• 제목/요약/키워드: Base pair

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Molecular characterization of Plasmodium juxtanucleare in Thai native fowls based on partial cytochrome C oxidase subunit I gene

  • Pohuang, Tawatchai;Junnu, Sucheeva
    • 대한수의학회지
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    • 제59권2호
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    • pp.69-74
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    • 2019
  • Avian malaria is one of the most important general blood parasites of poultry in Southeast Asia. Plasmodium (P.) juxtanucleare causes avian malaria in wild and domestic fowl. This study aimed to identify and characterize the Plasmodium species infecting in Thai native fowl. Blood samples were collected for microscopic examination, followed by detection of the Plasmodium cox I gene by using PCR. Five of the 10 sampled fowl had the desired 588 base pair amplicons. Sequence analysis of the five amplicons indicated that the nucleotide and amino acid sequences were homologous to each other and were closely related (100% identity) to a P. juxtanucleare strain isolated in Japan (AB250415). Furthermore, the phylogenetic tree of the cox I gene showed that the P. juxtanucleare in this study were grouped together and clustered with the Japan strain. The presence of P. juxtanucleare described in this study is the first report of P. juxtanucleare in the Thai native fowl of Thailand.

A new record of Haplodrassus nojimai Kamura, 2007 (Araneae, Gnaphosidae) from Korea

  • Kim, Seung Tae;Yoo, Jung Sun
    • 환경생물
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    • 제39권1호
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    • pp.16-18
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    • 2021
  • Haplodrassus nojimai Kamura, 2007 of Gnaphosidae, which is only known in Japan was newly described in Korea with measurements and morphological illustrations. A female was collected from the levee of rice fields using a pitfall trap. The female epigynum has thick and remarkable transverse wrinkles and is densely covered with long blackish-gray hairs. The anterior hood was slightly rounded and the lateral margins were relatively short. The median septum was V-shaped with a broad base on the median part, and a pair of spermathecae was visible. The male is still unknown.

Complete genome sequences of Lactococcus lactis JNU 534, a potential food and feed preservative

  • Sangdon, Ryu;Kiyeop, Kim;Dae-Yeon, Cho;Younghoon, Kim;Sejong, Oh
    • Journal of Animal Science and Technology
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    • 제64권3호
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    • pp.599-602
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    • 2022
  • A new bacteriocin-producing lactic acid bacteria isolated from kimchi was identified as Lactococcus lactis JNU 534, presenting preservative properties for foods of animal origin. In this study, we present the complete genome sequence of the bacterial strain JNU 534. The final complete genome assembly consists of one circular chromosome (2,443,687 bp [base pair]) with an overall GC (guanine-cytosine) content of 35.2%, one circular plasmid sequence (46,387bp) with a GC content of 34.5%, and one circular contig sequence (7,666 bp) with a GC content of 36.2%.

The complete chloroplast genome sequence of Rhododendron caucasicum (Ericaceae)

  • Myounghai KWAK;Rainer W. BUSSMANN
    • 식물분류학회지
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    • 제53권3호
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    • pp.230-236
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    • 2023
  • Rhododendron caucasicum Pall. is a shrub distributed in the mountainous areas of the Caucasus from northeastern Türkiye towards the Caspian Sea. This study reports the first complete chloroplast genome sequence of R. caucasicum. The plastome is 199,487 base pairs (bp) long and exhibits a typical quadripartite structure comprising a large single-copy region of 107,645 bp, a small single-copy region of 2,598 bp, and a pair of identical inverted repeat regions of 44,622 bp each. It contains 143 genes, comprising 93 protein-coding genes, 42 tRNA genes, and eight rRNA genes. The large chloroplast genome size is likely due to the expansion of inverted repeats. A phylogenetic analysis of chloroplast genomes with other Rhododendron species supports previously recognized infrageneric relationship.

Complete genome sequence of functional probiotic candidate Lactobacillus amylovorus CACC736

  • Soyeon Park;Jung-Ae Kim;Hyun-Jun Jang;Dae-Hyuk Kim;Yangseon Kim
    • Journal of Animal Science and Technology
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    • 제65권2호
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    • pp.473-477
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    • 2023
  • Lactobacillus amylovorus CACC736 was originated from swine feces in Korea. The complete genome sequences of the strain contained one circular chromosome (2,057,809 base pair [bp]) with 38.2% guanine-cytosine (GC) content and two circular plasmids, namely, pCACC736-1 and pCACC736-2. The predicted protein-coding genes, which are encoding the clustered regularly interspaced short palindromic repeats (CRISPR)-associated proteins, biosynthesis of bacteriocin (helveticin J), and the related proteins of the bile, acid tolerance. Notably, the genes related to vitamin B-group biosynthesis (riboflavin and cobalamin) were also found in L. amylovorus CACC736. Collectively, the complete genome sequence of the L. amylovorus CACC736 will aid in the development of functional probiotics in the animal industry.

Complete genome and two plasmids sequences of Lactiplantibacillus plantarum L55 for probiotic potentials

  • Bogun Kim;Kiyeop Kim;Xiaoyue Xu;Hyunju Lee;Duleepa Pathiraja;Dong-June Park;In-Geol Choi;Sejong Oh
    • Journal of Animal Science and Technology
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    • 제65권6호
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    • pp.1341-1344
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    • 2023
  • In this study, we report the complete genome sequence of Lactiplantibacillus plantarum L55, a probiotic strain of lactic acid bacteria isolated from kimchi. The genome consists of one circular chromosome (2,077,416 base pair [bp]) with a guanine cytosine (GC) content of 44.5%, and two circular plasmid sequences (54,267 and 19,592 bp, respectively). We also conducted a comprehensive analysis of the genome, which identified the presence of functional genes, genomic islands, and antibiotic-resistance genes. The genome sequence data presented in this study provide insights into the genetic basis of L. plantarum L55, which could be beneficial for the future development of probiotic applications.

돼지 150-kDa Insulin-like Growth Factor Complex의 Acid-labile Subunit(ALS) 유전자의 Intron 및 ALS Complementary DNA의 3' 비해독 부위 Cloning과 생체조직에서의 ALS 유전자 발현 확인 (Cloning of An Intron of the Gene Coding for Porcine Acid-Labile Subunit(pALS) of the 150-kDa Insulin-like Growth Factor Complex and the 3' ntranslated Region of pALS Complementary DNA and Confirmation of pALS Gene Expression in Multiple Tissues)

  • 진은정;김인애;이철영
    • Journal of Animal Science and Technology
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    • 제46권4호
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    • pp.555-562
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    • 2004
  • 본 연구는 목저은 다음과 같다: 1) 돼지에서 150-kDa temary insulin-like growth faetor(IGF)complex의 한 구성 요소인 acid-labile subunit(ALS) 유전자 intron의 존재 확인. cloning 및 돼지 ALS(porcine ALS; pALS) complementary DNA(cDNA)의 3' 비해독(untranslated) 부위(3' UT) 증폭. cloning, 2) intron-spanning primer pair를 이용한 reverse transcription-polymerase chain reaction(RT-PCR) 방법에 의한 돼지 조직에서의 ALS 유전자 발현 분포 확인 및 3) 돼지 hepatocyte에서의 ALS 유전자 발현 여부 확인. 돼지 genomic DNA를 template로 하여 PCR 방법으로 예상된는 intron 부위를 증폭하고 plasmid vector에 삽입하여 염기서열을 결정한 결과 타 종의 ALS 유전자에서와 같은 위치에 1,371-base pair(bp)의 pALS intron이 존재함을 확인하였다. 역시 본 연구에서 간에서 추출한 RNA를 주형으로 시작하여 3' rapid amplification of cDNA end(3' RACE) 방법으로 147-bp의 3'UT를 합성하고 그 염기성열을 결정하였다. RT-PCR 결과 간은 물론 조사된 모든 돼지의 내장기관(신장, 폐, 비장)과 자성 생식기관(난소, 난관, 자궁) 및 골격근육에서 ALS 유전자가 발현됨이 밝혀졌다. 또한 돼지 간 조직에 대한 in-situ hybridization 결과 hepatocyte에서 ALS 유전자가 발현됨이 확인되었다. 이상의 결과는 ALS가 혈중 IGF의 저정/조절체로서의 주기능 외에 모세혈관 밖에서도 미지의 기능이 있을 기능성을 시사한다.

N2O 주개 리간드와 테레프탈레이트를 포함하는 구리(II) 착물의 용매를 매개로 한 수소결합형 초분자 네트워크 (Solvent Mediated Hydrogen-bonded Supramolecular Network of a Cu(II) Complex Involving N2O Donor Ligand and Terephthalate)

  • Chakraborty, Jishnunil
    • 대한화학회지
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    • 제55권2호
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    • pp.199-203
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    • 2011
  • 일차원 수소결합형 배위고분자 $[Cu^{II}(C_{13}H_{17}N_3OBr)(C_8H_5O_4)]{\cdot}2H_2O.CH_3OH$을 합성하여 단결정 X-선 회절 연구로 특성을 규명하였다. 단량체 단위는 사각평면의 중심 $Cu^{II}$를 갖고 있다. 네개의 배위자리 중 세자리는 $N_2O$-주개 세트를 갖는 Schiff 염기형 리간드 (4-bromo-2-[(2-piperazin-1-yl-ethylimino)-methyl]-phenol)가 차지하고, 네 번째 자리는 옆에 있는 테레프탈레이트 단위의 산소 원자가 차지한다. 두개의 인접한 중성분자는 분자간 N-H---O 및 O-H---N 수소결합에 의해 연결되어 이합체 쌍을 형성한다. 각 이합체 쌍은 불연속적인 물 및 메탄올 분자에 의해 수소결합으로 다시 연결되어 일차원 초분자 네트워크를 형성한다.

Clinical Features, Response to Treatment, Prognosis, and Molecular Characterization in Korean Patients with Inherited Urea Cycle Defects

  • Yoo, Han-Wook;Kim, Gu-Hwan;Seo, Eul-Ju
    • 대한유전성대사질환학회지
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    • 제2권1호
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    • pp.77-79
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    • 2002
  • The urea cycle, consisting of a series of six enzymatic reactions, plays key roles to prevent the accumulation of toxic nitrogenous compound and synthesize arginine de novo. Five well characterized diseases have been described, resulting from an enzymatic defect in the biosynthesis of one of the normally expressed enzyme. This presentation will focus on two representative diseases; ornithine transcarbamylase(OTC) deficiency and citrullinemia(argininosuccinate synthetase deficiency). OTC deficiency is one of the most common inborn error of urea cycle, which is inherited in X-linked manner. We identified 17 different mutations in 20 unrelated Korean patients with OTC deficiency; L9X, R26P, R26X, T44I, R92X, G100R, R141Q, G195R, M205T, H214Y, D249G, R277W, F281S, 853 del C, R320X, V323M and 10 bp del at nt. 796-805. These mutations occur at well conserved nucleotide sequences across species or CpG hot spot. The L9X and R26X lead to the disruption of leader sequences, required for directing mitochondrial localization of the OTC precursor. Their phenotypes are severe, and neonatal onset. The G100R, R277W and V323M mutations were uniquely identified in patients with late onset OTC deficiency. The other genotypes are associated with neonatal onset. Out of 20 patients with OTC deficiency, only 6 patients are alive; two were liver transplanted, and normal in growth and development at 2, 4 years after transplantation respectively. Citrullinemia is an autosomal recessive disease, caused by the mutations in the argininosuccinate synthetase(ASS) gene. We identified in 3 major mutations in 11 unrelated Korean patients with citrullinemia; G324S, $IVS6^{-2}$ A to G, and 67 bp ins at nt 1125-1126. Among these, the 67 base pair insertion mutation is novel. The allele frequency of each mutation is; G324S(45%), IVS6-2 A to G(32%), and 67 base pair insertion(14%). All patients are diagnosed at neonatal or infantile age. Interestingly, two patients presented with stroke like episode. Out of 11 patients, 5 patients died. Among 6 patients alive, one patient was successfully liver transplanted.

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가열산화 대두유의 돌연변이원성 (Mutagenicity of Thermally Oxidized Soybean Oil)

  • 이진영;안명수
    • 한국식품과학회지
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    • 제32권6호
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    • pp.1213-1220
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    • 2000
  • 가열산화된 유지의 산패도와 돌연변이원성과의 관련성을 알아보고자 대두유를 $180{\pm}3^{\circ}C$에서 48시간 동안 가열산화시키면서 0, 8, 16, 24, 32, 40, 48시간 후의 유지시료와 여기서 분획한 산화물 및 비산화물을 대상으로 Salmonella typimurium 변이균주 중 구조이동형(frame shift) 변이균주인 TA98, TA1537과 염기치환형(base-pair substituent) 변이균주인 TA100, TA1535 및 TA102를 사용하여 Ames test를 수행한 결과는 다음과 같다. $180{\pm}3^{\circ}C$에서 48시간 가열산화된 대두유의 가열시간에 따른 돌연변이원성을 조사했을 때 직접법 [-S9] 및 대사활성화법 [+S9] 모두에서 돌연변이원성이 인정되지 않았다. 또한 $180{\pm}3^{\circ}C$에서 가열산화된 대두유를 산화물 및 비산화물로 분리하여 돌연변이원성을 조사한 결과 가열 16시간 및 24시간 후의 산화 물질에서 돌연변이 활성이 나타났다.

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