• Mycobiology
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• 제40권2호
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• pp.107-110
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• 2012

Genes encoding the cellobiohydrolase enzyme (CBHI), designated as cbhI, were isolated from the basidiomycetes Auricularia fuscosuccinea, Pleurotus giganteus, P. eryngii, P. ostreatus, and P. sajor-caju. Initially, the fungal genomic DNA was extracted using a modified cetyltrimethyl ammonium bromide (CTAB) protocol and used as a DNA template. The cbhI genes were then amplified and cloned using the pGEM-T Easy Vector Systems. The sizes of these PCR amplicons were between 700~800 bp. The DNA sequences obtained were similar showing high identity to the cbhI gene family. These cbhI genes were partial consisting of three coding regions and two introns. The deduced amino acid sequences exhibited significant similarity to those of fungal CBHI enzymes belonging to glycosyl hydrolase family 7.

• 한국버섯학회지
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• 제8권3호
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• pp.102-108
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• 2010

본 연구는 곡류별 버섯 균사체의 성장률과 효소활성을 측정하였다. 현맥 배양물이 다른 곡류와 비교했을때 균사체 성장 속도가 빨랐다. 효소 활성은 기간별로 측정하였으며 그 결과, ${\alpha}$-amylase 효소 활성은 느타리와 상황버섯균이 우수하였으며, ${\beta}$-amylase활성은 느타리와 장수버섯균이 우수하였다. Protease활성은 상황버섯균을 제외하고는 곡물에 관계없이 비교적 고른 생산을 보였다. 담자균 배양에 의해서 명도는 감소하고 적색도와 황색도가 증가하였다.

• 환경생물
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• 제23권4호
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• pp.335-342
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• 2005

We cloned seven genes encoding chitin synthases (CHSs) by PCR amplification from genomic DNAs of four strains of the genus Sporobolomyces and of Bensingtonia subrosea using degenerated primers based on conserved regions of the CHS genes. Though amino acid sequences of these genes were shown similar as 176 to 189 amino acids except SgCHS2, DNA sequences were different in size, which was due to various introns present in seven fragments. Alignment and phylogenetic analysis of their deduced amino acid sequences together with the reported CHS genes of basidiomycetes separated the sequences into classes I, II and III. This analysis also permitted the classification of isolated CHSs; SgCHS1 belongs to class I, BsCHS1, SaCHS1, SgCHS2, SpgCHS1, and SsCHS1 belong to class II, and BsCHS2 belongs to class III. The deduced amino acid sequences involving in class II that were discovered from five strains were also compared with those of other basidiomycetes by CLUSTAL X program. The bootstrap analysis and phylogenetic tree by neighbor-joining method revealed the taxonomic and evolutionary position for four strains of the genus Sporobolomyces and for Bensingtonia subrosea which agreed with the previous classification. The results clearly showed that CHS fragments could be used as a valuable key for the molecular taxonomic and phylogenetic studies of basidiomycetes.

• 한국균학회지
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• 제32권2호
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• pp.148-151
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• 2004

본 연구는 Phellinus linteus와 관련 담자균류 속간의 IGS I 영역의 분석에 기초한 분류체계를 정립하기 위해서 실시하였다. 실험에 사용된 Phellinus linteus 균주의 IGS I 영역은 730 bp였고 다른 담자균류의 IGS I 영역과 비교시 5' 말단부위의 $1{\sim}280\;base$까지는 각 균주들간의 보존성이 높게 나타났으며 3' 말단부위로 갈수록 보존성이 감소하다가 다시 증가하는 경향을 보였다. 담자균류의 IGS 영역에 대한 연구가 앞으로 진행된다면 ITS 영역의 비교와 더불어 새로운 계통분류지표로 이용될 수 있을 것이다.

• Archives of Pharmacal Research
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• 제5권1호
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• pp.17-19
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• 1982

To develop potent anitumor polysac-charides from higher hungi of Korea the alcohol precipitated polysaccharides, which were obtained from hot water extracts of shake-cultured mycelia of eight species of basidiomycetes, were subjected to antitumor screening tests, using sarcoma 180 as a tumor cell. When administered i. p, at the dose level of 20mg/kg/day for ten days into the mice which were implanted with $1{times}10^{8}$ cells of sarcoma 180 twenty-four hour before the start of polysaccharide injection, the polysaccharides of Plutcus cervinus ISA-Pc-1004, Pleurotus pulmonarius, Hydnum repandum ISA-Hr-1006, and Laccaria laccata ISA-LI-1008 respectively showed inhibition ratios of 62.0%, 51.7%, 64.8%, and 57.8%. They were selected for further study, while those of Pancillus serotina ISA-Ps-1012, Laccaria amethystina ISA-La 1001, Pholiota adiposa ISA-Pa-1010, and Lepista spISA-Ls-1007 respectively showed inhibition ratios of -9.3%, 25.3%, 27.3% and 33.0%.

• 한국균학회지
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• 제25권4호통권83호
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• pp.253-256
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• 1997

한국산 야생버섯의 독성을 규명하기 위한 연구의 일환으로 46종의 야생버섯의 용혈독성을 검색하여 다음과 같은 결과를 얻었다. 1) 46종 중 Amanita esculenta, A. griseofarinosa, A. longistriata, A. melleiceps, A. phalloides, A. rubescence, A. spissacea, A. vaginata, Hebeloma crustuliniforme, Lepiota cygnea, Macrolepiota procera, 및 Oudemansiella platyphylla 등 12종의 야생 담자균 냉침액이 마우스 적혈구에 대하여 강력한 용혈활성을 나타내었다. 2) A. griseofarinosa 및 H. crustuliniforme은 $100^{\circ}C$ 수용상에서 5분간 가열하여도 그 용혈 활성이 소실되지 않아 열에 매우 안정한 용혈성분을 함유하는 것으로 추정된다.

• 한국균학회지
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• 제14권3호
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• pp.231-235
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• 1986

몇가지 목재부식성(木材腐蝕性) 담자균류(擔子菌類)의 포자(胞子)를 시간별로 수중(水中)에 담그는 방법(方法)을 사용하여 포자(布子)를 발아(發芽)시켜본 결과(結果) 한가지 균주에서 포자 발아가 억제 되었다. 침수시간(浸水時間)을 증가시킬수록 포자 발아가 증가(增加)된 균주는 5종(種)이었으며 포자발아가 전혀 일어나지 않는 균주는 1종(種)이었다. 이것은 포자(布子)의 원래 성질(性質) 및 항상성(恒常性)과 관계가 깊었다. 모든 시험균주는 침수(侵水) 직후(直後)가 최대(最大) 수분(水分) 흡수율(吸水率)을 나타내었으며 침수시간이 경과할수록 수분 흡수율은 저하하였다. 포자의 탈수속도(說水速度)를 비교한 결과, 2종(種)의 포자는 신속히 수분을 상실하였으나, 3(種)의 포자는 완만히 수분을 상실하였다. 즉 부피가 크고 단단한 포자보다 작으면서 섬세한 포자의 탈수속도(脫水速度)가 컸다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제5권6호
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• pp.422-430
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• 2000

Mechanism of lignin biodegradation caused by basidiomycetes and the history of lignin biodegradation studies were briefly reviewed. The important roles of fungal extracellular ligninolytic enzymes such as lignin and manganese peroxidases (LiP and MnP) were also summarized. These enzymes were unique in their catalytic mechanisms and substrate specificities. Either LiP or MnP system is capable of oxidizing a variety of aromatic substrates via a one-electron oxidation. Extracellular fungal system for aromatic degradation is non-specific, which recently attracts many people working a bioremediation field. On the other hand, an intracellular degradation system for aromatic compounds is rather specific in the fungal cell. Structurally similar compounds were prepared and metabolized, indicating that an intracellular degradation strategy consisted of the cellular systems for substrate recognition and metabolic response. It was assumed that lignin-degrading fungi might be needed to develop multiple metabolic pathways for a variety of aromatic compounds caused by the action of non-specific ligninolytic enzymes on lignin. Our recent results on chemical stress responsible factors analyzed using mRNA differential display techniques were also mentioned.

• 생약학회지
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• 제17권1호
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• pp.39-48
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• 1986

To find antitumor components in the shake-cultured mycelia of Volvariella bombycina, the mycelia were extracted with hot water. After the extract was dialyzed and freeze-dried, a protein-polysaccharide fraction was obtained and examined for antitumor activity against the solid form of sarcoma 180 in ICR mice. It showed 60.3% inhibition ratio at a dose of 20mg/kg/day for 10 days. It was found to consist of a polysaccharide moiety and a protein moiety. After gel filtration on Sepharose 4B, Fraction B was obtained and showed the highest inhibition ratio of 71.1%. When the antitumor component was examined for immunopotentiating activity, it was found to increase the macrophage accumulation in the peritoneal cavity as well as the antibody production of the spleen cells of the mice.

• 한국균학회지
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• 제3권2호
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• pp.31-33
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• 1975

1973년(年)부터 1975(年)까지 수원(水原), 광릉(光陵), 대관령(大關嶺), 지리산(智異山) 및 한봉산등(漢拏山等) 5개(個) 지역(地域)에서 132종(種)의 고등균류(高等菌類)를 채집(採集)하여 동정(同定)하였고, 그 중에서 12과(科), 37종(種)이 한국미기록종(韓國未記種)이였음이 밝혀졌으며 10종(種)은 식용(食用)버섯이었고 27종(種)은 식용(食用)이 불가능(不可能)한 종(種)들이었다. 이들 버섯의 우리나라 이름이 저저(著者)들에 의해서 신청(新稱)되었다.