• 제목/요약/키워드: Beef Traits

검색결과 189건 처리시간 0.028초

Identification of Exonic Nucleotide Variants of the Thyroid Hormone Responsive Protein Gene Associated with Carcass Traits and Fatty Acid Composition in Korean Cattle

  • Oh, Dong-Yep;Lee, Yoon-Seok;La, Boo-Mi;Lee, Jea-Young;Park, Yong-Soo;Lee, Ji-Hong;Ha, Jae-Jung;Yi, Jun-Koo;Kim, Byung-Ki;Yeo, Jung-Sou
    • Asian-Australasian Journal of Animal Sciences
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    • 제27권10호
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    • pp.1373-1380
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    • 2014
  • The thyroid hormone responsive protein (THRSP) gene is a functional gene that can be used to indicate the fatty acid compositions. This study investigates the relationships of exonic single nucleotide polymorphisms (SNPs) in the THRSP gene and fatty acid composition of muscle fat and marbling score in the 612 Korean cattle. The relationships between fatty acid composition and eight SNPs in the THRSP gene (g.78 G>A, g.173 C>T, g.184 C>T, g.190 C>A, g.194 C>T, g.277 C>G, g.283 T>G and g.290 T>G) were investigated, and according to the results, two SNPs (g.78 G>A and g.184 C>T) in exon 1 were associated with fatty acid composition. The GG and CC genotypes of g.78 G>A and g.184 C>T had higher unsaturated fatty acid (UFA) and monounsaturated fatty acid (MUFA) content (p<0.05). In addition, the $ht1^*ht1$ group (Val/Ala haplotype) in a linkage disequilibrium increased MUFAs and marbling scores for carcass traits (p<0.05). As a result, g.78 G>A and g.184 C>T had significantly relationships with UFAs and MUFAs. Two SNPs in the THRSP gene affected fatty acid composition, suggesting that GG and CC genotypes and the $ht1^*ht1$ group (Val/Ala haplotype) can be markers to genetically improve the quality and flavor of beef.

Comparison of Physicochemical and Functional Traits of Hanwoo Steer Beef by the Quality Grade

  • Lim, Dong-Gyun;Cha, Ju-Su;Jo, Cheorun;Lee, Kyung Haeng;Kim, Jong-Ju;Nam, Ki-Chang
    • 한국축산식품학회지
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    • 제34권3호
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    • pp.287-296
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    • 2014
  • The physicochemical and functional traits for loin muscles of Hanwoo steers were compared by quality grade (QG). A total of 500 Hanwoo steers were slaughtered, their carcasses were categorized into four groups (QG 1++, 1+, 1, and 2), and the longissimus dorsi muscles were analyzed. QG 1++ group had the highest fat and lowest moisture content (p<0.05). QG 1++ showed higher $L^*$ and $b^*$ color values, higher cooking loss, and lower shear force values, compared with the other groups (p<0.05). The flavor, tenderness, juiciness, and preference scores by sensory evaluation were highly ranked for premium QG groups (1++ and 1+). Regarding the micro compounds, QG 1 and QG 2 had greater amounts of inosine monophosphate, and QG 2 had greater amounts of anserine, carnosine, and creatine, than QG 1++ (p<0.05). QG 1++ and 1+ had higher percentages of oleic acid (C18:1) than QG 2 (p<0.05). Within premium QG 1++ and 1+, the results of the nucleotides, free amino acids, dipeptides, and fatty acids did not show any distinctive differences. Hanwoo beef as determined by the current grading system was not significantly different in terms of functional components; the only significant difference was in intramuscular fat content.

한우 Fatty Acid Synthase (FASN) 유전자 반수체형의 후대검정우 육량 및 육질에 미치는 영향 (Effect of the Fatty Acid Synthase Gene for Beef Quantity Traits in Hanwoo Breeding Stock)

  • 김상욱;이준헌;김진호;원유석;김내수;김관석
    • Journal of Animal Science and Technology
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    • 제52권1호
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    • pp.9-16
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    • 2010
  • 본 연구는 지방산합성의 완성에 관여하는 소 FASN (Fatty acid synthase) 유전자내의 g.11280G>A (Silent), g.13125T>C (Exon24-His1390Tyr)와 g.17924G>A (Exon39-Thr2158Ala) 단일염기 변이들이 한우집단 925 두를 대상으로 도체형질과의 연관성 분석, 연관불균형의 정도 및 반수체형의 분석을 위하여 수행하였다. g.11280G>A 변이의 연관성분석에 있어서는 도체중, 등지방두께 및 육량지수에 유의성이 있는 것으로 관찰되었으며 g.17924G>A변이 역시 연관성분석에서 도체중에 유의성이 있는 것으로 나타났다(P<0.05). 하지만 g.13125T>C 변이는 한우집단에서 형질과의 유의적인 연관성을 관찰 할 수 없었다. 또한 g.11280G>A, g.13125T>C와 g.17924G>A 변이들에 대한 연관불균형(Linkage disequilibrium) 분석을 한 결과 g.11280G>A와 g.13125T>C 두변이에 대한 r-square=0.177로 나타났으며 D'=0.568 로 관측되었다. 또한 g.13125T>C와 g.17924G>A 두 변이들은 r-square = 0.207로 나타났으며 D'=0.607로 나타났으며 g.11280G>A와 g.17924G>A 변이들은 r-square = 0.101으로 나타났으며 D'=0.920으로 관찰되었다. 연관불균형 유의수준(r-square>0.1, D'>0.500)에 따라 계산된 반수체형 총 7개중 그 빈도가 0.05 이상인 중요한 반수체형은 4개(-GCG- [0.378], -ATG- [0.301], -GTA- [0.191], -ACG- [0.063])를 선별하여 연관성분석을 실시하였다. FASN 반수체형 1번(-GCG-), 반수체형 2번(-ATG-)와 반수체형 4번 (-ACG-)는 한우집단에서 형질과의 유의적인 연관성을 관찰 할 수 없었으나 FASN 반수체형 3번(-GTA-)은 도체중과 연관성을 보였다(P=0.0327). 염색체상 반수체형 -GTA-.구조를 가지지 않은 동형접합체(0-copies)인 개체의 도체중은 354.70+3.58로 나타났으며, 반수체형 -GTA- 구조를 하나만 가지는 이형접합체(1-copies) 347.63+4.84으로 관찰되었으며 0-copies인 동형접합체보다 현저히 낮은 도체중을 나타내었다. 또한 반수체형 -GTA- 구조를 가지는 동형접합체(2-copies)인 개체는 22두가 나타났으며 도체중은 351.01+4.18로 0-copies 인 동형접합체 보다 낮은 도체중을 나타내었다. 이상의 결과는 소 FASN 유전자내의 g.11280G>A, g. 13125T>C와 g.17924G>A 변이들은 육질 뿐만 아니라 육량에도 관여하는 것으로 사료되며, FASN 유전자내의 g.11280G>A와 g.17924G>A 두 개의 단일염기변이는 한우개량사업소의 씨숫소의 유전자 마커를 통한 도움 선발(marker assisted selection, MAS)에 이용 될 수 있을 것이며 일반농가에서도 송아지 생산을 위한 암소 선발에 활용될 수 있을 것으로 사료된다.

Identification of genetic polymorphisms in FABP3 and FABP4 and putative association with back fat thickness in Korean native cattle

  • Cho, Seo-Ae;Park, Tae-Sung;Yoon, Du-Hak;Cheong, Hyun-Sub;Namgoong, Sohg;Park, Byung-Lae;Lee, Hye-Won;Han, Chang-Soo;Kim, Eun-Mi;Cheong, Il-Cheong;Kim, Hee-Bal;Shin, Hyoung-Doo
    • BMB Reports
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    • 제41권1호
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    • pp.29-34
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    • 2008
  • The aim of this study was to determine whether single nucleotide polymorphisms (SNP) in the beef cattle adipocyte fatty-acid binding protein 3 and 4 (FABP3 and FABP4) genes are associated with carcass weight (CW) and back fat thickness (BF) of beef cattle. By direct DNA sequencing in 24 unrelated Korean native cattle, we identified 20 SNPs in FABP3 and FABP4. Among them, 10 polymorphic sites were selected for genotyping in our beef cattle. We performed SNP, haplotype and linkage disequilibrium studies on 419 Korean native cattle with the 10 SNPs in the FABP genes. Statistical analysis revealed that 220A>G (I74V) and 348+303T>C polymorphisms in FABP4 showed putative associations with BF traits (P=0.02 and 0.01, respectively). Our findings suggest that the polymorphisms in FABP4 may play a role in determining one of the important genetic factors that influence BF in beef cattle.

Determination of Point of Sale and Consumption for Hanwoo Beef Based on Quality Grade and Aging Time

  • Koh, Kyung Chul;Chung, Ku-Yong;Kim, Hyun-Seok;Kang, Se-Joo;Choi, Chang-Bon;Jo, Cheorun;Choe, Juhui
    • 한국축산식품학회지
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    • 제39권1호
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    • pp.139-150
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    • 2019
  • This study aimed to determine the suitable point of sale and consumption of different quality grade (QG) Hanwoo short loin during aging period, based on physicochemical, sensory, and microbiological quality. Short loins obtained from the carcasses of 13 Hanwoo steers and 2 bulls with 5 different QGs (1++, 1+, 1, 2, and 3) were analyzed over 28 d. QG and aging time had significant effect on water holding capacity, color, shear force, total volatile basic nitrogen (TVBN) content, and sensory traits. Higher QG groups generally exhibited a lower shear force, nucleotide content, and water holding capacity, and higher $L^*$, $a^*$, and $b^*$ values. Acceptable tenderness (shear force <5.4 kg) in QG 1++, 1+, 1, and 2 was achieved on days 7, 14, 16, and 18, respectively, and QG 3 showed a shear force of 6.8 kg, even after 28 d. Regardless of QG, TVBN content below threshold levels (20-30 mg%) was observed throughout the 28 d aging period, while total plate counts above 7 Log CFU/g were seen at 21 d. In conclusion, it is recommended that Hanwoo beef with QG 1++, 1+, and intermediate QG (1 and 2) should be sold or consumed between 7 and 21, 14 and 21, 16 and 21 d, respectively. Beef with QG 3 should be sold or consumed within 21 d, based on microbial growth, even though it has not achieved desirable tenderness. For this reason, an additional tenderizing process is recommended before this beef is ready for consumption.

Dairy Cows of High Genetic Merit for Yields of Milk, Fat and Protein - Review -

  • Norman, H.D.;Powell, R.L.
    • Asian-Australasian Journal of Animal Sciences
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    • 제12권8호
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    • pp.1316-1323
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    • 1999
  • Extensive emphasis on milk and milk fat yields with no diversion for beef performance increased the yield efficiency of North American dairy cattle. Heavy demand for North American genetics followed national strain comparison trials in Poland, and US and Canadian dairy cattle and germplasm still are an important source of genetics for many countries. Genetic improvement has accelerated in many countries because of the implementation of sampling programs for young bulls and improved evaluation procedures. Rapid access to information and more frequent calculation of genetic information also are having a positive impact on genetic improvement. Traits other than yield should be considered in a breeding program, but those traits mist have a reasonable opportunity for improvement and sufficient economic worth. Because of ever increasing efficiency, the world's milk supply comes from fewer cows each year. However, no decline in the rate of genetic improvement is apparent under current genetic practices; estimates of heritability are increasing, and a decline in yield efficiency is unlikely in the near future. As management improves, especially for subtropical conditions, many of the selection principles used in temperate climates will be adopted for more adverse environmental conditions.

Effect of Single Nucleotide Polymorphism of Endothelial Differentiation G-Protein Coupled Receptor 1 (EDG1) Gene on Marbling Score in Hanwoo

  • Shin, Sung-Chul;Chung, Eui-Ryong
    • 한국축산식품학회지
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    • 제32권6호
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    • pp.776-782
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    • 2012
  • Marbling (intramuscular fat) is the most economically important meat quality trait in Hanwoo (Korean cattle). The endothelial differentiation G-protein coupled receptor 1 (EDG1) gene, involved in blood vessel formation, is located within the genomic region of a quantitative trait locus (QTL) for marbling on bovine chromosome 3. Thus, the EDG1 gene can be considered as a positional and functional candidate gene for meat quality in beef cattle. This study aimed to identify single nucleotide polymorphisms (SNPs) in the EDG1 gene and to evaluate their associations with carcass traits in Hanwoo population. We have sequenced a fragment of 5'-UTR of the EDG1 gene and identified one SNP. Genotyping of the g.166A>G SNP marker was carried out using PCR-RFLP analysis in 309 Hanwoo steers in order to evaluate their association with carcass traits. The g.166A>G SNP marker showed a significant effect on the marbling score. Animals with the GG genotype had higher marbling score compared with AA and AG genotypes (p<0.05). This SNP marker also showed a significant additive effects for the marbling score (p<0.05). These results suggest that the EDG1 gene can be used as a molecular marker for DNA marker-assisted selection in order to increase the levels of the marbling score in Hanwoo.

Association of CAST Gene Polymorphisms with Carcass and Meat Quality Traits in Chinese Commercial Cattle Herds

  • Li, Jiao;Zhang, Lu-Pei;Gan, Qian-Fu;Li, Jun-Ya;Gao, Hui-Jiang;Yuan, Zheng-Rong;Gao, Xue;Chen, Jin-Bao;Xu, Shang-Zhong
    • Asian-Australasian Journal of Animal Sciences
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    • 제23권11호
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    • pp.1405-1411
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    • 2010
  • Calpastatin (CAST), an endogenous inhibitor of the calpains, plays an important role in post-mortem tenderization of meat. The objectives of this study were to investigate single nucleotide polymorphisms (SNPs) in the bovine CAST gene and association with carcass and meat quality traits. A total of 212 cattle from commercial herds were tested in this study including 2 pure introduced breeds, 4 cross populations, and 3 pure Chinese native breeds. Five SNPs were identified at position 2959 (A/G), 2870 (G/A), 3088 (C/T), 3029 (G/A) and 2857 (C/T) in the CAST gene (GenBank Accession No. AF159246). Allele frequencies of SNP2959 and SNP2870 were 0.701 (A) and 0.462 (A), respectively. A general linear model was used to evaluate the associations between the two markers and 7 traits. The results showed that both SNP2959 and SNP2870 were significantly (p<0.01) associated with the Warner-Bratzler shear force (WBSF), while they had no significant association with the other 6 traits in the whole population. However, in Chinese native pure breeds, only SNP2870 had significant association with WBSF (p<0.05). The simultaneous analysis of two-marker genotype effects indicated animals containing the A/G haplotype (A for SNP2959 and G for SNP2870) tended to have lower shear force than those containing the G/A haplotype, and, especially, animals homozygous for the A/G haplotype had approximately 2 kg lower shear force than those homozygous for the G/A haplotype (p<0.01). These results suggested that both markers may be effective for the marker-assisted selection of meat quality traits in Chinese commercial herds, especially SNP2870 which can be used for Chinese native cattle.

SNPHarvester를 활용한 주요 유전자 상호작용 효과 감명 (Identify Major Gene-Gene Interaction Effects Using SNPHarvester)

  • 이제영;김동철
    • Communications for Statistical Applications and Methods
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    • 제16권6호
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    • pp.915-923
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    • 2009
  • 광범위 유전자 연관(genome-wide association) 연구에서는 무수히 많은 유전자들 중에 인간의 질병에 관련된 유전자를 찾아왔다. 기존의 인간 질병에 관련된 유전자를 찾는 방법에서 이렇게 많은 유전자들 중에서 우수한 유전자를 찾는데 직접 이용할 시에는 계산이 복잡해지고 비용이 많이 들어가며 시간이 오래 걸린다는 단점이 생긴다. 따라서 이번 수많은 유전자들 중 주요 유전자 그룹을 찾는 방법으로 SNPHarvester가 개발되였다. 본 연구에서는 인간의 질병이 아닌 한우의 여러 경제형질에 관련된 우수 유전자를 SNPHarvester를 이용하여 17 개의 SNP들 중에서 우수한 유전자 그룹을 찾았고 의사결정나무(decision tree)를 이용하여 한우의 여러 경제형질을 높일 수 있는 SNP 그룹 내의 우수 유전자형도 함께 규명할 수 있었다.

National Genetic Evaluation (System) of Hanwoo (Korean Native Cattle)

  • Park, B.;Choi, T.;Kim, S.;Oh, S.H.
    • Asian-Australasian Journal of Animal Sciences
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    • 제26권2호
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    • pp.151-156
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    • 2013
  • Hanwoo (Also known as Korean native cattle; Bos taurus coreanae) have been used for transportation and farming for a long time in South Korea. It has been about 30 yrs since Hanwoo improvement began in earnest as beef cattle for meat yield. The purpose of this study was to determine the trend of improvement as well as to estimate genetic parameters of the traits being used for seedstock selection based on the data collected from the past. Hanwoo proven bulls in South Korea are currently selected through performance and progeny tests. National Hanwoo genetic evaluations are implemented with yearling weight (YW), carcass weight (CW), eye muscle area (EMA), backfat thickness (BF) and marbling score (MS). Yearling weights and MS are used for selecting young bulls, and EMA, BF, and MS are used for selecting proven bulls. One individual per testing room was used for performance tests, and five individuals per room for progeny tests. Individuals tested were not allowed to graze pasture, but there was enough space for them to move around in the testing room. Feeds including roughages and minerals were fed ad libitum, and concentrates were provided at the rate of about 1.8% of individual weight. Overall means of the traits were $352.8{\pm}38.56$ kg, $335.09{\pm}44.61$ kg, $77.85{\pm}8.838\;cm^2$, $8.6{\pm}3.7$ mm and $3.293{\pm}1.648$ for YW, CW, EMA, BF and MS. Heritabilities estimated in this study were 0.30, 0.30, 0.42, 0.50 and 0.63 in YW, CW, EMA, BF and MS, respectively, which are similar to results from previous research. Yearling weight was 315.54 kg in 1998, and had increased to 355.06 kg in 2011, resulting in about 40 kg of improvement over 13 yrs. YW and CW have improved remarkably over the past 15 yrs. Breeding values between 1996 and 2000 decreased or did not change much, but have moved in a desirable direction since 2001. These improvements correspond with the substantial increase in use of animal models since the late 1990s in Korea. Hanwoo testing programs have practically contributed to the improvement in aspects of quality and quantity. In sum, the current selection system is good enough to accommodate circumstances where fewer sires are used on many more cows. Although progeny tests take longer and cost more, they seem to be appropriate under the circumstances of the domestic market with its higher requirement for better meat quality. Consequently, accumulative data collection, genetic evaluation model development, revision of selection indices, as well as cooperation among farms, associations, National Agricultural Cooperative Federation, universities, research institutes, and government agencies must be applied to the Hanwoo selection program. All these efforts will assist the domestic market to secure a competitive position against imported beef under Free Trade Agreement trade system and will provide farmers with higher profits as well as the public with a higher quality of beef.