• 지질공학
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• 제28권1호
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• pp.101-115
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• 2018

본 연구는 자유면대수층 즉, 충적대수층에서 다양한 필터조건에 따른 영향을 평가하기 위하여 수행하였다. 시험을 위해 모래층 및 자갈층으로 구성된 충적대수층에 필터층, 양수정, 관측정을 각각 설치한 모형시험장치를 제작하였다. 제작된 모형시험장치를 이용해 단계 및 장기양수시험을 수행하였으며, 각 시험조별 투수특성을 확인하고 적정양수량을 산정하였다. 모래층에서 수행한 이중필터와 단일필터의 적정양수량을 비교하면, 이중필터인 SD-300은 동일두께의 단일필터인 SS-300에 비해 최종 적정양수량이 216.8% 높게 평가되었다. 이중필터 SD-300과 필터층 두께가 얇은 단일필터 SS-100을 비교하면, 이중필터인 SD-300은 SD-100에 비해 최종 적정양수량이 709.2% 높게 나타났다. 시간경과에 따른 적정양수량의 변화를 분석한 결과, 이중필터와 단일필터의 적정양수량 비는 시간이 경과할수록 증대됨을 확인하였다. 장기적인 취수효율의 변화를 평가하기 위하여 초기 적정양수량이 50% 저감되는 시점을 검토한 결과, 이중필터인 SD-300은 동일두께 단일필터인 SS-300 대비 약 351.1% 높은 값을 보이며, SS-100 대비 약 579.0% 높은 값을 보인다. 이러한 결과를 볼 때, 초기 양수량이 50%로 저하되는 지점을 우물 수명으로 가정하면, 이중필터는 동일두께의 단일필터 대비 약 3.5배, 일반적인 단일필터 대비 약 5.8배의 수명 증대가 예상된다. 이와 같은 결과는 강변여과수나 해변여과수를 활용하기 위한 우물설계에 활용될 수 있을 것이다. 또한, 향후 수행될 현장실증시험 성과와의 상호비교를 통해 이중필터 효과에 대한 보다 명확한 규명이 가능할 것으로 판단된다.

• Journal of Microbiology and Biotechnology
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• 제21권6호
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• pp.574-581
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• 2011

A Gram-positive bacterium was isolated from the saline soils of Jangpura (U.P.), India, and showed high-level of radiation-resistant property and survived upto 12.5 kGy dose of gamma radiation. The 16S rDNA sequence of this strain was examined, identified as Bacillus sp. strain HKG 112, and was submitted to the NCBI GenBank (Accession No. GQ925432). The mechanism of radiation resistance and gene level expression were examined by proteomic analysis of whole-cell extract. Two proteins, 38 kDa and 86.5 kDa excised from SDS-PAGE, which showed more significant changes after radiation exposure, were identified by MALDI-TOF as being flagellin and S-layer protein, respectively. Twenty selected 2-DE protein spots from the crude extracts of Bacillus sp. HKG 112, excised from 2- DE, were identified by liquid chromatography mass spectrometry (LC-MS) out of which 16 spots showed significant changes after radiation exposure and might be responsible for the radiation resistance property. Our results suggest that the different responses of some genes under radiation for the expression of radiation-dependent proteins could contribute to a physiological advantage and would be a significant initial step towards a fullsystem understanding of the radiation stress protection mechanisms of bacteria in different environments.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2010년도 춘계학술대회 초록집
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• pp.172.2-172.2
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• 2010

This study was carried out in order to reduce the installation expense of heating system for greenhouse comparing to geothermal heat pump and develope the coefficient of performance (COP) for a heat pump. For getting plenty of heat flux from geothermal energy. Surface water in river channel was used for getting a lots of geothermal heat by penetrating water through underground soil layer of the river bank that make heat transmission to passing water. The range of water temperature after the process of Ground filtration is 13~18 degrees celsius which is very similar to low heat source of geothermal heat pump system and the plenty amount of heat source from that make the number of geothermal heat exchanging hole and the expense for geothermal heat exchanger construction reduced. Drainage well is also used for returning filtration water to the aquifer that keep the water good recirculation from losing geothermal heat and water resource. For the COP improvement of Heat pump, thermal storage tank with separating insulation plate according to the temperature difference make the COP of Heat pump that is similar to thermal storage tank with diffuser. Developed thermal storage tank make construction expense cheaper than customarily used one's. and that sand filter and oxidation sand (FELOX) are going to be used for improving ground filtration water quality that make heat exchanger efficiency better. All above developed component skill are going to be set on the Ground filtration water source heat pump system and applied for medium, large scale for protected greenhouse in riverside area and on-site experiment is going to do for optimizing the heating system function and overcome the problem happening in the process of on-site application afterward.

• 디지털콘텐츠학회 논문지
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• 제18권1호
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• pp.183-190
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• 2017

본 논문에서는 깊은 신경망을 이용한 오디오 이벤트 검출 방법을 제안한다. 오디오 입력의 매 프레임에 대한 오디오 이벤트 확률을 feed-forward 신경망을 적용하여 생성한다. 매 프레임에 대하여 멜 스케일 필터 뱅크 특징을 추출한 후, 해당 프레임의 전후 프레임으로부터의 특징벡터들을 하나의 특징벡터로 결합하고 이를 feed-forward 신경망의 입력으로 사용한다. 깊은 신경망의 출력층은 입력 프레임 특징값에 대한 오디오 이벤트 확률값을 나타낸다. 연속된 5개 이상의 프레임에서의 이벤트 확률값이 임계값을 넘을 경우 해당 구간이 오디오 이벤트로 검출된다. 검출된 오디오 이벤트는 1초 이내에 동일 이벤트로 검출되는 동안 하나의 오디오 이벤트로 유지된다. 제안된 방법으로 구현된 오디오 이벤트 검출기는 UrbanSound8K와 BBC Sound FX자료에서의 20개 오디오 이벤트에 대하여 71.8%의 검출 정확도를 보였다.

• 대한전자공학회논문지SP
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• 제40권6호
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• pp.106-113
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• 2003

Modified Discrete Cosine Transform (MDCT)와 역변환인 IMDCT는 서브밴드 및 변환 코딩 기법에서 시간 영역 에일리어징 제거(Time Domain Aliasing Cancellation)를 기반으로 하는 분석/합성 필터 뱅크로서 채택되고 있으며, MPEG 오디오 표준의 레이어-Ⅲ에서 가장 많은 연산 량을 필요로 한다 본 논문에서는 MDCT/IMDCT를 효율적으로 계산할 수 있는 새로운 알고리즘을 제안하였다. 제안한 알고리즘은 DCT를 이용하여 MDCT/IMDCT를 계산하는 알고리즘에 기반을 두고 있기 때문에, MDCT/IMDCT 계산을 위해 두 개의 DCT-Ⅱ를 이용한다. 더불어, 제안한 알고리즘은 4로 나누어지는 길이의 입력을 갖는 MDCT/IMDCT의 계산에도 적용할 수 있다. 제안한 알고리즘은 계산 복잡도 면에서 기존의 알고리즘들과 비교하여 적은 계산 량을 필요로 하며, 구조적인 면에서 병렬적인 구조로 나타낼 수 있기 때문에, VLSI 구현에 매우 적합하다.

• The Korean Journal of Physiology and Pharmacology
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• 제14권5호
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• pp.265-272
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• 2010

Follicular cystic ovary (FCO) is one of the most frequently diagnosed ovarian diseases and is a major cause of reproductive failure in mammalian species. However, the mechanism by which FCO is induced remains unclear. Genetic alterations which affect the functioning of many kinds of cells and/or tissues could be present in cystic ovaries. In this study, we performed a comparison analysis of gene expression in order to identify new molecules useful in discrimination of bovine FCO with follicular cystic follicles (FCFs). Normal follicles and FCFs were classified based on their sizes (5 to 10 mm and $\geq25mm$). These follicles had granulosa cell layer and theca interna and the hormone $17{\beta}$-estradiol ($E_2$)/ progesterone ($P_4$) ratio in follicles was greater than one. Perifollicular regions including follicles were used for the preparation of RNA or protein. Differentially expressed genes (DEG) that showed greater than a 2-fold change in expression were screened by the annealing control primer (ACP)-based PCR method using $GeneFishing^{TM}$ DEG kits in bovine normal follicles and FCFs. We identified two DEGs in the FCFs: ribosomal protein L15 (RPL15) and microtubule-associated protein 1B (MAP1B) based on BLAST searches of the NCBI GenBank. Consistent with the ACP analysis, semi-quantitative PCR data and Western blot analyses revealed an up-regulation of RPL15 and a down-regulation of MAP1B in FCFs. These results suggest that RPL15 and MAP1B may be involved in the regulation of pathological processes in bovine FCOs and may help to establish a bovine gene data-base for the discrimination of FCOs from normal ovaries.

• Journal of Information Processing Systems
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• 제9권4호
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• pp.633-650
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• 2013

All the imputation techniques proposed so far in literature for data imputation are offline techniques as they require a number of iterations to learn the characteristics of data during training and they also consume a lot of computational time. Hence, these techniques are not suitable for applications that require the imputation to be performed on demand and near real-time. The paper proposes a computational intelligence based architecture for online data imputation and extended versions of an existing offline data imputation method as well. The proposed online imputation technique has 2 stages. In stage 1, Evolving Clustering Method (ECM) is used to replace the missing values with cluster centers, as part of the local learning strategy. Stage 2 refines the resultant approximate values using a General Regression Neural Network (GRNN) as part of the global approximation strategy. We also propose extended versions of an existing offline imputation technique. The offline imputation techniques employ K-Means or K-Medoids and Multi Layer Perceptron (MLP)or GRNN in Stage-1and Stage-2respectively. Several experiments were conducted on 8benchmark datasets and 4 bank related datasets to assess the effectiveness of the proposed online and offline imputation techniques. In terms of Mean Absolute Percentage Error (MAPE), the results indicate that the difference between the proposed best offline imputation method viz., K-Medoids+GRNN and the proposed online imputation method viz., ECM+GRNN is statistically insignificant at a 1% level of significance. Consequently, the proposed online technique, being less expensive and faster, can be employed for imputation instead of the existing and proposed offline imputation techniques. This is the significant outcome of the study. Furthermore, GRNN in stage-2 uniformly reduced MAPE values in both offline and online imputation methods on all datasets.

• Mycobiology
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• 제48권5호
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• pp.427-430
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• 2020

Ganoderma lingzhi is a well-known source of natural fungal medicines which has been given for the treatment of several diseases. China is one of the major commercial producers of Ganoderma mushroom worldwide. However, with the expansion of the commercial cultivation, the occurrence of the fungal diseases on G. lingzhi has also been increased. The green mold disease symptoms were observed in the cultivation base of G. lingzhi in Zuojia Town, Jilin City, Jilin Province, China, causing the basidiomes to be rotten and withered, and the green mycelium layer generated gradually. The pathogenicity tests showed the same symptoms as appeared naturally in Zuojia mushroom base. Morphology characters revealed conidia green, ellipsoid, globose, 2.56-4.83 × 2.09-4.22 ㎛, length-width ratio was 1.1-1.2 (n = 10). Conidiophores trichoderma-like, often asymmetry, branches solitary, paired or in whorls of 3 phialides formed solitary, paired or in whorl, variable in shape, lageniform, sometimes ampulliform or subulate. While using molecular methodology, comparing with the sequences of Trichoderma hengshanicum from GenBank, the analyzed sequence showed 97.32% homology with the RPB2 sequences, 100% with the TEF1-a sequences. A fungus isolated from the diseased tissues was identified based on morphology and molecular studies as T. hengshanicum. This is the first report of T. hengshanicum causing the green mold disease of G. lingzhi in China.

• Molecules and Cells
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• 제44권10호
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• pp.736-745
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• 2021

Although various marine ingredients have been exploited for the development of cosmetic products, no previous study has examined the potential of seaweed extracellular vesicles (EV) in such applications. Our results revealed that EV from Codium fragile and Sargassum fusiforme effectively decreased α-MSH-mediated melanin synthesis in MNT-1 human melanoma cells, associated with downregulation of MITF (microphthalmia-associated transcription factor), tyrosinase and TRP1 (tyrosinase-related proteins 1). The most effective inhibitory concentrations of EV were 250 ㎍/ml for S. fusiforme and 25 ㎍/ml for C. fragile, without affecting the viability of MNT-1 cells. Both EV reduced melanin synthesis in the epidermal basal layer of a three-dimensional model of human epidermis. Moreover, the application of the prototype cream containing C. fragile EV (final 5 ㎍/ml) yielded 1.31% improvement in skin brightness in a clinical trial. Together, these results suggest that EV from C. fragile and S. fusiforme reduce melanin synthesis and may be potential therapeutic and/or supplementary whitening agents.

• 한국지형학회지
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• 제27권2호
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• pp.29-46
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• 2020

In this study, changes in the fluvial landforms of the Yeoju section of the Han River, which was made up of the Han River Restoration Project, were examined through existing previous research data, government's environmental impact assessment data, satellite images, and field observations. For example, In the vicinity of Dori Island, the most upstream part of the study section, the location of the confluence of the Han River and Cheongmi Stream was changed, and it was found that a significant portion of the sand sedimentary layer disappeared. In the Bawuinupgubi area, the wetland, which is the first class in the ecological nature, was greatly modified, and the elevation of the ground rose as Gangcheon island and it was completely separated from the river by dredging The confluence of Geumdangcheon and the point bar of Yeonyang-ri in the south were also dredged, turned into an artificial waterfront park, and a chute channel remained in the form of a wetland was also developed as a recreational park. The deposional forms around Baekseok-ri islands also disappeared as dredging was carried out. Among the areas adjacent to the confluence of Bokcheon and Yangchon-ri Island, some sedimentay forms remains, but the abandonned channel between Yangchon-ri and the northern river bank has been changed into a riverside reservoir through dredging and embankment construction, and the waterway of the tributary river(Yazoo) has been greatly changed.