• Title/Summary/Keyword: Bench-scale

Search Result 353, Processing Time 0.025 seconds

Realization of the national standard of candela traceable to the absolute cryogenic radiometer at KRISS (극저온 절대복사계에 소급한 칸델라 국가표준 실현)

  • Park, Seung-Nam;Kim, Yong-Wan;Lee, Dong-Hoon
    • Korean Journal of Optics and Photonics
    • /
    • v.15 no.5
    • /
    • pp.443-448
    • /
    • 2004
  • We realized the national standard of the candela, one of the SI units, by using two photometers with the spectral responsivity measured in reference to the absolute cryogenic radiometer. The external apertures of the photometers were fabricated using a diamond turning machine, and measured in terms of area with uncertainty of 0.05 %(k = 1). The candela is realized using a 1 kW FEL lamp and the characterized photometers on an optical bench. The uncertainty is budgeted to be 0.25 %(k = 1) considering the uncertainty of the spectral responsivity and the response uniformity of the detectors, the area of the external apertures, the color temperature of the lamp, and the positioning reproducibility of the photometers and the lamp. We verified the realized scale by comparing with the scale of National Institute of Standards and Technology, USA. They coincided with each other within 0.1%.

Effect of Culture Conditions and Signal Peptide on Production of Human Recombinant N-Acetylgalactosamine-6-Sulfate Sulfatase in Escherichia coli BL21

  • Hernandez, Alejandra;Velasquez, Olga;Leonardi, Felice;Soto, Carlos;Rodriguez, Alexander;Lizaraso, Lina;Mosquera, Angela;Bohorquez, Jorge;Coronado, Alejandra;Espejo, Angela;Sierra, Rocio;Sanchez, Oscar F.;Almeciga-Diaz, Carlos J.;Barrera, Luis A.
    • Journal of Microbiology and Biotechnology
    • /
    • v.23 no.5
    • /
    • pp.689-698
    • /
    • 2013
  • The production and characterization of an active recombinant N-acetylgalactosamine-6-sulfate sulfatase (GALNS) in Escherichia coli BL21(DE3) has been previously reported. In this study, the effect of the signal peptide (SP), inducer concentration, process scale, and operational mode (batch and semi-continuous) on GALNS production were evaluated. When native SP was presented, higher enzyme activity levels were observed in both soluble and inclusion bodies fractions, and its removal had a significant impact on enzyme activation. At shake scale, the optimal IPTG concentrations were 0.5 and 1.5 mM for the strains with and without SP, respectively, whereas at bench scale, the highest enzyme activities were observed with 1.5 mM IPTG for both strains. Noteworthy, enzyme activity in the culture media was only detected when SP was presented and the culture was carried out under semi-continuous mode. We showed for the first time that the mechanism that in prokaryotes recognizes the SP to mediate sulfatase activation can also recognize a eukaryotic SP, favoring the activation of the enzyme, and could also favor the secretion of the recombinant protein. These results offer significant information for scaling-up the production of human sulfatases in E. coli.

The Effect of Street Gardens on Psychological Restoration (도심 가로정원의 심리적 회복효과에 관한 연구)

  • Kwon, Hyun-Sook;Hahm, Yean-Kyoung;Kim, Hae-Ryung;Yoon, Hee-Yeun
    • Journal of the Korean Institute of Landscape Architecture
    • /
    • v.45 no.1
    • /
    • pp.35-51
    • /
    • 2017
  • Street gardens, a series of streetscape improvement projects led by Seoul City Government, are initiated for the purpose of providing aesthetic satisfaction and mental refreshment to pedestrians. In order to investigate whether street gardens indeed promote the psychological health of the users, questionnaire surveys were conducted on three selected street gardens - at Gangnam-daero, Digital-ro, and Teheranro - and their comparison sites located on the same streets, which have a similar physical environment but without a street garden. The survey questionnaires, based on Attention Restoration Theory, were composed of Perceived Restorativeness Scale-11 with the eleven individual questions grouped into four categories: 'Fascination', 'Being away', 'Coherence', and 'Scope'. The survey questionnaires also ask about physical components that promote psychological improvement in the aforementioned categories. The collected data was analyzed with factor analysis, reliability analysis, and independent t-test. The results suggested that street gardens had a relatively positive effect on the psychological restorativeness of the users. In particular, they gave fascination and interest to the users. However, they did not offer a feeling of being away to the users, which revealed the limitation in the psychological improvement effect of street gardens. The physical components of the street garden that have led the psychological restorativeness effect were wooden bench, tree, and flower. This result corresponds to an extant theory that natural factors have a positive effect on the psychological restorativeness within a hardscape. This research will shed light on the planning and design guidelines for the street garden project.

Global Trend of CO2 Capture Technology Development (이산화탄소 포집기술 국외 기술개발 동향)

  • Baek, Jeom-In
    • KEPCO Journal on Electric Power and Energy
    • /
    • v.2 no.2
    • /
    • pp.143-165
    • /
    • 2016
  • The amount of greenhouse gas emission reduction based on INDCs (Intended Nationally Determined Contributions) submitted to UN by each party is not sufficient to achieve the Paris Agreement's aim to "hold the increase in the global average temperature to well below $2^{\circ}C$ above pre-industrial levels and to pursue efforts to limit the temperature increase to $1.5^{\circ}C$" which was determined in the $21^{st}$ Conference of the Parties to the UNFCCC (COP 21). Accordingly, the emission reduction target of each party will be revised for the $2^{\circ}C$ goal. Among the several options to reduce the carbon emission, CCS (Carbon Capture and Storage) is a key option to curb $CO_2$ emissions from large emission sources such as fossil-based power plants, cement plants, and steel production plants. A large scale CCS demonstration projects utilizing $1^{st}$ generation $CO_2$ capture technologies are under way around the world. It is anticipated, however, that the deployment of those $1^{st}$ generation $CO_2$ capture technologies in great numbers without government support will be difficult due to the high capture cost and considerable increase of cost of electricity. To reduce the carbon capture cost, $2^{nd}$ and $3^{rd}$ generation technologies are under development in a pilot or a bench scale. In this paper, current status of large scale CCS demonstration projects and the $2^{nd}$ and $3^{rd}$ generation capture technologies are summarized. Novel capture technologies on wet scrubbing, dry sorbent, and oxygen combustion are explained in detail for all capture areas: post-combustion capture, pre-combustion capture, and new combustion technologies.

Current Status and Prospect of Seaweed-based Biofuels as Renewable Energy Resource (재생가능 에너지원으로서의 해조류 유래 바이오 연료의 현황과 전망)

  • Liu, Jay
    • Clean Technology
    • /
    • v.28 no.2
    • /
    • pp.163-173
    • /
    • 2022
  • Research and development of biofuels as one of the means to mitigate global warming and to avoid fossil fuel depletion has occurred for more than 30 years. However, there has only been limited distribution of a few first- and second-generation biofuels, and widespread supply and consumption of biofuels is still far from a reality. Although a relatively recently studied third-generation biofuel derived from seaweed biomass has been shown to have many advantages, it is yet to be deployed in commercial-scale seaweed biorefineries. This review paper examines the advantages and disadvantages of seaweed biorefineries for the entire value chain covering from seaweed and its cultivation to biofuel production based on an extensive literature search and the author's experience of conducting feasibility studies pertaining to seaweed biorefineries for over 10 years. For this purpose, the literature survey will cover the current status of seaweed production and its research and development worldwide, conversion technologies for biofuel production from seaweed based on bench-scale experiments, and large-scale techno-economic feasibility studies for seaweed conversion to biofuels and bioenergy. In addition, the main problems expected with the commercialization of seaweed-based biofuels will be identified. Finally, the current status of seaweed biorefinery technology and the author's views on its promising future will be summarized.

Decrease in the Thickness of Capillary Fringe Induced by Surface Active Chemicals in the Groundwater (계면활성물질의 지하수적용에 의한 모관수대 두께의 감소)

  • Kim, Heonki;Shin, Seungyup;Yang, Haewon
    • Journal of Soil and Groundwater Environment
    • /
    • v.17 no.6
    • /
    • pp.52-58
    • /
    • 2012
  • Capillary fringe divides the groundwater and the vadose zone controlling the diffusive mass transfer of contaminants and gases. The thickness of capillary fringe is of great importance for the rate of contaminant mass transfer across the capillary fringe. Application of surface active chemicals including surfactants and alcohol-based products into the subsurface environment changes the surface tension of the aqueous phase, which in turn, affects the thickness of the capillary fringe. In this study, a bench-scale model was used to assess the quantitative relationship between the surface tension and the thickness of the capillary fringe. An anionic surfactant (Sodium dodecylbenzene sulfonate, SDBS) and an aqueous solution of ethanol were used to control the surface tension of the groundwater. It was found that the thickness of the capillary fringe is directly proportional to the surface tension. The air entry pressures measured by the Tempe Pressure Cell at different surface tensions using SDBS (200 mg/L) and ethanol (20%, v/v) solutions were in good agreement with the thicknesses of the capillary fringe measured by the model. A simple method to correct the conventional Brooks-Corey model for estimating the air entry pressure was also presented.

Effects of triaxial magnetic field on the anisotropic nanoplates

  • Karami, Behrouz;Janghorban, Maziar;Tounsi, Abdelouahed
    • Steel and Composite Structures
    • /
    • v.25 no.3
    • /
    • pp.361-374
    • /
    • 2017
  • In this study, the influences of triaxial magnetic field on the wave propagation behavior of anisotropic nanoplates are studied. In order to include small scale effects, nonlocal strain gradient theory has been implemented. To study the nanoplate as a continuum model, the three-dimensional elasticity theory is adopted in Cartesian coordinate. In our study, all the elastic constants are considered and assumed to be the functions of (x, y, z), so all kind of anisotropic structures such as hexagonal and trigonal materials can be modeled, too. Moreover, all types of functionally graded structures can be investigated. eigenvalue method is employed and analytical solutions for the wave propagation are obtained. To justify our methodology, our results for the wave propagation of isotropic nanoplates are compared with the results available in the literature and great agreement is achieved. Five different types of anisotropic structures are investigated in present paper and then the influences of wave number, material properties, nonlocal and gradient parameter and uniaxial, biaxial and triaxial magnetic field on the wave propagation analysis of anisotropic nanoplates are presented. From the best knowledge of authors, it is the first time that three-dimensional elasticity theory and nonlocal strain gradient theory are used together with no approximation to derive the governing equations. Moreover, up to now, the effects of triaxial magnetic field have not been studied with considering size effects in nanoplates. According to the lack of any common approximations in the displacement field or in elastic constant, present theory has the potential to be used as a bench mark for future works.

An Evaluation on Citizens' Satisfaction with the Outdoor Landscape Lighting in Gyeongju Historic Areas - The Case of Wolseong District -

  • Park, So-Yeong;Heo, Sang-Hyun
    • Journal of the Korean Institute of Landscape Architecture
    • /
    • v.38 no.5_2
    • /
    • pp.186-193
    • /
    • 2010
  • The purpose of this study was to survey the citizens for their satisfaction with the outdoor landscape lighting in Gyeongju Historic Areas registered as UNESCO World Cultural Heritage in November 2000 and thereupon, provide for some basic data useful to the design of the outdoor landscape lighting for the cultural properties. As a result of examining the conditions of the outdoor landscape lighting in Wolseong Zone of Anapji, Banwolseong, Dongbu Sajeokji, Cheomseongdae and Gyerim, there were found 391 lighting fixtures of 12 types in Anapji, 138 lightings of 4 types in Banwolseong, 38 lightings of 6 types in Cheomseongdae, 28 lightings of 3 types in Dongbu Sajeokji and 54 lightings of 5 types in Gyerim. As a result of analyzing citizens' satisfaction with the outdoor landscape lighting, it was found that citizens were satisfied more or less with the nightscape image changed by the outdoor landscape lighting; their satisfaction scored 3.836 on average for Anapji on a 5-point Likert type scale, 3.516 for Banwolseong, 3.446 for Dongbu Sajeokji, 3.650 for Cheomseongdae and 3.479 for Gyerim. However, citizens' satisfaction with the originality of the nightscape was generally low: 3.055 for Anapji, 2.914 for Cheomseongdae, 2.877 for Banwolseong, 2.847 for Gyerim and 2.665 for Dongbu Sajeokji. On the other hand, since most of the lighting fixtures were installed as inserted lights or floodlights, the color tones of light source were relatively highly distinctive, but the peripheral spaces around the cultural properties were rather dark, which means that citizens were feeling inconvenient more or less for using the amenities such as bench or waste box. All in all, their satisfaction with the outdoor landscape at the sample zone at night scored 2.981, lower than the normal level.

Modal and Stress Analysis of Spur Gear in DC Motor Gearhead using Finite Element Model

  • Pratama, Pandu Sandi;Supeno, Destiani;Jeong, Seongwon;Park, Cunsook;Woo, Jihee;Lee, Eunsook;Yoon, Woojin;Choi, Wonsik
    • Proceedings of the Korean Society for Agricultural Machinery Conference
    • /
    • 2017.04a
    • /
    • pp.17-17
    • /
    • 2017
  • In electric agricultural machine the gearhead is needed to convert the high speed low torque rotation motion generated by DC motor to lower speed high torque motion used by the vehicle. The gearhead consist of several spur gears works as reduction gears. Spur gear have straight tooth and are parallel to the axis of the wheel. Spur gears are the most easily visualized gears that transmit motion between two parallel shafts and easy to produce. The modeling and simulation of spur gears in DC motor gearhead is important to predict the actual motion behavior. A pair of spur gear tooth in action is generally subjected to two types of cyclic stress: contact stress and bending stress including bending fatigue. The stress may not attain their maximum values at the same point of contact fatigue. These types of failure can be minimized by analysis of the problem during the design stage and creating proper tooth surface profile with proper manufacturing methods. To improve its life expectation in this study modal and stress analysis of gearhead is simulated using ansys work bench software based on finite element method (FEM). The modal analysis was done to understand gearhead deformation behaviour when vibration occurs. FEM static stress analysis is also simulated on gearhead to simulate the gear teeth bending stress and contact stress behavior. This methodology serves as an approach for gearhead design evaluation, and the study of gear stress behavior in DC motor gearhead which is needed in the small workshop scale industries.

  • PDF

Polyvinyl Alcohol 분해 공생 균주에 의한 염색 폐수 중의 PVA 제거

  • Kim, Chul Ki;Choi, Yong-Jin;Lee, Chul-Woo;Rim, Yeon-Taek;Ryu, Jae-Keun
    • Microbiology and Biotechnology Letters
    • /
    • v.25 no.1
    • /
    • pp.89-95
    • /
    • 1997
  • The current processer of the textile wastewater treatment are mostly consisted of a combination of a physico-chemical and a biological treatment. The overall efficiency of these processes is, however, assessed to be fairly low. It is even worse during the summer season when temperature of the wastewater rises above 40$\circ $C. Therefore, a feasible process of the textile wastewater treatment which can work efficiently at higher temperatures was investigated in this work. We used a bench scale reactor consisted of one 4 liter anaerobic and one 8 liter aerobic tank, and the thermophilic symbiotic PVA degraders, Pasteruella hemolytica KMG1 and Pseudomonas sp. KMG6 that had been isolated in our laboratory. In the preliminary flask experiments, we observed that the thermophilic symbiotic PVA degraders could not grow in the wastewater substrate. Then, we isolated the mutant strains by acclimating the KMG1and KMG6 strains to the wastewater medium. The mutant symbionts (KMG1-1 and KMG6-1) were isolated through 6 times successive transfers into the fresh wastewater medium after 5 days culture for each. The mutant strains obtained grew well in the mixed medium composed of 75% wastewater and 25% synthetic medium, and supplemented with 0.5% PVA as a sole carbon source. During the culture for 14 days at pH 7.0 and 40$\CIRC $C, the bacteria assimilated about 89% of the added PVA. The symbionts degraded equally well all the PVA substrates of different molecular weight (nd=500~30000). In contrast to the flask experiments, in the reactor system the mutant strains showed very low levels of the PVA and COD removal rates. However, the new reactor system with an additional aerobic tank attained 82% removal rate of COD, 94% of PVA degradation and 71% of color index under the conditions of 5% inoculm on the tank 2, incubation temperature of 40$\circ $C, dissolved oxygen level of 2~3 mg/l and retention time of 30 hours. This result ensures that the process described above could be an efficient and feasible treatment for the PVA contained textile wastewater at higher temperatures.

  • PDF