• 생약학회지
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• 제42권2호
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• pp.175-181
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• 2011

Oxidative stress or accumulation of reactive oxygen species (ROS) leads neuronal cellular death and dysfunction, and it contributes to neuronal degenerative disease such as Alzheimer's disease, Parkinson's disease and stroke. Glutamate is one of the major excitatory neurotransmitter in the central nervous system (CNS). Glutamate contributes to fast synaptic transmission, neuronal plasticity, outgrowth and survival, behavior, learning and memory. In spite of these physiological functions, high concentration of glutamate causes neuronal cell damage, acute insults and chronic neuronal neurodegenerative diseases. Heme oxygenase-1 (HO-1) enzyme plays an important role of cellular antioxidant system against oxidant injury. NNMBS020, the water-insoluble fraction of the 70% EtOH extract of root barks of Dictamnus dasycarpus, showed dominant neuroprotective effects on glutamate-induced neurotoxicity in mouse hippocampal HT22 cells by induced the expression of HO-1 and increased HO activity. In mouse hippocampal HT22 cells, NNMBS020 makes the nuclear accumulation of Nrf2 and stimulates extracellular signal-regulated kinase (ERK) pathway. The ERK MAPK pathway inhibitor significantly reduced NNMBS020-induced HO-1 expression, whereas the JNK and p38 inhibitors did not. In conclusion, the water-insoluble fraction of the 70% EtOH extract of root barks of D. dasycarpus (NNMBS020) significantly protect glutamate-induced oxidative damage by induction of HO-1 via Nrf2 and ERK pathway in mouse hippocampal HT22 cells.

• Natural Product Sciences
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• 제18권3호
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• pp.177-182
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• 2012

Oxidative stress potentially induces neurotoxicity which is believed to underlie several major age-related diseases of the central nervous system. This study sought to identify the cytoprotective effects of sixty-nine Cambodian plants against glutamate-induced cell death. Cultured HT22 cells were applied as an in vitro model, and neurotoxicity was induced in these neuronal cells by exposure to a determined concentration of glutamate. Sixty-nine plant sources, as Cambodia's indigenous species, were purchased from O'reusey Market, Phnom Penh, and extracted with ethanol. These extracts were screened for cytoprotective effects against glutamate-triggered neurotoxicity in HT22 cells at concentrations of 100 and 300 ${\mu}g/ml$. Of these, eight ethanol extracts, bark of Anacardium occidentale, bark and sapwood of Bauhinia pulla, flowers of Borassus flabellifer, stems and leaves of Coix lacryma-jobi, bark and sapwood of Diospyros nitida, sapwood of Dipterocarpus obtusifolius, stems of Oryza rufipogon, and fruits of Phyllanthus emblica, showed significant cytoprotective effects against glutamate-induced cell damage and degeneration in HT22 cells.

• Journal of Ginseng Research
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• 제45권1호
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• pp.48-57
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• 2021

Background: Ginsenoside Rh2 is well known for many pharmacological activities, such as anticancer, antidiabetes, antiinflammatory, and antiobesity properties. Glycosyltransferases (GTs) are ubiquitous enzymes present in nature and are widely used for the synthesis of oligosaccharides, polysaccharides, glycoconjugates, and novel derivatives. We aimed to synthesize new ginsenosides from Rh2 using the recombinant GT enzyme and investigate its cytotoxicity with diverse cell lines. Methods: We have used a GT gene with 1,224-bp gene sequence cloned from Lactobacillus rhamnosus (LRGT) and then expressed in Escherichia coli BL21 (DE3). The recombinant GT protein was purified and demonstrated to transform Rh2 into two novel ginsenosides, and they were characterized by nuclear magnetic resonance (NMR) techniques and evaluated by 3-(4, 5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide assay. Results: Two novel ginsenosides with an additional glucopyranosyl (6→1) and two additional glucopyranosyl (6→1) linked with the C-3 position of the substrate Rh2 were synthesized, respectively. Cell viability assay in the lung cancer (A549) cell line showed that glucosyl ginsenoside Rh2 inhibited cell viability more potently than ginsenoside Rg3 and Rh2 at a concentration of 10 μM. Furthermore, glucosyl ginsenoside Rh2 did not exhibit any cytotoxic effect in murine macrophage cells (RAW264.7), mouse embryo fibroblasts cells (3T3-L1), and skin cells (B16BL6) at a concentration of 10 μM compared with ginsenoside Rh2 and Rg3. Conclusion: This is the first report on the synthesis of two novel ginsenosides, namely, glucosyl ginsenoside Rh2 and diglucosyl ginsenoside Rh2 from Rh2 by using recombinant GT isolated from L. rhamnosus. Moreover, diglucosyl ginsenoside Rh2 might be a new candidate for treatment of inflammation, obesity, and skin whiting, and especially for anticancer.

• Ecology and Resilient Infrastructure
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• 제6권4호
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• pp.217-226
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• 2019

본 연구에서는 하천의 하안 경사와 친수시설 유무에 따라 적절한 하안 보호 공법을 유형화하였으며, 특히 기존의 호안 공법으로 하안침식 방지 대책 적용이 적절하지 않은 유형에 대해 적용 가능한 신개념 Eco-hybrid 롤링매트 공법을 제시하였다. Eco-hybrid 롤링매트 공법은 지반과 사면의 교란없이 하천 환경과 생태 조건의 변화를 최소화하는 시공이 가능하고, 하안침식이 발생할 경우 즉각적인으로 호안이나 제방 사면을 보호할 수 있는 경제적이고 효율적인 공법이다. 개발된 Eco-hybrid 롤링매트 공법은 실규모 하천 실험을 통해 공법의 구조 및 시스템, 기능과 효과를 검증하였다. 현장에서 발생할 수 있는 여러 변형 및 부패 테스트를 포함한 실규모 하천 실험 검증을 수행하였으며, 저유속과 고유속 조건에서 롤링매트의 작동과 하안침식 방지 효과를 확인하였다. 하안침식 방지 효과는 유사 농도 분석을 통해 정량적으로 검증하였으며, 실험에 활용한 롤링매트를 기준으로 규격화하여 Eco-hybrid 롤링매트 공법의 제품의 시방 (안)을 제시하였다.

• Parasites, Hosts and Diseases
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• 제61권1호
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• pp.60-71
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• 2023

Cockroaches can cause allergic sensitization in humans via contact with their feces or frass. Antibiotics can affect concentration of major allergen and total bacteria production in German cockroaches (Blattella germanica). This study examined the ability of antibiotic-treated German cockroaches to induce allergic airway inflammation and the effect of antibiotics on their lipopolysaccharide and Bla g1, 2, and 5 expression levels. Specifically, we measured the ability of German cockroach extract (with or without prior antibiotic exposure) to induce allergic inflammation in human bronchial epithelial cells and a mouse model of asthma. Bacterial 16S rRNA and lipopolysaccharide levels were lower in ampicillin-treated cockroaches than in the control group. The Bla g1, Bla g2, and Bla g5 expression in ampicillin-treated cockroaches decreased at both the protein and RNA levels. In human bronchial epithelial cell lines BEAS-2B exposed to the ampicillin-treated extract, expression levels of interleukin-6 and interleukin-8 were lower than that in the control group. The total cell count and eosinophil count in bronchoalveolar lavage fluid was also lower in mice exposed to the ampicillin-treated extract than in those exposed to normal cockroach extract. Mouse lung histopathology showed reduced immune cell infiltration and mucus production in the ampicillin group. Our results showed that ampicillin treatment reduced the symbiont bacterial population and major allergen levels in German cockroaches, leading to reduced airway inflammation in mice. These results can facilitate the preparation of protein extracts for immunotherapy or diagnostics applications.

• 문화기술의 융합
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• 제5권2호
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• pp.77-82
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• 2019

본 연구는 저축은행의 소유집중도와 사업다각화 간의 관계를 분석하고자 함이 목적이다. 패널데이터를 이용하여 저축은행의 소유집중도와 사업다각화의 관계를 분석한 것은 기존연구와의 차별성이라 할 수 있다. 분석기간은 2014년부터 2018년까지이며, 79개 저축은행을 대상으로 반기 재무자료를 이용하였다. 연구모형은 자기상관 문제를 고려한 확률효과 GLS(Random effects Generalized Linear Square)모형을 이용하여 분석하였다. 분석결과, 저축은행의 지배주주지분율과 사업다각화의 관계는 유의한 양(+)의 값을 가지는 것으로 추정되었다. 즉 지배주주지분율이 증가할수록 사업다각화를 대리하는 변수인 비이자수익률이 증가하는 것으로 나타났다. 이는 위험회피가설을 지지하는 결과이며, 지배주주지분율이 높을수록 사업다각화를 통해 자신의 자산위험과 고용위험을 축소시키기 위해 적극적인 다각화 전략을 추진하는 것으로 나타났다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제5권6호
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• pp.465-468
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• 2000

Methyl mercaptan oxidase was successfully induced from Rhodococcus rhodochrous IGTS8 using methyl mercaptan gas and purified to homogeneity for the detection of mercaptans. The purification procedure involved DEAE-Sephacel and Superose 12 column chromatography with recovery yields of 85.8 and 83.3%, and a specific activity of 92.7 and 303.4 units/mg-protein, respectively. The molecular weight of purified methyl mercaptan oxidase was determined to be 64.5 kDa by SDS-PAGE. The extract from gel filtration chromatography oxidizes methyl mercaptan to produce formaldehyde, which can be easily detected by the purpald-coloring method. Optimum temperature for activity was achieved at 60$^{\circ}C$. This enzyme was inhibited by both K$_2$SO$_4$and NaCl at concentration of less than 100mM and recovered to original activity at concentration of 200mM. In the presence of methanol, the activity decreased by 33%.

• 생태와환경
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• 제41권spc호
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• pp.1-10
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• 2008

We patterned water quality of agricultural reservoirs according to the differences of six physico-chemical environmental factors (TN, TP, DO, BOD, COD, and SS) using four different ordination methods: Principal Components Analysis (PCA), Detrended Correspondence Analysis (DCA), Nonmetric Multidimensional Scaling (NMS), and Isometric Feature Mapping (Isomap). The data set was obtained from the water quality monitoring networks operated by the Ministry of Agriculture and Forestry and the Ministry of Environments. Chlorophyll-${\alpha}$ displayed the highest correlation with COD, followed by TP, BOD, SS, and TN (p<0.01), while negatively correlated with altitude and bank height of the reservoirs (p<0.01). Although four different ordination methods similarly patterned the reservoirs according to the gradient of nutrient concentration, PCA and NMS appeared to be the most efficient methods to pattern water quality of reservoirs based on the explanation power. Considering variable scores in the ordination map, the concentration of nutrients was positively correlated with Chl-${\alpha}$, while negatively correlated with altitude and bank height. These ordination methods may help to pattern agricultural reservoirs according to their water quality characteristics.

• 한국환경과학회지
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• 제28권7호
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• pp.607-615
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• 2019

The purpose of this study is to biological treatment of high salinity wastewater using Aerobic Granular Sludge (AGS). In laboratory scale's experiments research was performed using a sequencing batch reactor, and evaluation of the denitrification reaction in accordance with the injection condition of salinity concentration, surface properties of microorganisms, and sludge precipitability was performed. The results showed that the salinity concentration increased up to 1.5%, and there was no significant difference in the nitrogen removal efficiency; however, it showed a tendency to decrease gradually from 2.0% onward. The specific denitrification rate (SDNR) was 0.052 - 0.134 mg $NO_3{^-}-N/mg$ MLVSS (mixed liquor volatile suspended solid)${\cdot}day$. The MLVSS/MLSS (mixed liquor suspended solid) ratio decreased to 76.2%, and sludge volume index ($SVI_{30}$) was finally lowered to 57 mL/g. Using an optical microscope, it was also observed that the initial size of the sludge was 0.2 mm, and finally it was formed to 0.8-1.0 mm. Therefore, salinity injection provides favorable conditions for the formation of an AGS, and it was possible to maintain stable granular sludge during long-term operation of the biological treatment system.

• The Journal of Asian Finance, Economics and Business
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• 제7권4호
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• pp.133-143
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• 2020

The paper investigates the factors affecting the profitability of commercial banks in Asian developing countries, including Vietnam, Malaysia and Thailand. We use panel data of four entities; ten banks in Vietnam, eight banks in Malaysia, nine banks in Thailand and all 27 commercial banks from the period 2012 to 2016. Particularly, Return on Asset, Return on Equity and TOBINQ are defined as profitability indicators, which are impacted by three main types of independent variables, namely bank-specifics, which include CAR, NPL, Cost to income, Liquidity ratio and Bank size, industry-specific variable-concentration HHI and macroeconomic-specific variables, which consist of GDP growth and Inflation. Using panel data regressions, the paper identifies several similarities and differences among empirical results on the models of four entities, each of three countries and the overall sample. The most outstanding similarity is that all entities record the significantly negative relationship between operational risk and banking profitability. Likewise, the significantly negative influence of bank size to profitability is found on models of Vietnam and Thailand and no significant effect on the model of Malaysia. Meanwhile, the most controversial result comes up with the negative relationship between CAR and profitability indicators as well as the positive association between credit risk and banking profitability.