• ALGAE
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• v.29 no.4
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• pp.343-353
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• 2014

Despite the extensive literature on marine algae over the past few decades, a paucity of published research and studies exists on red algae. The purpose of this study was to evaluate the potential therapeutic properties of the ethanol extract of the red alga Callophyllis japonica against lipopolysaccharide (LPS)-stimulated macrophage inflammation. The C. japonica extract (CJE) significantly inhibited the nitric oxide (NO) production and the induced dose-dependent reduction of the protein and mRNA levels of inducible nitric oxide synthase and cyclooxygenase-2. Additionally, the CJE reduced the mRNA levels of inflammatory cytokines, including tumor necrosis factor-${\alpha}$, interleukin (IL)-$1{\beta}$, and IL-6. We investigated the mechanism by which the CJE inhibits NO by examining the level of mitogen-activated protein kinases (MAPKs) activation, which is an inflammation-induced signaling pathway in macrophages. The CJE significantly suppressed the LPS-induced phosphorylation of c-Jun N-terminal kinase, extracellular signal-regulated kinase and p38 MAPK. Taken together, the results of this study demonstrate that the CJE inhibits LPS-induced inflammation by blocking the MAPK pathway in macrophages.

• Journal of Convergence for Information Technology
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• v.11 no.8
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• pp.92-99
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• 2021

Light-scattering fine particulate matter monitors can measure particulate matter (PM) concentrations in every second and can be designed in a portable size. They can measure the concentrations of various PM sizes (PM_1.0, PM_2.5, PM_4.0 and PM₁₀) with a single sensor. They measure the number and size of particulate matters and convert them to weight per volume (concentration). These devices show a large error for asian dust. This paper proposes a scheme that compensates the PM_2.5 concenstration error for asian dust by multiple linear regression machine learning in light-scattering PM monitors. This scheme can be effective with only two or three types of PM sizes. The experimental results compare a beta-ray PM monitor of national institute of environmental research and a light-scattering PM monitor during a month. The correlation coefficient (R²) of theses two devices was 0.927 without asian dust, but it was 0.763 due to asian dust during the entire experimental period and improved to 0.944 by the proposed machine learning.

• Journal of Life Science
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• v.27 no.2
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• pp.187-193
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• 2017

As a part of ongoing research to elucidate and characterize antiinflammatory nutraceuticals, the crude extracts from Atriplex gmelinii C. A. Mey. and their solvent-partitioned fractions were tested for their antiinflammatory potential in lipopolysaccharide (LPS)-stimulated RAW 264.7 mouse macrophages. The crude extracts of A. gmelinii C. A. Mey. were fractioned according to polarity with n-hexane, 85% aqueous methanol (85% aq. MeOH), n-butanol, and $H_2O$. Their antiinflammatory activities were investigated in LPS-induced inflammation in mouse macrophages by measuring nitric oxide (NO) generation and mRNA expression of inflammation mediators, namely, inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin-$1{\beta}$ ($IL-1{\beta}$), and IL-6. As a result, we confirmed that the crude extracts of A. gmelinii C. A. Mey. inhibited LPS-stimulated NO production and mRNA expression of iNOS and COX-2 as important inflammatory factors. The inhibition of NO production through the downregulation of important inflammatory factors such as iNOS, COX-2, $IL-1{\beta}$, and IL-6 was found by treatment with all solvent-partitioned fractions. Among all tested fractions, 85% aq. MeOH showed the strongest antiinflammatory response. Based on the current results, A. gmelinii C. A. Mey. was suggested to possess natural antiinflammatory components, indicating that it could be used as a valuable source of antiinflammatory substances.

• Journal of Life Science
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• v.27 no.4
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• pp.456-463
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• 2017

The purpose of this study was to investigate the effect of 3 types of medicinal herbs (Glycyrrhizae radix, Astragali radix and Dioscorea rhizoma) extracts on estrogen-like activities, proliferation and differentiation in osteoblast. Human breast cancer cell line MCF7 was transfected using an estrogen responsive luciferase reporter plasmid for measure the estrogen-like activity. Estrogen-like activities of extracts were in the range of 1.11~5.73 fold to that of negative control. The extract of G. radix showed the strongest estrogen-like activities. The estrogen-like activities of 50 and $500{\mu}g/ml$ extracts of G. radix were similar to that of $10^{-8}$ and $10^{-7}$ M standard solution ($17{\beta}-estradiol$), respectively. G. radix extract showed no cytotoxicity against osteoblast MC3T3-E1 cells at $1{\sim}1,000{\mu}g/ml$. The extract of A. radix showed no significant proliferation of osteoblast. However, the extract of G. radix and D. rhizome showed maximum 148% and 133% proliferation effects. The extract of G. radix also increased alkaline phosphatase activity and the maximum was 122% at $100{\mu}g/ml$ compared to that of control. The nodule formation by the method of the Alizarin red S staining increased compared to control. These results suggest that G. radix is able to perform the bone formation and prevent osteoporosis.

• Food Science and Preservation
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• v.23 no.6
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• pp.876-882
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• 2016

The antioxidant and anti-inflammatory effects of Corni fructus extracts (CEF, EtOAc extraction; CBF, buthanol extraction; CWF, water extraction) were investigated. The total phenolics of CEF (173.3 mg TAE/g) were significantly higher than those of CWF (26.7 mg TAE/g) and CBF (94.8 mg TAE/g). DPPH and ABTS free radical scavenging activity of CEF (DPPH: $RH_{50}$; $25.1{\mu}g/mL$, ABTS: $RC_{50}$; $36.1{\mu}g/mL$) showed even higher than that of BHA and ${\alpha}-tocopherol$ used as positive control. All three Corni fructus extracts in the concentration of $1{\sim}100{\mu}g/mL$ were effective inhibitors of NO and prostaglandin $E_2$ ($PGE_2$). NO production was inhibited 71.3~92.2% by CEF, 76.8~85.5% by CBF and 74.4~96.9% by CWF, respectively. CEF, CBF and CWF ($1{\sim}100{\mu}g/mL$) inhibited also pro-inflammatory cytokines like $TNF-{\alpha}$, $IL-1{\beta}$ and IL-6 very effectively. $TNF-{\alpha}$ was inhibited up to 51.2% by CWF and $IL-1{\beta}$ was inhibited up to 67.1% by CEF. IL-6 was best inhibited by CEF up to 58.9%. This study suggested the potential of Corni fructus for use as an excellent antioxidant substance and inflammatory inhibiting mediators. Therefore CEF, CBF and CWF Corni fructus extracts may be used for therapeutic approach to various inflammatory diseases.

• Journal of Digital Convergence
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• v.14 no.2
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• pp.295-303
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• 2016

This study aimed to assess the influence of nicotine dependence with satisfaction of basic psychological needs, social support, attitude on smoking among middle school students. From Sep, 2015 to Nov, 2015, participants included 150 middle school students from D city and K province in Korea. Data for basic psychological needs, social support, attitude on smoking and nicotine dependence were collected through a self-reported questionnaire and were analyzed with independent t-test and analysis of variance, Pearson's correlation analysis, and multiple linear regression analysis. This study shows negative correlations between nicotine dependence and satisfaction of basic psychological needs(r=-.221, P=.008), competency(r=-.194, P=.021), relatedness(r=-.219, P=.009). The variables predicting nicotine dependence were satisfaction of basic psychological needs(${\beta}=.221$, p=.008). These variables accounted for 42% of the variance of nicotine dependence in smoking middle school students. Our results indicated that it is necessary to increase basic psychological needs to decrease nicotine dependence. Therefore we should develop programs in order to increase satisfaction of basic psychological needs.

• Journal of Digital Convergence
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• v.16 no.1
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• pp.187-195
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• 2018

The purpose of this study was to identify the moderated effects of multicultural contact experience on the relationship between social distance and multicultural acceptability among nursing students. The sample for this study consisted of 174 nursing students from four universities located in Korea. Data were analyzed by descriptive statistics, t-test, ANOVA, $Scheff{\acute{e}}$ test, Pearson correlation and hierarchical multiple regression with SPSS/WIN 23.0. It was found that: (a) multicultural acceptability had a negative correlation with social distance(r=-.61, p<.001), whereas a positive correlation was observed with experience of multicultural contact(r=.24, p=.002); (b) the interaction effect by multicultural contact experience and social distance had a significant influence on the multicultural acceptability(${\beta}=-.18$, p=.003); (c) experience of multicultural contact moderated the relationship between social distance and multicultural acceptability(${\Delta}R^2=0.30$, p=.003). Results of the study suggest that the greater the experience of multicultural contact, the greater the negative effect of social distance on multicultural acceptability. Therefore, to increase the multicultural acceptability of nursing students, it is important to encourage the positive experiences of multicultural contact and thus reduce social distance.

• Journal of Digital Convergence
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• v.15 no.3
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• pp.267-275
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• 2017

The purpose of this study is to examine the effects of work-family conflict factors on depression in Korean married women. For the analysis of the research, we were used in the 5th year data of Korean Longitudinal Survey of Women and Families. The subjects of this study were married women who were living with their husbands and wage workers, and the final analysis was 1,299. As a hierarchical regression analysis result, the work-family conflict of the working married women had a significant effect on depression. Especially, conflict from family to work has the highest effect on depression(${\beta}=.150$, p<0.01). This study suggests that traditional gender role pressures can have a negative impact on the mental health of working women. Therefore, it is necessary to take appropriate social policy measures to alleviate work-family conflicts of married working women.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.12
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• pp.910-917
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• 2018

This paper describes the developments to improve the feeling and safety of the remote control system of unmanned vehicles. Generally, in the case of the remote control systems, a joystick-type device or a simple steering-wheel are used. There are many cases, in which there are operations without considering the feedback to users and driving feel. Recently, as the application area of the unmanned vehicles has been extended, the problems caused by not considering the feedback are emphasized. Therefore, the need for a force feedback-haptic control arises to solve these problems. In this study, the force feedback-haptic control algorithm considering the vehicle parameters is proposed. The vehicle parameters include first the state variables of dynamics, such as the body side-slip angle (${\beta}$) and yawrate (${\gamma}$), and second, the parameters representing the driving situations. Force feedback-haptic control technology consists of the algorithms for general and specific situations, and considers the situation transition process. To verify the algorithms, a simulator was constructed using the vehicle dynamics simulation tool with CAN communication environment. Using the simulator, the feasibility of the algorithms was verified in various scenarios.

• Animal Bioscience
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• v.37 no.3
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• pp.522-535
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• 2024

Objective: Transition period is considered from 3 weeks prepartum to 3 weeks postpartum, characterized with dramatic events (endocrine, metabolic, and physiological) leading to occurrence of production diseases (negative energy balance/ketosis, milk fever etc). The objectives of our study were to analyze the periodic concentration of serum beta-hydroxy butyric acid (BHBA), glucose and oxidative markers along with identification, and validation of the putative markers of negative energy balance in buffaloes using in-silico and quantitative real time-polymerase chain reaction (qRT-PCR) assay. Methods: Out of 20 potential markers of ketosis identified by in-silico analysis, two were selected and analyzed by qRT-PCR technique (upregulated; acetyl serotonin o-methyl transferase like and down regulated; guanylate cyclase activator 1B). Additional two sets of genes (carnitine palmotyl transferase A; upregulated and Insulin growth factor; downregulated) that have a role of hepatic fatty acid oxidation to maintain energy demands via gluconeogenesis were also validated. Extracted cDNA (complementary deoxyribonucleic acid) from the blood of the buffaloes were used for validation of selected genes via qRTPCR. Concentrations of BHBA, glucose and oxidative stress markers were identified with their respective optimized protocols. Results: The analysis of qRT-PCR gave similar trends as shown by in-silico analysis throughout the transition period. Significant changes (p<0.05) in the levels of BHBA, glucose and oxidative stress markers throughout this period were observed. This study provides validation from in-silico and qRT-PCR assays for potential markers to be used for earliest diagnosis of negative energy balance in buffaloes. Conclusion: Apart from conventional diagnostic methods, this study improves the understanding of putative biomarkers at the molecular level which helps to unfold their role in normal immune function, fat synthesis/metabolism and oxidative stress pathways. Therefore, provides an opportunity to discover more accurate and sensitive diagnostic aids.