Mycobiology

The Korean Society of Mycology (한국균학회)
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Cloning and Sequence Analysis of the Cellobiohydrolase I Genes from Some Basidiomycetes

  • Chukeatirote, Ekachai (Institute of Excellence in Fungal Research, Mae Fah Luang University) ;
  • Maharachchikumbura, Sajeewa S.N. (Institute of Excellence in Fungal Research, Mae Fah Luang University) ;
  • Wongkham, Shannaphimon (Scientific & Technological Instruments Centre, Mae Fah Luang University) ;
  • Sysouphanthong, Phongeun (Institute of Excellence in Fungal Research, Mae Fah Luang University) ;
  • Phookamsak, Rungtiwa (Institute of Excellence in Fungal Research, Mae Fah Luang University) ;
  • Hyde, Kevin D. (Institute of Excellence in Fungal Research, Mae Fah Luang University)
  • Received : 2012.04.04
  • Accepted : 2012.05.05
  • Published : 2012.06.30
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Abstract

Genes encoding the cellobiohydrolase enzyme (CBHI), designated as cbhI, were isolated from the basidiomycetes Auricularia fuscosuccinea, Pleurotus giganteus, P. eryngii, P. ostreatus, and P. sajor-caju. Initially, the fungal genomic DNA was extracted using a modified cetyltrimethyl ammonium bromide (CTAB) protocol and used as a DNA template. The cbhI genes were then amplified and cloned using the pGEM-T Easy Vector Systems. The sizes of these PCR amplicons were between 700~800 bp. The DNA sequences obtained were similar showing high identity to the cbhI gene family. These cbhI genes were partial consisting of three coding regions and two introns. The deduced amino acid sequences exhibited significant similarity to those of fungal CBHI enzymes belonging to glycosyl hydrolase family 7.

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References

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