT10 showed flower-specific expression. Analysis of these AtOMT gene expressions could provide some clues on AtOMT involvement in the cellular processes.