• Food Science of Animal Resources
• /
• v.41 no.1
• /
• pp.85-94
• /
• 2021

A pig-specific real-time PCR assay based on the mitochondrial ND5 gene was developed to detect porcine material in food and other products. To optimize the performance of assay, seven commercial TaqMan master mixes and two real-time PCR platforms (Applied Biosystems StepOnePlus and Bio-rad CFX Connect) were used to evaluate the limit of detection (LOD) as well as the PCR efficiency and specificity. The LODs and PCR efficiencies for the seven master mixes on two platforms were 0.5-5 pg/reaction and 84.96%-108.80%, respectively. Additionally, non-specific amplifications of DNA from other animal samples (human, dog, cow, and chicken) were observed for four master mixes. These results imply that the sensitivity and specificity of a real-time PCR assay may vary depending on master mix and platform used. The best combination of master mix and real-time PCR platform can accurately detect 0.5 pg porcine DNA, with a PCR efficiency of 100.49%.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.43 no.9
• /
• pp.1325-1333
• /
• 2014

Sasang constitutional medicine is a Korean traditional medicine in which individuals are classified into four constitutional types, Taeyangin, Taeumin, Soyangin, and Soeumin. To develop new functional Saengsik products, we investigated the immunostimulatory activities of raw materials of Saengsik (milled rice, barley, Job's tears, soybean, adzuki bean, millet, eggplants, radish, carrot, Lentinus edodes, Agaricus bisporus, and Auricularia auricula-judae) and Saengsik products (Mix 1~6) in primary immune cells (lymphocytes and macrophages) isolated from blood of Taeumin, Soyangin, and Soeumin types. Increased cell proliferation as well as NO and TNF-${\alpha}$ protein production by immune cells treated with extracts of materials of Saengsik and Saengsik products were measured as immunostimulatory parameters. We chose milled rice, adzuki bean, radish, and Lentinus edodes to study the immunostimulatory activity of Teaumin, barley, soybean, eggplants, and Agaricus bisporus to study the immunostimulatory activity of Soyangin, as well as Job's tears, adzuki bean, carrot, and Auricularia auricula-judae to study the immunostimulatory activity of Soeumin. The mixtures (Mix 1~6) were made with different kinds and ratios of Saengsik materials based on the immunostimulatory activities of Saengsik materials. The immunostimulatory activity of Taeumin was highest in Mix 2 (rice 60%, adzuki bean 20%, radish 10%, and Lentinus edodes 10%) extract-treated immune cells among all six mixtures. The immunostimulatory activity of Soyangin responded best to Mix 4 (barley 60%, soybean 20%, eggplants 10%, and Agaricus bisporus 10%) among the six extracts (Mix 1~6). The increased immunostimulatory activity of Mix 6 (Job's tears 60%, adzuki bean 20%, carrot 10%, and Auricularia auricula-judae 10%)-treated immune cells was higher than the other five extracts (Mix 1~5). Accordingly, Mix 2, Mix 4, and Mix 6 may be useful as mixtures for Saengsik products having personalized immunostimulatory activities.

• Journal of the Korean Society of Fashion and Beauty
• /
• v.4 no.2 s.8
• /
• pp.6-14
• /
• 2006

The purpose of this study is to examine how the Chanel Collection, a famous brand acknowledged for it's modern style, was influenced by post-modernism. The scope of research includes initial concentration on 1920's boyish style of Chanel as well as its relative influence on modernism. Secondly, the research concentrates on Chanel Collections that Lagerfeld's designs were best represented. The results were as follows: The tendency to eroticism for fashion business, tendency to retro-fashion, tendency to popularization of sub-cultural group's fashion, tendency to no-match-mix, tendency to androgynous style, tendency to emphasis on ornaments in clothing.

• Proceedings of the Korean Institute of Building Construction Conference
• /
• 2018.05a
• /
• pp.138-139
• /
• 2018

This study is a basic review of the basic characteristics of mortar as a result of the use of concrete as a fine aggregate for CGS(coal gasification slag) generated from the IGCC(integrated gasification combined cycle). The analysis shows that CGS and crushed sand + seal sand mix is the best combination of CGS combined with about 75 % of CGS based on the effects of promoting liquidity and strength. This is expected to be a positive factor in securing the strength and flexibility of concrete given the optimal mix of CGS, and may also contribute to the improvement of quality.

• Proceedings of the Korean Institute of Building Construction Conference
• /
• 2018.11a
• /
• pp.159-160
• /
• 2018

In this study, the characteristics of the Mock-up test were reviewed to analyze the applicability of the coal gasification slag (CGS) from the integrated gasification combination Cycle (IGCC) to the concrete fine aggregate. The analysis shows that CGS and crushed sand mix is the best combination of CGS combined with about 50 % of CGS based on the effects of promoting liquidity and strength. This is expected to be a positive factor in securing the strength and flexibility of concrete given the optimal mix of CGS, and may also contribute to the improvement of quality.

• Proceedings of the Korean Institute of Building Construction Conference
• /
• 2018.11a
• /
• pp.21-22
• /
• 2018

In this study, the characteristics of the Mock-up test were reviewed to analyze the applicability of the coal gasification slag (CGS) from the integrated gasification combination Cycle (IGCC) to the concrete fine aggregate. The analysis shows that CGS and crushed sand mix is the best combination of CGS combined with about 50 % of CGS based on the effects of promoting liquidity and strength. This is expected to be a positive factor in securing the strength and flexibility of concrete given the optimal mix of CGS, and may also contribute to the improvement of quality.

• Proceedings of the Korea Concrete Institute Conference
• /
• 2006.11a
• /
• pp.689-692
• /
• 2006

The crack of concrete induced by the heat of hydration is a serious problem, particularly in massive concrete structures. In order to control the temperature crack of massive concrete, the selection of appropriate materials like low heat cement, mixture materials, etc. is essential. In tills study, mix proportion using low heat portland cement and lime stone powder was designed and the best mix proportion, B-1, was selected. When bottom slab of the #219 LNG tank in Incheon was constructed, concrete temperature was measured. And thermal stress was analyzed about bottom slab of the LNG tank. As results of the thermal analysis, crack index was 1.60 in bottom slab and satisfied with construction specifications(over 1.0).

• Proceedings of the Korean Institute of Building Construction Conference
• /
• 2011.05a
• /
• pp.131-132
• /
• 2011

This study analyzes the yearly-best delivered size range of truck mixer based on the specified mix, Water to Binder Ratio, aggregate proportion and unit amount with statistical method targeting on Kyeongi and Kyeongsang province and compares with the similar materials of Japan to propose as a basic standard for the quality control of mixer truck. As a result, in case of the Water to Binder Ratio of these areas, it is higher than Japan's due to the excessive safety rate reflecting the changes of differential value impact and unit amount, and the unit amount's standard deviation is very large by reflecting the changes of the amount used and chemical admixture susceptibility. In case of aggregate proportion, the frequency rate is about 50%, which is very similar value with Japan's one.

• Environmental and Resource Economics Review
• /
• v.28 no.2
• /
• pp.201-229
• /
• 2019

This paper estimates the effects of generation mix changes in the $7^{th}$ and $8^{th}$ Basic Plan for Long-term Power Supply and Demand from two aspects: economic efficiency through electricity prices and environmental performance through $CO_2$ and air pollutants(NOx, SOx, PM) emissions. Particularly, we examined additional generation mix conversion paths that take into account the trade-off between economic efficiency and environmental performance through scenario analysis. According to our results, the conversion from the $7^{th}$ plan to the $8^{th}$ plan should increase the electricity prices in the mid- and long-term, while reducing GHG and air pollutants emissions at the same time. The alternative generation mix that combines $7^{th}$ and $8^{th}$ plans shows that there exists a path to mitigate the trade-off between economic and environmental in the long-term. It will be next to impossible to derive a optimal generation mix that simultaneously considers the core values, such as supply stability, environmental performance, economic efficiency, energy safety and energy security, when establishing the power supply and demand plan. However, by exploring the effects of various generation mix paths and suggesting near-optimal paths, people can best choose their direction after weighhing all the paths when deciding on a forward-looking generation mix in the long term.

• Composite Materials and Engineering
• /
• v.3 no.3
• /
• pp.201-220
• /
• 2021

In this study, potential of three machine learning techniques i.e., M5P, Support vector machines and Gaussian processes were evaluated to find the best algorithm for the prediction of flexural strength of concrete mix with steel fibre. The study comprises the comparison of results obtained from above-said techniques for given dataset. The dataset consists of 124 observations from past research studies and this dataset is randomly divided into two subsets namely training and testing datasets with (70-30)% proportion by weight. Cement, fine aggregates, coarse aggregates, water, super plasticizer/ high-range water reducer, steel fibre, fibre length and curing days were taken as input parameters whereas flexural strength of the concrete mix was taken as the output parameter. Performance of the techniques was checked by statistic evaluation parameters. Results show that the Gaussian process technique works better than other techniques with its minimum error bandwidth. Statistical analysis shows that the Gaussian process predicts better results with higher coefficient of correlation value (0.9138) and minimum mean absolute error (1.2954) and Root mean square error value (1.9672). Sensitivity analysis proves that steel fibre is the significant parameter among other parameters to predict the flexural strength of concrete mix. According to the shape of the fibre, the mixed type performs better for this data than the hooked shape of the steel fibre, which has a higher CC of 0.9649, which shows that the shape of fibers do effect the flexural strength of the concrete. However, the intricacy of the mixed fibres needs further investigations. For future mixes, the most favorable range for the increase in flexural strength of concrete mix found to be (1-3)%.