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Variation of Anthocyanin and Protein Contents in Glycine max L. (Merr) (Soybean) Germplasms from Korea

  • Choi, Yu Mi;Lee, Sukyeung;Hyun, Do-Yoon;Ko, Ho-Cheol;Rho, Nayoung;Hur, On-Sook;Yoon, Hyemyeong;Lee, Myung-Chul;Oh, Sejong;Shin, Myoung-Jae;DESTA, Kebede Taye
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.10a
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    • pp.13-13
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    • 2019
  • Soybean (Glycine max L. (Merr) is commonly consumed and found in major foods including soymilk, soy sauce, tofu, and soy sprout in Korea and east Asia. In addition, it is common to cook the whole seeds with rice. Soybean is known to have ranges of health benefits including antiaging, anticancer, neuroprotective and antidiabetic taken either as supplement or dietary food. Anthocyanins and flavonoids in G. max are found to be the main contributors to such wide arrays of health benefits. Due to increasing economic values of soybean, development of specialty soybean cultivars is becoming an area of interest worldwide. In this study, 746 black soybean accessions from National Agrobiodiversity Center were characterized as part of an attempt to identify important germplasms of G. max. Seed coats of each accession were analyzed for their total anthocyanin, cyanidin 3-O-Glucoside (C-3-O-G), delphinidin 3-O-glucoside (D-3-O-G), petunidin-3-O-glucoside (Pt-3-O-G), and their whole seeds for crude protein contents. HPLC was used to determine and quantify the anthocyanin compositions while crude protein was determined using Kjeldahl method by Kjeltec auto-analyzer (Kjeltec 8400, Foss, Sweden). Accessions were grouped according to their anthocyanins and protein contents; the mean content of which were correlated to agronomic traits including maturity date, one hundred seed weight, cotyledon color and seed lust color. The results indicated that the total anthocyanin content (TAC) ranged from 273.77 to 6250.52 mg/100 g, with mean value of 1853.03 mg/100 g while the crude protein content (CPC) being between 33.43 and 47.51%, with mean value of 40.81%. The highest number of accessions (45.97%) showed TAC between 1000~1900 mg/100 g while 30.96% of accessions showed CPC between 41~43%. Among the 746 accessions considered, 11 (IT142935, 175818, 175855, 177191, 177209, 177211, 177214, 177216, 177218, 177220, 177274) of them showed TAC above 4000 mg/100 g. C-3-O-G was found to be the major contributor to TAC showing strong correlation. Accessions with green cotyledon color showed high mean TAC compared to those having yellow cotyledon color, and accessions with dull seed lust color showed high mean TAC than those having shiny seed lust color. One hundred seeds weight and maturity date showed positive correlation with all anthocyanin contents, except for Pt-3-O-G in the latter case. The overall result of the present study could be used as background for developing new black soybean cultivars and breeds with high anthocyanin and protein contents. The result depicted that many of the accessions could be used as potential parental lines.

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Changes in the glucose and insulin responses according to high-protein snacks for diabetic patients

  • Yang, Junyoung;Park, Hae Jin;Hwang, Wonsun;Kim, Tae Ho;Kim, Hyeonmok;Oh, Jieun;Cho, Mi Sook
    • Nutrition Research and Practice
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    • v.15 no.1
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    • pp.54-65
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    • 2021
  • BACKGROUND/OBJECTIVES: This study aimed to develop healthy, appetizing high-protein snacks with enhanced isolated soy protein for diabetic patients and determine the blood glucose and insulin response after being consumed by these patients. MATERIALS/METHODS: Thirty adult patients aged between 30 and 75 years, with a ≤ 10-year history of type 2 diabetes and hemoglobin A1c of < 7.5%, were enrolled in this study. They made 3 clinical visits at one-week intervals. The control group consumed 50 g carbohydrates (white bread), whereas the test groups consumed high-protein grain (HP_G) or high-protein chocolate (HP_C) after an 8-hrs fast. Blood (2 ㎤) was drawn at 15, 30, 45, 60, 90, and 120 min before and after consumption to analyze the blood glucose and insulin concentrations. RESULTS: Compared to the commercial snacks, the developed high-protein snacks had below-average calorie, carbohydrate, and fat content and a 2.5-fold higher protein content. In diabetic patients who consumed these snacks, the postprandial blood glucose increased between 15 min and 2 h after consumption, which was significantly slower than the time taken for the blood glucose to increase in the patients who consumed the control food product (P < 0.001). Insulin secretion was significantly lower at 45 min after consumption (P < 0.05), showing that the high-protein snacks did not increase the blood glucose levels rapidly. The incremental area under the curve (iAUC), which indicated the degree of blood sugar and insulin elevation after food intake, was higher in the control group than the groups given the 2 developed snacks (P < 0.001), and there was no significant difference in insulin secretion. CONCLUSIONS: The results of the postprandial blood glucose and insulin response suggest that high-protein snacks are potential convenient sources of high-quality protein and serve as a healthier alternative for patients with type 2 diabetes, who may have limited snack product choices. Such snacks may also provide balanced nutrition to pre-diabetic and obese individuals.

The Association between Mortality and the Oxygen Saturation and Fraction of Inhaled Oxygen in Patients Requiring Oxygen Therapy due to COVID-19-Associated Pneumonia

  • Choi, Keum-Ju;Hong, Hyo-Lim;Kim, Eun Jin
    • Tuberculosis and Respiratory Diseases
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    • v.84 no.2
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    • pp.125-133
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    • 2021
  • Background: The coronavirus disease (COVID-19) can manifest in a range of symptoms, including both asymptomatic systems which appear nearly non-existent to the patient, all the way to the development of acute respiratory distress syndrome (ARDS). Specifically, COVID-19-associated pneumonia develops into ARDS due to the rapid progression of hypoxia, and although arterial blood gas analysis can assist in halting this deterioration, the current environment provided by the COVID-19 pandemic, which has led to an overall lack of medical resources or equipment, has made it difficult to administer such tests in a widespread manner. As a result, this study was conducted in order to determine whether the levels of oxygen saturation (SpO2) and the fraction of inhaled oxygen (FiO2) (SF ratio) can also serve as predictors of ARDS and the patient's risk of mortality. Methods: This was a retrospective cohort study conducted from February 2020 to Mary 2020, with the study's subjects consisting of COVID-19 pneumonia patients who had reached a state of deterioration that required the use of oxygen therapy. Of the 100 COVID-19 pneumonia cases, we compared 59 pneumonia patients who required oxygen therapy, divided into ARDS and non-ARDS pneumonia patients who required oxygen, and then investigated the different factors which affected their mortality. Results: At the time of admission, the ratios of SpO2, FiO2, and SF for the ARDS group differed significantly from those of the non-ARDS pneumonia support group who required oxygen (p<0.001). With respect to the predicting of the occurrence of ARDS, the SF ratio on admission and the SF ratio at exacerbation had an area under the curve which measured to be around 85.7% and 88.8% (p<0.001). Multivariate Cox regression analysis identified that the SF ratio at exacerbation (hazard ratio [HR], 0.916; 95% confidence interval [CI], 0.846-0.991; p=0.029) and National Early Warning Score (NEWS) (HR, 1.277; 95% CI, 1.010-1.615; p=0.041) were significant predictors of mortality. Conclusion: The SF ratio on admission and the SF ratio at exacerbation were strong predictors of the occurrence of ARDS, and the SF ratio at exacerbation and NEWS held a significant effect on mortality.

Seasonal Concentration of Polycyclic Aromatic Hydrocarbons (PAHs) in Residential Areas Around Petrochemical Complexes and Risk Assessment Using Monte-Carlo Simulation (석유화학단지 주변 주거지역 다환방향족탄화수소(PAHs)의 농도와 Monte-Carlo 모의실험을 통한 위해성평가)

  • Park, Dong-Yun;Choe, Young-Tae;Yang, Wonho;Choi, Kil-Yong;Lee, Chae-Kwan
    • Journal of Environmental Health Sciences
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    • v.47 no.4
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    • pp.366-377
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    • 2021
  • Background: Polycyclic aromatic hydrocarbons (PAHs) are generated in petrochemical complexes, can spread to residential areas and affect the health of residents. Although harmful PAHs are mainly present in particle phase, gas phase PAHs can generate stronger toxic substances through photochemical reaction. Therefore, the risk assessment for PAHs around the petrochemical complex should consider both particle and gas phase concentrations. Objectives: This study aimed to investigate the concentration characteristics of particle and gas phase PAHs by season in residential areas around petrochemical complexes, and to assess the risk of PAHs. Methods: Samples were collected for 7 days by seasons in 2014~2015 using a high volume air sampler. Particle and gas phase PAHs were sampled using quartz filter and polyurethane foam, respectively, analyzed by GC-MS. Chronic toxicity and probabilistic risk assessment were performed on 14 PAHs. For chronic toxicity risk assessment, inhalation unit risk was used. Monte-Carlo simulation was performed for probabilistic risk assessment using the mean and standard deviation of measured PAHs. Results: The concentration of particle total PAHs was highest in autumn. The gas phase concentration was highest in autumn. The average gas phase distribution ratio of low molecular weight PAHs composed of 2~3 benzene rings was 85%. The average of the medium molecular weight composed of 4 benzene rings was 53%, and the average of the high molecular weight composed of 5 or more benzene rings was 9%. In the chronic toxicity risk assessment, 7 of the 14 PAHs exceeded the excess carcinogenic risk of 1.00×10-6. In the Monte-Carlo simulation, Benzo[a]pyrene had the highest probability of exceeding 1.00×10-6, which was 100%. Conclusions: The concentration of PAHs in the residential area around the petrochemical complex exceeded the standard, and the excess carcinogenic risk was evaluated to be high. Therefore, it is necessary to manage the air environment around the petrochemical complex.

A Study on the View Characteristics of the Pavilion around Gyeongpo Lake (경포호 누정의 조망특성에 관한 연구)

  • Han, Gab-Soo
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.39 no.4
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    • pp.66-76
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    • 2021
  • The purpose of this study was to analyze the transition process of Gyeongpo lake and to analyze the viewing characteristics of each period in the pavilion. The view characteristics at pavilion were analyzed by overlapping classification maps of landscape types by period with the view range map from pavilion. The circumference and area of Gyeongpo lake have been reduced to 15.2km and 3.9km2 in the 1750s, 9.0km and 1.7km2 in the 1933, and now 4.41km and 1.0km2. By period, the largest decrease in the proportion of Gyeongpo lake viewed from Pavilion was Hwanseonjeong, which decreased by 48.0% from the 1750 to 2017. Hwallaejeong, Haeunjeong, and Hohaejeong were found to be impossible to view Gyeongpo lake as some of the adjacent lakes were changed to agricultural land. Pavilion has great significance as a space for traditional culture created in the background of humanities and natural environments. For the succession and development of Pavilion culture, it is necessary to use it as a cultural resource, to continuously manage the building, and to partially restore Gyeongpo lake considering the function of each pavilion.

Does dexmedetomidine combined with levobupivacaine in inferior alveolar nerve blocks among patients undergoing impacted third molar surgery control postoperative morbidity?

  • Patil, Shweta Murlidhar;Jadhav, Anendd;Bhola, Nitin;Hingnikar, Pawan;Kshirsagar, Krutarth;Patil, Dipali
    • Journal of Dental Anesthesia and Pain Medicine
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    • v.22 no.2
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    • pp.145-153
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    • 2022
  • Background: Postoperative analgesia (POA) is an important determinant of successful treatment. Dexmedetomidine (DEX) has recently gained attention as a promising adjuvant to local anesthetics (LA). The present study aimed to evaluate the efficacy and safety of levobupivacaine (LB) as an adjuvant during inferior alveolar nerve block (IANB) in the extraction of lower impacted third molars (LITM). Methods: A prospective, randomized, placebo-controlled, triple-blind, parallel-arm, and clinical study was performed on 50 systemically healthy participants who required removal of an asymptomatic LITM. Using a 1:1 distribution, the participants were randomized into two groups (n = 25). Group L (control group) received 1.8 mL of 0.5% LB and 0.2 mL normal saline (placebo) and Group D (study group) received a blend of 1.8 mL of 0.5% LB and 0.2 mL (20 ㎍) DEX. The primary outcome variable was the duration of POA and hemodynamic stability, and the secondary variable was the total number of analgesics required postoperatively for up to 72 h. The participants were requested to record the time of rescue analgesic use and the total number of rescue analgesics taken. The area under the curve was plotted for the total number of analgesics administered. The pain was evaluated using the visual analog scale. Data analysis was performed using paired students and unpaired t-test, Mann-Whitney U test, Chi-square test, and receiver operating characteristic analysis. Statistical significance was set at P < 0.05. Results: The latency, profoundness of anesthesia, and duration of POA were statistically significant (P < 0.05). The differences between mean pain scores at 6, 12, 24, 48, and 72 h were found to be significant (each P = 0.0001). Fewer analgesics were required by participants in group D (2.12 ± 0.33) than in L (4.04 ± 0.67), with a significant difference (P = 0.0001). Conclusion: Perineurally administered LA with DEX is a safe, effective, and therapeutic approach for improving latency, providing profound POA, and reducing the need for postoperative analgesia.

The effect of self-regulation ability on fear of dental treatment among male high school students (남자 고등학생의 자기조절능력이 치과 치료공포감에 미치는 영향)

  • Kim, Young-Sun;Lee, Jung-Hwa
    • Journal of Korean Dental Hygiene Science
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    • v.4 no.2
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    • pp.89-98
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    • 2021
  • Background: To analyze the effect of self-regulation on the fear of dental treatment in order to use it as basic data for efficient oral health care among male adolescents. Methods: A survey was administered to 241 first graders of specialized high schools in Daegu using a self-entry method. Twenty questions about fear of dental treatment and fifteen questions about self-regulation were included. The higher the score, the higher the fear of dental treatment and the self-regulation ability, showing a high reliability of 0.972 and 0.750, respectively. Results: The mean score of the participants' self-regulation ability was 3.25±0.51 and that of the degree of fear for dental treatment was relatively low at 1.72±0.85. It was confirmed that the higher the academic performance (F=7.635, p<0.01), the better was the self-regulation based on self-diagnosis (F=3.142, p<0.05), and was associated with higher health awareness (F=5.894, p<0.01). The degree of fear for dental treatment was significantly higher in the self-diagnosis-induced poor health group (F=4.933, p<0.01) and associated with a lower awareness of health (F=3.093, p<0.05). The participants' self-regulation ability was significantly negatively correlated with the degree of fear regarding dental treatment (r=-0.269, p<0.01). Regression analysis was performed using the degree of fear as a dependent variable and including sub-area variables of self-diagnosis-based health status, perception of health, and self-regulation ability as independent variables (β=-0.163, p<0.05). Among the self-regulation abilities, controlled composition (β=-0.232, p<0.01) had a significant effect; the higher the composition, the lower the degree of fear. The total explanatory power of this variable was 8.5%. Conclusion: An appropriate customized education program that can encourage individuals to practice self-care and maintain oral hygiene along with a clear understanding of underlying individual oral health conditions during adolescence is essential for promoting oral health.

Accuracy evaluation of domestic and foreign land cover spectral libraries using hyperspectral image (초분광 영상을 활용한 국내외 토지피복 분광 라이브러리 정확도 평가)

  • Park, Geun Ryeol;Lee, Geun-Sang;Cho, Gi-Sung
    • Journal of Cadastre & Land InformatiX
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    • v.51 no.2
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    • pp.169-184
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    • 2021
  • Recently, land cover spectral libraries have been widely used in studies to classify land cover based on hyperspectral images. Overseas, various institutions have built and provided land cover spectral libraries, but in Korea, the building and provision of land cover spectral libraries is insufficient. Against this background, the purpose of this study is to suggest the possibility of using domestic and foreign spectral libraries in the classification studies of domestic land cover. Band matching is required for comparative analysis of the spectral libraries and land cover classification using the spectral libraries, and in this study, an automation logic to automatically perform this is presented. In addition, the directly constructed domestic land cover spectral library and the existing overseas land cover spectral library were comparatively analyzed. As a result, the directly constructed land cover spectral library had the highest correlation coefficient of 0.974. Finally, for the accuracy evaluation, aerial hyperspectral images of the study area were supervised and classified using the domestic and foreign land cover spectral libraries using the SAM technique. As a result of the accuracy evaluation, it is judged that Soils, Artificial Materials, and Coatings among the classification items of the foreign land cover spectral library can be sufficiently applied to classify the cover in Korea.

Investigating Professional Competency and the Needs of Training for Occupational Therapists Using Sensory Integration Interventions (감각통합중재를 사용하는 작업치료사의 실무 역량에 대한 인식 및 교육 요구도)

  • Jung, Hyerim;Lee, Ji-Hyun
    • The Journal of Korean Academy of Sensory Integration
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    • v.20 no.1
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    • pp.26-38
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    • 2022
  • Objective : The aim of this study was to determine the priority competency by analyzing the importance, performance, and educational requirements of occupational therapists to develop a competency in performing sensory integration intervention. Methods : Occupational therapists were surveyed by gender, age, educational background, work location, occupational therapy experience, and sensory integration therapy experience. The difference was investigated through the importance-performance analysis of competency, and the priority of the competency was investigated using the Borich demand analysis method. Results : The therapists recognized professional competency as the most important, whereas performance was the least important. In all sub-competencies, the importance was high, but the performance was low. As a result, the education requirement was highest for professional competency. The importance-performance analysis revealed that professional competency was the area requiring the most urgent improvement. As a result of the Borich demand analysis, statistically significant differences between the importance of all competencies and the actual performance. The most significant difference was found in the professional competency group. Conclusion : The occupational therapists in this study who performed sensory integration interventions recognized professional competency as the most important but most lacking in actual clinical practice. The results of this study will be used as guidelines for developing competency-based sensory integrated intervention curricula.

The Sexual Health for the Elderly in the Characteristics and Factors: Based on the WHO Sexual Health (WHO 성 건강에 근거한 노인 성 건강 특성과 영향요인)

  • Ryu, JiHye;Kang, ChangHyun
    • 한국노년학
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    • v.41 no.1
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    • pp.69-83
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    • 2021
  • The purpose of this study is to identify the characteristics and factors of each area by considering the gender health of the elderly as the integration of mental, physical, emotional, and social gender health based on the WHO gender health. The sexual health of the elderly is significantly different by gender, academic background, and region, and there is a clear difference in mental, physical, emotional sexual health and social sexual health. The mental, physical and emotional sexual health of the elderly in urban areas, those with high education and male is high, but the elderly in the rural areas, those with low education, and female are high in social sexual health. The factors affecting sexual health are as follows. Mental sexual health was positively influenced by satisfaction of sexual life and sexual knowledge. Physical sexual health was positively influenced by life and spouse satisfaction, and emotional sexual health was positively influenced by sexual relationship, spouse satisfaction, sexual life satisfaction and sexual knowledge. Social sexual health has a negative (-) effect on spouse satisfaction and sexual relationships. Integrated sexual health in old age can affect subjective health and quality of life, leading to successful aging. Sexual health education is needed to form a safe and healthy sexual life and interpersonal relationship. The 'sex' that appears as a cumulative trajectory of life should be accompanied by the accurate sexual health awareness and sex education that encompasses all generations. Therefore, in the composition of sex health education program, it is necessary to reflect the components that can enhance the sensitivity of adulthood to strengthen understanding and communication of spouse.