• Title/Summary/Keyword: Bacterial Biomass

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Properties of a Hexane-Degrading Consortium (Hexane 분해 혼합균의 특성)

  • Lee Eun-Hee;Kim Jaisoo;Cho Kyung-Suk
    • Microbiology and Biotechnology Letters
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    • v.33 no.3
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    • pp.215-221
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    • 2005
  • It was characterized the hexane biodegradation and mineralization using a hexane-degrading consortium, and analyzed its bacterial community structure by 16S rDNA PCR-DGGE (denaturing gradient gel electrophoresis). The specific growth rate (${\mu}_{max}$) of the hexane-degrading consortium was 0.2 $h^{-1}$ in mineral salt medium supplemented with hexane as a sole carbon source. The maximum degradation rate ($V_{max}$) and saturation constant ($K_{s}$) of hexane of the consortium are 460 ${\mu}mol{\cdot}g-DCW^{-1}{\cdot}h^{-1}$ and 25.87 mM, respectively. In addition, this consortium could mineralize $49.1{\%}$ of $^{14}C$-hexane to $^{14}CO_2$, and $43.6{\%}$ of $^{14}C$-hexane) was used for the growth of biomass. The clones isolated from the DGGE bands were closely related to the bacteria which were capable of degrading pollutants such as oil, biphenyl, PCE, and waste gases. The hexane-degrading consortium obtained in this study can be applied for the biological treatment of hexane.

Studies on Marine Heterotrophic Protists in Masan Bay, Korea (마산만에서 부유원생동물의 연구)

  • Lee, Won-Je;Shin, Kyung-Soon;Lee, Jae-Do
    • Ocean and Polar Research
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    • v.29 no.4
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    • pp.401-410
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    • 2007
  • In Korea the study of marine heterotrophic protists started in the late 1980s, and since the early 1990s many studies have been conducted in various marine environments. In this article, studies on the distribution and abundance of protists and the biotic interactions(bacteria-protists, phytoplankton-protists) conducted in Korean coastal waters are reviewed, and a field study is reported and discussed. The field study in Masan Bay was carried out from February 2004 to November 2005 at seven selected stations representative of the bay. During the study, the mean abundance of heterotrophic bacteria and the mean concentration of chlorophyll-a were $2.1{\times}10^6\;cells\;mL^{-1}$ and $9.8{\mu}g\;L^{-1}$, respectively. Heterotrophic protists consisted of heterotrophic dinoflagellates, heterotrophic nanoflagellates(excluding dinoflagellates) and ciliates, and their abundances were means of $7.9{\times}10^4\;cells\;L^{-1}$, $1.2[\times}10^3\;cells\;mL^{-1}$, and $4.0{\times}10^4\;cells\;L^{-1}$, respectively. Generally, the chlorophyll-a concentra+CZ14tions and the abundances of heterotrophic bacteria and protists were higher in the inner zone of the bay, where there are high concentrations of organic matters, than in the middle and outer zones. Using the grazing rates of heterotrophic nanoflagellates on bacteria previously reported in this area, it can be calculated that about 69% of bacterial producton was removed by HNF grazing activity. About 24% of initial chlorophyll-a concentration was removed by microzooplankton grazing activity. In conclusion, this study suggests that in Masan Bay heterotrophic protists control the growth of bacteria and phytoplankton, and heterotrophic protists represent an important link of bacterial & microalgal biomass to higher trophic levels.

The Role of Protozoa in Feed Digestion - Review -

  • Jouany, J.P.;Ushida, K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.12 no.1
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    • pp.113-128
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    • 1999
  • Protozoa can represent as half of the total rumen microbial biomass. Around 10 genera are generally present on the same time in the rumen. Based on nutritional aspects they can be divided in large entodiniomorphs, small entodiniomorphs and isotrichs. Their feeding behaviour and their enzymatic activities differ considerably. Many comparisons between defaunated and refaunated animals were carried out during the last two decades to explain the global role of protozoa at the ruminal or animal levels. It is now generally considered that a presence of an abundant protozoal population in the rumen has a negative effect on the amino acid (AA) supply to ruminants and contribute to generate more methane but, nevertheless, protozoa must not be considered as parasites. They are useful for numerous reasons. They stabilise rumen pH when animal are fed diets rich in available starch and decrease the redox potential of rumen digesta. Because cellulolytic bacteria are very sensitive to these two parameters, protozoa indirectly stimulate the bacterial cellulolytic activity and supply their own activity to the rumen microbial ecosystem. They could also supply some peptides in the rumen medium which can stimulate the growth of the rumen microbiota, but this aspect has never been considered in the past. Their high contribution to ammonia production has bad consequences on the urinary nitrogen excretion but means also that less dietary soluble nitrogen is necessary when protozoa are present. Changes in the molar percentages of VFA and gases from rumen fermentations are not so large that they could alter significantly the use of energy by animals. The answer of animals to elimination of protozoa (defaunation) depends on the balance between energy and protein needs of animals and the supply of nutrients supplied through the diet. Defaunation is useful in case of diets short in protein nitrogen but not limited in energy supply for animals having high needs of proteins.

Advanced Treatment of Wastewater Using Symbiotic Co-culture of Microalgae and Bacteria (미세조류와 박테리아의 공생 배양을 이용한 하폐수 고도처리)

  • Mujtaba, Ghulam;Lee, Kisay
    • Applied Chemistry for Engineering
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    • v.27 no.1
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    • pp.1-9
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    • 2016
  • The co-culture system of microalgae and bacteria enables simultaneous removal of BOD and nutrients in a single reactor if the pair of microorganisms is symbiotic. In this case, nutrients are converted to biomass constituents of microalgae. This review highlights the importance and recent researches using symbiotic co-culture system of microalgae and bacteria in wastewater treatment, focusing on the removal of nitrogen and phosphorus. During wastewater treatment, the microalgae produces molecular oxygen through photosynthesis, which can be used as an electron acceptor by aerobic bacteria to degrade organic pollutants. The released $CO_2$ during the bacterial mineralization can then be consumed by microalgae as a carbon source in photosynthesis. Microalgae and bacteria in the co-culture system could cooperate or compete each other for resources. In the context of wastewater treatment, positive relationships are prerequisite to accomplish the sustainable removal of nutrients. Therefore, the selection of compatible species is very important if the co-culture has to be utilized in wastewater treatment.

Bioaugmentation with GFP-Tagged Pseudomonas migulae AN-1 in Aniline-Contaminated Aquifer Microcosms: Cellular Responses, Survival and Effect on Indigenous Bacterial Community

  • Zhao, Yongsheng;Qu, Dan;Zhou, Rui;Ma, Yunge;Wang, Hao;Ren, Hejun
    • Journal of Microbiology and Biotechnology
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    • v.26 no.5
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    • pp.891-899
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    • 2016
  • The recently isolated aniline-degrading bacterium Pseudomonas migulae AN-1 was tagged with green fluorescent protein (GFP) to investigate its bioaugmentation potential against aniline-contaminated groundwater through microcosm experiments. The survival and cellular response of GFP-tagged AN-1 introduced in a lab-scale aquifer corresponded directly with aniline consumption. During the process, the GFP-tagged AN-1 biomass increased from 7.52 × 105 cells/ml to 128 × 105 cells/ml and the degradation rate of aniline was 6.04 mg/l/h. GFP-tagged AN-1 was moderately hydrophobic (41.74%-47.69%) when treated with 20-100 mg/l aniline and exhibited relatively strong hydrophobicity (55.25%-65.78%) when the concentration of aniline was ≥100 mg/l. The membrane permeability of AN-1 increased followed by a rise in aniline below 100 mg/l and was invariable with aniline above 100 mg/l. Pyrosequencing analysis showed that the relative abundance of Proteobacteria (accounted for 99.22% in the non-bioaugmentation samples) changed to 89.23% after bioaugmentation with GFP-tagged AN-1. Actinobacteria increased from 0.29% to 2.01%, whereas the abundance of Firmicutes barely changed. These combined findings demonstrate the feasibility of removing aniline in aquifers by introducing the strain AN-1 and provide valuable information on the changes in the diversity of dominant populations during bioaugmentation.

Influence of Pulsed Electric Field on Accumulation of Calcium in Lactobacillus rhamnosus B 442

  • Goral, Malgorzata;Pankiewicz, Urszula;Sujka, Monika;Kowalski, Radoslaw;Giral, Dariusz;Kozlowicz, Katarzyna
    • Journal of Microbiology and Biotechnology
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    • v.30 no.1
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    • pp.44-53
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    • 2020
  • Calcium is an element that performs many important functions in the human body. A study was conducted on the use of a pulsed electric field (PEF) to enrich cells of Lactobacillus rhamnosus B 442 in calcium ions. The highest concentration of calcium ions in bacterial cells (7.30 mg/g d.m.) was obtained at ion concentration of 200 ㎍/ml of medium and with the use of the following PEF parameters: field strength 3.0 kV/cm, exposure time 10 min, pulse width 75 ms and 20 h of culturing after which bacteria were treated with the field. Cell biomass varied in the range from 0.09 g/g d.m. to 0.252 g/g d.m., and the total number of bacteria ranged from 1010 CFU/ml to 1012 CFU/ml. Microscope photographs prove that calcium ions were situated within the cells of the bacteria, and electroporation contributed to an increase in the effectiveness of the ion bioaccumulation process. Samples containing calcium and subjected to electroporation displayed intensive fluorescence. The significance of this research was the possibility of using probiotic bacteria enriched with calcium ions for the production of functional food in subsequent studies.

Responses of Benthic Animals in Spatial Distribution to the Sedimentary Environments on the Deep-sea Floor, the Clarion-Clipperton Fracture Zone, Northeastern Pacific Ocean (북동 태평양 심해저 C-C 해역의 퇴적 환경과 대형저서동물 분포와의 관계)

  • Park, Heung-Sik;Chi, Sang-Bum;Paik, Sang-Kyu;Kim, Woong-Seo
    • Ocean and Polar Research
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    • v.26 no.2
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    • pp.311-321
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    • 2004
  • Relationships between sedimentary environments and abundance of benthic animals were examined on the deep-sea floor, the Clarion-Clipperton Fracture Zone, in the northeast equatorial Pacific Ocean. Specimens were collected using a box corer at 8 stations by sieving through 0.3 mm mesh screen. Sediments showed finer grain size ranged from 5.63 to $7.97{\varphi}$, 83.1% of mean porosity, 1.81 kPa of mean shear strength and organic carbon content in sediment ranged from 0.97 to $1.87\;mg/cm^3$. Manganese nodules covered on the bottom layer from 4 to 57% of coverages. A total of 26 faunal groups in 6 phyla was sampled and comprised 1,467 individuals. Mean biomass were calibrated to 0.5 gWWt/$0.06\;m^2$. Small-sized animals including foraminiferans and nematods were dominated among the faunal group which comprised 49.1% (892 ind.) and 11.5% (320 ind.), respectively. In SPI-analysis, vertical bio-disturbance marks were not observed except to Beggiatoa-type bacterial mats. As the results of relationship between environments and benthos, abundance of benthic animals, especially nematode, showed only a negative correlation to the coverage of nodules, and any other sedimentary factors analyzed in this study were rarely affected to the spatial distribution of benthic animals.

Utilization of Potato Starch Processing Wastes to Produce Animal Feed with High Lysine Content

  • Li, Ying;Liu, Bingnan;Song, Jinzhu;Jiang, Cheng;Yang, Qian
    • Journal of Microbiology and Biotechnology
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    • v.25 no.2
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    • pp.178-184
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    • 2015
  • This work aims to utilize wastes from the potato starch industry to produce single-cell protein (SCP) with high lysine content as animal feed. In this work, S-(2-aminoethyl)-L-cysteine hydrochloride-resistant Bacillus pumilus E1 was used to produce SCP with high lysine content, whereas Aspergillus niger was used to degrade cellulose biomass and Candida utilis was used to improve the smell and palatability of the feed. An orthogonal design was used to optimize the process of fermentation for maximal lysine content. The optimum fermentation conditions were as follows: temperature of 40℃, substrate concentration of 3%, and natural pH of about 7.0. For unsterilized potato starch wastes, the microbial communities in the fermentation process were determined by terminal restriction fragment length polymorphism analysis of bacterial 16S rRNA genes. Results showed that the dominant population was Bacillus sp. The protein quality as well as the amino acid profile of the final product was found to be significantly higher compared with the untreated waste product at day 0. Additionally, acute toxicity test showed that the SCP product was non-toxic, indicating that it can be used for commercial processing.

A Fosmid Cloning Strategy for Detecting the Widest Possible Spectrum of Microbes from the International Space Station Drinking Water System

  • Choi, Sangdun;Chang, Mi Sook;Stuecker, Tara;Chung, Christine;Newcombe, David A.;Venkateswaran, Kasthuri
    • Genomics & Informatics
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    • v.10 no.4
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    • pp.249-255
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    • 2012
  • In this study, fosmid cloning strategies were used to assess the microbial populations in water from the International Space Station (ISS) drinking water system (henceforth referred to as Prebiocide and Tank A water samples). The goals of this study were: to compare the sensitivity of the fosmid cloning strategy with that of traditional culture-based and 16S rRNA-based approaches and to detect the widest possible spectrum of microbial populations during the water purification process. Initially, microbes could not be cultivated, and conventional PCR failed to amplify 16S rDNA fragments from these low biomass samples. Therefore, randomly primed rolling-circle amplification was used to amplify any DNA that might be present in the samples, followed by size selection by using pulsed-field gel electrophoresis. The amplified high-molecular- weight DNA from both samples was cloned into fosmid vectors. Several hundred clones were randomly selected for sequencing, followed by Blastn/Blastx searches. Sequences encoding specific genes from Burkholderia, a species abundant in the soil and groundwater, were found in both samples. Bradyrhizobium and Mesorhizobium, which belong to rhizobia, a large community of nitrogen fixers often found in association with plant roots, were present in the Prebiocide samples. Ralstonia, which is prevalent in soils with a high heavy metal content, was detected in the Tank A samples. The detection of many unidentified sequences suggests the presence of potentially novel microbial fingerprints. The bacterial diversity detected in this pilot study using a fosmid vector approach was higher than that detected by conventional 16S rRNA gene sequencing.

Effect of Temperature on Water Quality Improvement of Natural Plant-Mineral Composites (PMC) in a Eutrophic Lake, Lake Shingal, Korea (부영양 신갈지에서 천연물질 혼합제(PMC)의 수질개선능: 현장수온의 영향)

  • Byun, Jung-Hwan;Hwang, Su-Ok;Mun, Sun-Ki;Hwang, Soon-Jin;Kim, Baik-Ho
    • Korean Journal of Ecology and Environment
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    • v.46 no.2
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    • pp.225-233
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    • 2013
  • We examined the effect of different field temperatures on water quality improvement (WQI) of natural domestic plant-mineral composites (PMCs). This method was previously used by Kim et al. (2011), to monitor the restoration of water quality of a eutrophic lake, Lake Shingal (Korea). Results indicate that PMCs on phytoplankton, BOD and phosphorus showed more than 70% WQI below $20^{\circ}C$, and less than 40% WQI over $25^{\circ}C$, respectively. The WQIs of PMCs on blue-green algae were gradually decreased with the increase of temperature, whilst diatoms exhibited more than 90% higher WQIs, regardless of water temperature. Additionally, the WQIs on bacterial biomass and total nitrogen were low at all temperatures. These results collectively indicate that water quality improvement activity of plant-mineral composites was dependent on the water temperature, and that the field application of above chemical during temperatures over $25^{\circ}C$, would be less effective in treating a cyanobacteria bloom dominated by Microcystis aeruginosa, than by diatoms.