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http://dx.doi.org/10.5656/KSAE.2011.12.0.076

Enhanced Pathogenicity of Bacillus thuringiensis Mixed with a Culture Broth of an Entomopathogenic Bacterium, Xenorhabdus sp.  

Seo, Sam-Yeol (Department of Bioresource Sciences, Andong National University)
Ahn, Haet-Nim (Department of Bioresource Sciences, Andong National University)
Eom, Seong-Hyeon (Department of Bioresource Sciences, Andong National University)
Im, Eun-Yeong (Department of Bioresource Sciences, Andong National University)
Park, Ji-Young (Department of Bioresource Sciences, Andong National University)
Kim, Yong-Gyun (Department of Bioresource Sciences, Andong National University)
Publication Information
Korean journal of applied entomology / v.51, no.1, 2012 , pp. 39-45 More about this Journal
Abstract
The entomopathogenic bacterium, $Xenorhabdus$ sp., was isolated from an entomopathogenic nematode, $Steinernema$ $monticolum$. When these bacteria were injected into the hemocoel of the diamondback moth, $Plutella$ $xylostella$, they caused significant mortality. However, the bacterium was not pathogenic when it was administered orally. This study showed that $Xenorhabdus$ sp. significantly enhanced oral pathogenicity of $Bacillus$ $thuringiensis$ (Bt) against the last instar larvae of $P.$ $xylostella$. Different ratios of culture broth of $Xenorhabdus$ sp. and Bt showed significantly different pathogenicities against $P.$ $xylostella$. In field tests, the optimal bacterial mixture significantly enhanced control efficacy against $P.$ $xylostella$ compared to Bt treatment alone. These results demonstrated that $Xenorhabdus$ sp. culture broth can be developed as a potent biopesticide by enhancing the insecticidal efficacy of Bt.
Keywords
Entomopathogenic bacterium; Bacillus thuringiensis(Bt); Plutella xylostella; Biopesticide;
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