• 제목/요약/키워드: Batch Experiment

검색결과 452건 처리시간 0.024초

백색부후균을 이용한 분산염료용액의 색 제거 (Color Removal from Disperse Dye Solution Using White Rot Fungi)

  • 이현욱;손동찬;임동준
    • 한국염색가공학회지
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    • 제12권1호
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    • pp.32-43
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    • 2000
  • Batch culture system and continuous culture systems were used to investigate the removal of disperse dye using white rot fungi. White rot fungi used in the study were Coriolus hirsutus IFO 4917, Lenzites betulina IFO 6266, Coriolus versicolor IFO 30340 and Phanerochaete chrysosporium IFO 31249. The results of the batch culture experiment showed that white rot fungi used in this study had excellent dye removal abilities. Phnerochete chrysosporium IFO 31249 was especially effective on the removal of disperse dyes. And continuous treatment of disperse red 60 was studied under two type of reactor using Phanerochaete chrysosporium IFO 31249. The removal efficiency of disperse red 60 for immobilized Phanerochaete chrysosporium IFO 31249 in continuous reactor with vertical matrix was increased 1.3 fold in $1.4\;hr^{-1}$ dilution rate when compared with continuous reactor without vertical matrix.

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High Density Cell Culture of Mucor sp. KCTC 8405P for Production of ${\gamma}-Linolenic$ Acid in Fed-Batch Culture

  • Park, Jong-Hyun;Shin, Hyun-Kyung
    • Journal of Microbiology and Biotechnology
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    • 제1권2호
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    • pp.126-129
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    • 1991
  • In order to produce ${\gamma}-linolenic$ acid by Mucor sp. KCTC 8405P. the fungus was cultivated in fed-batch culture with two phases. i.e., growth in yeast-like form and induction to hyphal growth by pH shift of the culture medium during cultivation. The synchronous growth of the fungus into the appropriate sizes was important for the high density cell culture of this dimorphic fungus. Dissolved oxygen concentration in the medium did not affect degree of unsaturation of fatty acids and ${\gamma}-linolenic$ acid content. Under the culture conditions applied in this experiment. the fungus was found to produce 100 g/l dry mycelia containing 40% of the lipids, where ${\gamma}-linolenic$ acid comprised about 9% of the total extractable fatty acids.

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ALC(Autoclaved Light-weight Concrete)를 이용한 생물학적 반응벽체에 관한 연구

  • 박근민;이재영;오병택;최상일
    • 한국지하수토양환경학회:학술대회논문집
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    • 한국지하수토양환경학회 2006년도 총회 및 춘계학술발표회
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    • pp.402-406
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    • 2006
  • The physical and chemical characteristics of ALC were analyzed and showed 2.2 of specific gravity and 9.05 of pH. The results of leaching tests with standard method for soil and waste indicated heavy metals(Cu, Cd, Pb, $Cr^{6+}$) were under maximum concentration level. The anaerobic digestion sludge was attached in the surface of ALC within 90 hours. As the results of batch test, pH of the ALC and Bio-ALC were decreased from initial pH of ALC to 8.7 and 7.8 respectively Also, the concentration of heavy metals was rapidly eliminated in the solution with the batch test. The result of column experiment indicates that the removal efficiency of ALC was showed 66% of T-P, 60% of T-N, and 67% of CODcr. Also, removal efficiency of Bio-ALC was slightly higher than that of ALC in T-N (64%) and CODcr (74%).

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자외선 처리시의 의약품류의 분해도에 관한 연구 (A Study on the Degradability of Pharmaceuticals during UV Treatment)

  • 김일호
    • 한국물환경학회지
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    • 제28권6호
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    • pp.902-910
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    • 2012
  • The photodegradation characteristics of 30 pharmaceuticals were investigated by batch experiments using Ultraviolet (UV) reactor. The investigated pharmaceuticals include antibiotics, analgesics and antiarrhythmic agents etc. Tested water was prepared by simultaneously spiking 30 pharmaceuticals into pure water, and each experiment was conducted using 3 types of UV lamps. As a result, batch experiments showed that reactions of all the investigated pharmaceuticals followed pseudo-first order reaction regardless of the applied UV lamps. Among the pharmaceuticals, Cyclophosphamide, 2-Quinoxaline carboxylic acid and Clarithromycin proved to be the most UV-resistant compounds. Contrarily, Ceftiofur, Diclofenac and Ketoprofen were easily degraded by all the UV lamps. Dissolved organic carbon (DOC) concentration hardly changed although the concentration of the pharmaceuticals concentration gradually decreased with time, indicating that the degradation of parent pharmaceuticals may produce their intermediates during UV treatment.

알루미늄의 부식으로 발생한 알루미늄 이온에 의한 인 제거 (Phosphorus Removal by Aluminium Ion Generated with the Pitting Corrosion of Aluminium)

  • 정경훈;정오진
    • 한국환경과학회지
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    • 제8권6호
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    • pp.705-710
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    • 1999
  • The fundamental experiments on the phosphorus removal from water were carried out by the batch and continuous reactors which used aluminium and copper plate. In this systems, the phosphorus was removed by aluminium ion generated with the electrochemical interaction (pitting corrosion) of aluminium and copper. In the batch experiments, the efficiencies of phosphorus removal increased when the surfaces of aluminium and copper plate were brushed. The phosphorus removal by aluminium ion was affected the copper plate and NaCI in this system. The optimal pH values were 5 and 6 for the phosphorus removal. The efficiency of phosphorus removal increased with increasing NaCI concentration, surface area of aluminium and copper plate. The $CUSO_4{\cdot}5H_2O$ instead of copper plate could be used as Cu source. The effluent $PO_4-P$ concentration as low as 2 $mg/{\ell}$ could have been obtained during the continuous experiment at HRT of 48 hrs.

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Removal of Total Suspended Solids by a Foam Fractionator in a Simulated Seawater Aquaculture System

  • Peng, Lei;Jo, Jae-Yoon
    • 한국양식학회지
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    • 제16권4호
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    • pp.216-222
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    • 2003
  • In a simulated seawater aquaculture system, effects of different operating factors like the superficial air velocity (SAY), hydraulic residence time (HRT), protein concentration and foam overflow height on the removal of total suspended solids (TSS) by a foam fractionator, with 20 cm diameter and 120 cm height, were investigated. This experiment was performed on batch and consecutive modes for different combinations of the tested factors, using synthetic wastewater. In 5 consecutive trials, TSS concentration in culture tank water decreased faster, when the foam fractionator was operated at higher SAV and lower HRT. In batch trials, with increasing SAV, TSS removal rate increased, but decreased with increasing HRT. Higher protein concentration in the bulk solution resulted in higher TSS removal rate. TSS concentration in the collected foam condensates increased but the foam overflow rate decreased with increasing foam overflow height. Foam fractionation was effective for removing TSS in seawater aquaculture systems and its performance largely depended on the operating parameters, especially superficial air velocity.

An accelerated sequential sampling for estimating the reliability of N-parallel systems

  • Rekab, Kamel;Cheng, Yuan
    • International Journal of Reliability and Applications
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    • 제14권2호
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    • pp.71-78
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    • 2013
  • The problem of designing an experiment to estimate the reliability of a system that has N subsystems connected in series where each subsystem n has n $T_n$ components connected in parallel is investigated both theoretically and by simulation. An accelerated sampling sheme is introduced. It is shown that the accelerated sampling scheme is asymptotically optimal as the total number of units goes to infinity. Numerical comparisons for a system that has two subsystems connected in series where each subsystem has two components connected in parallel are also given. They indicate that the accelerated sampling scheme performs better than the batch sequential sampling scheme and is nearly optimal.

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Advances for the time-dependent Monte Carlo neutron transport analysis in McCARD

  • Sang Hoon Jang;Hyung Jin Shim
    • Nuclear Engineering and Technology
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    • 제55권7호
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    • pp.2712-2722
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    • 2023
  • For an accurate and efficient time-dependent Monte Carlo (TDMC) neutron transport analysis, several advanced methods are newly developed and implemented in the Seoul National University Monte Carlo code, McCARD. For an efficient control of the neutron population, a dynamic weight window method is devised to adjust the weight bounds of the implicit capture in the time bin-by-bin TDMC simulations. A moving geometry module is developed to model a continuous insertion or withdrawal of a control rod. Especially, the history-based batch method for the TDMC calculations is developed to predict the unbiased variance of a bin-wise mean estimate. The developed methods are verified for three-dimensional problems in the C5G7-TD benchmark, showing good agreements with results from a deterministic neutron transport analysis code, nTRACER, within the statistical uncertainty bounds. In addition, the TDMC analysis capability implemented in McCARD is demonstrated to search the optimum detector positions for the pulsed-neutron-source experiments in the Kyoto University Critical Assembly and AGN201K.

GAL promoter에 적합한 효모변이주 Y334를 이용한 재조합 단백질 생산 최적화 방법 개발 (The Optimization of Recombinant Protein Production using S. cerevisiae Mutant Y334 Suitable for GAL Promoter)

  • 강환구;전희진;이문원
    • KSBB Journal
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    • 제15권2호
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    • pp.181-187
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    • 2000
  • 본 연구에서 갈락토즈를 거의 사용하지 않고 glucose repression 정도가 줄어든 변이주를 이용하여 fed-batch를 통한 발현최적화를 수행하였다. 두 균주에서의 GAL promoter에 의한 외래단백질 생산시 glucose repression 정도에 대해 조사하였는데 대조구 Y2805는 글루코즈가 다 소비된 후 2∼3시간 지난 후 발현이 시작되나 변이주 Y334는 약 0.5 g/L 글루코즈 농도에서 25%정도의 발현이 이루어짐에 따라 변이주 Y334는 GAL promoter에 미치는 glucose repression 정도가 매우 약한 장점을 확인하였다. 배양 중 재조합 미생물인 두균주 변이주 Y334와 대조구 효모 Y2805의 plasmid stability에 대해 안정한 균주임을 알 수 있었으며 대조구 Y2805의 경우도 plasmid stability는 90%로 유지됨을 알 수 있었다. GAL promoter에 의한 외래 단백질 생산시 글루코즈와 갈락토즈, 에탄올의 소비속도를 조사하였는데, 글루코즈와 에탄올의 소비속도는 거의 비슷하였으나 갈락토즈 소비속도는 Y2805는 0.1232 g/L/hr/O.D.이고 변이주 Y334는 0.0131 g/L/hr/O.D. 이다. 또한 mutant Y334와 대조구 효모 Y2805의 Fed-batch 시의 올바른 feeding 방법을 구하기위한 실험을 수행하여 각 균주의 Fed-batch 실험에서의 최적 발현 방법을 획득하였다.

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Significant Gene Selection Using Integrated Microarray Data Set with Batch Effect

  • Kim Ki-Yeol;Chung Hyun-Cheol;Jeung Hei-Cheul;Shin Ji-Hye;Kim Tae-Soo;Rha Sun-Young
    • Genomics & Informatics
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    • 제4권3호
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    • pp.110-117
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    • 2006
  • In microarray technology, many diverse experimental features can cause biases including RNA sources, microarray production or different platforms, diverse sample processing and various experiment protocols. These systematic effects cause a substantial obstacle in the analysis of microarray data. When such data sets derived from different experimental processes were used, the analysis result was almost inconsistent and it is not reliable. Therefore, one of the most pressing challenges in the microarray field is how to combine data that comes from two different groups. As the novel trial to integrate two data sets with batch effect, we simply applied standardization to microarray data before the significant gene selection. In the gene selection step, we used new defined measure that considers the distance between a gene and an ideal gene as well as the between-slide and within-slide variations. Also we discussed the association of biological functions and different expression patterns in selected discriminative gene set. As a result, we could confirm that batch effect was minimized by standardization and the selected genes from the standardized data included various expression pattems and the significant biological functions.