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http://dx.doi.org/10.5423/RPD.2012.18.3.210

Biocontrol Efficacy of Endophytic Bacteria Flavobacterium hercynim EPB-C313 for Control of Chinese Cabbage Clubroot  

Hahm, Soo-Sang (Chungnam Agricultural Research and Extension Services)
Kim, Jong-Tae (Chungnam Agricultural Research and Extension Services)
Han, Kwang-Seop (Chungnam Agricultural Research and Extension Services)
Kim, Byung-Ryun (Chungnam Agricultural Research and Extension Services)
Kim, Hong-Kyu (Chungnam Agricultural Research and Extension Services)
Nam, Yun-Kyu (Chungnam Agricultural Research and Extension Services)
Yu, Seung-Hun (Department of Applied Biology, Chungnam National University)
Publication Information
Research in Plant Disease / v.18, no.3, 2012 , pp. 210-216 More about this Journal
Abstract
Clubroot of Chinese cabbage by Plasmodiophora brassicae, was found to be high virulent to the Chinese cabbage, turnips and cabbage. It this study, the endophytic bacteria Flavobacterium hercynium EPB-C313, which was isolated from tissues of Chinese cabbage, was investigated the antimicrobial activity on the inactivation of resting spores and its control effect on clubroot disease by bioassay. The bacterial cells, culture solutions, and culture filtrates of F. hercynium EPB-C313 inactivate the resting spores of P. brassicae with 90.4, 36.8, and 26.0%, respectively. The clubroot was inhibited with 100% by dipping the seedlings of Chinese cabbage in culture solutions of F. hercynium EPB-C313 before planting, however the chemmical 'fluazinam' was 91.7% in pot tests. Complex treatment were highly enhanced control efficacy with 63.7% at field of 50% diseased plants by soil incorporation with the pellet contains organic matter and F. hercynium EPB-C313, seedling drench of culture solution of F. hercynium EPB-C313 and soil drench with 10 days after planting. These results imply that the F. hercynium EPB-C313 is a very useful biological control agent of clubroot disease of Chinese cabbage.
Keywords
Biological control; Chinese cabbage clubroot; Flavobacterium hercynium; Endophytic bacteria;
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