• 한국균학회지
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• 제13권1호
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• pp.53-55
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• 1985

한국(韓國)에서 인공재배( 人工栽培)한 영지(靈芝)의 회분(灰分)은 갓과 녹각지가 각각 1.48%와 1.40% 였으며, 무기(無機) 성분(成分)은 갓부분에서는 Ca이 가장 많이 함유(含有)되어 있었으며, 그 다음은 Mg, Na, Mn, Fe, Zn, Cu, Ge의 순서로 함유(含有)되어 있었다. 녹각지에서는 Mg이 가장 많이 함유(含有)되어 있었으며, 그 다음은 Ca, Na, Zn, Mn, Fe, Cu, Ge의 순시로 함유(含有)되어 있었다.

• Mycobiology
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• 제47권4호
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• pp.521-526
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• 2019

During the 2014 survey of the mushroom flora of Gwangneung forest in South Korea, we collected two specimens of boletoid mushroom growing on a felled tree of Pinus koraiensis. These specimens were characterized by a light brown to reddish-brown pileus with appressed tomentum, pore surface bluing instantly when bruised, golden-yellow mycelium at the base of stipe, and lignicolous habitat. Both specimens were identified as Buchwaldoboletus lignicola, a rare basidiomycete, based on morphological characteristics and sequences of internal transcribed spacer (ITS; fungal barcode). Here, we describe these specimens and provide the first report of this genus in South Korea.

• 생약학회지
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• 제30권3호
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• pp.244-249
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• 1999

In order to find less toxic antiviral agents from Basidiomycetes, EA, the water soluble substance, was prepared from the carpophores of Elfvingia applanata(Pers.) Karst. Antiviral activity of EA against vesicular stomatitis virus [Indiana serotype, VSV(IND)] was examined in Vero cells using plaque reduction assay in vitro. And the combined antiviral effects of EA with interferon (IFN) alpha or gamma were examined on the multiplication of VSV(IND). EA caused a concentration-dependent reduction in the plaque formation of VSV(IND) with 50% effective concentration $(EC_{50})$ of $104.02\;{\mu}g/ml$. The results of combination assay were evaluated by the combination index (CI) that was analysed by the multiple drug effect analysis. All cases of the combination of EA with IFN alpha or IFN gamma showed potent synergism with CI values of $0.38{\sim}0.52$ for $50{\sim}90%$ effective levels.

• 생약학회지
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• 제27권3호
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• pp.224-230
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• 1996

To find pharmacologically active hybrids among the inter-order protoplast fusants of Lentinula edodes and Ginoderma lucidam the antitumor test was performed and the fusant P22 was selected among them. The hot water and alkaline extracts from the cultured mycelia of P22 were purified and separated into four fractions by DEAE-cellulose anion exchange chromatography. When a dose of 20 mg/kg/day of each fractions was injected into ICR mice by i.p., the tumor inhibition ratio of Fr. IV against solid sarcoma 180 was the higher than any other fraction. Fr. IV was a protein-bound polysaccharide which was composed of 69. 12% polysacchafide and 9.76% protein and the molecular weight of Fr. IV was $6.7{\times}10^4$ dalton.

• 한국균학회지
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• 제13권3호
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• pp.185-189
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• 1985

식용버섯의 유전 연구와 균주 개발을 위하여 표고버섯의 담자포자에 자외선을 조사하여 영양요구성 균주를 유발하였는바, 자외선을 담자포자에 40초 조사했을때 약 5 %의 포자 생존율을 보였으며 120초 조사에서 완전히 발아력을 상실하였다. 30초에서 100초까지 10초 간격으로 자외선을 조사하여 7088 colonies를 검정한 결과 71개의 auxotrophs를 선발하여 1.0%의 유발율을 얻었으며, 60초 조사에서 1.35 %의 가장 높은 유발 빈도를 나타내었는데 이때의 포자 생존율은 1.3% 이었다. 71개의 auxotrrophs 중에서 29개 균주의 영양요구성을 검정한 결과 nucleic acid-requiring strain이 3, nucleic acid/vitamin-requiring strain이 13, nucleic acid/amino acid-requiring strain이 1, vitamin-requiring strain이 7, 그리고 amino acid-requiring strain이 5이었다.

• 한국균학회지
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• 제13권2호
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• pp.75-78
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• 1985

진균류의 균주개발과 유전연구에 필수적인 영양요구성 균주 선발을 위하여 느타리비섯과 사철느타리버섯의 월형질체에 자외선을 조사하여 얻은 결과를 요약하면 다음과 같다. 1. 원형 질체에 자외선을 조사하여 그 생존을이$0.83{\sim}15%$일 때 가장 많은 영양 요구성 균주를 선발하였다. 2. 느타리에서 Arg, Gly Ser 그리고 Ala Orn Tyr 3균주와 사철느타리버섯에서 Ribo-1, Ribo-2, Phen, 그리고, Ade Hyp 4군주를 선발하였으며 이들 중에서 Phen 균주는 back mutation 현상이 나타났다.

• Mycobiology
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• 제34권4호
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• pp.200-205
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• 2006

Phellinus genus belonged to Hymenochaetaceae of Basidiomycetes and has been well known as one of the most popular medicinal mushrooms due to high antitumor activity. This study was carried out to obtain the basic information for mycelial culture conditions of Phellinus linteus, P. baumii, and P. gilvus. According to colony diameter and mycelial density, the media for suitable mycelial growth of them were shown in MEA, glucose peptone, and MCM. The optimum temperature for mycelial growth was $30^{\circ}C$. Carbon and nitrogen sources were man nose and malt extract, respectively. The optimum C/N ratio was 10:1 to 5:1 with 2% glucose concentration, vitamin was thiamine-HCl, organic acid was succinic acid, and mineral salt was $MgSO_{4}{\cdot}7H_{2}O$.

• 약학회지
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• 제45권1호
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• pp.64-70
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• 2001

Protein-polysaccharide fractions, PJ-3 and PJ-4, were prepared from mycelial culture filtrate of an insect-born fungus, Paecilomyces japonica DGUM 32001, and subjected to a flow cytometrical analysis for their vivo antitumor and immunomodulating activity in ICR mice. When i.p. injected once daily for semen days at 100 mg/kg, PJ-4 exerted a strong antitumor activity showing the growth inhibition ratio of 85.1% against i.p. implanted sarcoma 180 cells, while PJ-3 showed only a weak activity. Moreover, PJ-4 signiscantly increased the expression level of CD25 (IL-2R $\alpha$-chain) as well as forward scatter (FSC) values of splenic CD8$^{8}$ T cells. It is also noteworthy that PJ-4 strongly induced the peritoneal exudate cells in the same experiment. In an in vitro study, PJ-4 slightly inhibited the growth of sarcoma 180 cells at the concentration of 50$\mu$g/ml or higher. These results strongly suggest that PJ-4 might exert its antitumor activity through immunostimulation as well as direct inhibitory activity on the tumor cells.

• Journal of Microbiology
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• 제43권6호
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• pp.503-509
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• 2005

The production of manganese peroxidase (MnP) by Irpex lacteus, purified to electrophoretic homogeneity by acetone precipitation, HiPrep Q and HiPrep Sephacryl S-200 chromatography, was shown to correlate with the decolorization of textile industry wastewater. The MnP was purified 11.0-fold, with an overall yield of $24.3\%$. The molecular mass of the native enzyme, as determined by gel filtration chromatography, was about 53 kDa. The enzyme was shown to have a molecular mass of 53.2 and 38.3 kDa on SDS-PAGE and MALDI-TOF mass spectrometry, respectively, and an isoelectric point of about 3.7. The enzyme was optimally active at pH 6.0 and between 30 and $40^{\circ}C$. The enzyme efficiently catalyzed the decolorization of various artificial dyes and oxidized Mn (II) to Mn (III) in the presence of $H_2O_2$. The absorption spectrum of the enzyme exhibited maxima at 407, 500, and 640 nm. The amino acid sequence of the three tryptic peptides was analyzed by ESI Q- TOF MS/MS spectrometry, and showed low similarity to those of the extracellular peroxidases of other white-rot basidiomycetes.

• 한국미생물·생명공학회지
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• 제14권4호
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• pp.279-283
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• 1986

홍삼제품 제조 과정중의 부산물인 홍삼박의 활용 가능성을 조사하기 위하여 여러 가지 담자균의 배양에 홍삼박 추출물을 첨가하여 배양한 후 균체 증식량, 단백질 및 amino acid함량 등을 조사하였으며 그 결과를 보고하는 바이다. 수집 된 담자균 중 G. lucidum과 P. ostreatus가 액체배양에서 균체증식이 양호한 균주로 선발되었으며, 홍삼박 추출물을 0.2％ 첨가하였을 때 균체증식량이 가장 많았으며 단백질 함량은 0.5％ 첨가하였을 때 l-3％가 증가하였다. Amino acid함량은 인삼박 추출물을 0.2％ 첨가하였을 때는 거의 변화가 없었으나 0.5％ 첨가하였을 때에는 G. lucidum, P. ostreatus 모두 상당히 증가하였으며 그 증가폭 중 cysteine이 가장 컸다.