• 제목/요약/키워드: Binding Capacity

검색결과 681건 처리시간 0.028초

A Possible Merge of FRET and SPR Sensing System for Highly Accurate and Selective Immunosensing

  • Lee, Jae-Beom;Chen, Hongxia;Lee, Jae-Wook;Sun, Fangfang;Kim, Cheol-Min;Chang, Chul-Hun L.;Koh, Kwang-Nak
    • Bulletin of the Korean Chemical Society
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    • 제30권12호
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    • pp.2905-2908
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    • 2009
  • Immuno-sensing for high accurate and selective sensing was performed by fluorescence spectroscopy and surface plasmon resonance (SPR), respectively. Engineered assembly of two fluorescent quantum dots (QDs) with bovine serum albumin (BSA) and anti-BSA was fabricated in PBS buffer for fluorescence analysis of fluorescence resonance energy transfer (FRET). Furthermore, the same bio-moieties were immobilized on Au plates for SPR analysis. Naturally-driven binding affinity of immuno-moieties induced FRET and plasmon resonance angle shift in the nanoscale sensing system. Interestingly, the sensing ranges were uniquely different in two systems: e.g., SPR spectroscopy was suitable for highly accurate analysis to measure in the range of 10$^{-15{\sim}-10$ng/mL while the QD fluorescent sensing system was relatively lower sensing ranges in 10$^{-10{\sim}-6$ng/mL. However, the QD sensing system was larger than the SPR sensing system in terms of sensing capacity per one specimen. It is, therefore, suggested that a mutual assistance of FRET and SPR combined sensing system would be a potentially promising candidate for high accuracy and reliable in situ sensing system of immune-related diseases.

쾌속조형기에 의한 기어식 주유장치의 개발 (Development of gear type grease lubricator by rapid prototyping)

  • 왕덕현
    • 한국기계가공학회지
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    • 제13권6호
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    • pp.46-53
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    • 2014
  • An automatic grease lubricator provides an adequate amount of fresh grease constantly to any type of rotating machine to minimize friction heat and reduce friction loss. This study seeks to develop an automatic grease lubricator by means of rapid prototyping with a gear-driven mechanism and a controlled operation time. The ultimate design is to lubricate an adequate amount of grease by a simple dip-switch clicking mechanism according to an advanced set cycle. The backlash of the gear was minimized to increase the power, and to increase the power of the mechanism, the binding frequency and the thickness of the coil were changed. To control the rotation cycles of the main shaft according to certain set numbers, different resistances and chips were used in the design of the circuit which controls the electrical signals via a pulse. A digital mock-up was analyzed and the rapid prototyping (RP) trial products were tested with a PCB circuit and grease. An evaluation of the outlet capacity of RP trial products was conducted, as the friction caused by the outlet on the wall surface was an important factor in the operation of the equipment. Finally, a finishing process was applied to decrease the roughness of a surface to a comparable level to test the performance of the product.

수침과 수세가 도토리 묵가루의 이화학적 특성에 미치는 영향 (Effects of Steeping and Washing on Physicochemical Properties of Acorn Flour)

  • 나환식;박종훈;김관
    • 한국식품저장유통학회지
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    • 제5권4호
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    • pp.368-373
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    • 1998
  • 도토리 묵 제조의 최적조건을 찾기 위한 기초자료를 얻기 위하여 수침과 수세정도를 달리하여 제조한 도토리 묵가루의 일반성분 및 이화학적 특성을 조사하였다. 수침과 수세의 정도가 증가할수록 조단백질, 회분, 총페놀은 감소하였으며, 조지방과 식이섬유소는 변화가 거의 없었다. 색도 또한 L값은 증가하였고, a값과 b값은 감소하는 경향을 보였다. 팽윤력은 수침과 수세정도가 증가할수록 증가하였으며, 용해도는 처리정도에 따라 감소하였으나 변화양상이 일정하지는 않았다. 물결합능력은 대조구에서 높았으며 처리정도에 따라 감소하였다. 고유점도 또한 처리정도에 따라 증가하였다. 이러한 결과들은 수침보다는 수세에 의한 영향이 더 컸다.

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생강 전분과 옥수수 가교 전분의 이화학적 성질 비교 (Comparison of Some Physicochemical Properties of Ginger Root and Cross-linked Corn Starches)

  • 허준;김성곤
    • 한국식품과학회지
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    • 제16권2호
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    • pp.201-205
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    • 1984
  • 생강전분의 몇 가지 물리화학적 성질을 옥수수 가교 전분과 비교 검토하였다. 생강전분은 아밀로스 함량이 23.5%, 물결합 능력이 99.1%이었고 B형의 X-ray 회절도를 보였다. 생강 전분의 광투과도는 $70^{\circ}C$ 이후에 증가하였고 옥수수 가교 전분의 swelling power와 용해도는 $90^{\circ}C$까지 아주 낮은 값을 보였으며, 알카리에 대하여도 안정하였다. 아밀로그라피에 의한 생강전분 (7%)의 초기 호화온도는 $81.5^{\circ}C$ 이었고, 옥수수 가교전분과 같이 안정한 Paste를 보였다.

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수분 열처리한 감자 및 고구마전분의 호화 특성 (Gelatinization Properties of Heat-Moisture Treated Potato and Sweetpotato Starches)

  • 김성곤;이신영;박용곤
    • 한국식품과학회지
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    • 제19권5호
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    • pp.435-440
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    • 1987
  • 감자 및 고구마 전분의 수분-열처리에 따른 호화특성을 비교하였다. 수분-열처리에 의하여 전분의 물결합능력은 증가하였으며 그 정도는 고구마전분이 더 현저하였다. 전분의 blue value는 수분-열처리에 의하여 영향을 받지 않았다. 아밀로그라프의 점도는 수분-열처리에 의하여 크게 감소하였으며, 최고 점도는 나타나지 않았다. 가성소다에 의한 전분의 호화는 감자가 고구마 전분보다 쉬웠으며, 수분-열처리에 의하여 호화에 필요한 알칼리의 농도는 증가하였다. KSCN에 의한 전분겔의 부피는 감자가 고구마전분보다 높았다. 감자 및 고구마 호화전분은 항복응력을 갖는 의가소성 유체의 성질을 보였으며, 수분-열처리에 의하여 점조도지수 및 항복응력이 크게 감소하였다.

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건열처리가 옥수수 전분의 성질에 미치는 영향 (Effect of dry-heat treatment on properties of corn starch)

  • 서정식;이만정;김성곤
    • Applied Biological Chemistry
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    • 제35권5호
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    • pp.389-394
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    • 1992
  • 건열$(130{\sim}220^{\circ}C)$ 처리가 옥수수 전분의 성질에 미치는 영향을 조사하였다. 표면 색도의 경우 L값과 a값은 큰 차이가 없었으나 b값은 열처리에 따라 약간 감소하였다. Blue value는 $190^{\circ}C$ 이상에서 약간 증가하였고 물결합능력은 열처리 온도의 상승에 따라 증가하였다. 팽윤력은 시료간에 큰 차이가 없으나 $90^{\circ}C$에서의 팽윤력은 $190^{\circ}C$ 이상의 시료에서 약간 감소하였고 용해도는 $190^{\circ}C$ 이상에서 증가하였다. $190^{\circ}C$ 이상에서 고유점도의 상당한 감소, 전분결정도의 감소, 산 또는 효소에 의한 가수분해율의 뚜렷한 증가경향을 나타내었다.

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품종별 한국산 감자의 전분 특성 비교(I) (Comparison of Physicochemical Properties of Several Korean Potato Starches)

  • 김경애;김선민;정난희
    • 한국식품조리과학회지
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    • 제5권1호
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    • pp.53-62
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    • 1989
  • 분질감자인 남작, 중간질감자인 세풍과 수미, 점질감자인 대지마로부터 전분을 분리하여 입자의 형태, 이화학적 성질 및 호화 특성을 비교 검토하였다. 전분입자는 모두 원형과 타원형이고 입자의 크기는 남작과 세풍 전분은 $11~30\mu\textrm{m}$ 수미와 대지마 전분은 $21~40 \mu\textrm{m}$의 입자가 주를 이루었다. Blue value, 아밀로오스 함량, 상대결정도, 밀도는 남작 전분이 가장 높았으며 대지마전분이 가장 낮았다. 물결합 능력, 팽윤력, 광투과도, 아밀로그라프에 의한 최고점도와 breakdown은 수미전분이 가장 높았으며 남작 전분이 가장 낮았다. 호화개시온도는 남작 전분이 가장 높았으며 수미 전분이 가장 낮았다. DSC에 의한 호화엔탈피는 품종간의 차이가 뚜렷하지 않았다.

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수침과 입자크기를 달리한 쌀가루와 쌀 만주제조 특성 (Characteristics of Preparation of Rice Manju and Rice Flour with Soaking and Different Particle Sizes)

  • 이승현;신말식
    • 한국식품조리과학회지
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    • 제25권4호
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    • pp.427-434
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    • 2009
  • To increase rice consumption and substitute rice flour for wheat flour to make gluten-free bakery products, the physicochemical and pasting properties of rice flours prepared from raw and soaked rices passed through different size screens were investigated. The quality properties of manju dough and preparation of rice manju were also measured. Dry milled flour with soaked rice (DMFSR) were decreased in ash and crude lipid contents compared to dry milled flour with raw rice (DMFRR). Water binding capacity, damaged starch content, and L value of rice flour increased with decreasing particle size, but the b value decreased. Peak, cold, and breakdown viscosities of DMFSR were higher than those of DMFRR by RVA. Hardness of manju dough with DMFSR was lower than that with DMFRR, but that of manju shell exhibited a reverse trend. Sensory difference testing revealed the smoothness of surface, hardness, roughness, and overall quality were significantly different (p<0.05). The smoothness of the surface of manju with DMFRR-200 and all DMFSR were better than that of manju prepared with wheat flour. Hardness showed lower values in DMFRR-200, and all DMFSR as well as wheat flour and roughness decreased with decreasing particle size. Rice manju with wheat flour, DMFRR-200, DMFSR-120, and DMFSR-170 ranked above 5 points and were not significantly different (p<0.05).

식이섬유 첨가가 절편의 특성에 미치는 영향에 관한 연구 (A Study on the Effect of Addition of Dietary Fibers on Quality of Julpyun)

  • 이지영;구성자
    • 한국식품조리과학회지
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    • 제10권3호
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    • pp.267-276
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    • 1994
  • Recent epidemological observations suggested beneficial effects of dietary fiber on man's health. With the increased emphasis placed on the requirement for dietary fiber in the diet, there has been greater interest in successfully incorporating dietary fiber into food products. Hence, in this study, several dietary fibers such as cellulose, pectin, rice bran, barley bran and job's tears bran were incorporated into julpyun to determine their effects on some physical properties and sensory characteristics. Difference of water binding capacity(WBC) was found among dietary fiber sources. The WBC of pectin was highest and that of job's tears bran was lowest. The moisture contents of julpyun were increased as WBC of added dietary fiber sources was higher. In the rheometer measurement for julpyun added with dietary fiber, hardness of julpyuns added with cellulose and pectin was low. But, hardness of julpyun added cellulose was increased rapidly during storage at 4$^{\circ}C$. Springiness and gumminess wereshowed similar aspects to hardness. In the retrogradation speed by Avrami equation, the rate constant of julpyun added barley bran was low ao retrogradation occurred slowly. The results of sensory evaluation, julpyun added no dietary fiber showed good overall preference. Overall preference in sensory evaluation showed positive correlation with the after-swallowing and color.

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Immobilization of potassium copper hexacyanoferrate in doubly crosslinked magnetic polymer bead for highly effective Cs+ removal and facile recovery

  • Kim, Yun Kon;Bae, Kyeonghui;Kim, Yonghwan;Harbottle, David;Lee, Jae W.
    • Journal of Industrial and Engineering Chemistry
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    • 제68권
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    • pp.48-56
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    • 2018
  • A potassium copper hexacyanoferrate (KCuHCF) embedded magnetic hydrogel bead (HCF-Mbead) was synthesized via a facile double crosslinking methods of $Fe^{3+}$ ionic binding and freeze-thaw for effective $Cs^+$ removal. The HCF-Mbead had a hierarchical porous structure facilitating fast access of $Cs^+$ ions to embedded active sites. The adsorbent showed enhanced $Cs^+$ removal properties in terms of capacity (69.2 mg/g), selectivity ($K_d=4{\times}10^4mL/g$, 1 ppm $Cs^+$ in seawater) and stability (>99.5% removal in pH 3~11) with rapid magnetic separation. This study further opens the possibility to develop an efficient material that links the integration of adsorption and recovery.