• Clinical and Experimental Reproductive Medicine
• /
• v.29 no.2
• /
• pp.139-147
• /
• 2002

Objective : This study was to establish a reproducible differentiation system from the parthenogenetic mouse embryonic stem (P-mES02) cells into functional cardiomyocytes like as in vitro fertilization mouse embryonic stem (mES01) cells. Materials and Methods: To induce differentiation, P-mES02 cells were dissociated and aggregated in suspension culture environment for embryoid body (EB) formation. For differentiation into cardiomyocytes, day 4 EBs were treated with 0.75% dimethyl sulfoxide (DMSO) for another 4 days (4-/4+) and then were plated onto gelatin-coated dish. Cultured cells were observed daily using an inverted light microscope to determine the day of contraction onset and total duration of continuous contractile activity for each contracting focus. This frequency was compared with the results of DMSO not treated P-mES02 group (4-/4-) and mES01 groups (4-/4+ or 4-/4-). For confirm the generation of cardiomyocytes, beating cell masses were treated with trypsin-EDTA, dispersed cells were plated onto glass coverslips and incubated for 48 h. Attached cells were fixed using 4% paraformaldehyde and incubated with specific antibodies (Abs) to detect cardiomyocytes (anti-sarcomeric ? -actinin Ab, 1 : 100; anti-cardiac troponin I Ab, 1 : 2000) for 1 h. And the cells were finally treated with FITC or TRITC labelled 2nd Abs, respectively, then they were examined under fluorescence microscopy. Results: Rhythmically contracting areas in mES01 or P-mES02 cells were firstly appeared at 9 or 10 days after EBs plating, respectively. The highest cumulative frequency of beating EBs was not different in both treatment groups (mES01 and P-mES02, 4-/4+) with the results of 61.3 % at 13 days and 69.8% at 15 days, respectively. Also, the contracting duration of individual beating EBs was different from minimal 7 days to maximal 53 days. However, DMSO not treated groups (mES01 and P-mES02, 4-/4-) also had contracting characteristics although their frequency was a few compared to those of DMSO treated groups (6.0% and 4.0%). Cells recovered from the spontaneously contracting areas within EBs in both treated groups were stained positively with muscle specific anti-sarcomeric ? -actinin Ab and cardiac specific anti-cardiac troponin I Ab. Conclusion: This study demonstrated that the P-mES02 cell-derived cardiomyocytes displayed similarly structural properties to mES01 cell-derived cardiomyocytes and that the DMSO treatment enhanced the cardiomyocytes differentiation in vitro.

• Ocean and Polar Research
• /
• v.40 no.3
• /
• pp.161-167
• /
• 2018

We examined the effect of salinity and water temperature on hatching and survival rates of fertilized eggs of hybrid grouper (Epinephelus fuscoguttatus ♀ ${\times}$ E. lanceolatus ♂) at different developmental stages, determining optimal conditions for their long-distance transportation. Deformities and hatching rates of fertilized grouper eggs were observed at salinities of 24, 27, 30, 33, 36, and 39 psu. The optimal salinity was determined to be 36 psu, with a survival rate of $70.0{\pm}2.0%$. Transportation experiments at 36 psu were conducted at water temperatures of 21, 24, 27, and $30^{\circ}C$, different developmental stages such as morula, 5-myomere, and tail beating for hatching and survival rates. The optimal water temperature and developmental stage for transporatation were $30^{\circ}C$ and tail beating stage and those hatching rates were $50.6{\pm}1.9%$ and $86.3{\pm}1.3%$, respectively. At $21^{\circ}C$, the survival rate by transportation water temperature was highest ($73.1{\pm}10.6%$), but the hatching rate ($17.1{\pm}3.1%$) was lowest. Therefore, the hybrid grouper fertilized eggs (E. fuscoguttatus ♀ ${\times}$ E. lanceolatus ♂) can be most efficiently produced under long-distance transportation conditions during the tail beating stage and at a water temperature of $30^{\circ}C$.

• Reproductive and Developmental Biology
• /
• v.30 no.1
• /
• pp.47-52
• /
• 2006

This study was conducted to examine whether the parthenogenetic mouse embryonic stem (P-mES) cells can differentiate into functional cardiomyocytes in vitro similar to (mES) cells. p-mES04 and IVF-derived mES03 cells were cultured by suspension culture for 4 days. The formed embryoid bodies (EBs) were treated with 0.75% dimethyl-sulfoxide (DMSO) for further 4 days (4-/4+), and then plated onto gelatin coated culture dish. The appearance of contracting cardiomyocytes from the P-mES04 and mES03 cells was examined for 30 days. The highest cumulative frequency was detected at days 13 (69.83%) and 22 (61.3%), respectively. By immunocytochemistry, beating P-mES04 cells were positively stained with muscle specific anti-sarcomeric a-actinin Ab and cardiac specific anti-cardiac troponin I Ab similar to contracted mES03 cells. When the expression of cardiac muscle-specific genes was analyzed by RT-PCR, beating P-mES04 cells were expressed cardiac specific L-type calcium channel, a1C, cardiac myosin heavy chain a, cardiac muscle heavy polypeptide $7{\beta}$, GATA binding protein 4 and atrial natriuretic factor, but not expressed skeletal muscle specific L-type calcium channel, a1S, which was similar to male adult heart cells and mES03-derived beating cardiomyocytes. The result demonstrates that the P-mES cells can be used as an alternative for the study on the characteristic analysis of in vitro cardiomyocyte differentiation from the ES cells.

• Korean Journal of Ichthyology
• /
• v.33 no.1
• /
• pp.8-14
• /
• 2021

Spawning behavior of the endangered Korean fish, Microphysogobio koreensis, was investigated in the Seomjingang (river), Imsil-gun, South Korea, during the spawning season, April to May 2012. The mating system of M. koreensis, a broadcast spawner, was a primitive spawning mode, and involved one male and one female, unlike group spawning fishes. Spawning behavior of M. koreensis in the wild were observed in eight patterns as resting, male chase, body beating, parallel swim, female withdrawal, male competition, spawning and not guard while spawning behavior in the glass tank were verified in six patterns as resting, male chase, body beating, parallel swim, spawning and not guard. In particular, a behavioral attempt of the pre-spawning stage showed more frequently in the wild than in the glass tank. We assume that difference of spawning behavior might be implication on behavioral restrictions in small and narrow indoor glass tank.

• Journal of Naturopathy
• /
• v.7 no.1
• /
• pp.1-9
• /
• 2018

The purpose of this study is to statistically investigate the changes in mean differences of the vascular age types and index, heart beating, vessel elasticity, diastolic reserves contents measured by the fingertip plethysmogram before and after the subjects (49 people) visit the Gotjawal forest for two hours. While there is no significant change in the mean differences of vascular age types and index, heart beating, diastolic reserves contents in the control group before and after the visit, the differences in the test groups gets higher than the control group and shows a significant difference. Moreover, the variance analyses of the mean values of the contents shows a significant change after the visit. There is no significant change in the values of vessel elasticity changes between the groups after the visit, but the change of values is significant in the within group, and it is not significant between group by variance analysis. The differences of the heart beating was insignificant in the within group, but significant between group after the visit. In conclusion this study proves the effects of forest bathing, because the HRV index significantly improved after the bathing. Therefore the b-c-d-e/a ratio may be useful for evaluation of HRV.

• Archives of Pharmacal Research
• /
• v.7 no.1
• /
• pp.61-62
• /
• 1984

The toxicity of water extracts from intestine parts (digestive tract respiratory tree) of Korean Stichopus japonicus was determined using mouse units and more purified substance decreases the amplitude of contraction of guinia pig atria in vitro; showes negative chronotropic and ionotropic effects in the spontaneously beating guinea pig atria.

• The Korean Journal of Soil Zoology
• /
• v.1 no.2
• /
• pp.110-119
• /
• 1996

The authors investigated the spider fauna of Mt. Yebongsan from May 1994 to Aug. 1996. In this paper, 265 species from 21 genera, and 28 families were identified and compared with those from islands geographical with differing i.e., Is. Paikryung-do, Is. Chin-do, and Is. Ulrung-do distribution and climate. Spiders from 45 palaearctic regions(3.69%), 13 holarctic regions(1.06%), 4 oriental regions(0.32%) and 5 cosmopolitans(0.41%) are identified. Various methods were used for collecting specimens during the period including pitfall, beating, sweeping and looking up-down.

• Proceedings of the Korean Society of Tribologists and Lubrication Engineers Conference
• /
• 1989.11a
• /
• pp.94-99
• /
• 1989

This paper analyzes file stiffness, damping coefficient, friction force and flow coefficient of externally pressurized oil journal beating, including the effect of journal rotation according to the Sommerfeld number. This paper assumed that the oil in the whole pocket has constant pressure, and that the oil in the whole bearing region has constant viscosity, temperature and density. Reynolds equation is derived from Nuvier - Stokes equation and continuity equation. And solved bearing pressure by ADI method for whole bearing region and fitted with out flow rate of pocket region. The model for numerical simulation is hydro - static oil journal bearing for high-speed, high-accuracy lathe spindle.

• Journal of the Korean Society for Precision Engineering
• /
• v.17 no.6
• /
• pp.25-31
• /
• 2000

"모아레(MOIRE)"란 말은 처음으로 프랑스인들에 의해 불려진 단어로써 고대 중국에서 수입된 비단 위에 새겨진 물결무늬(wave pattern)를 일컫는 말이다. 이렇게 불리어지던 모아레는 요즈음에 들어서서 두 개 이상의 주기적인 패턴(periodic pattern)이 겹쳐질 때 만들어지는 간섭무늬 (interference fringe)를 지칭하는 말로써 다양한 분야에서 매우 광범위하게 사용되어 지고 있다.(중략)

• Journal of the Korean Professional Engineers Association
• /
• v.34 no.1
• /
• pp.59-65
• /
• 2001

Imported China Jades are mostly talc and serpentine groups, and that's occured troubles for our health and life of living organism. Serpentine is a group of common rock-forming minerals having the formula:(Mg$.$Fe)$^3$Si$_2$O$\_$5/(OH)$_4$. Sometime, simulated by nephrite or jade and translucent varieties we used for ornamental and decorative purposes. But it's forbid by heating materials for sauna and heating mats etc. that reason are it's occured asbestos dusts(chrysotile dusts) easily by the beating operations.