• 한국통신학회논문지
• /
• 제19권5호
• /
• pp.971-980
• /
• 1994

현존하는 셀룰라 이동통신의 가입자 수용 한계로 부호분할다원접속(CDMA) 방식을 사용하는 디지틀셀룰라 통신 방식이 거론되고 있다. EIA/TIA 잠정 표준안인 CDMA 시스팀에서는 동일한 확살부호를 전체 시스팀에 사용하지만 각 기지국에 확산부호의 위상옵셉(phase-offset)을 다르게 배분함으로써 이동국은 기지국으로 송신된 신호를 구변하도록 하고 있다. 그러나 확산부호의 위상옵셉이 기준이 되는 영옵셉(zero-offset) 부호를 임의로 설정하고 있다. 본 논문은 확산부호의 기준 부호를 정하는 방법을 제시하고, 기준 부호로부터 위상을 이전(shift) 시킨 부호 사이의 위상옵셉을 쉽게 계산하는 방법을 제시한다.

• Archives of Pharmacal Research
• /
• 제23권2호
• /
• pp.187-195
• /
• 2000

Nucleotide sequence extending 2,3-dihydroxybiphenyl 1,2-dioxygenase gene (pcbC) and 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase gene (pcbD) of Pseudomonas sp. DJ-12 was previously analyzed and the two genes were present in the order of pcbD-pcbC preceded by a promoter from Pseudomonas sp. DJ-12. In this study, a 3.8-kb nucleotide sequence located downstream of the pcbC gene was analyzed to have three open reading frames (ORFs) that are designated as orf1, pcbE and orf2 genes. All of the ORFs were preceded by each ribosome-binding sequence of 5-GGAXA-3 (X=G or A). However, no promoter-like sequence and transcription terminator sequence were found in the analyzed region, downstream of pcbC gene. Therefore, the gene cluster appeared to be present in the order of pcbD-pcbC-orf1-pcbE-orf2 as an operon, which is unique organization characterized so far in biphenyl- and PCB-degrading bacteria. The orf1 gene was composed of 1,224 base pairs which can encode a polypeptide of molecular weight 44,950 containing 405 amino acid residues. A deduced amino acid sequence of the orf1 gene product exhibited 21-33％ identity with those of indole dioxygenase and phenol hydroxylase components. The pcbE gene was composed of 783 base pairs encoding 2-hydroxypenta-2,4-dienoate hydratase involved in the 4-chlorobiphenyl catabolism. The orf2 gene was composed of 1,017 base pairs encoding a polypeptide of molecular weight 37,378 containing 338 amino acid residues. A deduced amino acid sequence of the orf2 gene product exhibited 31％ identity with that of a nitrilotriacetate monooxygenase component.

• 대한전자공학회:학술대회논문집
• /
• 대한전자공학회 2003년도 신호처리소사이어티 추계학술대회 논문집
• /
• pp.199-202
• /
• 2003

In this paper, we propose a new 3D coding method for heterogeneous systems over enhanced Access Grid (e-AG) with 3D display using spatio-temporal scalability. The proposed encoder produces four bit-streams: one base layer and enhancement layer l, 2 and 3. The base layer represents a video sequence for left eye with lower spatial resolution. An enhancement layer l provides additional bit-stream needed for reproduction of frames produced in base layer with full resolution. Similarly, the enhancement layer 2 represents a video sequence for right eye with lower spatial resolution and an enhancement layer 3 provides additional bit-stream needed for reproduction of its reference pictures with full resolution. In this system, temporal resolution reduction is obtained by dropping B-frames in the receiver according to network condition. The receiver system can select the spatial and temporal resolution of video sequence with its display condition by properly combining bit-streams.

• Journal of Plant Biology
• /
• 제38권3호
• /
• pp.259-266
• /
• 1995

To investigate how the dyad symmetry region (DSR) and the distance between vir box and -35 sequence of the virE promoter plays a role in virE gene expression, two mutants were constructed by base substitution and insertional mutagenesis. The base substitutional mutation, a AAlongrightarrowCG substitution at positions -39 and -40 on the DSR, showed the level of $\beta$-galactosidase activity approximately 91% of the wild type virE promoter activity. Therefore, the native structure of the DSR seems to be not essential for virE expression. The insertional mutation, constructed by inserting 8 bp ClaI linker between -49 and -50, displayed the $\beta$-galactosidase activity at 12% of the native virE promoter activity. However, this striking reduction appears to be not caused by destruction of the native DSR structure, but by shifting the vir box far from putative -35 sequence.

• 생명과학회지
• /
• 제14권6호
• /
• pp.963-966
• /
• 2004

능이버섯의 16S ribosomal DNA일부분, IT1, 5.85 ribosomal DNA, ITS2의 전부분, 28S ribosomal DNA 일부분이 포함된 ITS영역의 염기서열을 분석하였다. 이 부분은 716개의 염기쌍으로 구성되었다. 이를 Sarcadon속에 속하는 종들과 비교분석한 결과 같은 능이버섯의 ITS에 관한 다른 분석결과 염기치환 및 결실을 근거로 하였을 경우 $1.8\%$의 차이를 나타내었다. 또한 S. imbricatus와는 $1.8\%$, S. sequamous와는 $10\%$차이를 나타내었다. 이는 능이버섯이 숙주, 서식지 환경 등의 특수성 때문에 자연상태에서 유전자교류가 일어나지 않기 때문인 것으로 생각된다.

• 대한바이러스학회지
• /
• 제30권1호
• /
• pp.1-10
• /
• 2000

The nonstructural protein NSP4, encoded by gene 10 of rotavirus, has been shown to playa role in viral assembly and known to be an enterotoxin, causing diarrhea in mouse pups. NSP4 gene was cloned from CBNU-2 (virulent bovine rotavirus/diarrheic fecal sample) and CBNU-1 (cell-culture adapted bovine rotavirus/isolated from CBNU-2 and 75 times passaged on MA104 cells), respectively, by reverse transcriptase-polymerase chain reaction (RT-PCR) and sequenced and compared. The sequence data indicated that the NSP4 genes of bovine rotavirus (BRV) were 751 bases in length and encoded one open reading frame of 175 amino acids beginning at base 42 and terminating at base 569. Differences in nucleotide sequence between CBNU-2 and CBNU-1 were observed at 6 positions (base 274, 296, 391, 394, 396 and 579). NSP4 gene of BRV exhibited a high degree of nucleotide (90% and 94%) and amino acid sequence (91% and 97%) homology with those of SA11 and UK but a low degree of nucleotide (77% and 79%) and amino acids sequence (81% and 85%) homology with those of Wa and OSU.

• 미생물학회지
• /
• 제32권1호
• /
• pp.20-27
• /
• 1994

항생물질에 대한 다중 저항성을 갖는 Staphylococcus aureus 균주의 chromosomal DNA로부터 유발성 발현을 하는 ${\beta}$-lactamase(bla) 유전자를 확인, 분리하였다. Cloning에 이어 결정된 염기서열을, S. aureus 에서는 지금까지는 plasmid상에서만 분리, 보고되어 있는 bla 유전자들의 염기서열과 비교하였다. 본 bla 유전자의 구조유전자 부분인 843base의 염기서열은 기 발표된 pPC1, pl258, pS1, pI1071, pUB101, pl3796 및 pI3804 유래의 bla 유전자들 중 pPC1, pI258 및 pS1상에 존재하는 bla 구조유전자의 염기서열과 완전히 일치하였고 나머지 것들과도 매우 높은 상동성(99%)을 유지하고 있었다. Bla구조 유전자의 상류 370base 및 하류 220base까지 결정된 염기서열을 비교한 결과에서는 다른 모든 bla구조유전자의 상류 150base에 위치하는 HindIII 인식부위가 약 230base 이상 더 윗쪽으로 옮겨가 있었고 이 HindIII 인식부위를 포함하는 염기서열에서 ORF의 C말단이 발견되었다. 하류 서열에서는 pI1071 유래 bla가 갖는 두개의 직접반복 염기서열 중 하나가 결손된 형태를 보이고 있다. 구조 유전자 상류에 존재하는, 80개 아미노산으로 구성된 ORF의 상동성 검색 결과 Tn4001 의 transposase 의 C 말단과 일치함이 발견되었다.

• Mycobiology
• /
• 제33권2호
• /
• pp.109-112
• /
• 2005

The internal transcribed spacer (ITS) regions including the 3'-end of 18S rRNA gene, 5.8S rRNA gene and the 5'-end of the 28S rRNA gene of Rhizopus spp. were amplified by PCR and analyzed by DNASIS program. Length polymorphism of these region ranged from 564 bp in R. oryzae to 789bp in R. stolonifer. The length and sequence of 5.8S was very conserved with $154{\sim}155\;bp$. The sequence of ITS2 was more variable than that of ITS1. The base substitution rates were ranged from 0 to 0.6069 per site, and higher rate was found in R. stolonifer. In general, transition was usually more frequent than transversion. On the basis of sequencing results, four groups were clustered with value of 61.9% similarity; R. oryzae, R. micros pores, R. homothallicus, and R. stolonifer groups.

• BMB Reports
• /
• 제32권4호
• /
• pp.399-404
• /
• 1999

2,3-Dihydroxybiphenyl 1,2-dioxygenase (2,3-DHBD), which catalyzes the ring meta-cleavage of 2,3-dihydroxybiphenyl, is encoded by the phnQ gene of biphenyl- and phenanthrene-degrading Pseudomonas sp. strain DJ77. We determined the nucleotide sequence of a DNA fragment of 1497 base pairs which included the phnQ gene. The fragment lncluded an open reading frame of 903 base pairs to accommodate the enzyme. The predicted amino acid sequence of the enzyme subunit consisted of 300 residues. In front of the gene, a sequence resembling an E. coli promoter was identified, which led to constitutive expression of the cloned gene in E. coli. The deduced amino acid sequence of the PhnQ enzyme exhibited 85.6% identity with that of the corresponding enzyme in Sphingomonas yanoikuyae Q1 (formerly S. paucimobilis Q1) and 22.1% identity with that of catechol 1,2,3-dioxygenase from the same DJ77 strain. PhnQ showed broader substrate preference than previously-cloned PhnE, catechol 2,3-dioxygenase. Ten amino acid residues, considered to be important for the role of extradiol dioxygenases, were conserved.

• 한국수산과학회지
• /
• 제33권6호
• /
• pp.489-495
• /
• 2000

The 185 ribosomal RNA gene (185 rDNA) of the marine alga Porphyra sp. 723 (Bangiales, Rhodophyta) was amplified using the polymerase chain reaction and its sequence was analysed. The Porphyra species was a summer strain collected on rocks in upper intertidal zone at Ikidae, Pusan on 23rd July 1999. The fronds were $1{\~}5 cm$ long, monostromatic, and orbicular or ovate shaped, They had spinulate processes at margin of the frond, Comparison of this 185 rDNA sequence with the other Forphyra species indicates that Porphyra sp. 723 has the same 185 rDNA sequence derived from Porphyra suborbiculata (NCBI access number; AB 013180) except one base pair substitution in 2327 base pairs.