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http://dx.doi.org/10.14405/kjvr.2019.59.2.69

Molecular characterization of Plasmodium juxtanucleare in Thai native fowls based on partial cytochrome C oxidase subunit I gene  

Pohuang, Tawatchai (Department of Veterinary Medicine, Faculty of Veterinary Medicine, Khon Kaen University)
Junnu, Sucheeva (Department of Veterinary Medicine, Faculty of Veterinary Medicine, Khon Kaen University)
Publication Information
Korean Journal of Veterinary Research / v.59, no.2, 2019 , pp. 69-74 More about this Journal
Abstract
Avian malaria is one of the most important general blood parasites of poultry in Southeast Asia. Plasmodium (P.) juxtanucleare causes avian malaria in wild and domestic fowl. This study aimed to identify and characterize the Plasmodium species infecting in Thai native fowl. Blood samples were collected for microscopic examination, followed by detection of the Plasmodium cox I gene by using PCR. Five of the 10 sampled fowl had the desired 588 base pair amplicons. Sequence analysis of the five amplicons indicated that the nucleotide and amino acid sequences were homologous to each other and were closely related (100% identity) to a P. juxtanucleare strain isolated in Japan (AB250415). Furthermore, the phylogenetic tree of the cox I gene showed that the P. juxtanucleare in this study were grouped together and clustered with the Japan strain. The presence of P. juxtanucleare described in this study is the first report of P. juxtanucleare in the Thai native fowl of Thailand.
Keywords
fowl; cytochrome C oxidase subunit I; Plasmodium juxtanucleare;
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  • Reference
1 Ruff MD. Important parasites in poultry production systems. Vet Parasitol 1999;84:337-347.   DOI
2 Williams RB. Avian malaria: clinical and chemical pathology of Plasmodium gallinaceum in the domesticated fowl Gallus gallus. Avian Pathol 2005;34:29-47.   DOI
3 Taylor MA, Coop RL, Wall RL. Parasites of Poultry and Gamebirds. In: Taylor MA, Coop RL, Wall RL (eds.). Veterinary Parasitology. 3rd ed. pp. 459-557, Blackwell Publishing Ltd., Oxford, 2007.
4 Versiani V, Gome BF. Sobre um novo hematozoario da galinha - "Plasmodium juxtanucleare n. sp. (Nota previa). Rev Bras Biol 1941;1:231-233.
5 Fernando MA, Dissanaike AS. Studies on Plasmodium gallinaceum and Plasmodium juxtanucleare from the Malayan jungle fowl Gallue gallus spadiceus. Southeast Asian J Trop Med Public Health 1975;6:25-32.
6 Kissinger JC, Souza PC, Soarest CO, Paul R, Wahl AM, Rathore D, McCutchan TF, Krettli AU. Molecular phylogenetic analysis of the avian malarial parasite Plasmodium (Novyella) juxtanucleare. J Parasitol 2002;88:769-773.   DOI
7 Tattiyapong M, Deemagarn T, Mohkeaw K. Molecular characterization of Plasmodium juxtanucleare in Burmese red junglefowls (Gallus gallus spadiceus) in Thailand. J Protozool Res 2016;26:1-10.
8 Pattaradilokrat S, Tiyamanee W, Simpalipan P, Kaewthamasorn M, Saiwichai T, Li J, Harnyuttanakorn P. Molecular detection of the avian malaria parasite Plasmodium gallinaceum in Thailand. Vet Parasitol 2015;210:1-9.   DOI
9 Joseph JT, Aldritt SM, Unnasch T, Puijalon O, Wirth DF. Characterization of a conserved extrachromosomal element isolated from the avian malarial parasite Plasmodium gallinaceum. Mol Cell Biol 1989;9:3621-3629.   DOI
10 Valkiunas G, Iezhova TA, Loiseau C, Smith TB, Sehgal RN. New malaria parasites of the subgenus Novyella in African rainforest birds, with remarks on their high prevalence, classification and diagnostics. Parasitol Res 2009;104:1061-1077.   DOI
11 Ekala MT, Khim N, Legrand E, Randrianarivelojosia M, Jambou R, Fandeur T, Menard D, Assi SB, Henry MC, Rogier C, Bouchier C, Mercereau-Puijalon O. Sequence analysis of Plasmodium falciparum cytochrome b in multiple geographic sites. Malar J 2007;6:164.   DOI
12 Perkins SL. Molecular systematics of the three mitochondrial protein-coding genes of malaria parasites: corroborative and new evidence for the origins of human malaria. Mitochondrial DNA 2008;19:471-478.
13 Farrugia C, Cabaret O, Botterel F, Bories C, Foulet F, Costa JM, Bretagne S. Cytochrome b gene quantitative PCR for diagnosing Plasmodium falciparum infection in travelers. J Clin Microbiol 2011;49:2191-2195.   DOI
14 Hellgren O, Waldenstrom J, Bensch S. A new PCR assay for simultaneous studies of Leucocytozoon, Plasmodium, and Haemoproteus from avian blood. J Parasitol 2004;90:797-802.   DOI
15 Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 1999;41:95-98.
16 Atkinson CT. Avian Malaria. In: Atkinson CT, Thomas NJ, Hunter DB (eds.). Parasitic Diseases of Wild Birds. pp. 35-53, Wiley-Blackwell Publishing, Iowa, 2008.
17 Omori S, Sato Y, Isobe T, Yukawa M, Murata K. Complete nucleotide sequences of the mitochondrial genomes of two avian malaria protozoa, Plasmodium gallinaceum and Plasmodium juxtanucleare. Parasitol Res 2007;100:661-664.   DOI
18 Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 1994;22:4673-4680.   DOI
19 Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013;30:2725-2729.   DOI
20 Murata K, Nii R, Sasaki E, Ishikawa S, Sato Y, Sawabe K, Tsuda Y, Matsumoto R, Suda A, Ueda M. Plasmodium (Bennettinia) juxtanucleare infection in a captive white earedpheasant (Crossoptilon crossoptilon) at a Japanese zoo. J Vet Med Sci 2008;70:203-205.   DOI
21 Bennett GF, Bishop MA, Peirce MA. Checklist of the avian species of Plasmodium Marchiafava and Celli, 1885 (Apicomplexa) and their distribution by avian family and Wallacean life zones. Syst Parasitol 1993;26:171-179.   DOI