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Monitoring Bacillus cereus and Aerobic Bacteria in Raw Infant Formula and Microbial Quality Control during Manufacturing  

Jung, Woo-Young (Hazardous Substances Analysis Division, Daejeon Regional Food and Drug Administration)
Eom, Joon-Ho (Hazardous Substances Analysis Division, Daejeon Regional Food and Drug Administration)
Kim, Byeong-Jo (Hazardous Substances Analysis Division, Daejeon Regional Food and Drug Administration)
Ju, In-Sun (Hazardous Substances Analysis Division, Daejeon Regional Food and Drug Administration)
Kim, Chang-Soo (Hazardous Substances Analysis Division, Daejeon Regional Food and Drug Administration)
Kim, Mi-Ra (Hazardous Substances Analysis Division, Daejeon Regional Food and Drug Administration)
Byun, Jung-A (Hazardous Substances Analysis Division, Daejeon Regional Food and Drug Administration)
Park, You-Gyoung (Hazardous Substances Analysis Division, Daejeon Regional Food and Drug Administration)
Son, Sang-Hyuck (Hazardous Substances Analysis Division, Daejeon Regional Food and Drug Administration)
Lee, Eun-Mi (Hazardous Substances Analysis Division, Daejeon Regional Food and Drug Administration)
Jung, Rae-Seok (Hazardous Substances Analysis Division, Daejeon Regional Food and Drug Administration)
Na, Mi-Ae (Hazardous Substances Analysis Division, Daejeon Regional Food and Drug Administration)
Yuk, Dong-Yeon (Hazardous Substances Analysis Division, Daejeon Regional Food and Drug Administration)
Gang, Ji-Yeon (Hazardous Substances Analysis Division, Daejeon Regional Food and Drug Administration)
Heo, Ok-Sun (Hazardous Substances Analysis Division, Daejeon Regional Food and Drug Administration)
Yoon, Min-Ho (Department of Bio-Environmental Chemistry, College of Agriculture and Life Sciences, Chungnam National University)
Publication Information
Korean Journal of Food Science and Technology / v.42, no.4, 2010 , pp. 494-501 More about this Journal
Abstract
The purpose of this study was to examine the presence of Bacillus cereus, aerobic bacteria and coliforms in the raw material of infant formulas and investigate the manufacturing process in terms of microbial safety. Among ten kinds of raw infant formula material samples (n=20), Bacillus cereus appeared in two (n=4). Aerobic bacteria were not detected in raw infant formula material or maximum 4.15 log CFU/g. Eleven species of aerobic bacteria were isolated and 76% of them were Sphingomonas paucimobilis, Pseudomonas fluorescens, Rhizobium radiobactor, or Stenotrophomonas maltophilia. A Pearson's correlation analysis revealed that the most influential factors for detecting Bacillus cereus were aerobic bacteria and coliforms. In other words, when the measured values of aerobic bacteria and coliforms were higher, the possibility that Bacillus cereus would appear increased. In a regression model to predict Bacillus cereus, the rate of appearance was correlated with aerobic bacteria and coliforms, and its contribution rate for effectiveness was 86%. Improving microbial quality control by pasteurization, spray dry, popping and extrusion resulted in a decrease in the numbers of Bacillus cereus, aerobic bacteria and coliforms in the raw materials. The results suggest that a hazard analysis and critical control point system might be effective for reducing microbiological contamination.
Keywords
Bacillus cereus; aerobic bacteria; infant formulas; coliforms; microbial quality control;
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1 Gillian AF, Christopher T, David O. The microbiological safety of minimally processed vegetables. Int. J. Food Sci. Technol. 34: 1-22 (1999)   DOI   ScienceOn
2 Park SI. A study on the sensory characteristics of virus heat treated milk. J. Fd. Hyg. Safety. 10: 19-22 (1995)
3 Lee SC, Kim EM, Chang KS. Optimization of fluidized bed agglomeration in the production of weaning food. Food Eng. Progress. 11: 89-95 (2007)
4 Whitney R Mcl. 3rd. Proteins of Milk. Fundamentals of Dairy Chemistry, Van Nostrand-Reinhold, New York, NY, USA, pp. 81-169 (1988)
5 Brown RJ. 3rd. Milk Coagulation and Protein Denaturation. Fundamentals of Dairy Chemistry, Van Nostrand-Reinhold, New York, NY, USA, p. 583 (1988)
6 Jeon JW, Yun SS, Juhn SL, Chun HN. The current quality control and state of scorched particles in infant formula in Korea. Korean J. Dairy Sci. Technol. 25: 55-60 (2006)
7 Keum JS. Pathogenic bacterias in milk and their characteristics. Korean Dairy Technol. 16: 35-43 (1998)
8 Kim JH, Lee SY, Kim KBWR, Song EJ, Kim AR, Park SM, Han CS, Ahn DH. Antimicrobial activity of electrolyzed alkaline water against spoilage of microorganisms in rice warehouses. J. Korean Soc. Food Sci. Nutr. 36: 111-116 (2007)   과학기술학회마을   DOI
9 Choi SH, Lee SB, Choi JW. Microbiological Safety of infant formula and baby food. J. Korean Dairy Technol. Sci. 23: 65-71 (2005)
10 Vagn Westergaard. 5th. Milk Powder Technology Evaporation and Spray Drying. K. Masters, Niro A/S, Copenhagen, Denmark. pp. 1-337 (2004)
11 Jeong SG, Ham JS, Kim DH, Ahn CN, Chae HS, You YM, Kwon IK, Lee SG. Research on the quality characteristics of domestic colostrum according to the processing methods employed. Korean J. Food Sci. Anim. Resour. 29: 457-465 (2009)   과학기술학회마을   DOI
12 Bryan FL. Epidemiology of milk-borne diseases. J. Food Protect. 46: 637 (1983)
13 Muytjens HL, van der Roelofs-Willemse H, Jasper GHL. Quality of powdered substitutes for breast milk with regard to members of the family Enterobacteriaceae. J. Clin. Microbiol. 26: 743-746 (1988)
14 Park SY, Park JM, Yang JO, Jung HK, Chun HN, Lee BH. Microbial qualities and post-pasteurization contaminations of UHT milk produced in Korea. J. Korean Dairy Technol. Sci. 24: 9-18 (2006)
15 Reyes JE, Bastias JB, Gutierrez MR, Rodriguez MO. Prevalence of Bacillus cereus in dried milk products used by children school feeding program. Food Microbiol. 24: 1-6 (2007)   DOI   ScienceOn
16 Hsieh YM, Sheu SJ, Chen YL, Tsen HY. Enterotoxigenic profiles and polymerase chain reaction detection of Bacillus cereus group cells and B.cereus strains from foods and food-borne outbreaks. J. Applied microbiol. 87: 481-490 (1999)   DOI   ScienceOn
17 Wijnand LM, Dufrenne JB, Rombouts FM, PH in Veld, Leusden FM van. Prevalence of potentially pathogenic Bacillus cereus in food commodities in the netherlands. J. Food Protect. 69: 2587-2594 (2006)
18 Park HW, Federici BA. Genetic factors affecting insecticidal crystal protein synthesis in Bacillus thuringiensis. Int. J. Indus. Entomol. 1: 1-7 (2000)
19 Choi SY, Cho MS, Kim TH, Kim JS, Park SK, Youn YN, Hong SS, Yu YM. Bioactive characterization of Bacillus thuringiensis subsp. kurstaki CAB133 isolated from domestic soil. Korean J. Appl. Entomol. 47: 175-184 (2008)   과학기술학회마을   DOI
20 Duc Le H, Dong TC, Lagan NA, Sutherland AD, J.Taylor SM. Cutting, cases of emesis associated with bacterial contamination of an infant breakfast cereal product. Int. J. Food Microbiol. 102: 245-251 (2005)   DOI   ScienceOn
21 Rowan NJ, Anderson JG. Diarrhoeal enterotoxin production by psychrotrophic Bacillus cereus present in reconstitued milk-based infant formulae(MIF). Lett. Appl. Microbiol. 26: 161-165 (1998)   DOI   ScienceOn
22 Sarrias JA, Valero M, Salmeron MC. Enumeration, isolation and characterization of Bacillus cereus strains from spanish raw rice. Food Microbiol. 19: 589-595 (2002)   DOI   ScienceOn
23 Choi BK, Park SY, Ha SD, Kum JS, Lee HY, Park JD. Effect of drying methods of rice flour on growth properties. Korean J. Food Sci. Technol. 39: 295-298 (2007)   과학기술학회마을
24 Yeun CH, Song YB, Yoon CY, Jung WY, Cho SY, Kim MG, Song YM, Lee JH, Kang CS, Kuk JH, Bae MS, Kim JH, Joo IS, Kwak IS, Lim CJ, Lee KH, Kim OH. Prevalence of Bacillus cereus in foods for infants and young children. The Annual Report of KFDA, Korea. 11: 70-92 (2007)
25 Kim SJ, Jung JH, Tahk HM, Baek SY, Lee SY. Effect of factors on the sporulation of Bacillus cereus and their thermal resistance. J. Fd. Hyg. Safety 24: 256-261 (2009)   과학기술학회마을
26 Ankolekar C, Rahmati T, Labbe RG. Detection of toxigenic Bacillus cereus and Bacillus thuringiensis spore in U.S. rice. Int. J. Food Microbiol. 128: 460-466 (2008)
27 Kramer JM, Gilbert RJ. Bacillus cereus and other Bacillus species. In Foodborne Bacterial Pathogens. Doyle MP (ed). Marcel Dekker Inc, London, UK, pp. 21-70 (1989)
28 Monica ES, Fricker M, Scherer S. Bacillus cereus the causative agent of an emetic type of food-borne illness. Mol. Nutr. Food Res. 48: 479-487 (2004)   DOI   ScienceOn
29 Hwang JY, Lee JY, Park JH. Microbiological quality and potential pathogen monitering for powdered formulas from the local market. Korean J. Food Sci. Anim. Resour. 28: 555-561 (2008)   과학기술학회마을   DOI
30 Cho YS, Jung EY, Lee MK, Yang CY, Shin DB. Survival, isolation, and characterization of Bacillus cereus from sunshik. J. Fd. Hyg. Safety. 23: 343-347 (2008)   과학기술학회마을
31 Lee MS, Chang DS. Growth rate of Bacillus cereus and heat resistance of its spores. Korean J. Food Sci. Technol. 14: 11-15 (1982)
32 Woon JH, Lee JY, Lim KJ. Strengthening measures of the hygiene control for infant formula based on milk. Safe Food 2: 5-19 (2007)
33 Shin HW, Lim YH, Lee JK, Kim YJ, Oh SW, Shin CS. Effect of commercial antimicrobial in combination with heat treatment on inactivation of Bacillus cereus spore. Food Sci. Biotechnol. 17: 603-607 (2008)   과학기술학회마을
34 Bae KW, Kim JH, Kim YS, Park JS, Jee YK, Lee KY. Microbiological identification and distribution of metal components in suspended particulate matter during yellow sand phenomena at Taean region in 2003. Tuberculosis Resp. Dis. 58: 167-173 (2005)