• Environmental Engineering Research
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• v.14 no.1
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• pp.63-67
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• 2009

Escherichia coli K-12 (E. coli K-12) is a representative indicator globally used for distinguishing and monitoring dynamic fates of pathogenic microorganisms in the environment. This study investigated how to most critically quantify lacZ ($\beta$-galactosidase) gene in E. coli K-12 by two different real-time polymerase chain reaction (real-time PCR) in association with three different DNA extraction practices. Three DNA extractions, i.e., sodium dodecyl sulfate (SDS)/proteinase K, magnetic beads and guanidium thiocyanate (GTC)/silica matrix were each compared for extracting total genomic DNA from E. coli K-12. Among them, GTC/silica matrix and magnetic beads beating similarly worked out to have the highest (22-23 ng/${\mu}L$) concentration of DNA extracted, but employing SDS/proteinase K had the lowest (10 ng/${\mu}L$) concentration of DNA retrieved. There were no significant differences in the quantification of the copy numbers of lacZ gene between SYBR Green I qPCR and QProbe-qPCR. However, SYBR Green I qPCR obtained somewhat higher copy number as $1{\times}10^8$ copies. It was decided that GTC/silica matrix extraction or magnetic beads beating in combination with SYBR Green I qPCR can be preferably applied for more effectively quantifying specific gene from a pure culture of microorganism.

• Journal of Korea Multimedia Society
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• v.14 no.11
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• pp.1507-1515
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• 2011

As the first step in research for sound synthesis of Pyungjong, timbre was analyzed by FFT analysis. There was no significant difference in the pitch of scale between modern Pyungjong and western twelve-tone equal temperament scale. The timbre of Pyunjong chagnes over time. When striking Pyunjong, the noises to be generated across all frequencies will disappear in a short period of time, and a unique partial only is left. Then it disappear from the partial of high frequency components in order, and two partials remain at release time. At this time, the beating is occurring by the frequency of the remaining partial. Pyungjong used in this study is relatively a recently manufactured Pyungjong, and for original sound synthesis of Pyungjong, a study of ancient Pyungjong's timbre should be additionally conducted later.

• Tribology and Lubricants
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• v.14 no.1
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• pp.7-13
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• 1998

The static characteristics of a five-pad tilting pad journal bearing of load on pad (LOP) type have been investigated experimentally under the different values of bearing load and shaft speed. The diameter and length of the bearing are 300.91 mrn and 149.8 mm, reslx;ctively. Circumferential distribution of the film pressure, film thickness, journal surface temperature and beating surface temperature are measured. A noticeable inlet pressure rise is observed at the entrance of each pad, especially the bottom pad. The inlet pressure is increased by the increase of shaft speed as well as bearing load. In the five-pad tilting pad joumal bearing of LOP type, almost all of beating load is being carried only by the bottom pad. The maximum bearing surface temperature is observed at near the minimum film thickness. It is observed that the metal temperature of the mid-plane is higher than that of the edge at the inlet region, while the metal temperature of the edge is higher than that of the mid-plane at the outlet region.

• Research in Community and Public Health Nursing
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• v.12 no.2
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• pp.482-490
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• 2001

Purpose I investigated how beliefs in punishment have influence on child abuse. Also, I investigated how socio-demographic variables of parents' have influences on child abuse by parents. Sampling was conveniently and total 160 parents replied to the study. Data analysis was done by descriptive statistics, Pearson's correlation using SPSSWIN. The results were as follows. Mild child abuse: 'Throwing the object at me(21.1%)', 'Clutching, and pushing (21.0%)', 'Slapping on the cheek (19.1 %), Severe child abuse: 'Kicking, pounding and biting(4.1%)', 'Rod, stick, belt, broom beating using a various objects such as(51.7%)', 'Beating all over the body(6.8%)'. Very severe child abuse: 'Burning with cigarette (0%)', 'Threatening me with a knife, a hammer, a naxe, a gimlet the objects such as(0.7%)', 'Injuring me with the objects such as (0%)', 'Hospitalized by belting(0%)'. Correlations of major variables were as follows. Physical child abuse by elementary student's parents has significantly positive correlation with beliefs in punishment(r= .244 p= .003) and has significantly negative correlation with parent's age(r=-.273, p=.001). But physical child abuse has not significant correlations with family income, education. Conclusion: Beliefs in punishment of parents have influences on child abuse. To prevent child abuse, effective parenting program development is required.

• 연구논문집
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• s.29
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• pp.111-121
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• 1999

Weft insertion and Beating mechanism which weaves fabric with completion the pattern of warp yarn and weft yarn in the high speed rapier loom is driven by cam operating method. This mechanism has 4 cams on the coaxial line for driving. These cams form a pair by two. and perform a conjugate motion with each other. By this reason, it is necessary for two pairs to realize the synchronized motion through exact and optimal design. From this viewpoint, to analysis the whole cams' movement and to realize the high speed of rapier loom, I tried to develop the computer aided simulation program. In this study, I would like to introduce the simulation program about above-mentioned 4 kinds of cam mechanism which can display the profiles of 2 pairs of cam mechanism not to speak of mutual relationships of their movement on the monitor.

• Journal of Advanced Marine Engineering and Technology
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• v.28 no.2
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• pp.382-392
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• 2004

The usage of constant velocity (CV) joint is effective for motorboats on gliding regime of the motion. During transition on the gliding when angle of the CV differs from null on driving and driven composite shafts there are moments of the second order. Excitation of oscillations of the second order moments occurs when driving shafts transmits a variable torque. which generates through CV joint a lateral moment acting on the bearing. As a result of oscillations from a resonating harmonic of a shafting the harmonic with the greater or periodically varying amplitude for power condition trough transferring to nominal power 144kW. Beating conditions coincide with third mode having frequency 45.486 Hz. In that case there is high increasing of the equivalent stresses. The forming of the stiffness of the composite material is concerned to use most orientation of the layer angle in the range of $\pm$60 degrees relatively of shaft axis. Application of that angles for layer orientation gives possibility to avoid high disturbance of the shafting for motorboat transition regime.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2012.10a
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• pp.1009-1011
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• 2012

In this paper, a wavelength tunable light source based on mutually injected locking with two F-P LDs, has been constructed and then analyzed for wavelength shift and RIN (Relative Intensity noise) according to temperature. We have measured maximum about 2 nm for the wavelength shift and minimum -110dB/hz for the RIN. Also, the RIN and beating noise in eye patterns are increased by changing temperature high.

• Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
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• 1999.11b
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• pp.172-177
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• 1999

Effect of fiber treatment with cellulase (Liftase A40), and with two refining methods (Valley beating and impact refining) on wood fiber and handsheet properties were investigated at three refining levels (300, 400, and 500ml) for two furnishes (NBKP and LBKP). Part of the treated furnishes were classified by 150 mesh screen into fine-free fiber, and fines. Fiber length analysis, WRV, zero-span strength, and other handsheet mechanical properties were compared. The study showed that Liftase A40 lowered the zero-span and the folding endurance of both furnishes (NBKP much more and LBKP much less). Pretreatment with Liftase A 40 followed by refining significantly lowered the fiber length and refining energy to reach to the target freeness. Impact refining, which is done by hitting the fibers vertically with rod at 20% solid content, kept the fiber length increased WRV, and improved handsheet mechanical properties much more than valley beating. Properties of fines from different sources were compared in detail in the study.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.35 no.2
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• pp.58-64
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• 2003

Because of its tendency of making strong Hoc on the fiber surface with fines, Meogujaengi has not been valued as a material of Hanji. As an attempt to manufacture high value-added products using the material made from Jumchihanji, this study performed morphological classification and chemical component analysis and selection of pulping of Meogujaengi method. As a result, it can be concluded as follows, 1. Meogujaengi is assumed to be a local variety of Broussonetia karinoki and its outward appearance is distinguished from Broussonetia kazinoki. 2. The bast fiber of Meogujaengi is longer and thinner than that of Broussonetia papyrifera or Broussonetia kazinoki. However, because of the coarse linear of fiber tissue, there are many clusters. 3. The cluster phenomenon of Meogujaengi is nothing to do with its chemical components. Although the contents of its chemical components are different from those of Broussonetia kazinoki, no component was found that obstructs pulping. 4. The pretreatment for suppressing the occurrence of clusters of Meogujaengi was effective, and it was necessary to do secondary beating using hollander beater after beating mixed with PAM using knife beater.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.43 no.2
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• pp.31-39
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• 2011

The applicability of arrowroots as materials for paper manufacture was investigated by evaluating the characteristics of arrowroots pulping and pulps, and the surface characteristics of paper made of arrowroots. The results is as follows. The yield of grounded pulp, alkaline pulp, grounded bleach pulp, and alkaline bleach pulp are 98.0%, 37.7%, 84.8%, and 32.5%, respectively. The extraction components in arrowroot's fibers was shown high in 1%-NaOH extraction. The amount of holocellulose and lignin were largely decreased, but a slight decrease was shown in the bleaching processes. Many shivers were shown in the grounded fibers. The amount of shivers was decreased and the single fibers were increased during the process of bleaching. The alkaline pulp and the alkaline bleach pulp showed high in the beating time and freeness than the grounded pulp and grounded bleach pulp. The conditions of sheet surface according to beating time is effected largely by the conditions of original pulp.