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Neuroprotective Effects of Cambodian Plant Extracts on Glutamate-induced Cytotoxicity in HT22 Cells  

Keo, Samell (Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University)
Lee, Dong-Sung (Hanbang Body-Fluid Research Center, Wonkwang University)
Li, Bin (Hanbang Body-Fluid Research Center, Wonkwang University)
Choi, Hyun-Gyu (Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University)
Kim, Kyoung-Su (Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University)
Ko, Won-Min (Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University)
Oh, Hyun-Cheol (College of Pharmacy, Wonkwang University)
Kim, Youn-Chul (Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University)
Publication Information
Natural Product Sciences / v.18, no.3, 2012 , pp. 177-182 More about this Journal
Abstract
Oxidative stress potentially induces neurotoxicity which is believed to underlie several major age-related diseases of the central nervous system. This study sought to identify the cytoprotective effects of sixty-nine Cambodian plants against glutamate-induced cell death. Cultured HT22 cells were applied as an in vitro model, and neurotoxicity was induced in these neuronal cells by exposure to a determined concentration of glutamate. Sixty-nine plant sources, as Cambodia's indigenous species, were purchased from O'reusey Market, Phnom Penh, and extracted with ethanol. These extracts were screened for cytoprotective effects against glutamate-triggered neurotoxicity in HT22 cells at concentrations of 100 and 300 ${\mu}g/ml$. Of these, eight ethanol extracts, bark of Anacardium occidentale, bark and sapwood of Bauhinia pulla, flowers of Borassus flabellifer, stems and leaves of Coix lacryma-jobi, bark and sapwood of Diospyros nitida, sapwood of Dipterocarpus obtusifolius, stems of Oryza rufipogon, and fruits of Phyllanthus emblica, showed significant cytoprotective effects against glutamate-induced cell damage and degeneration in HT22 cells.
Keywords
HT22 cells; Glutamate; Cambodian plants; Neurotoxicity; Cytoprotective effect;
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