• Title/Summary/Keyword: Bacterium

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단백질분해효소를 생성분비하는 호알칼리성 Coryneform bacterium TU-19의 분리 및 동정

  • 최명철;양재섭;강선철
    • Microbiology and Biotechnology Letters
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    • v.24 no.2
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    • pp.160-165
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    • 1996
  • An alkalophilic bacterium producing alkaline protease(s) was isolated from soil. It was a Gram-positive, non-sporulating, immotile, irregular rod, strictly aerobic, and weak acid-forming bacterium. The morphological, physiological, and biochemical characteristics of the isolate resembled those of the Coryneform bacteria. However, there was not any species within this genera to which this microorganism can be closely matched. Therefore, it is provisionally identified as a Coryneform bacterium TU-19.

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Producyion of Threonine Using Methanol Dehydrogenase and Serine Hydroxyltransferase in a New Methylotrophic Bacterium KJ29 (New Methylotrophic Bacterium KJ29의 Methanol Dehydrogenase와 Serine Hydroxymethyltransferase를 이용한 Threonine의 생산에 관한 연구)

  • 김경자
    • Microbiology and Biotechnology Letters
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    • v.21 no.6
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    • pp.577-581
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    • 1993
  • The amino acid threonine was produced from glycine and ethanol in a reaction mixture using cell free extract of the methylotrophic bacterium isolated from soil and identified as mellthylo-bacterium sp. KJ29. Although the isolate could grow on carbon source other than methanol, only the cell free extract from the cells grown on methanol produced threonine. Methanol dehydrogenase (MDH) activity was present only in the cells grown on methanol when compared to the cells grown on heterotrophic substrates.

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The Bacterial Gall of Wistaria floribunda Caused by Pantoea agglomerans pv, milletiae (Pantoea agglomerans pv. milletiae에 의한 등나무 혹병)

  • 김종완;임진우
    • Research in Plant Disease
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    • v.7 no.3
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    • pp.145-149
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    • 2001
  • A pathogenic bacterium was isolated from galls on Wistaria floibunda at Jinryang of Kyungsan, Kyungbuk, Korea in May, 2000. This bacterium was cultured and tested for pathogenicity on the host. Also its morphological and physiological characteristics were examined. Inoculation with the bacterium isolated from the gall caused the same symptoms as those under natural conditions. On the basis of bacteriological characteristics and pathogenecity on the host plant of the organism, the causal bacterium was identified as Pantoea agglomerans pv. milletiae. This is the first report of this bacterium to occur on W. floribunda plant in Korea. Therefore we proposed to name the disease as "bacterial gall of Wistaria floribunda" by P. agglomerans pv. milletiae.milletiae.

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Isolation of the Agarolytic Bacterium Vibrio cyclotrophicus DAG-130 from Abalone Gut

  • Meinita, Maria Dyah Nur;Luyen, Hai-Quoc;Hwang, Seon-Yeong;Kang, Ji-Young;Jin, Deuk-Hee;Hong, Yong-Ki
    • Fisheries and Aquatic Sciences
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    • v.11 no.2
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    • pp.76-81
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    • 2008
  • We isolated 1,916 strains of bacteria from gut and feces of abalone. The most active agarolytic bacterium, DAG-130, was identified from the gut of the abalone Haliotis gigantea. Of the bacteria harbored by both H. discus hannai and H. gigantea, 59% were agarolytic. There was no significant difference in the number of agarolytic bacteria isolated from abalone fed on the seaweeds Gelidium amansii, Laminaria japonica, or Undaria pinnatifida. Of the agarolytic bacteria, 72% were isolated from the guts of all sources tested while 43% came from the feces. The strain DAG-130 showed 100% identity with the bacterium Vibrio cyclotrophicus based on phylogenetic analysis of l6S rDNA. The bacterium produced monomers and oligomers from the agar substrate.

Effects of Predator Addition to the Algicidal Bacterium in Controlling Diatom Sephanodiscus hantzschii Dominating the Eutrophic Pal′tang Reservoir, Korea (살조세균과 초식성 섭식자의 혼합배양에 의한 녹조제어효과)

  • Kim Baik-Ho;Ka Soon-Kyu;Han Myung-Soo
    • Journal of environmental and Sanitary engineering
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    • v.19 no.2
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    • pp.23-29
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    • 2004
  • An algicidal effect of endemic algicidal bacterium (Pseudomonas putida) and rotifer zooplankton (Brachionus calyciflorus) on diatom Stephanodiscus hantzschii were examined in the filtered water, and were compared with those of bacterium plus ciliate. Bacteria removed as 80% of the diatom within 3.5 days, while ciliate and zooplankton suppressed 57% and 40% of diatom during the same period, respectively. Mixed treatment of bacteria plus ciliate removed as 54% of diatoms, while that of bacteria plus zooplankton decreased as 85%. Although single bacteria and mixed treatment of bacteria plus zooplankton quickly decreased the diatom in the initial of experiment, bacteria plus ciliate perfectly removed the diatom in culture flask within 5.5 days of the study. On the other hand, other single and mixed treatments did not clear the diatom during the same period, and over 10% of them still remain in culture flask. Predator biomass in the presence of algicidal bacteria showed the growth patterns; zooplankton gradually decreased, and ciliate sustained over 0.5 cells/ml. These results indicated that the addition of ciliate to the algicidal bacterium in controlling the diatom Stephanodiscus hantzschii is more effective than that of zooplankton. Therefore, this synergistic interaction relationship between the bacterium and ciliate play an important role in the bio-manipulation using bio-agents to control the diatomal bloom in freshwater lakes and streams.

Characteristics of Algicide Produced by Micrococcus luteus SY-13 Inhibiting Cochlodinium polykrikoides and the Effects on Marine Organisms (적조생물 Cochlodinium polykrikoides를 저해하는 Micrococcus luteus SY-13이 생산하는 살조물질의 특성과 해양생물에 미치는 영향)

  • Kim, Min-Ju;Jeong, Seong-Yun;Cha, Mi-Sun;Lee, Sang-Joon
    • Journal of Environmental Science International
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    • v.17 no.4
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    • pp.439-449
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    • 2008
  • Algicidal bacterium was isolated from sea water during the declining period of Cochlodinium polykrikoides blooms and this bacterium had a significant algicidal activity against C. polykrikoides. In this study, algicidal bacterium was identified on the basis of biochemical and chemotaxonomic characteristics, and analysis of 16S rDNA sequences. The algicidal bacterium showed 98.6% homology with Micrococcus luteus ATCC $381^T$. Therefore, this bacterium was designated Micrococcus luteus SY-13. The optimal culture conditions of the algicidal bacterium was $25^{\circ}C$, initial pH 8.0, and 3.0% NaCl concentration. M. luteus SY-13 is assumed to produce secondary metabolites which have algicidal activity. When 10% culture filtrate of this strain was applied to C. polykrikoides ($1.0\;{\times}\;10^4\;cells/ml$) cultures, over 98% of C, polykrikoides cells were destroyed within 6 hours. The culture filtrate of M. luteus SY-13 exhibited similar algicidal activity after heat-treatment at $121^{\circ}C$ for 15 min. While algicidal activity remained in filtrates with pH adjusted to 8.0, loss of algicidal activity occurred when the pHs of filtrates were adjusted to over 9.0 or heat-treated at $121{\times}180^{\circ}C$ for 1 hour. M. luteus SY-13 showed significant algicidal activities against C. polykrikoides (98.9%) and a wide algicidal range against various harmful algal bloom (HAB) species. However, there was no algicidal effect on diatom and marine livefood organisms except Isocrysis galbana. These results suggest that M. luteus SY-13 could be a candidate for use in the control of HABs.

Isolation and identification of a phenol-degrading bacterium from the sewage sludge (하수슬러지로부터 페놀분해세균의 분리 및 동정에 관한 연구)

  • Kim, Young-Jun;Lee, Suk-Won;Han, Gee-Bong
    • Journal of the Korea Organic Resources Recycling Association
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    • v.12 no.1
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    • pp.67-74
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    • 2004
  • A bacterium which grow on phenol as an only carbon and energy source was isolated from the sewage sludge at Nangi municipal wastewater treatment plant in Seoul. This bacterium was found to be a Gram negative rod with high motility, and well grew on 0.05%, 0.1%, and 0.15% of phenol. No matching strain was found from the result of the BBL test. Phylogenetic analysis of the strain by comparison of the 16s-rDNA has revealed that this bacterium has 99% of similarity with Stenotrophomonas maltophilia strain of Xanthomonas group, which belongs t the Gamma (${\gamma}$) subdivision of Proteobacteria. This strain has also shown 98% of similarity with nitrogen fixing bacterium MAGDE3 and Pseudomonas cissicola strain, and 97% of similarity with Stenotrophomonas sp. LMG198 and Xanthomonas cucurbitae.

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Isolation of a Medium Chain Length Polyhydroxyalkanoic Acids Degrading Bacterium, Janthinobacterium lividum

  • Park, Jin-Seo;Park, Jeong-Youl;Joung, Pil-Mun;Park, Seong-Joo;Rhee, Young-Ha;Shin, Kwang-Soo
    • Journal of Microbiology
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    • v.39 no.2
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    • pp.139-141
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    • 2001
  • Medium-chain length polyhydrexyalkanoic acids (MCL-PHAs) degrading bacterium was isolated from the soil. The bacterium was identified as Janthinobacterium lividum by its biochemical properties, cell membrane fatty acids composition, and 16S rDNA sequence analysis. The bacterium showed a similarity of 0.911 with J. lividum according to the cell membrane fatty acids analysis and a similarity of 97% in the 16S rDNA requence analysis. Culture supernatant of the bacterium skewed the highest depolymerase activity toward polyhydroxynonanoic acid (PHN) that did not degrade the poly-$\beta$-hydroxybutyric acid (PHB). The esterase activity was also detected with p-nitrophenyl (PNP) esters of fatty acids such as PNP-dodecanoic PNP-dodecanoic acid, PNP-decanoic acid, and PNP-hexanoic acid.

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Polymorphism Of A Deep Marine Benthic Bacterium From The Gulf Of Mexico

  • Blanton, W.George;Blanton, Carol J.
    • 한국해양학회지
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    • v.3 no.1
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    • pp.8-15
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    • 1968
  • An interesting bacterium (#271) was isolated from the abyssal plain of the Sigsbee Deep of the Gulf of Mexico. The organism exhibits marked polymorphism (baciloid, coryneform and myceloid morphologies) in response to certain cultural conditions. the organism has been observed undergoing reproduction by transverse fission, fragmentation and arthrospore production. The presence of arthrospores indicates the bacterium is a member of the genus Arthrobacter; however, computed affinity coefficients do not confirm this genus. Until further studies have been completed on this isolate the authors are reluctant to place it in a generic group.

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Isolation and Identification of a Photosynthetic Bacterium Containing $Q_{10}$ ($Q_{10}$ 함유 광합성세균의 분리 및 동정)

  • Jeong, Soo Kyoung;Kim, Joong Kyun
    • Journal of Marine Bioscience and Biotechnology
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    • v.2 no.2
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    • pp.120-122
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    • 2007
  • A $Q_{10}$-producing photosynthetic bacterium was isolated from the silt at Nakdong river. The isolate had 1.55 mg of $Q_{10}$ per gram of dry cell. By the 16s-rDNA sequence analysis, the isolate was found to be Rhodobacter sphaeroids with 100% similarity (Genbank Accession No.=AM696701).

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