• Animal Systematics, Evolution and Diversity
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• v.36 no.2
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• pp.182-184
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• 2020

Isaacsicalanus paucisetus Fleminger, 1983, a monotypic species of the family Spinocalanidae Vervoort, 1951, was first reported from a hydrothermal vent field in the East Pacific Rise off the mouth of the Gulf of California. The mitochondrial cytochrome oxidase I(mtCOI) DNA barcodes are considered a useful tool to assist traditional taxonomy and species discrimination in calanoid copepods. However, the mtCOI DNA barcodes of I. paucisetus have not been reported due to the species rarity and the difficulty of sampling. In this study, we firstly determined the mtCOI DNA barcodes of the I. paucisetus newly collected from a hydrothermal vent in the North Fiji Basin of the southwestern Pacific. All mtCOI DNA barcodes of I. paucisetus were identical and intraspecies variations of spinocalanid species were 0.0-3.0%. Interspecies and intergeneric variations were 13.4-25.2% and 16.7-24.1%, respectively. The DNA barcodes of I. paucisetus obtained in the present study would be helpful for understanding taxonomic relationships of widespread spinocalanid species.

• Genomics & Informatics
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• v.16 no.2
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• pp.22-29
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• 2018

Incorporation of unique barcodes into fission yeast gene deletion collections has enabled the identification of gene functions by growth fitness analysis. For fine tuning, it is important to examine barcode sequences, because mutations arise during strain construction. Out of 8,708 barcodes (4,354 strains) covering 88.5% of all 4,919 open reading frames, 7,734 barcodes (88.8%) were validated as high-fidelity to be inserted at the correct positions by Sanger sequencing. Sequence examination of the 7,734 high-fidelity barcodes revealed that 1,039 barcodes (13.4%) deviated from the original design. In total, 1,284 mutations (mutation rate of 16.6%) exist within the 1,039 mutated barcodes, which is comparable to budding yeast (18%). When the type of mutation was considered, substitutions accounted for 845 mutations (10.9%), deletions accounted for 319 mutations (4.1%), and insertions accounted for 121 mutations (1.6%). Peculiarly, the frequency of substitutions (67.6%) was unexpectedly higher than in budding yeast (~28%) and well above the predicted error of Sanger sequencing (~2%), which might have arisen during the solid-phase oligonucleotide synthesis and PCR amplification of the barcodes during strain construction. When the mutation rate was analyzed by position within 20-mer barcodes using the 1,284 mutations from the 7,734 sequenced barcodes, there was no significant difference between up-tags and down-tags at a given position. The mutation frequency at a given position was similar at most positions, ranging from 0.4% (32/7,734) to 1.1% (82/7,734), except at position 1, which was highest (3.1%), as in budding yeast. Together, well-defined barcode sequences, combined with the next-generation sequencing platform, promise to make the fission yeast gene deletion library a powerful tool for understanding gene function.

• Journal of the Korean Society for Library and Information Science
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• v.44 no.2
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• pp.309-331
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• 2010

In this study, the characteristics and types of 1D, 2D barcodes were examined. It is necessary to provide mobile services by making full use of the advantages of the technologies, such as 1D, 2D barcodes, and RFID, and by complementing the disadvantages of them mutually in Korea. Especially, in a mobile environment where the spread of smart phones and mobile networks are expanding, barcodes and RFID (Radio-Frequency Identification) were analyzed as a very useful information media which is able to connect offline analogue media and online digital media. In addition, library automation systems and the cases of mobile student ID services using barcodes and RFID in Korean libraries were analyzed. The cases of mobile services using barcodes in foreign countries were also analyzed. With these cases, we propose a method to empower mobile services using barcodes in Korean libraries.

• Journal of Korea Society of Digital Industry and Information Management
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• v.8 no.3
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• pp.65-74
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• 2012

A barcode is a representative means of cognition. It is either printed on the package of a product or attached to it as a sticker. It is used for the fast cognition of a product at a store. It is considerably cheap to make a barcode. Also, it is possible to read it fast by using a barcode reader. Because of such convenience provided by the barcode, a new system using the barcode as a means of settling payments like a currency or a credit card has been developed. However, due to its characteristics, it is easy to reduplicate, forge or falsify a barcode easily. Therefore, this study focuses on the case of applying the system using barcodes as a means of settling payments without providing solutions for the potential weaknesses. Also, this study suggests various points to consider regarding the creation of safe barcodes as one of the related measures, while providing various methods using additional means of certification other than the one of using barcodes in addition to the way of applying complexity with barcode numbers. Throughout this study, it will be possible to safely establish and operate the payment-settlement system using barcodes.

• ETRI Journal
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• v.29 no.2
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• pp.189-200
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• 2007

This paper addresses the localization and navigation problem in the movement of service robots by using invisible two dimensional barcodes on the floor. Compared with other methods using natural or artificial landmarks, the proposed localization method has great advantages in cost and appearance since the location of the robot is perfectly known using the barcode information after mapping is finished. We also propose a navigation algorithm which uses a topological structure. For the topological information, we define nodes and edges which are suitable for indoor navigation, especially for large area having multiple rooms, many walls, and many static obstacles. The proposed algorithm also has the advantage that errors which occur in each node are mutually independent and can be compensated exactly after some navigation using barcodes. Simulation and experimental results were performed to verify the algorithm in the barcode environment, showing excellent performance results. After mapping, it is also possible to solve the kidnapped robot problem and to generate paths using topological information.

• Animal Systematics, Evolution and Diversity
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• v.35 no.1
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• pp.30-32
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• 2019

The brachyuran crab Bythograeidae Williams, 1980 is common in hydrothermal vent fields worldwide and has recorded to sixteen species of six genera. In this study, we firstly determined the cytochrome c oxidase subunit 1 (CO1) DNA barcodes for the fifth species of Austinograea, A. hourdezi, from hydrothermal vent regions of the North Fiji Basin in southwestern Pacific Ocean. All CO1 DNA barcodes of A. hourdezi were identical. The interspecies variations of three bythograeid genera were 10.9-13.3% for Austinograea, 6.6-15.7% for Bythograea, and 9.7% for Gandalfus. These results would be helpful to understand taxonomy of brachyuran crabs living in hydrothermal vent fields using CO1 DNA barcodes.

• The Plant Pathology Journal
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• v.40 no.2
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• pp.171-191
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• 2024

Identification of Helicotylenchus species is very challenging due to phenotypic plasticity and existence of cryptic species complexes. Recently, the use of rDNA barcodes has proven to be useful for identification of Helicotylenchus. Molecular markers are a quick diagnostic tool and are crucial for discriminating related species and resolving cryptic species complexes within this speciose genus. However, DNA barcoding is not an error-free approach. The public databases appear to be marred by incorrect sequences, arising from sequencing errors, mislabeling, and misidentifications. Herein, we provide a comprehensive analysis of the newly obtained, and published DNA sequences of Helicotylenchus, revealing the potential faults in the available DNA barcodes. A total of 97 sequences (25 nearly full-length 18S-rRNA, 12 partial 28S-rRNA, 16 partial internal transcribed spacer [ITS]-rRNA, and 44 partial cytochrome c oxidase subunit I [COI] gene sequences) were newly obtained in the present study. Phylogenetic relationships between species are given as inferred from the analyses of 103 sequences of 18S-rRNA, 469 sequences of 28S-rRNA, 183 sequences of ITS-rRNA, and 63 sequences of COI. Remarks on suggested corrections of published accessions in GenBank database are given. Additionally, COI gene sequences of H. dihystera, H. asiaticus and the contentious H. microlobus are provided herein for the first time. Similar to rDNA gene analyses, the COI sequences support the genetic distinctness and validity of H. microlobus. DNA barcodes from type material are needed for resolving the taxonomic status of the unresolved taxonomic groups within the genus.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.23 no.4
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• pp.805-814
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• 1998

An efficient image processing algorithm is proposed to recognize both the height and width modulated barcodes which are rotated and printed at an arbitrary position. The main feature of this algorithm is to utilize the gradient information of a rotated barcode with a Sobel operator. The barcode area is extracted using the gradient information, and the barcode is decoded from the binary image of the extracted area. Theis algorithm is successfully applied to the 4 state and width modulated barcodes. It takes 0.86 secoden to process a letter, and the recognition rate reaches above 98% under various testing conditions. Since both the width and height modulated barcodes are processed with the proposed algorithm, it can be applied to postal service automation.

• Journal of KIISE
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• v.42 no.7
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• pp.868-877
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• 2015

The barcode area extraction from images has been extensively studied, and existing methods exploit frequency characteristics or depend on the Hough transform (HT). However, the slantedness of the images and noise affects the performance of these approaches. Moreover, it is difficult to deal with the case where an image contains multiple barcodes. We therefore propose a barcode detection algorithm that is robust under such unfavorable conditions. The pre-processing step implements a probabilistic Hough transform to determine the areas that contain barcodes with a high probability, regardless of the slantedness, noise, and the number of instances. Then, a frequency component analysis extracts the barcodes. We successfully implemented the proposed system and performed a series of barcode extraction tests.

• Korean Journal of Plant Resources
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• v.35 no.2
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• pp.346-361
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• 2022

Food poisoning accidents caused by poisonous plants occur every year. As certain poisonous plants are mistaken for edible plants causing food poisoning, accurate species identification of poisonous plants is required. DNA barcodes suitable for species identification of poisonous plants and database that can be used for accurate species identification are necessary for their use in forensic cases. In this study, species identification of 19 poisonous plants native to Jeju Island using seven DNA barcodes (trnH-psbA, trnL-trnF, trnL intron, rbcL, matK, ITS1-ITS4, 18S rRNA) was performed to construct a database containing sequence information and DNA barcode universality. trnL-trnF barcode and ITS1-ITS4 barcode were the easiest markers for PCR amplification and sequence retrieval, and the combination of the two barcodes enabled single species identification in 18 out of 19 plants. Therefore, when an investigation of unknown poisonous plants is requested, combination of trnL-trnF and ITS1-ITS4 barcodes is considered as a primary marker for species identification. The database of recommended DNA barcodes for each poisonous plant presented in this study will be helpful in plants poisoning cases.