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http://dx.doi.org/10.14348/molcells.2021.2167

Extracellular Vesicles from Korean Codium fragile and Sargassum fusiforme Negatively Regulate Melanin Synthesis  

Jang, Bohee (Department of Life Sciences, The Research Center for Cellular Homeostasis, Ewha Womans University)
Chung, Heesung (Department of Life Sciences, The Research Center for Cellular Homeostasis, Ewha Womans University)
Jung, Hyejung (Department of Life Sciences, The Research Center for Cellular Homeostasis, Ewha Womans University)
Song, Hyun-Kuk (Department of Life Sciences, The Research Center for Cellular Homeostasis, Ewha Womans University)
Park, Eunhye (Department of Life Sciences, The Research Center for Cellular Homeostasis, Ewha Womans University)
Choi, Hack Sun (Subtropical/Tropical Organism Gene Bank, Jeju National University)
Jung, Kyuhyun (ExoMed, Inc.)
Choe, Han (Department of Physiology, University of Ulsan College of Medicine, Asan Medical Center)
Yang, Sanghwa (ExoMed, Inc.)
Oh, Eok-Soo (Department of Life Sciences, The Research Center for Cellular Homeostasis, Ewha Womans University)
Publication Information
Molecules and Cells / v.44, no.10, 2021 , pp. 736-745 More about this Journal
Abstract
Although various marine ingredients have been exploited for the development of cosmetic products, no previous study has examined the potential of seaweed extracellular vesicles (EV) in such applications. Our results revealed that EV from Codium fragile and Sargassum fusiforme effectively decreased α-MSH-mediated melanin synthesis in MNT-1 human melanoma cells, associated with downregulation of MITF (microphthalmia-associated transcription factor), tyrosinase and TRP1 (tyrosinase-related proteins 1). The most effective inhibitory concentrations of EV were 250 ㎍/ml for S. fusiforme and 25 ㎍/ml for C. fragile, without affecting the viability of MNT-1 cells. Both EV reduced melanin synthesis in the epidermal basal layer of a three-dimensional model of human epidermis. Moreover, the application of the prototype cream containing C. fragile EV (final 5 ㎍/ml) yielded 1.31% improvement in skin brightness in a clinical trial. Together, these results suggest that EV from C. fragile and S. fusiforme reduce melanin synthesis and may be potential therapeutic and/or supplementary whitening agents.
Keywords
extracellular vesicles; melanin synthesis; seaweed; skin epidermis;
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