• 대한약침학회지
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• 제3권2호
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• pp.233-244
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• 2000

After observing a patient diagnosed with Progressive Muscular Dystropies from the August, 31, 2000 to the January, 2001, the following results are obtained. Method and Result: Under our assumption that the Korean Bee Venom Therapy is a good method to treat Progress Muscular Dystropies. Korean Bee Venom Therapy was applied on the following acupuncture points: BL23(Shinsu:腎兪), BL26(Guanyuanshu:關元兪), ST36(Chok-Samni:足三里), LI4(Hapkok:合谷), LV3(Taechung:太), SP10(Hyolhae:血海), SI9(Sojang-Kyonjong:貞). In addition CFC(Carthami Flos;紅花: and Cervi Pantotrichum Cornu;) Herbal-Acupuncture is also treated on the other acupuncture points. For herbal medication was given to the patient based on the Sasang Constitution, Taeyangin Ogapijangchuk-tang. Following these treatments in this case of Progressive Muscular Dystropies, the skeletal muscle functions made remarkable improvement. Conclusion: Based on the clinical results, traditional Korean Medical treatment is believed to be effective for treating Progressive Muscular Dystropies, and further studies should be conducted to provide more valuable information.

• 대한약침학회지
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• 제10권2호통권23호
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• pp.99-105
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• 2007

Objectives : It is the aim of this study to derive lurker studies evaluating the effectiveness of bee-venom phamacopuncture on malignant melanoma patients. We present a patient of malignant melanoma at right maxilla who survives over one year with stable disease (SD) by the treatment of Bee Venom Phamacopuncture (BVP). Methods : We followed the treatment and examination. We prescribed to the patient what to be taken 1.5cc BVP once a day. Picture series, Head series were followed-up and Neck computed tomography (CT) and positron emission tomography computed tomography (PET CT) were performed to evaluate the therapeutic efficacy. Results : The patient survives over one year and continued stable disease over 6 months. Picture series, Head series X-ray, neck CT and PET CT were shown no interval change. Conclusion : This case may give us the possibility that BVP offers potential benefits for patients with malignant melanoma.

• 대한약침학회지
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• 제12권2호
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• pp.85-90
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• 2009

Objectives : Sweet bee venom(SBV) is pure melittin, the main component of bee venom, made by removing another components through gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis. In the using SBV, 2 patients were experienced anaphylaxis by SBV. So, we reported the process of anaphylaxis and consider these situation. Methods : We observed 2 patients suffered from anaphylaxis after treated with SBV in the Korean Medical Hospital, Sangji University. Results : Though SBV was removed allergen from bee venom, it is not possible to complete prevention of anaphylactic shock in the clinics. So, Korean medical doctor using BV or SBV must be prepare the system consider a countermeasure by anaphylaxis.

• Journal of Acupuncture Research
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• 제36권4호
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• pp.220-229
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• 2019

Background: The therapeutic potential of Bee Venom Pharmacopuncture (BVP) on acute ischemic cerebral infraction was determined in mice in vivo and in vitro. Methods: Analysis of acute ischemic cerebral infraction was performed using 7 week old male ICR mice (n = 20) and microglial BV-2 cells. Bee venom ($5{\mu}g/kg$) was injected into the caudal vein of middle cerebral artery occlusion (MCAo) mice (1 hour after reperfusion, 3 hours after MCAo probe insertion), and also used to treat LPS-stimulated microglial BV-2 cells (1, 2, $5{\mu}g/mL$). Markers of inflammation were monitored. Results: NO declined statistically significantly in BVP treated MCAo mice compared to the untreated MCAo group (p < 0.05). Compared to the MCAo group, the BVP-treated MCAo group showed a decreased production volume of malondialdehyde, but an increased glutathione/oxidized glutathione ratio. Compared to the untreated MCAo group, the BVP treated MCAo group showed a statistically significant decline in TNF and $IL-1{\beta}$ levels (p < 0.05). BVP inhibited the levels of p65, p50, $p-I{\kappa}B-{\alpha}$, and levels of p-ERK1/2, p-JNK2, p-P38 declined. Conclusion: BVP is effective at dampening the inflammatory response in vivo and in vitro and may supplement rt-PA treatment.

• 한국어병학회지
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• 제24권2호
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• pp.113-120
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• 2011

본 연구에서는 양식 넙치의 주요 어병세균인 E. tarda, V. ichthyoenteri와 S. iniae에 대한 국내산 꿀벌로부터 분리한 봉독의 항균활성을 검색하였다. 그 결과 봉독은 폐사 납치로부터 분리한 그람음성균인 E. tarda, V. ichthyoenteri와 양성균인 S. iniae 모두에서 강한 항균 활성을 나타내는 것으로 확인되었다. 봉독은 E. tarda와 V. ichthyoenteri에 대하여 최소발육저지 농도는 각각 17.6 ${\mu}g$/ml, 1.76 ${\mu}g$/ml 였으며, 최소살균 농도는 34.6 ${\mu}g$/ml, 6.8 ${\mu}g$/ml, 항균력 지속시간은 5시간, 6시간으로 측정되었다. 또한 그람 양성균인 S. iniae에 대한 최소발육저지농도는 3.49 ${\mu}g$/ml, 최소살균농도는 11 ${\mu}g$/ml였으며, 항균력지속시간은 7시간으로 봉독은 매우 강한 증식 및 살균 억제력을 보였다. 뿐만 아니라, 다양한 pH 조건하에서도 향균활성이 유지되는 것으로 확인되었다.

• 생명과학회지
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• 제31권2호
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• pp.144-148
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• 2021

본 연구의 목적은 항염, 항균 등 다양한 기능성을 갖는 서양종꿀벌(Apis mellifera L.)의 일벌독에서 분리한 봉독의 활성아민 성분과 함량을 분석하고 그 분석법을 검토하였다. 본 연구에서는 Ultra Performance Liquid Chromatography에 Halo C18컬럼과, acetonitrile 및 증류수를 이동상으로 사용하여 분석시간 13분 이내에서 봉독의 cadaverine에 대한 성분 분석법을 개발하였고, 분석법 밸리데이션을 통하여 특이성, 정확성, 정밀성을 갖춘 분석법임이 확인되었다. 봉독의 cadaverine의 직선성은 R2=0.99 이상으로 측정되어 양호하게 분석되었고, 검출한계는 0.3 ㎍/ml, 정량한계는 0.3 ㎍/ml으로 나타났으며, 회수율은 97.6~99.1%로 나타났다. Cadaverine의 일내 정밀도는 상대표준편차(RSD) 값이 0.25~0.44%였으며, 일간 정밀도는 0.25~1.25%로 상대표준편차 값이 5% 이내의 우수한 정밀성을 갖는 것으로 확인되었다. 이러한 분석법을 통해 지금까지 봉독에서는 보고된 바 없는 활성아민 cadaverine이 평균 1.10±0.05 mg/g 함유된 것으로 확인되었다. 따라서 본 연구는 봉독에서 cadaverine 함량과 cadaverine 분석법에 대한 직선성, 검출한계, 정량한계 및 회수율을 측정한 결과, 모두 만족스러운 결과를 나타냈으며, 봉독의 cadaverine에 대한 기초자료와 생리활성에 연구로 사용될 수 있을 것으로 기대된다.

• Journal of Acupuncture Research
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• 제20권5호
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• pp.50-62
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• 2003

Objective: Bee Venom(BV) has been used for various kinds of inflammatory or painful conditions in Oriental Medicine clinics, and there publishes reports on its therapeutic effects and the probable mechanism of those therapeutic effects, where CDs and cytokines plays important role. This study investigated the influences of bee venom on the expressions of CDs and cytokines of HMC cell line Methods: In this study we analysed the expression profile of HMC cell line treated with BV of 10-2ug/ml in relation to that of HMC cell line treated with vehicle by way of CD/cytokine microarray hybridization with 342 genes on it. Results: There were no upregulated genes by more than 3 fold, while there showed some downregulated genes by less than 1/3 fold as follows: colony stimulating factor 2, CD122, IL-7, CD112, TNF-alpha, CD138, CD166, TGFbetaR2, CD42b, CD62L, CD111, interleukin 10 receptor alpha, colony stimulating factor 1(macrophage), CD38 antigen(p45), CD121a, CD33 antigen(gp67), colony stimulating factor 1 receptor, B cell linker protein (SLP65) mRNA, CD94, alanyl(membrane) aminopeptidase, immunoglobulin(CD79A) binding protein 1, CD205, CD241, CD207, CDw121b, integrin alpha L(CD11a), integrin beta 1(CD29), CD91, CD42b. Conclusions: Bee venom treatment induced downregulation of some CDs or cytokines including $TNF-{\alpha}$. IL-1R with its possible implication in an antiinflammatory action of BV. Further research on expression profile changes induced by BV treatment is expected.

• Journal of Acupuncture Research
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• 제33권2호
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• pp.173-180
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• 2016

Objectives : To treat the progression of fibroproliferative disease that affects the flexion contracture of the fingers for patients with Dupuytren's contracture, the purpose of this study is to report a case of a patient with Dupuytren's contracture after complex Korean medical treatment. Methods : A patient was treated with acupuncture, moxibustion and bee venom pharmacopuncture on their left palmar aponeurosis. Six rounds of acupuncture and moxibustion were administered from November 30, 2015 through to January 2, 2016. Three rounds of bee venom pharmacopuncture was administered from December 14, 2015 through to January 2, 2016. The degree of flexion contracture and the Tubiana's stage were measured to evaluate the clinical improvement. Results : After 30 treatment sessions the flexion contracture degrees of the 4th finger's metacarpophalangeal joint and proximal interphalangeal joint improved as much as $25^{\circ}$, $15^{\circ}$, respectively. And the flexion contracture degrees of the 5th finger's metacarpophalangeal joint, proximal interphalangeal joint and distal interphalangeal joint improved as much as $15^{\circ}$, $10^{\circ}$, $5^{\circ}$, respectively. The Tubiana's stage of each finger decreased from 4 to 3. Conclusion : This study suggests that acupuncture, moxibustion and bee venom pharmacopuncture could be effective for patients with Dupuytren's contracture.

• 대한약침학회지
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• 제17권4호
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• pp.15-21
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• 2014

Objectives: The purpose of this study was to investigate the current trends in research on pharmacopuncture in Korea since 2007. Methods: A literature review was performed by using the search engines 'Science and Technology Society Village', 'Korean Studies Information Service System', 'National Discovery for Science Leaders', and 'Oriental Medicine Advanced Searching Integrated System' in Korea from January 2007 to December 2013. Searched key words were 'pharmacopuncture', 'herbal acupuncture', 'aqua-acupuncture', and 'bee venom'. Finally, we selected 457 papers, including Korean experimental studies and clinical studies. Selected papers were classified according to year of publication, type of pharmacopuncture, disease & topic, research type and the publishing journal. Results: One hundred fifty pharmacopunctures were studied in 457 papers. Single compound pharmacopuncture was the most studied pharmacopuncture in experimental studies while animal-based pharmacopuncture was the most studied pharmacopuncture in clinical studies. Bee venom placed first among the various pharmacopunctures, followed by placenta, sweet bee venom, mountain-ginseng, and anti-inflammatory pharmacopunctures. Experimental research on pharmacopuncture has fallen since 2007 when 55 papers were published. However, clinical research has been increasing steadily. In clinical studies, case reports were numerous than randomized clinical trials (RCTs). Musculoskeletal diseases were the most frequently-treated diseases in studies on pharmacopuncture; among the musculoskeletal diseases, rheumatoid arthritis was the most frequently-treated disease in experimental studies and low back pain was the most frequently-treated condition in clinical studies. Since 2007, 45 different journals have published studies on pharmacopuncture, with the Journal of the Korean Acupuncture and Moxibustion Medicine Society having the largest number of papers on pharmacopuncture and the Journal of Pharmacopuncture the second largest number. Conclusion: The trends in research on pharmacopuncture published in studies from 2007 to 2013 were similar to those in studies published before 2006. Many studies on pharmacopuncture focused on bee venom and musculoskeletal diseases. Additional studies on diverse types of and indications for pharmacopuncture are needed.

• 대한추나의학회지
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• 제4권1호
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• pp.17-28
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• 2003

관절염에 대한 스테로이드의 관절강내 주사는 전신적인 부작용 등의 위험성이 적으면서 간편하고 경제적이며 비교적 양호한 항염효과를 얻을 수 있어 단일관절의 RA 또는 퇴행성 관절염에서 활액막의 염증증상이 있을 때 또는 보존적 치료에 반응하지 않는 경우 비교적 많이 사용된다. 하지만 이러한 스테로이드요법은 여러가지 만성 질환에서 과도한 염증이나 면역반응 자체를 치료하는 것이 아니라 부수되는 이차적 증상만을 완화시켜 주기 때문에 장기간의 스테로이드제 투여는 절병의 경과를 교정 하거나 변화시키지 못하고 예후에 악영향을 미칠 수도 있다. 또한 심각한 여러 부작용들을 야기할 수도 있다. 저자는 스테로이드 관절강내 주사에 호전되지 않은 부종과 통증을 주소로 하는 슬관절염 환자에 대해, 피하 봉독요법으로 양호한 효과를 얻었으며, 또한 기존에 알려져 있는 것처럼 당뇨병환자에게서 봉독요법이 혈당치를 지속적으로 높인다는 주장에 대해 다른 결과를 경험하였으므로 문헌조사와 함께 보고하는 바이다.