• Title/Summary/Keyword: Bio System

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Effect of Dietary Microbial Phytase on Laying Performance, Egg Quality, Phosphorus Utilization and Nutrient Metabolizability in Laying Hens (산란계에 Microbial Phytase 첨가시 산란율, 계란 품질, 인 이용율 및 영양소 대사율에 미치는 영향)

  • Jang, H.D.;Hyun, Y.;Kim, H.S.;Hwang, I.W.;Yoo, J.S.;Kim, H.J.;Shin, S.O.;Hwang, Y.;Zhou, T.X.;Chen, Y.J.;Cho, J.H.;Kim, I.H.
    • Korean Journal of Poultry Science
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    • v.35 no.2
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    • pp.115-121
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    • 2008
  • The study was conducted to evaluate the effects of dietary microbial phytase on egg productivity, egg quality, phosphorus utilization and nutrient digestibility in laying hens. The animals used in the experiment were a total of 120 Hy-Line Brown laying hens (32 weeks old). Dietary treatments included 1) CON (basal diet), 2) LP (low phosphorus diet) 3) NP (low phosphorus diet + 0.03% normal microbial phytase) and 4) CP (low phosphorus diet + 0.03% coated microbial phytase). Six laying hens were allotted to a block (pen) with five replicated. Through the whole period of experiment, egg production was significantly increased in CON and CP treatments compared to LP treatment (P<0.05). CON, CP and NP treatments significantly increased their egg shell breaking strength and egg shell thickness compared to LP treatment (P<0.05). CON, CP and NP treatments resulted higher yolk color and Haugh unit than LP treatment (P<0.05). Calcium and inorganic phosphorus contents in blood were higher in CP treatment compared to LP treatment (P<0.05). Dry matter digestibility and nitrogen digestibility were greater in CP treatment than CON, LP and NP treatments (P<0.05). Crude ash was increased in CON, CP and NP treatments compared to LP treatment (P<0.05). Calcium digestibility and phosphorus digestibility were significantly improved in CP and NP treatments than CON and LP treatments (P<0.05). Calcium retention of CON, CP and NP treatments were higher than LP treatment (P<0.05). Phosphorus retention was increased in CP and NP treatments compared to CON and LP treatments (P<0.05). LP treatment significantly increased their nitrogen, calcium excretion compared to CON, CP and NP treatments (P<0.05). Phosphorus excretion was decreased in LP, CP and NP treatments compared to CON treatment (P<0.05). In conclusion, CP treatment improved egg production, egg quality, inorganic calcium and phosphorus in blood, calcium and phosphorus retention and nutrient digestibility and decreased nitrogen and calcium excretion in laying hens.

Monitoring Bacillus cereus and Aerobic Bacteria in Raw Infant Formula and Microbial Quality Control during Manufacturing (영.유아용 식품원료의 Bacillus cereus와 일반세균 모니터링 및 제조공정 중 미생물 품질제어)

  • Jung, Woo-Young;Eom, Joon-Ho;Kim, Byeong-Jo;Ju, In-Sun;Kim, Chang-Soo;Kim, Mi-Ra;Byun, Jung-A;Park, You-Gyoung;Son, Sang-Hyuck;Lee, Eun-Mi;Jung, Rae-Seok;Na, Mi-Ae;Yuk, Dong-Yeon;Gang, Ji-Yeon;Heo, Ok-Sun;Yoon, Min-Ho
    • Korean Journal of Food Science and Technology
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    • v.42 no.4
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    • pp.494-501
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    • 2010
  • The purpose of this study was to examine the presence of Bacillus cereus, aerobic bacteria and coliforms in the raw material of infant formulas and investigate the manufacturing process in terms of microbial safety. Among ten kinds of raw infant formula material samples (n=20), Bacillus cereus appeared in two (n=4). Aerobic bacteria were not detected in raw infant formula material or maximum 4.15 log CFU/g. Eleven species of aerobic bacteria were isolated and 76% of them were Sphingomonas paucimobilis, Pseudomonas fluorescens, Rhizobium radiobactor, or Stenotrophomonas maltophilia. A Pearson's correlation analysis revealed that the most influential factors for detecting Bacillus cereus were aerobic bacteria and coliforms. In other words, when the measured values of aerobic bacteria and coliforms were higher, the possibility that Bacillus cereus would appear increased. In a regression model to predict Bacillus cereus, the rate of appearance was correlated with aerobic bacteria and coliforms, and its contribution rate for effectiveness was 86%. Improving microbial quality control by pasteurization, spray dry, popping and extrusion resulted in a decrease in the numbers of Bacillus cereus, aerobic bacteria and coliforms in the raw materials. The results suggest that a hazard analysis and critical control point system might be effective for reducing microbiological contamination.

Evaluating the Visual Contrast Rating of the Landscape Impact Factors - A case study for the Buildings in Natural Landscape - (경관영향 요소별 경관 대비성 평가 - 자연경관에 도입되는 건축물을 중심으로 -)

  • Choi, Won-Bin;Shin, Ji-Hoon
    • Journal of Korean Society of Rural Planning
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    • v.24 no.3
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    • pp.87-96
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    • 2018
  • While cities became bigger and bigger since 1990s, many indiscretely high buildings started to be built in the mountain areas inside a city and in the rural areas in the suburb of each city. To regulate such indiscrete developments, the government prepared for some relevant legal and institutional criteria by having enacted the "Landscape Act" and established a strong management means in the legal and institutional aspects by having introduced the natural landscape deliberation system and the landscape deliberation one into the "Natural Environment Conservation Act." However, since some uniform levels of absolute height and no. of stories are suggested legally and institutionally, it is hard to consider the effects of a real building structure onto the relevant landscape. Accordingly, this thesis is intended to grasp the contrast of the landscape elements in the allowable height section, which is presented through landscape sensitivity. As the results from the visual contrast rating on a small apartment complex located in Dangjin and a large scale of apartment complex in Seosan as the apartment complexes surrounded with natural landscapes that were selected as the subjects of this study, the following conclusion can be finalized. First, there were deducted some factors, that is, forms, lines, colors, textures and sizes as the ones with which can measure and evaluate the contrasting properties when a structure gets into a natural landscape. Second, in case of a small scale of apartment complex (in the foreground) compared to a large one (in the foreground), it was found that the contrasting properties were bigger. In addition, it was also found that the contrasting property of the landscape factor of the foreground compared to that of the middle one becomes bigger depending on a distance. Third, as the results from an evaluation on the contrasting properties of the landscape factor depending on the changes of each floor of a structure, it was found that the factors, that is, forms, lines, colors, textures and sizes are very significant. Among those factors, the factors, forms and lines in a small scale of apartment complex (in the foreground) showed each log regression. But in all of the other cases, they showed each line regression. Fourth, as the results from examining the regression coefficients of the landscape factor, the coefficients of the shapes and lines have similar coefficients and the colors and the textures have similar ones, too. In case of the sizes of apartment complexes, the colors and the textures of a large apartment complex (in the foreground) have similar coefficients, in case of that in the middle ground, the shapes and lines have similar coefficients. Fifth, as the results from estimating the contrasting properties of the landscape factor on the floors within the allowed scope of the landscape sensitivity, it was found that the contrasting property was 3.5 to 4.9 in case of a small scale of apartment complex (in the foreground), but 2.5to 3.7 in case of a small scale of one. In case of a large scale of apartment complex, the value was 3.5 to 5.3, but in case of a large one in the middle ground was 2.9 to 4.9. Sixth, it was comprehended that the contrasting properties of the landscape factor become different depending on each size of apartment complex and the distance of a view point. In this study, it is intended to find the meaning from the aspect that the results can be used as the baseline data for comprehending a proper range of heights of structures objectively during a natural landscape deliberation or a landscape deliberation.

Evaluation of Image Quality for Various Electronic Portal Imaging Devices in Radiation Therapy (방사선치료의 다양한 EPID 영상 질평가)

  • Son, Soon-Yong;Choi, Kwan-Woo;Kim, Jung-Min;Jeong, Hoi-Woun;Kwon, Kyung-Tae;Cho, Jeong-Hee;Lee, Jea-Hee;Jung, Jae-Yong;Kim, Ki-Won;Lee, Young-Ah;Son, Jin-Hyun;Min, Jung-Whan
    • Journal of radiological science and technology
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    • v.38 no.4
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    • pp.451-461
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    • 2015
  • In megavoltage (MV) radiotherapy, delivering the dose to the target volume is important while protecting the surrounding normal tissue. The purpose of this study was to evaluate the modulation transfer function (MTF), the noise power spectrum (NPS), and the detective quantum efficiency (DQE) using an edge block in megavoltage X-ray imaging (MVI). We used an edge block, which consists of tungsten with dimensions of 19 (thickness) ${\times}$ 10 (length) ${\times}$ 1 (width) $cm^3$ and measured the pre-sampling MTF at 6 MV energy. Various radiation therapy (RT) devices such as TrueBeam$^{TM}$ (Varian), BEAMVIEW$^{PLUS}$ (Siemens), iViewGT (Elekta) and Clinac$^{(R)}$iX (Varian) were used. As for MTF results, TrueBeam$^{TM}$(Varian) flattening filter free(FFF) showed the highest values of $0.46mm^{-1}$ and $1.40mm^{-1}$ for MTF 0.5 and 0.1. In NPS, iViewGT (Elekta) showed the lowest noise distribution. In DQE, iViewGT (Elekta) showed the best efficiency at a peak DQE and $1mm^{-1}DQE$ of 0.0026 and 0.00014, respectively. This study could be used not only for traditional QA imaging but also for quantitative MTF, NPS, and DQE measurement for development of an electronic portal imaging device (EPID).

Effects of Hairy Vetch and Rye Cover on Weed Occurrences and Minor Cereal Growth (피복작물처리에 따른 잡곡의 생육과 잡초발생)

  • Jeon, Seung-Ho;Lee, Se-Hun;Oh, Se-Yun;Kim, Young-Ju;Kim, Kyung-Moon;Kim, Seok-Hyun;Hwang, Jae-Bok;Yoon, Seong-Tak;Shim, Sang- In
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.56 no.2
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    • pp.134-139
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    • 2011
  • This study was conducted to evaluate the weed suppressing effects of two winter cover crops, hairy vetch and rye in foxtail millet and sorghum fields in 2010. Crop growth and development and weed occurrences in the fields were examined to know the efficiency of proposed cropping system. In hairy vetch treated plots, heading of minor cereals occurred early. The heading date was earlier by 1 day and 2 days in sorghum and foxtail millet, respectively. However, rye treatment delayed heading by 12 days and 8 days in sorghum and foxtail millet, respectively. Besides he effect of cover crop on ear emergence of crops, the Besides changed growth-related characteristics. Plant height, chlorophyll content and chlorophyll fluorescence of sorghum ere increased in hairy vetch treatment by 46.4% 88.7% and 7.9%, respectively. In foxtail millet, the characteristics ere also increased by 45.6%, 50.9% and 37.8%, respectively, s compared to control. Yields of sorghum and foxtail millet were increased by 105.1% and 135% as compared o control by hairy vetch treatment, respectively. However, he yields of cereal crops were decreased by rye cover crop treatment, the yields of sorghum and foxtail millet were decreased by 25.8% and 119.1%, respectively. Rye cover crop treatment inhibited crop growth suggesting nitrogen starvation in rye treated plots. In rye treatment, plant height, chlorophyll contents and chlorophyll fluorescence of sorghum ere slightly decreased by 7.1%, 10.8% and 10.8%, respectively, as compared to control whereas the inhibitory effects were greater in foxtail millet. Weed occurrences based n weed number in hairy vetch and rye plots were reduced n weed number in hairy vetch and rye plots were reduced y 27% and 20%, respectively. The smothering effect was weakened or disappeared after heading of crops. Weed number and dry weight in hairy vetch plot were increased by 159% and 55.2%, respectively, as compared to control. The results implied that weed suppressing of cover crops could be reduced drastically after heading of crops.

Short-term changes of phytoplankton communities after nutrient addition and establishment of stable mass culture condition to prepare the type approval test of USCG Phase-II in mesocosm enclosure (메소코즘에서 USCG phase-II 형식승인 대비 영양염 첨가에 따른 식물플랑크톤 대량 배양조건 확립 및 군집구조의 단주기변화)

  • Baek, Seung Ho;Lee, Min Ji;Shin, Kyoungsoon
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.4
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    • pp.34-42
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    • 2016
  • In order to prepare for the type approval test for the United States Coast Guard (USCG) Phase-II of Ballast Water Treatment System (BWTS), a phytoplankton mass culture was conducted in a mesocosm enclosure. We evaluated the response of the phytoplankton community after nutrient addition (+N, +P, and +NP) and investigated the development of the species with increasing culture time. After nutrient dosing, the phytoplankton population significantly (p < 0.05) increased from day 1 to day 3, depending on the nutrient treatments In particular, the specific growth rate of the phytoplankton community in the case of +NP treatment and + N treatment were estimated to be $2.47d^{-1}$ and $1.98d^{-1}$, respectively. The phytoplankton population density in the case of + NP treatment was approximately 50 times higher than that of the control group, suggesting that these treatments could be useful for mass culturing phytoplankton (> 75% of natural community) for the approval regulation of USCG Phase-II. In the phytoplankton community of the mesocosm, Pseudo-nitzchia spp. dominated in the logarithmic growth phase. The cell density decreased significantly (p < 0.05) with increasing time, coinciding with the nutrient limitation. At that time, the dominance of Pseudo-nitzchia spp. shifted to that of Cylindrotheca closterium. Therefore, the optimum nutrient concentration ($N:30{\mu}M$, $P:3{\mu}M$) and reasonable harvesting time (after 3 days in summer) found in this study for the mass culturing of phytoplankton may be helpful to meet the USCG Phase-II biological criteria to be used in BWTS.

Growth Characteristics and Economic Efficiency of Nursery Plants Production According to Transplanting Container for Acclimatization of Mass Propagated Plantlets via Bioreactor Culture of Ever-bearing Strawberry 'Goha' (생물반응기를 통해 대량증식된 사계성 딸기 '고하' 소식물체의 순화용기에 따른 생육특성과 묘생산 경제성)

  • Lee, Jong-Nam;Kim, Hye-Jin;Kim, Ki-Deog;Im, Ju-Sung;Lim, Hak-Tae;Yeoung, Young-Rok
    • Horticultural Science & Technology
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    • v.30 no.4
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    • pp.437-441
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    • 2012
  • This study was conducted to find out the optimum container for increasing acclimatization rate of in vitro mass propagated plantlets of Ever-bearing strawberry (Fragaria ${\times}$ ananassa Duch.) via bioreactor. Four types of containers were used such as transparent plastic container (TPC), plug tray (PT), I-pot (IP), and black vinyl pot (BVP). Number of date maintaining soil water content above 10% was five days in TPC, three to four days in BVP, two days in PT, and one day in IP. Survival rate of plantlets was 80% in BVP, 70% in TPC, 55% in IP, and 15% in PT. In TPC, growth increment of plantlets was the greatest among all the tested containers and the lowest in IP. Numbers of runner per plant were 3.3 in BVP, 2.9 in TPC, 1.6 in PT, and 1.2 in IP. Total cost was 44,405,300 won/10 a in BVP, resulting in reducing more 6,659,400 won/10 a than IP's (51,064,700 won/10 a). Around 102,718 plants/10 a were produced by using BVP, suggesting that 30,265.1 plants/10 a more could be produced than IP (72,452.9 plants/10 a). Production cost per plant was 432.3 won in BVP, resulting in reducing 272.5 won than IP's (704.8 won). As a result, BVP was appropriate for acclimatization of in vitro plantlets through bioreactor system.

Antioxidant capacity and Raw 264.7 macrophage anti-inflammatory effect of the Tenebrio Molitor (갈색거저리(Tenebrio Molitor)의 항산화능과 Raw 264.7 대식세포의 항염증 효과)

  • Yu, Jae-Myo;Jang, Jae-Yoon;Kim, Hyeon-Jeong;Cho, Yong-Hun;Kim, Dong-in;Kwon, O-jun;Cho, Yeong-Je;An, Bong-Jeun
    • Food Science and Preservation
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    • v.23 no.6
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    • pp.890-898
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    • 2016
  • The purpose of this paper is to investigate potential anti-inflammatory and anti-oxidant effects of Tenebrio molitor. Macrophage cell response by outside stimulation leads expression of pro-inflammatory cytokines, such as tumor necrosis $factor-{\alpha}$ ($TNF-{\alpha}$), interleukin-6 (IL-6), $interleukin-1{\beta}$ ($IL-1{\beta}$), and trigger expression of genes which are affected by inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), resulting in formation of inflammatory factors like nitric oxide (NO) and Prostaglandin $E_2$ (PGE2). Cell viability was determined by MTT assay. In order to investigate anti-inflammatory agents, the inhibitory effects on the production of lipopolysaccharide (LPS)-induced NO in RAW 264.7 cells were examined. T. Molitor significantly decreased the production of NO in a dose-dependent manner, and also reduced the expression of iNOS, a COX-2 protein. As a result, the levels of protein such as $PGE_2$, iNOS, COX-2 and MARKs were significantly reduced compared to non-treated group in T. Molitor water extract (TDW) treated group. Also, antioxidant effect of T. Molitor were investigated using DPPH, ABTS+ and superoxide anion radical scavenging activity tests in cell-free system. Antioxidant activity of T. molitor was found low in the DPPH radical scavenging test while high in the ABTS+ and superoxide anion radical scavenging activity tests. These results show that TDW could be an effective anti-pro-inflammatory and anti-oxidant agent.

Development of Energy Saving Aeration Panel for Aerating in Activated Sludge System (활성 슬러지조 폭기를 위한 에너지 절감형 판형 멤브레인 산기장치의 개발)

  • Kim, Ji Tae;Tak, Hyon Ki;Kim, Jong Kuk
    • Journal of Korean Society of Environmental Engineers
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    • v.34 no.6
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    • pp.414-420
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    • 2012
  • In an effort to commercialization of energy saving aeration apparatus, panel-type aeration membranes were prepared from polyurethane sheet of J company in Korea having tensile strength higher than $400kg_f/cm^2$ with thickness of 0.5mm. Micropores of 100 m size were made by poring technique utilizing needles. From lab-tests in 450 L water tank at temperature of $20^{\circ}C$, the performance of aeration panels at 40 L/min aeration rate showed 5 mg/L DO in less than 3 minutes approaching saturation point of 8 mg/L within 8 minutes. The results show very high efficiency with $K_{La(15)}$ ($16.34hr^{-1}$), Standard oxygen transfer efficiency (SOTE 54.7%) and Standard aeration efficienct (SAE 7.88 kg/kwh). Other pilot scale test in a $2m^3$ water tank with water temperature ($19^{\circ}C$) and aeration rate (30 L/min) showed DO exceeding 5 mg/L within 8 minutes along with $K_{La(15)}$ ($5.8hr^{-1}$), SOTE (42.1%) and SAE (6.41 kg/kwh). These efficiencies represent 2~2.5 times higher than conventional aeration devices. Especially, the achievement of higher Oxygen Transfer Rate indicate higher commercial viability. Conventional aeration devices when applied to clean water and wastewater frequently cause problems due to differences in actual Oxygen Transfer Rate. Our actual tests with $40^{\circ}C$ animal farm wastewater resulted very high efficiencies with Oxygen transfer efficiency ($OTE_f$ 22.1%) and $OTE_{pw40}$ (39.6%).

Optimization of Human Thrombopoietin Production in Insert Cells Using Baculovirus Expression System (베큘로 바이러스 발현 시스템에 의한 곤충세포에서의 인간 트롬보포이에틴 생산 최적화)

  • 고여욱;손미영;박상규;안혜경;박승국;박명환;양재명
    • KSBB Journal
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    • v.13 no.2
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    • pp.181-186
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    • 1998
  • In order to obtain high-level production of recombinant human thrombopoietin (rhTPO) in insect cell line, HTI-TN-5B1-4 (TN5), conditions for optimal rhTPO expression such as multiplicity of infection (MOI), the cell density at infection, harvesting time and type of culture method as well as growth media were determined. When TN5 cells were cultured as anchorage-dependent state in 60-mm dish, cell density $2\times^6$ cells,MOI of 10 and Garvesting the culture media at 72 hr post-infection wrere the cinditions for highest rh TPO production. High production of rhTPO was also achieved by using EXPRESS FIVE serum free media rather than SF900II serum free media-1. Anchorage-dependent TN5 cells were adapted as a suspension culture when they were grown in the presence of heparin. TN5 cells were successfully cultured at 0.2 L scale in suspension culture without having aggregation. When TN5 cells were cultured as suspension state, cell density of $0.6\times10^6$ cells/mL, MOI of 1 and harvesting the culture media at 72 hr post-infection, gave the highest yield of rhTPO.

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