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Instrumentation and Software for Analysis of Arabidopsis Circadian Leaf Movement

  • Kim, Jeong-Sik;Nam, Hong-Gil
    • Interdisciplinary Bio Central
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    • v.1 no.1
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    • pp.5.1-5.4
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    • 2009
  • This article is an addendum to the authors’ previous article (Kim, J. et al. (2008) Plant Cell 20, 307-319). The instrumentation and software described in this article were used to analyze the circadian leaf movement in the previous article. Here, we provide detailed and practical information on the instrumentation and the software. The source code of the LMA program is freely available from the authors. The circadian clock regulates a wide range of cyclic physiological responses with a 24 hour period in most organisms. Rhythmic leaf movement in plants is a typical robust manifestation of rhythms controlled by the circadian clock and has been used to monitor endogenous circadian clock activity. Here, we introduce a relatively easy, inexpensive, and simple approach for measuring leaf movement circadian rhythms using a USB-based web camera, public domain software and a Leaf Movement Assay (LMA) program. The LMA program is a semi-automated tool that enables the user to measure leaf lengths of individual Arabidopsis seedlings from a set of time-series images and generates a wave-form output for leaf rhythm. This is a useful and convenient tool for monitoring the status of a plant's circadian clock without an expensive commercial instrumentation and software.

Separation characteristics of particles in a self-rotating type centrifugal oil purifier

  • Pyo, Young-Seok;Jung, Ho-Yun;Choi, Yoon-Hwan;Doh, Deog-Hee;Lee, Yeon-Won
    • Journal of Advanced Marine Engineering and Technology
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    • v.38 no.2
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    • pp.147-153
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    • 2014
  • The centrifugal oil purifier is used in an engine for lubrication and to remove impurities. The momentum needed for the rotation of the cylindrical chamber is obtained by jet injections. An impure particle in the oil is separated by the centrifugal forces moving to the inner wall of the rotating cylindrical chamber body. The dust particles are eliminated when the particles are absorbed onto the surface of the inner wall of the chamber body. The flow characteristics and the physical behaviors of particles in this centrifugal oil purifier were investigated numerically and the filtration efficiencies was evaluated. For calculations, a commercial code is used and the SST (Shear Stress Transport) turbulence model has been adopted. The MFR (Multi Frames of Reference) method is introduced to consider the rotating effect of the flows. Under various variables, such as particle size, particle density and rotating speed, the filtration efficiencies are evaluated. It has been verified that the filtration efficiency is increased with the increments in the particle size, the particle density and the rotating speed of the cylindrical chamber.

Research on the nasal airflow and heat and mass transfer (비강 내 공기유동과 열 및 물질전달에 관한 연구)

  • Kim, Sung-Kyun;Liem, Huynh Quang;Park, Joon-Hyung
    • Proceedings of the KSME Conference
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    • 2008.11a
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    • pp.1479-1483
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    • 2008
  • The three main physiological functions of nose are air-conditioning, filtering and smelling. Knowledge of airflow characteristics in nasal cavities is essential to understand the physiological and pathological aspects of nasal breathing. Several studies have utilized physical models of the healthy nasal cavity to investigate the relationship between nasal anatomy and airflow. In our laboratory, there have been a series of experimental investigations on the nasal airflow in normal and deformed nasal cavity models by PIV under both constant and periodic flow conditions. In this time, airflow inside normal nasal cavity is investigated numerically by the FVM general purpose code. The comparisons with PIV measurement are appreciated. Heat and humidity transfer is dealt numerically. Dense CT data and careful treatment of model surface under the ENT doctor’s advice provide more sophisticated cavity models for both PIV experiment and numerical grid system. Average and RMS velocity distributions have been obtained for inspirational and expirational nasal. Temperature distribution, heat and humidity transfer through the mucosa are obtained.

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Development of Cyclone Conveying System for Transporting Chopped Round Bale Roughage and Separating Dust (흙먼지 분리기 용이한 원형베일 세절 조사료의 사이클론 이송장치 개발)

  • Ha, Yu-Shin;Kwon, Jin-Kyung;Park, Kyung-Kyoo
    • Journal of Biosystems Engineering
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    • v.36 no.3
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    • pp.204-210
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    • 2011
  • This study developed the cyclone conveying system using roughage cutter for the round bale reported in the previous papers. Performance tests were conducted whether it can easily separate dust from roughage such as rice straws and it can transport roughages from cutter to TMR mixer. In addition, the airflow patterns in the cyclone conveying system were investigated using CFD code (FLUENT 6.2) for various velocities of dust collection fan. The cyclone conveying system was designed based on dried rice straws with a diameter of 1,340 mm, a cylinder length of 1,220 mm, a cone length of 850 mm and the current velocity of the dust collection fan was 15~20 m/s. It was found that transporting of roughage from cutter to TMR mixer and the separation of dust were satisfactory, and the dust removal rate of rice straws was around 31.9%. CFD analysis showed that, at the blowing fan velocity of 11.6 m/s, the airflow velocity inside the dust collector increased as velocity of the dust collection fan increased, but the airflow patterns inside the dust collector were all much the same.

Field Survey and Analysis of Natural Ventilation Characteristics of Multi-span Greenhouse with Different Roof Vent (연동형 비닐하우스의 환기창 형태 조사 및 자연환기 효과 분석)

  • Park, Min jung;Choi, Duck kyu;Son, Jin kwan;Yoon, Sung-Wook;Kim, Hee tae;Lee, Seung-Kee;Kang, Dong hyeon
    • Journal of Bio-Environment Control
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    • v.29 no.1
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    • pp.36-42
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    • 2020
  • The objectives of this study were to investigate the standard and roof vent type of multi-span greenhouse and to analyze the characteristics of natural ventilation of multi-span greenhouse with different roof vent using computational fluid dynamics (CFD) code. The vent area proportion of surveyed farms averaged 10%, it was analyzed that the vent design for natural ventilation is insufficient. The results of natural ventilation efficiency of multi-span greenhouse according to roof vent type showed that the temperature of the position in which the crops grew was the lowest in the conical roof vent type and the highest in the half conical roof vent type. For the natural ventilation effect, the conical roof vent type was the best one, but the structural stability should be evaluated in light of wind load.

Quality Properties of Honey in Korean Commercial Markets (국내 유통되는 벌꿀의 품질특성)

  • Kim, Jae-Young;Song, Ha-Yeon;Moon, Jin-Ah;Shin, Min-Hong;Baek, Seung-Hwa
    • Korean Journal of Food Science and Technology
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    • v.46 no.4
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    • pp.432-437
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    • 2014
  • This study was carried out to examine the quality properties of honey in Korean commercial markets. The moisture content, stable carbon isotope ratio, invert sugar, cane sugar, and hydroxy-methylfurfural (HMF) contents of honey were measured according to the Korea Food Code and AOAC's (Association of Official Analytical Chemists) official methods. The stable carbon isotope ratio ranged from -25.18‰ to -12.60‰, which clearly differed between honey of $C_3$ origin (flower) and $C_4$ origin (artificial). Results of quality measurements revealed a moisture content of 18.12 to 19.70%, fructose content of 36.10 to 43.94%, glucose content of 22.61 to 31.91%, sucrose content of 1.56 to 4.75%, invert sugar content of 64.89 to 72.79%, and HMF content of 4.10 to 78.66 mg/kg. These values demonstrate that the quality of the tested honey meets the standard criteria of the Korean Food Code and Codex. However, it is necessary to reconsider the appropriate criteria for imported honey because it is circulated in the market through a long distribution process.

An Approach for a Substitution Matrix Based on Protein Blocks and Physicochemical Properties of Amino Acids through PCA

  • You, Youngki;Jang, Inhwan;Lee, Kyungro;Kim, Heonjoo;Lee, Kwanhee
    • Interdisciplinary Bio Central
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    • v.6 no.4
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    • pp.3.1-3.10
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    • 2014
  • Amino acid substitution matrices are essential tools for protein sequence analysis, homology sequence search in protein databases and multiple sequence alignment. The PAM matrix was the first widely used amino acid substitution matrix. The BLOSUM series then succeeded the PAM matrix. Most substitution matrixes were developed by using the statistical frequency of substitution between each amino acid at blocks representing groups of protein families or related proteins. However, substitution of amino acids is based on the similarity of physiochemical properties of each amino acid. In this study, a new approach was used to obtain major physiochemical properties in multiple sequence alignment. Frequency of amino acid substitution in multiple sequence alignment database and selected attributes of amino acids in physiochemical properties database were merged. This merged data showed the major physiochemical properties through principle components analysis. Using factor analysis, these four principle components were interpreted as flexibility of electronic movement, polarity, negative charge and structural flexibility. Applying these four components, BAPS was constructed and validated for accuracy. When comparing receiver operated characteristic ($ROC_{50}$) values, BAPS scored slightly lower than BLOSUM and PAM. However, when evaluating for accuracy by comparing results from multiple sequence alignment with the structural alignment results of two test data sets with known three-dimensional structure in the homologous structure alignment database, the result of the test for BAPS was comparatively equivalent or better than results for prior matrices including PAM, Gonnet, Identity and Genetic code matrix.

InterCom : Design and Implementation of an Agent-based Internet Computing Environment (InterCom : 에이전트 기반 인터넷 컴퓨팅 환경 설계 및 구현)

  • Kim, Myung-Ho;Park, Kweon
    • The KIPS Transactions:PartA
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    • v.8A no.3
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    • pp.235-244
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    • 2001
  • Development of network and computer technology results in many studies to use physically distributed computers as a single resource. Generally, these studies have focused on developing environments based on message passing. These environments are mainly used to solve problems for scientific computation and process in parallel suing inside parallelism of the given problems. Therefore, these environments provide high parallelism generally, while it is difficult to program and use as well as it is required to have user accounts in the distributed computers. If a given problem is divided into completely independent subproblems, more efficient environment can be provided. We can find these problems in bio-informatics, 3D animatin, graphics, and etc., so the development of new environment for these problems can be considered to be very important. Therefore, we suggest new environment called InterCom based on a proxy computing, which can solve these problems efficiently, and explain the implementation of this environment. This environment consists of agent, server, and client. Merits of this environment are easy programing, no need of user accounts in the distributed computers, and easiness by compiling distributed code automatically.

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Web-Based Data Processing and Model Linkage Techniques for Agricultural Water-Resource Analysis (농촌유역 물순환 해석을 위한 웹기반 자료 전처리 및 모형 연계 기법 개발)

  • Park, Jihoon;Kang, Moon Seong;Song, Jung-Hun;Jun, Sang Min;Kim, Kyeung;Ryu, Jeong Hoon
    • Journal of The Korean Society of Agricultural Engineers
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    • v.57 no.5
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    • pp.101-111
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    • 2015
  • Establishment of appropriate data in certain formats is essential for agricultural water cycle analysis, which involves complex interactions and uncertainties such as climate change, social & economic change, and watershed environmental change. The main objective of this study was to develop web-based Data processing and Model linkage Techniques for Agricultural Water-Resource analysis (AWR-DMT). The developed techniques consisted of database development, data processing technique, and model linkage technique. The watershed of this study was the upper Cheongmi stream and Geunsam-Ri. The database was constructed using MS SQL with data code, watershed characteristics, reservoir information, weather station information, meteorological data, processed data, hydrological data, and paddy field information. The AWR-DMT was developed using Python. Processing technique generated probable rainfall data using non-stationary frequency analysis and evapotranspiration data. Model linkage technique built input data for agricultural watershed models, such as the TANK and Agricultural Watershed Supply (AWS). This study might be considered to contribute to the development of intelligent watercycle analysis by developing data processing and model linkage techniques for agricultural water-resource analysis.

A Study on integrated water management system based on Web maps

  • Choi, Ho Sung;Jung, Jin Young;Park, Koo Rack
    • Journal of the Korea Society of Computer and Information
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    • v.21 no.8
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    • pp.57-64
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    • 2016
  • Initial prevention activities and rapid propagation conditions is the most important to prevent diffusion of water pollution. If water pollutants flow into streams river or main stresm located in environmental conservation area or water intake facilities, we must predict immediately arrival time and the diffusion concentration to the proactive. National Institute of Environmental Research developed water pollution incident response prediction system linking dam and movable weir. the system is mathematical model which is updated daily. Therefore it can quickly predict the arrival time and the diffusion concentration when there are accident of oil spills and hazardous chemicals. Also we equipped with mathematical model and toxicity model of EFDC(Environmental Fluid Dynamics Code) to calculate the arrival time and the diffusion concentration. However these systems offer the services of an offline manner than real-time control services. we have ensured the reliability of data collection and have developed a real-time water quality measurement data transmission device by using the data linkage utilizing a mode bus communication and a commercial SCADA system, in particular, we implemented to be able to do real-time water quality prediction through information infrastructure of the water quality integrated management business created by utilizing the construction of the real-time prediction system that utilizes the data collected, the Open map, the visual representation using charts API and development of integrated management system development based on web maps.