• Food Science of Animal Resources
• /
• v.26 no.4
• /
• pp.426-430
• /
• 2006

This study was conducted to investigate the effects of gender on sensory scores, fatty acid and amino acid of Berkshire pork A total of 40 pigs (60 kg) were divided into 2 group (gilt and barrow) and raised up to 110 kg live weight. Pigs were conventionally slaughtered, and then chilled overnight. Pork loin (longissimus dorsi) muscle was removed from each left side and meat qualities were evaluated. The results obtained were as follows; In sensory evaluation, color, marbling score, aroma and overall acceptability of brrow groups were significantly higher than that of gilt groups (p<0.05). In fatty acid analysis, linoleic acid and arachidonic acid of gilt groups were higher than those of barrow groups, EFA (essential fatty acid) and UFA (unsaturated fatty acid) were lower in barrow groups compared to the gilt groups. In amino acid compositions, alanine, tyrosine and arginine of barrow groups were significantly higher and threonine, proline and histidine lower than those of gilt groups (p<0.05).

• Food Science of Animal Resources
• /
• v.27 no.2
• /
• pp.137-141
• /
• 2007

This study was conducted to investigate the proximate composition and physico-chemical characteristics of Berkshire pork by gender. A total of 40 pigs(60 kg) were divided into 2 groups(gilt and barrow) and raised to 110 kg of live weight. pigs were conventionally slaughtered, and then chilled overnight. Pork loin(longissimus dorsi) muscle was removed from each left side and meat qualities were evaluated. The proximate composition was no different between genders, and the pH was higher iii the barrow group(p<0.05). The water holding capacity, cooking loss, shear force, and cholesterol content were no different between genders. With regard to meat color, the $L^*$ value was higher in the barrow group(p<0.05), but the $a^*\;and\;b^*$ values were no different between genders. The fat color was also no different between genders. The texture properties, brittleness, hardness, and gumminess of the gilt group were higher than those of barrow group(p<0.05), but cohesiveness, springiness, and chewiness were no different. In conclusion, the pH and $L^*$ values of the barrow group were higher than those of gilt group, and tenderness was higher in the barrow group.

• Journal of Animal Science and Technology
• /
• v.46 no.3
• /
• pp.307-314
• /
• 2004

Xenotransplantation of porcine organs has the potential to overcome the severe. shortage of human tissues and organs available for human transplantation. The swine represents an ideal source of such organs because of their plentiful supply and their numerous anatomical and physiological similarities to the human. However, this procedure also carries with a number of safety issues relating to the zoonotic infections. Porcine endogenous retrovinJses(PERVs), \Wich are germ line transmitted and persist without symptoms in the pigs, are most concerning zoonotic viroses. In order to analyze the prevalence of PERV in domestic pigs, four kinds of pigs'(Landrace, Berkshire, Yorkshire, and Duroc) genomic DNA were isolated from their hair follicles. PCR analysis was carried out for detection of PERVs using subgroup A/B/C and E pol sequence primers. All pigs (20 heads) tested had high copy number of PERVs within genomes. Subgroup A/B/C and E pol gene sequences from 20 isolates were determined by direct sequencing. Sequence analysis showed pol sequences are highly conserved among intra- and inter-subspecies(99.l and 98.8%, respectively). As a first report of PERV prevalence in Korea pigs, our data would be the basic concepts of PERV transmission study in xenotransplantation.

• Asian-Australasian Journal of Animal Sciences
• /
• v.30 no.5
• /
• pp.749-754
• /
• 2017

Objective: The objective of the present study was to investigate the effects of farrowing month (FM), parity and sex on the growth performance of Berkshire swine raised in alternative production systems. Methods: A total of 40 farrowing records from 27 sows and 1,258 body weight (BW) records from 274 piglets collected over a two-year period were used for the analysis. The BWs were recorded at birth, weaning (28 d), 56, 84, 112, and 140 days. Any BW not recorded on schedule was recalculated to conform the days of age among corresponding BW records, using growth curves drawn with polynomial functions whose power was determined by the number of existing observations for each individual. Results: The mean parity (${\pm}$standard deviation) of the sows was $3.42{\pm}2.14$. The sows that farrowed in June had the lowest number of total born with an average of $6.25{\pm}2.22$ piglets per sow. However, the lowest average number of piglets weaned at day 28 was found in sows that farrowed in May, as well as the highest number recorded for the stillborn piglets with an average of 2.67 piglets per sow. Moreover, the smallest increase in weight from birth to weaning occurred in piglets that were farrowed in May, which also corresponds with the average daily gain (ADG) of 0.29 kg and the last recorded weight measurement on day 140 of $41.69{\pm}1.45kg$. Contrastingly, the highest growth rate was found among pigs farrowed in June, with the largest increase in weight of 7.55 kg from birth to weaning, the highest ADG of 0.51 kg from birth to 140 day of age and the highest BW of $74.70{\pm}1.86kg$ recorded on day 140. Conclusion: Pigs farrowed in June also had the least number of piglets that died between birth and weaning. The zone of thermal comfort found in sows reared in indoor confinement systems did not improve the reproductive performance of the sows reared in an outdoor, alternative production system, while the growth performance of the piglets was improved when the ambient temperature was consistently hot or consistently cold.

• Asian-Australasian Journal of Animal Sciences
• /
• v.25 no.10
• /
• pp.1357-1363
• /
• 2012

Pigs may need to be exploited as xenotransplantation donors due to the shortage of human organs, tissues and cells. Porcine endogenous retroviruses (PERVs) are a significant obstacle to xenotransplantation because they can infect human cells in vitro and have the potential for transmission of unexpected pathogens to humans. In this research, 101 pigs, including four commercial breeds (23 Berkshire, 13 Duroc, 22 Landrace and 14 Yorkshire pigs), one native breed (19 Korean native pigs) and one miniature breed (10 NIH miniature pigs) were used to investigate insertional variations for 11 PERV loci (three PERV-A, six PERV-B and two PERV-C). Over 60% of the pigs harbored one PERV-A (907F8) integration and five PERV-B (B3-3G, B3-7G, 742H1, 1155D9 and 465D1) integrations. However, two PERV-A loci (A1-6C and 1347C1) and one PERV-B locus (B3-7F) were absent in Duroc pigs. Moreover, two PERV-C loci (C2-6C and C4-2G) only existed in Korean native pigs and NIH miniature pigs. The results suggest that PERV insertional variations differ among pig breeds as well as among individuals within a breed. Also, the results presented here can be used for the selection of animals that do not have specific PERV integration for xenotransplantation research.

• Food Science of Animal Resources
• /
• v.37 no.6
• /
• pp.926-930
• /
• 2017

High-quality meat is of great economic importance to the pig industry. The 1-acylglycerol-3-phosphate-O-acyltransferase 5 (AGPAT5) enzyme converts lysophosphatidic acid to phosphatidic acid in the mitochondrial membrane. In this study, we found that the porcine AGPAT5 gene was highly expressed in muscle tissue, influencing meat characteristics, and we also identified a non-synonymous single-nucleotide polymorphism (nsSNP) (rs196952262, c.673 A>G) in the gene, associated with a change of isoleucine 225 to valine. The presence of this nsSNP was significantly associated with meat color (lightness), lower cooking loss, and lower carcass temperatures 1, 4, and 12 h after slaughter (items T1, T4, and T12 on the recognized quality scale, respectively), and tended to increase backfat thickness and the water-holding capacity. These results suggest that nsSNP (c.673A>G) of the AGPAT5 gene is a potential genetic marker of high meat quality in pigs.

• Journal of Animal Science and Technology
• /
• v.49 no.3
• /
• pp.379-386
• /
• 2007

This study was conducted to investigate the effect of the mating system on the retail cut yield and meat quality in pigs. A total of 210 samples were used to estimate meat productivity and meat quality two blocks of two boarlines Duroc (D) and Berkshire (B) were mated to sows of the sowlines Yorkshire×Landrace (YL) and Berkshire (B). Results showed that YLD (YL♀×D♂) and BD (B♀×D♂) had higher percentages of Boston butt and belly than YLB while it has higher percentages of lean meat yield than YLB (YL♀×B♂). There were no significant differences in crude protein and ash content of pork from different breeds (P>0.05). However, YLB and YLD had higher moisture content in loin and Boston butt than BD (P<0.05). The pH of BD was highest in pork loin and belly (P<0.05). There differences in meat yield, pH and moisture content among the genotypes suggested that pork quality might be affected by the lightness and cooking loss. BD was lowest lightness value (CIE L*) and cooking loss in pork loin among the genotypes (P<0.05). The percentage of saturated palmitic acid (C16:0) in Boston butt (P<0.05) of BD had significant lower, while the percentage of unsaturated palmitoleic (C16:1) and oleic acid (C18:1) had higher in pork loin and belly (P<0.05). Drip and marbling score were found significantly different in genotypes and BD had lower drip and higher marbling score in pork loin and belly (P<0.05). The sensory evaluations indicated that the highest overall acceptability in BD was attained. From the results, BD breed had highest meat quality among crossbred pigs.

• Asian-Australasian Journal of Animal Sciences
• /
• v.28 no.11
• /
• pp.1551-1557
• /
• 2015

The missing heritability has been a major problem in the analysis of best linear unbiased prediction (BLUP). We introduced the traditional genome-wide association study (GWAS) into the BLUP to improve the heritability estimation. We analyzed eight pork quality traits of the Berkshire breeds using GWAS and BLUP. GWAS detects the putative quantitative trait loci regions given traits. The single nucleotide polymorphisms (SNPs) were obtained using GWAS results with p value <0.01. BLUP analyzed with significant SNPs was much more accurate than that with total genotyped SNPs in terms of narrow-sense heritability. It implies that genomic estimated breeding values (GEBVs) of pork quality traits can be calculated by BLUP via GWAS. The GWAS model was the linear regression using PLINK and BLUP model was the G-BLUP and SNP-GBLUP. The SNP-GBLUP uses SNP-SNP relationship matrix. The BLUP analysis using preprocessing of GWAS can be one of the possible alternatives of solving the missing heritability problem and it can provide alternative BLUP method which can find more accurate GEBVs.

• Journal of Animal Science and Technology
• /
• v.49 no.2
• /
• pp.195-202
• /
• 2007

The growth traits in swine are economically important, which are measured by gain in weight during test period or by age of days to certain weight. However, the difference in growth rate due to individual performance and also other factors occurs. The more reasonable estimation of the measurements of these traits provides the less error in genetic evaluation of pigs. The data from 1,576 heads being weighed periodically of Gyungnam-heugdon(Berkshire) were analyzed to estimate the growth curve which is used to decide average daily gain and days to 90kg. It may not be possible to directly compare accuracy between the conventional methods and the alternative methods. However, the alternative methods by growth curve would be superior to the conventional methods not only in theoretical background, but also in acceptability for diverse factors such as breed, sex and age. The theoretical superiority of the alternative methods comes from estimation at same age in daily gain and calculation of additional days from measuring date to days to 90kg by growth curve of individual. Also this can be easily adopted in a computer system according to breed and sex.

• Asian-Australasian Journal of Animal Sciences
• /
• v.21 no.2
• /
• pp.155-160
• /
• 2008

Several studies have reported quantitative trait loci (QTL) for meat quality on porcine chromosome 2 (http://www.animalgenome.org/QTLdb/pig.html). For application of the molecular genetic information to the pig industry through marker-assisted selection, single nucleotide polymorphism (SNP) markers were analyzed by comparative re-sequencing of polymerase chain reaction (PCR) products of 13 candidate genes with DNA from commercial pig breeds such as Berkshire, Yorkshire, Landrace, Duroc and Korean Native pig. A total of 34 SNPs were identified in 15 PCR products producing an average of one SNP in every 253 bp. PCR restriction fragment length polymorphism (RFLP) assays were developed for 11 SNPs and used to investigate allele frequencies in five commercial pig breeds in Korea. Eight of the SNPs appear to be fixed in at least one of the five pig breeds, which indicates that different selection among pig breeds might be applied to these SNPs. Polymorphisms detected in the PTH, CSF2 and FOLR genes were chosen to genotype a Berkshire-Yorkshire pig breed reference family for linkage and association analyses. Using linkage analysis, PTH and CSF2 loci were mapped to pig chromosome 2, while FOLR was mapped to pig chromosome 9. Association analyses between SNPs in the PTH, CSF2 and FOLR suggested that the CSF2 MboII polymorphism was significantly associated with several pork quality traits in the Berkshire and Yorkshire crossed F2 pigs. Our current findings provide useful SNP marker information to fine map QTL regions on pig chromosome 2 and to clarify the relevance of SNP and quantitative traits in commercial pig populations.