• Journal of the korean academy of Pediatric Dentistry
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• v.34 no.1
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• pp.36-42
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• 2007

To compare the survival rate of periodontal ligament cells preserved in storage media with good availability at the time of an avulsion injury, periodontal ligament cells were incubated in ${\alpha}-MEM$ culture medium containing 10% FBS in condition of $37^{\circ}C$, 5% $CO_2$. These cells were then cultured in HBSS, ${\alpha}-MEM$, milk(S co., P. co.) and tap water at the temperature of 4, 25, $37^{\circ}C$ each in 60 min. The groups were measured by MTT assay. The results were as follows : 1. Among the storage media at $4^{\circ}C$, ${\alpha}-MEM$ and P-milk had the highest preserving ability of periodontal ligament cells, while that of HBSS S-milk and tap was low in order. 2. Among the storage media at $25^{\circ}C$, ${\alpha}-MEM$ had the highest preserving ability of periodontal ligament cells, while that of P-milk, HBSS, S-milk, tap water was low in order. 3. Among the storage media at $37^{\circ}C$, the preserving ability of periodontal ligament cells was very high in ${\alpha}-MEM$, P-milk, HBSS and S-milk, it's lowest in tap water. 4. The preserving ability of periodontal ligament cells in ${\alpha}-MEM$ was high at $4^{\circ}C$ and it's low in order of $25^{\circ}C$, $37^{\circ}C$, but in HBSS was high at $4^{\circ}C$ and it's low at $25^{\circ}C$, $37^{\circ}C$ 5. The preserving ability of periodontal ligament cells in S-milk and P-milk was high at $4^{\circ}C$, $25^{\circ}C$ and it s low at $37^{\circ}C$. In conclusion, HBSS is the storage medium of choice in an avulsion, but in this study it is preferable to choose milk at $4^{\circ}C$ for tooth since it is easy to get and affect cell viability.

• Journal of Life Science
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• v.18 no.12
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• pp.1625-1630
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• 2008

A microorganism capable of producing high level of poly-3-hydoxybutyrate (PHB) from xylose was isolated from soil. The isolated strain J-65 was identified as Bacillus megaterium based on the morphological, biochemical and molecular biological characteristics. The optimum temperature and pH for the growth of B. megaterium J-65 were $37^{\circ}C$ and 8.0, respectively. The optimum medium composition for the cell growth was 2% xylose, 0.25% $(NH_4)_2SO_4$, 0.3% $Na_2HPO_4{\cdot}12H_2O$, and 0.1% $KH_2PO_4$. The optimum condition for PHB accumulation was same to the optimum condition for cell growth. Copolymer of ${\beta}$-hydroxybutyric and ${\beta}$-hydroxyvaleric acid was produced when propionic acid was added to shake flasks containing 20 g/l of xylose. Fermenter culture was carried out to produce the high concentration of PHB. In batch culture, cell mass was 9.82 g/l and PHB content was 35% of dry cell weight. PHB produced by B. megaterium J-65 was identified as homopolymer of 3-hydoxybutyric acid by GC and NMR.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.6
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• pp.774-780
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• 2000

For this study three types of block such as medicated urea-molasses-mineral block (MUMMB, 7% urea), urea-molasses-mineral blocks ($UMMB_a$ 7% urea) and urea-molasses-mineral blocks ($UMMB_b$ 21% urea) were prepared. Naturally infested 24 indigenous dairy cows with gastrointestinal nematodes were used to study the effect of MUMMB, $UMMB_a$ and $UMMB_b$ on different parameters (body weight, milk yield, serum urea level, milk urea level). The cows were offered normal diet added with 250 g block per cow for every day. Straw and green grasses were used as basal diet in the ration. The managemental facilities for all cows of each group were similar. Cows were stall fed, calves were tied up at night and had free access to their dam during day time. Milking was done once daily in the morning. The daily average live weight gains by MUMMB, $UMMB_a$, $UMMB_b$, and control group (no block) were 52.08 g, 44.44 g, 48.58 g, and 1.41 g respectively. The live weight gain were statistically significant (p<0.01) in comparison to control group. The highest daily milk yield was observed in MUMMB treated cows (2.39 L/d); followed by $UMMB_b$ treated cows (1.82 L/d) and $UMMB_a$ treated cows (2.16 L/d). The lowest milk yield was found in control group (1.54 L/d). This result differs significantly (p<0.05) between treated groups and also with control group. During urea-molasses-mineral blocks $UMMB_a$, $UMMB_b$ licking milk urea level increased from initial $22.76{\pm}2.35mg/dl$ to $35.46{\pm}4.80mg/dl$ and initial $22.86{\pm}2.96mg/dl$ to $40.66{\pm}0.87mg/dl$ respectively. This variation of milk urea level was statistically significant (p<0.001). Similarly during $UMMB_a$, and $UMMB_b$ blocks licking, serum urea level increased in both treated groups from initial $22.76{\pm}2.60mg/dl$ to $32.68{\pm}2.21$ and initial $23.70{\pm}2.78mg/dl$ to $40.48{\pm}3.24mg/dl$, respectively. This variation of serum urea level was also statistically significant (p<0.001). Use of MUMMB instead of UMMB was proved better for milk production and live weight gain in dairy cows under the village condition of Bangladesh where balanced ration for dairy cows are a major scarcity. And various concentration of urea in blocks positively affect milk and serum urea levels of indigenous dairy cows.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.13 no.1
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• pp.153-162
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• 2013

This paper proposes the most simple method for optimal solution of the transshipment problem. Usually the transshipment problem is solved by direct linear programming or TSM (Transportation Simplex Method). The method using TSM has two steps. First it is to get a initial solution using NCM, LCM, or VAM, second to refine the initial solution using MOD or SSM. However the steps is complex and difficult. The proposed method applies the method that transforms transshipment problem to transportation problem. In the proposed method it simply selects the minimum cost of rows about transportation problem, and then it applies the method that assigns a transported volume as an ascending sort of the costs of rows about the selected costs. Our method makes to be very fast got the initial value. Also we uses the method that controls assignment volume, if a heavy item of cost is assigned to a transported volume and it has a condition to be able to transform to more lower cost. The proposed algorithm simply got the optimal solution with applying to 11 transshipment problem.

• Korean Journal of Soil Science and Fertilizer
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• v.6 no.4
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• pp.213-219
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• 1973

Effects of three slow-release nitrogen fertilizers (Sulfur-coated urea, (SCU) Jin Hae Chemical Co. Korea(JHC); Tennessee Valley Authority. U.S.A(TVA); METAP, Japan) on the yield and nitrogen uptake of IR667 (Suwon 213) were investigated comparing with urea split application under the field condition with early and usual saeson cultivation. 1. SCU(JHC) was not superior than urea split application in yield and nitrogen nutrition due to early release of nitrogen. 2. SCU(TVA) outyielded urea split by 10% showed always highest nitrogen content in straw and most balanced relative cumulative curve of nitrogen uptake or dry matter yield. 3. At ear formation stage SCU(TVA) showed specially high nitrogen content which was highly correlated with number of grain per $m^2$ of field. 4. Only SCU(TVA) showed one peak curve of nitrogen uptake rate at ear formation stage while others showed two peak pattern. 5. nitrogen fertilizer recovery of slow-release fertilizers was higher than urea split in early season cultivation and SCU (TVA) (46.0) was higher but SCU(JHC) (38.9) was similar in compare with urea split(37.1) in usual season cultivation. 6. Most promising slow-release nitrogen to match plant requirement seems to be one mixed of various slow-release fertilizers having different release pattern.

• Korean Journal of Applied Biomechanics
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• v.12 no.2
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• pp.361-380
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• 2002

The purposes of this study were to a analysis of friction relation between tennis outsole and tennis playing surfaces. Tennis footwear is an important component of tennis game equipment. It can support or damage players performance and comfort. Most importantly athletic shoes protect the foot preventing abrasions and injuries. Footwear stability in court sports like tennis is incredibly important since it is estimated that as many as 45% of all lower extremity injuries occur in the foot and ankle. The friction force is the force exerted by a surface as an object moves across it or makes an effort to move across it. The friction force opposes the motion of the object. Friction results when two surfaces are pressed together closely, causing attractive intermolecular forces between the molecules of the two different surfaces. The outsole provides traction and reduces wear on the midsole. Today's outsoles address sport specific movements (running versus pivoting) and playing surface types. Different areas of the outsole are designed for the distinct frictional needs of specific movements. Traction created by the friction between the outsole and the surface allows the shoe to grip the surface. As surfaces, conditions and player motion change, traction may need to vary. An athletic shoe needs to grip well when running but not when pivoting. Laboratory tests have demonstrated force reductions compared to impact on concrete. There is a correlation between pain, injury and surface hardness. These are a variety of traction patterns on the soles of athletic shoes. Traction like any other shoe characteristic must be commensurate and balanced with the sport. The equal and opposite force does not necessarily travel back up your leg. The surface itself absorbs a portion of the force converting it to other forms of energy. Subsequently, tennis court surfaces are rated not only for pace but also for the percentage of force reduction.

• Magazine of the Korean Society of Agricultural Engineers
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• v.8 no.2
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• pp.1193-1199
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• 1966

More stable and higher yields in rice paddy depend mainly upon an adequately balanced supply of water for higher yield. Rice paddy is supplied naturally by rainfall but inevitably requires artificial supplenental irrigation for higher yields. Even though it may be true that the water requirement of rice plants is generally higher than those of other crops, the submerged condition is not necessarily required for rice. The moisture requirements of rice vary according to its growing stages and it is possible to increse the irrigation efficiency by means of lessening the loss due to percolation and evapolation in the field. This experiment was conducted on the effect of the various amount of water supply and different irrigation periods for yield and yield components, and was carried out to find out the most suitable irrigation method and to increase the irrigation efficiency for higher yields in rice paddy. Randomized block design with 3 replications was employed where the 3 levels of the amount of irrigation water; (120% moisture contents), unirrigated (90~100%) and more un irrigated candition (80~90% moisture content), and levels of the various irrigation periods; usual, initial, intermediate and final periods, being treated. The results obtained in this experiment are as follows: 1. As for the physical and chemical and soil properfies, and other characteristics, there are no differences among the treatments enough to be effective for the growth of rice plants. 2. Culm length was measured after harvest as shown in table 2. 3. Difference of the amount of irrigation water did not change the culm length and ear length, however it also indicated more apparent increase in final treatment plots thatn that of usual. 3. No difference in the number of ears and number of ears pers per hill was founded treatments both in the difference of water supply and in the various irrigation periods. 4. There is no difference in the maturing rate and 1000 grains weight. 5. The number of panicles and grains and more increased in 80~100% moisture contents soil than those of 120%. and it shows in un irrigated plots, more irrigated plots and control plots in turn. Other wise according to the period\ulcorner of irrigation the trend is appeared in turn initial, usual, final and intermediate treatments.6. Yield as shown in table 7. 8 was more increased in unirrigated plots(90~IOO% moisture content) than the control plots (120% moisture content) by up to 8.2% and also 3. 2% in more unirrigated plots than that of control by periods is shown: usual plots final, initial, inter mediate treatment plots in turn. 7. The above resutts lead to the conclusion that no remarkable, differences in yields and soil properties are made by the unirrigation. However, it is apparent that this treatment has .some advantages in the points that one could spare the amount of water supply for irrigation with more increase in yield. In addition, a higher temperature and a brisk oxygen supply would be possible throug h this treatments. Accordingly, these treatment would be a more reasonable and economical cultivation method of rice for the better harvest.

• Clinical and Experimental Reproductive Medicine
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• v.30 no.2
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• pp.111-118
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• 2003

Objectives: The development of an useful method for obtaining metaphase chromosomes from a biopsied blastomere would allow differentiation between embryos with balanced and normal chromosome complements in the preimplantation genetic diagnosis for chromosomal translocations. This study was performed to evaluate the effects of microtubule depolymerizing agents (MTDAs) on the blastomeres of mouse and human preimplantation embryos, and to establish an effective method for obtaining metaphase chromosomes of biopsied blastomeres in human early embryos. Materials and Methods: Early embryos (2-4 cell stage) from superovulated mice (ICR strain) were collected and treated with single or mixture MTDAs, such as vinblastine, nocodazole and colcemid. After the treatment of MTDAs for 16 hours, the metaphase aquisition (MA) rates were evaluated by the observation of chromosome status with bis-benzimide or DAPI staining. The optimal condition from the above experiment was applied to human embryos, which were developed from abnormal fertilization (3-pronuclei). Fluorescence in-situ hybridization (FISH) with whole chromosome probes was conducted on the human metaphase chromosomes by the MTDAs. Results: In mouse embryos, the effective concentrations of each MTDAs for obtaining metaphase chromosomes were $1.0{\mu}M$ of vinblastine (20.3%), $5.0{\mu}M$ of nocodazole (28.1%) and $1.0{\mu}M$ colcemid (55.6%), respectively. The highest MA rate (91.2%) in the mouse embryos was obtained by a mixture of vinblastine ($1.0{\mu}M$) and nocodazole ($1.0{\mu}M$). In the human embryos, the metaphase chromosomes of blastomeres were obtained in 44 of 113 blastomeres (38.9%) by treatment of the mixture of vinblastine and nocodazole. FISH signals of the metaphase chromosomes were successfully observed in human individual blastomeres. Conclusions: The treatment of a mixture MTDAs for obtaining metaphase chromosomes was an efficient method, and the MA rate was above 90% in the mouse embryos. However, only a relatively small proportions of the blastomeres yielded metaphase chromosomes by the MTDAs in the human embryos. The inconsistent effects of MTDAs may be related to the variation of different species and the poor developmental potency of abnormally fertilized human embryos. We should develop more reliable and efficient methods for obtaining the metaphase chromosomes in the biopsied blastomeres of human preimplantation embryos.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.2
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• pp.204-213
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• 2008

Four ruminally cannulated Holstein cows averaging 43 days in milk (DIM) were used in a $4{\times}4$ Latin square to determine the effect of feeding extruded soybean, ground canola seed and whole cottonseed on ruminal fermentation and milk fatty acid profile. One hundred and twenty lactating Holstein cows, 58 (${\pm}31$) DIM, were assigned to four treatments in a completely randomized block design to study the effects of the three types of oilseeds on production parameters and milk fatty acid profile. The four diets were a control diet (CON) and three diets in which 10% extruded soybean (ESB), 5% ground canola seed (GCS) and 10% whole cottonseed (WCS) were included, respectively. Diets consisted of concentrate mix, corn silage and Chinese wild rye and were balanced to similar concentrations of CP, NDF and ADF. Ruminal fermentation results showed that ruminal fermentation parameters, dry matter intake and milk yield were not significantly affected by treatments. However, compared with the control, feeding cows with the three oilseed diets reduced C14:0 and C16:0 and elevated C18:0 and C18:1 concentrations in milk, and feeding ESB increased C18:2 and cis9, trans11 conjugated linoleic acid (CLA). Production results showed that feeding ESB tended to increase actual milk yield (30.85 kg/d vs. 29.29 kg/d) and significantly decreased milk fat percentage (3.53% vs. 4.06%) compared with CON. Milk protein (3.41%) and solid non-fat (13.27%) from cows fed WCS were significantly higher than from cows fed CON (3.24% and 12.63%, respectively). Milk urea N concentrations from cows fed the ESB (164.12 mg/L) and GCS (169.91 mg/L) were higher than cows fed CON (132.31 mg/L). However, intake of DM, 4% fat corrected milk, energy corrected milk, milk fat and protein yields, milk lactose percentage and yield, somatic cell count and body condition score were not affected by different treatments. The proportion of medium-chain fatty acid with 14 to 16 C units in milk was greatly decreased in cows fed ESB, GCS and WCS. Feeding ESB increased the concentration in milk of C18:1, C18:2, C18:3 and cis9, trans11-CLA content by 16.67%, 37.36%, 95.24%, 72.22%, respectively, feeding GCS improved C18:0 and C18:1 by 17.41% and 33.28%, respectively, and feeding WCS increased C18:0 by 31.01% compared with feeding CON. Both ruminal fermentation and production trial results indicated that supplementation of extruded soybean, ground canola seed and whole cottonseed could elevate the desirable poly- and monounsaturated fatty acid and decrease the medium chain fatty acid and saturated fatty acid content of milk fat without negative effects on ruminal fermentation and lactation performance.

• The KIPS Transactions:PartC
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• v.12C no.1 s.97
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• pp.121-128
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• 2005

Tho existing TCP(Transmission Control Protocol) is known to be unsuitable for a network with the characteristics of high RDP(Bandwidth-Delay Product) because of the fixed small or large buffer size at the TCP sender and receiver. Thus, some trial cases of adjusting the buffer sizes automatically with respect to network condition have been proposed to improve the end-to-end TCP throughput. ATBT(Automatic TCP fluffer Tuning) attempts to assure the buffer size of TCP sender according to its current congestion window size but the ATBT assumes that the buffer size of TCP receiver is maximum value that operating system defines. In DRS(Dynamic Right Sizing), by estimating the TCP arrival data of two times the amount TCP data received previously, the TCP receiver simply reserves the buffer size for the next arrival, accordingly. However, we do not need to reserve exactly two times of buffer size because of the possibility of TCP segment loss. We propose an efficient TCP buffer tuning technique(called TBT-PLR: TCP buffer tuning algorithm based on packet loss ratio) since we adopt the ATBT mechanism and the TBT-PLR mechanism for the TCP sender and the TCP receiver, respectively. For the purpose of testing the actual TCP performance, we implemented our TBT-PLR by modifying the linux kernel version 2.4.18 and evaluated the TCP performance by comparing TBT-PLR with the TCP schemes of the fixed buffer size. As a result, more balanced usage among TCP connections was obtained.