• Annals of Clinical Microbiology
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• 제21권4호
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• pp.75-79
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• 2018

배경: 요배양검사는 요로감염 진단을 위한 표준검사로 가장 흔히 의뢰되는 미생물 배양 검사 중 하나이다. 소변 자동분석기는 감염과 관련된 많은 정보를 제공한다. 최근 개발된 Sysmex UF-5000 (Sysmex, Japan)은 유세포분석 방법에 의해 세균, 효모균, 백혈구 등의 입자를 정량적으로 측정하고, 그람 염색성 정보를 제공한다. 저자들은 UF-5000을 이용하여 불필요한 요배양검사를 얼마나 선별할 수 있는지 평가하였다. 방법: 요배양검사가 의뢰된 453 검체 중 비뇨기과/신장내과 의뢰 검체를 제외한 126 검체를 대상으로 요시험지봉검사와 UF-5000으로 검사를 시행하여 요배양검사 결과와 비교하였다. 소변 배양은 집락수가 $10^4CFU/mL$ 이상인 경우 양성으로 판정하였다. 결과: UF-5000의 세균 수 $50/{\mu}L$이하, 효모양 세포 $100/{\mu}L$ 이하를 기준으로 했을 때 분석 대상 요배양의 38.1% (48/126), 전체 요배양 453건의 10.6%를 불필요한 요배양검사로 선별해 낼 수 있었다. 결론: UF-5000에서 산출된 세균 및 효모양 세포의 수로 음성 배양 결과를 예측할 수 있으며 약 10%의 불필요한 배양검사를 줄일 수 있다.

• 식물병연구
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• 제18권3호
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• pp.210-216
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• 2012

Plasmodiophora brassicae에 의한 배추 뿌리혹병은 배추뿐만 아니라 순무, 양배추에 발병하여 큰 피해를 주는 병원균이다. 배추 뿌리혹병균에 길항효과가 있는 미생물을 선발하기 위하여 배추 조직에서 분리한 Flavobacterium hercynium EPB-C313 균주의 항균활성 검정을 하였고, 포장시험을 통하여 배추 뿌리혹병에 대한 방제효과를 조사하였다. F. hercynium EPB-C313의 균체, 배양액, 배양여액를 배추 뿌리혹병 휴면포자와 혼합 처리하여 24시간 후 조사하였을 때 각각 90.4%, 36.8% 및 26.0%의 휴면포자가 불활성화 되었다. 온실 포트검정에서는 배추의 어린묘를 F. hercynium EPB-C313 배양액에 침지한 후 정식하면 뿌리혹 형성 억제율이 100%로 대조약제 fluazinam액상수 화제의 91.7%보다 우수한 효과를 나타내었다. 배추 뿌리혹병이 50% 이상 발병되었던 재배포장에 F. hercynium EPB-C313 혼합펠렛을 토양혼화 하고, F. hercynium EPBC313 배양액의 유묘관주 및 정식 10일 후 1회 토양관주하면 63.7%의 방제효과를 보였다. 이상의 결과로 볼 때 F. hercynium EPB-C313 균주는 매우 유용한 배추 뿌리혹병 생물적 방제제로 판단된다.

• 대한미생물학회지
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• 제11권1호
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• pp.27-32
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• 1976

Sodium amylosulfate(SAS) has been reported to be an effective substance to inactivate the anti-bacterial activity of blood in blood culture media. The advantage of the use of SAS over sodium polyanethol sulfonate(SPS) is that it does not inhibit the growth of some bacteria! species which are known to be inhibited by SPS. As to S. typhi, SPS is reported to enhance the growth, however the effect of SAS on this organism is not known as yet. Using 43 strains of S. typhi, isolated from clinical materials, the authors tried to determine the effect of SAS on this organism. The methods used for this study were : the SPS and SAS paper disk I sensitivity test, tests on the growth in trypticase soy broth(TSB) with SPS and with SAS, and experimental blood culture in SPS and SAS incorporated TSB. The following results were obtined. 1). S. typhi strains with the turbidity of No. 0.5 tube of MacFarland nepherometer were inoculated onto Mueller-Hinton plate and 1mg disk of SPS and SAS were applied. After 24-hour incubation, none of the 43 strains showed inhibition zone by SPS disk, but all of them showed zones by SAS disk with a mean zone diameter of 9.5mm(Table 1). 2) Inocula consisting of one to 54 viable counts of 37 strains were inoculated into three different media; TSB with 0.05% SPS, TSB with 0.05% SAS and TSB alone. After 24-hour incubation the mean of the optical densities of each medium were 0.483, 0.482 and 0.459 respectively, showing that SAS does not inhibit the growth of S. typhi. Moreover it was shown that there was no correlation between the amount of inocula and growth(Table 2 and Fig. 1). 3). Each set of media in 5 ml amounts consisting of one tube of TSB with 0.05% SPS, one tube of TSB with 0.05% SAS and two tubes of TSB were inoculated with 8, 64. 640 and 6400 viable counts of bacteria. Then 0.5 ml of fresh normal blood was added to all tubes except for one tube of TSB. Macroscopic observation after 24 hour incubation showed a heavy growth in all tubes except for the tube of TSB plus blood, which showed only a light growth in the tube of the heaviest inoculum. This result clearly demonstrates that the growth of S. typhi is inhibited by some antibacterial activities of fresh blood, which are counter acted by SPS and SAS(Table 3). Between SPS and SAS, there was no significant difference found(Table 4 and Fig. 2). With all these results it can be postulated that the addition of SAS into a rountine blood culture media may raise the positivity of S. typhi isolation and shorten the incubation period.

• 한국응용곤충학회지
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• 제51권1호
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• pp.39-45
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• 2012

곤충병원세균인 제노랍두스($Xenorhabdus$ sp.)는 곤충병원선충인 $Steinernema$ $monticolum$으로부터 분리되었다. 이 세균 배양액을 배추좀나방($Plutella$ $xylostella$) 혈강에 주입할 경우 높은 병원력을 나타내지만, 섭식 처리할 경우 낮은 병원력을 보였다. 본 연구는 이 제노랍두스 세균 배양액이 $Bacillus$ $thuringiensis$(비티)와 혼합하여 배추좀나방 종령충에 처리할 경우 비티의 병원력을 뚜렷하게 증가시키는 것을 보였다. 또한 제노랍두스 배양액과 비티의 혼합비율을 달리할 경우 병원력이 크게 차이를 보였다. 최적의 두 세균 혼합비율을 이용하여 야외에 발생한 배추좀나방에 처리하였으며, 비티 단독처리에 비해 뚜렷이 상승된 방제 효과를 확인할 수 있었다. 이러한 결과는 제노랍두스 배양액을 비티와 혼합하여 새로운 미생물 살충제로 개발할 수 있는 가능성을 제시했다.

• 한국환경과학회지
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• 제6권2호
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• pp.173-182
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• 1997

The bacterial strains, which utilizes 2,4,4'-trichloro-2'-hydroxydiphenyl ether(TCHDPE) as a sole carbon source, were isolated by selective enrichment culture from soil samples of industrial waste deposits. The bacterium that showed the highestt biodegradation activity was designated as EL-O47R The isolated strain EL-O47R was Identified as the genus Pseudomonas from the results of morphological, cultural, and biochemical tests. The optimum conditions of medium for the growth and the degradation of TCHDPE were TCHDPE 500 ppm, (NH4)2SO4 0.1% as the nitrogen source, initial pH 7.0±0.1, and 37℃, respectively. In this conditions, the regradation rate of TCHDPE was about 97%. Pseudomonas sp. EL-O47R was tested for resistance to several metal compounds and antibiotics. Pseudomonas sp. EL-O47R was moderately grown to Cd(NO3)2, ZnCl2, AgSO4, CuSO4 and HgCl2. This strain was sensitive to rifampicin and kanamycln but resistant to ampicillin, penicillin, tetracyclin and chloramphenlcol. Pseudomonas sp. EL-O47R was grown structurally related com- pounds and potential metabolites of TCHDPE, and has the stability on TCHDPE biodegradation.

• 대한치과교정학회지
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• 제47권1호
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• pp.3-10
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• 2017

Objective: Microbial aggregation around dental implants can lead to loss/loosening of the implants. This study was aimed at surface treating titanium microimplants with silver nanoparticles (AgNPs) to achieve antibacterial properties. Methods: AgNP-modified titanium microimplants (Ti-nAg) were prepared using two methods. The first method involved coating the microimplants with regular AgNPs (Ti-AgNP) and the second involved coating them with a AgNP-coated biopolymer (Ti-BP-AgNP). The topologies, microstructures, and chemical compositions of the surfaces of the Ti-nAg were characterized by scanning electron microscopy (SEM) equipped with energy-dispersive spectrometer (EDS) and X-ray photoelectron spectroscopy (XPS). Disk diffusion tests using Streptococcus mutans, Streptococcus sanguinis, and Aggregatibacter actinomycetemcomitans were performed to test the antibacterial activity of the Ti-nAg microimplants. Results: SEM revealed that only a meager amount of AgNPs was sparsely deposited on the Ti-AgNP surface with the first method, while a layer of AgNP-coated biopolymer extended along the Ti-BP-AgNP surface in the second method. The diameters of the coated nanoparticles were in the range of 10 to 30 nm. EDS revealed 1.05 atomic % of Ag on the surface of the Ti-AgNP and an astounding 21.2 atomic % on the surface of the Ti-BP-AgNP. XPS confirmed the metallic state of silver on the Ti-BP-AgNP surface. After 24 hours of incubation, clear zones of inhibition were seen around the Ti-BP-AgNP microimplants in all three test bacterial culture plates, whereas no antibacterial effect was observed with the Ti-AgNP microimplants. Conclusions: Titanium microimplants modified with Ti-BP-AgNP exhibit excellent antibacterial properties, making them a promising implantable biomaterial.

• International Journal of Oral Biology
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• 제31권1호
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• pp.7-10
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• 2006

Mitis-salivarius sucrose bacitracin(MSB) medium is widely used in the selective isolation of mutans streptococci(MS), a designation for a group of oral cariogenic species. Recently, we have isolated three bacterial strains grown on MSB agar from human dental plaques. The three strains exhibited biochemical characteristics similar to those of the biotype IV of MS, with the exception that they manifested a positive reaction for arginine deaminase. The objective of this study was to identify and characterize these three clinical isolates. The bacteria were identified with biochemical tests as well as by 16S rDNA cloning and sequencing. In order to compare the antibiotics susceptibility of the clinical isolates with that of type strain, the minimum inhibitory concentrations of 9 antibiotics were determined using broth dilution assays. The results identified all of our three clinical isolates as Enterococcus faecalis. All E. faecalis strains were found to be susceptible to penicillin G, amoxicillin, augmentin, and vancomycin, but were resistant to ciprofloxacin, cefuroxim axetil, and clindamycin. Our findings indicate that E. faecalis is capable of growing on MSB agar, and suggest that the MSB medium be improved so that only MS should be recoverable on the medium, as originally devised for their selection.

• 한국임상수의학회지
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• 제26권4호
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• pp.336-339
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• 2009

5킬로그램의 4년령의 중성화된 수컷 시츄견이 식욕부진과 만성 기침을 주증상으로 내원하였다. 이 환견은 병력청취, 신체검사, 임상병리검사, 방사선 진단, 심초음파 검사 및 기관지경 검사를 근거로 기관기관지 이물로 진단되었다. 그 이물은 밥알 부스러기들로 확인되었고 기관지경을 이용한 세기관지 세척 후 진공 흡인을 통해 제거되었다. 진공 흡인으로 채취된 세척 이물의 세균 및 곰팡이 배양과 폐 기생충에 대한 검사는 음성을 보였다. 환견은 진단 후 7일 동안 테오필린, 프레드니솔론, 독시사이클린, 엔로플록사신, 아세틸시스테인, 실리마린, 수크랄페이트를 처방 받았다. 이물제거와 약물치료 후 식욕의 증진과 기침 증상은 완전히 없어졌다. 이 증례 보고는 기관지경 기술을 통해 호흡기의 이물의 진단평가와 제거가 가능함을 보여준다.

• 대한한방내과학회지
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• 제37권6호
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• pp.1042-1050
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• 2016

This case study reports on the effect of Korean medicine on a catheter-associated urinary tract infection (CAUTI) caused by multidrug-resistant Pseudomonas aeruginosa. An 83-year-old man diagnosed with stroke had dysuria, and it was found that an indwelling urinary catheter led to CAUTI. From laboratory tests, we identified multidrug-resistant Pseudomonas aeruginosa and applied Korean medicine to him. After herbal medication with acupuncture and moxibustion, we studied a urinalysis and urine culture again for follow-up. We found meaningful improvement in bacteriuria and bacterial identification. This case suggests that Korean medicine could have a beneficial effect on urinary tract infections caused by multidrug-resistant Pseudomonas aeruginosa.

• Asian-Australasian Journal of Animal Sciences
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• 제15권5호
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• pp.751-756
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• 2002

A study was carried out to evaluate the incidences and causes of bovine mastitis in Tanzanian shorthorn zebu (Bos indicus) in the traditional sector and crossbred cows (Bos taurus${\times}$Bos indicus) in the dairy ranching sector, both found under the extensive range management system. Management practices were evaluated through a survey study using structured questionnaires. A total of 120 lactating cows (60 cows from each sector) were screened for the disease using the California Mastitis Test (CMT). Confirmatory tests used for infected cows included; the Direct Microscopic Somatic Cell Count (DMSCC), culture, bacteriological and biochemical laboratory assays. Survey results showed that management practices were generally very poor in both sectors with 84% of the surveyed herds being kept and milked under very unhygienic environmental conditions. The level of infection was higher in the crossbred cows (5% clinical and 38.3% sub-clinical mastitis) and lower in the zebu cows with only sub-clinical mastitis (23.3%). Crossbred cows had (p<0.05) higher somatic cell counts than zebu cows. The four highest-ranking bacterial isolates in order of importance were Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae and Bacillus spp. It was concluded that bovine mastitis under the extensive management system in Tanzania was a result of poor management practices and that zebu cows were more resistant to the diseases than crossbred cows.