• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.11a
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• pp.408-408
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• 2005

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 1996.05a
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• pp.41-62
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• 1996

Since 1966 tissue culture has been used as a tool for the production of disease indexed stocks from selected plants and their rapid (clonal) mass propagation through the procedure now referred to as micropropagation. The major advantages have been the rapid introduction of new plant cultivars, created within conventional and mutation breeding programmes, as healthy stock for beneficial distribution and the expansion of the world wide horticultural industry. (omitted)

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 1996.05a
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• pp.63-73
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• 1996

The importance of plant factory which blend agriculture and manufacture has been discussed widely. This company has researched hydroponics technique for cultivation which has been mainly developed in the sites of Okinawa and Hyougo Prefecture. In 1987, we stopped the previous research there, and started a new one far plant factory at the Agricultural Research Center of Chiba Prefecture. In 1989, we built four experimental plant factories (120㎡) at Chiba Oil Factory Refinery in Ichihara-city, developing techniques of cultivation, equipment production and environmental control for cultivation system of tomato, strawberry and leaf vegetables. (omitted)

• Korean Journal of Agricultural Science
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• v.43 no.4
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• pp.522-536
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• 2016

Fluorine is unique chemical element which occurs naturally, but is not an essential nutrient for plants. Fluoride toxicity can arise due to excessive fluoride intake from a variety of natural or manmade sources. Fluoride is phytotoxic to most plants. Plants which are sensitive for fluorine exposure even low concentrations of fluorine can cause leave damage and a decline in growth. All vegetation contains some fluoride absorbed from soil and water. The highest levels of F in field-grown vegetables are found up to $40mg\;kg^{-1}$ fresh weight although fluoride is relatively immobile and is not easily leached in soil because most of the fluoride was not readily soluble or exchangeable. Also, high concentrations of fluoride primarily associated with the soil colloid or clay fraction can increase fluoride levels in soil solution, increasing uptake via the plant root. In soils more than 90 percent of the natural fluoride ranging from 20 to $1,000{\mu}g\;g^{-1}$ is insoluble, or tightly bound to soil particles. The excess accumulation of fluorides in vegetation leads to visible leaf injury, damage to fruits, changes in the yield. The amount of fluoride taken up by plants depending on the type of plant, the nature of the soil, and the amount and form of fluoride in the soil should be controlled. Conclusively, fluoride is possible and long-term pollution effects on plant growth through accumulation of the fluoride retained in the soil.

• The Plant Pathology Journal
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• v.38 no.6
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• pp.593-602
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• 2022

Anthracnose, caused by the ascomycete fungus Colletotrichum scovillei, is a destructive disease in pepper. The fungus germinates and develops an infection structure called an appressorium on the plant surface. Several signaling cascades, including cAMP-mediated signaling and mitogen-activated protein kinase (MAPK) cascades, are involved in fungal development and pathogenicity in plant pathogenic fungi, but this has not been well studied in the fruit-infecting fungus C. scovillei. Ste50 is an adaptor protein interacting with multiple upstream components to activate the MAPK cascades. Here, we characterized the CsSTE50 gene of C. scovillei, a homolog of Magnaporthe oryzae MST50 that functions in MAPK cascades, by gene knockout. The knockout mutant ΔCsste50 had pleiotropic phenotypes in development and pathogenicity. Compared with the wild-type, the mutants grew faster and produced more conidia on regular agar but were more sensitive to osmotic stress. On artificial and plant surfaces, the conidia of the mutant showed significantly reduced germination and failed to form appressoria. The mutant was completely non-pathogenic on pepper fruits with or without wounds, indicating that pre-penetration and invasive growth were both defective in the mutant. Our results show that the adaptor protein CsSTE50 plays a role in vegetative growth, conidiation, germination, appressorium formation, and pathogenicity in C. scovillei.

• Korean Journal of Plant Taxonomy
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• v.52 no.2
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• pp.123-126
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• 2022

Hibiscus sabdariffa L., (roselle) in the Malvaceae family is an erect subshrub known to be native to India and Malaysia. It is widely used as a food or tea material around the world, and its therapeutic effects have been widely studied. In this study, the sequencing of the complete chloroplast genome of H. sabdariffa was carried out. The result indicates a genome size of 162,428 bp, which is composed of a large single copy of 90,327 bp, two inverted repeats of 26,242 bp each, and a small single copy of 19,617 bp. Overall, a total of 131 genes were predicted, including 86 coding sequences, 37 tRNAs, and 8 rRNAs. According to a phylogenic analysis, it was clearly distinguished from outgroups such as other species of the genus Hibiscus used in the analysis.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.1
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• pp.66-73
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• 2012

Plant growth promoting traits like production of indoleacetic acid (IAA), ammonia, hydrogen cyanide (HCN), siderophore, and like the enzyme activities of catalase, ACC deaminase, cellulase, chitinase and protease were assayed in vitro for twenty one phosphorus solubilizing bacteria isolated from soil isolates. Except SPP-5 and SPP-15 strains, all the other isolated strains produced IAA in various amounts of 10 to $23{\mu}g\;ml^{-1}$. All strains showed positive response for ammonia production and ACC deaminase activity implying that they are capable of growing in a N-free basal medium. Catalase activity was found to be superior in SPP-2, SPP-7, SPP-12 and SPP-17 compared to the other strains tested. HCN production was detected by 15 strains and among them SPP-9, SPP-15, SAph-11, and SAph-24 were found to be strong HCN producers. Except the isolates SPP-10, SPP-12, SPP-13 and SPP-14, all the other isolates produced more than 80% siderophore units. None of the strains showed cellulose and chitinase activity. SAph-8, SAPh-11, SAPh-24 and SPP-15 strains showed 35.84, 50.33, 56.64 and 34.78 U/ml protease activities, respectively. SPP-1, SPP-2, SPP-3, SPP-11, SPP-17, SPP-18, SAph-11 and SAph-24 strains showed positive response for all the tested plant growth promotion traits except cell wall degrading enzyme activities. According to the results, all the tested phosphorus solubilizing isolates could exhibit more than three or four plant growth promoting traits, which may promote plant growth directly or indirectly or synergistically. Therefore, these phosphorus solubilizing strains could be employed as bio-inoculants for agriculture soils.

• The Plant Pathology Journal
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• v.37 no.6
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• pp.607-618
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• 2021

The pepper anthracnose fungus, Colletotrichum scovillei, causes severe losses of pepper fruit production in the tropical and temperate zones. RAC1 is a highly conserved small GTP-binding protein in the Rho GT-Pase family. This protein has been demonstrated to play a role in fungal development, and pathogenicity in several plant pathogenic fungi. However, the functional roles of RAC1 are not characterized in C. scovillei causing anthracnose on pepper fruits. Here, we generated a deletion mutant (𝜟Csrac1) via homologous recombination to investigate the functional roles of CsRAC1. The 𝜟Csrac1 showed pleiotropic defects in fungal growth and developments, including vegetative growth, conidiogenesis, conidial germination and appressorium formation, compared to wild-type. Although 𝜟Csrac1 was able to develop appressoria, it failed to differentiate appressorium pegs. However, 𝜟Csrac1 still caused anthracnose disease with significantly reduced rate on wounded pepper fruits. Further analyses revealed that 𝜟Csrac1 was defective in tolerance to oxidative stress and suppression of host-defense genes. Taken together, our results suggest that CsRAC1 plays essential roles in fungal development and pathogenicity in C. scovilleipepper fruit pathosystem.

• Journal of the Korean Society of Combustion
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• v.19 no.2
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• pp.28-36
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• 2014

Bio fuel oil combustion experiments were successfully demonstrated at the 75 MWe oil-fired power plant without major equipment retrofit and 100% bio-fuel oil combustion was possible without big problems. The experimental data error correction was conducted and numerical model-based analysis technique was applied for the evaluation of the results. Incase of bio fuel oil combustion, heat absorption of radiative heat transfer section was reduced while convection section has opposite trend. The furnace exit gas temperature tends to rise slightly. Environment emissions such as NOx and SOx concentrations showed a tendency to decrease during the bio fuel oil combustion period. On the other hand, boiler efficiency was slightly underestimated.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2000.11c
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• pp.770-776
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• 2000

This paper represents our efforts to diagnose nutrient stresses using physiological instruments in cucumber plants. The stresses could be detected by measuring and analyzing the difference of chlorophyll content, photosynthetic efficiency(Fv/Fm), differential temperature(DT), stomatal resistance and light absorbance values between deficient and controlled plants. From the all over experiments, the stresses could be first diagnosed in the 8th day after treatment and the overall diagnosis rate was estimated at more than 50%.