• Title/Summary/Keyword: Back-extraction

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Fatty Acids and Protein Recovery of Squid Viscera with Supercritical Carbon Dioxide

  • Park, Ji-Yeon;Back, Sung-Sin;Chun, Byung-Soo
    • Journal of Marine Bioscience and Biotechnology
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    • v.1 no.3
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    • pp.206-212
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    • 2006
  • Supercritical carbon dioxide ($SCO_2$) extraction was investigated as a method for protein-sourcing material from squid viscera. To find the optimum conditions, the extraction of squid viscera using $SCO_2$ was performed under the conditions of temperature range from 35 to $45^{\circ}C$ and constant pressure 25 MPa using Hewlett-Packard 7680T. Also from result of SDS-PAGE, the protein denaturation was minimized when using $SCO_2$ extraction. And the major amino acids in the squid viscera were glutamic acid, aspartic acid, lysine, leucine, arginine, alanine, glycine, isoleucine, and valine. The main fatty acids from squid viscera were myristic acid, palmitic acid, stearic acid, heneicosanoic acid, palmitoleic acid, elaidic acid, oleic acid, eicosenoic acid, EPA (eicosapentaenoic acid), and DHA (docosahexaenoic acid).

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GC-FID Analysis of Tranylcypromine in Rat Urine (GC-FID에 의한 Rat 뇨중 Tranylcypromine의 분석)

  • 강건일;전순영
    • YAKHAK HOEJI
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    • v.29 no.5
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    • pp.260-267
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    • 1985
  • A gas chromatography with flame ionization detection was developed to measure tranylcypromine in rat urine. The method involves extraction of the drug and the internal standard, phenylpropylamine from the urine using ethyl acetate and back extraction into 0.5N $H_{2}SO_{4}$. Following final extraction using dichloromethane, both the drug and the internal standard were converted to trifluoroacetyl derivatives and analyzed using a column of 3% SE-30 on 80/100 mesh Chromosorb W(HP). A calibration curve was constructed in the range of $5~50{\mu}g$tranylcypromine sulfate in 0.5ml urine and found to be linear. The detection limit was $2{\mu}g$. The tranylcypromine could be analyzed with the percent recovery of $100.81{\pm}8.13$ (SD) ina concentration range of $8-40{\mu}g$ in 0.5ml urine. When 0.4mmol/kg dose of the drug was administered through, an oral route, excretion percent of tranylcypromine in rat urine over 36hr was found to be $11.90{\pm}6.04$ (SD) for tranyleypromine sulfate and $2.23{\pm}0.63$ (SD) for benzyl trans-2-phenylcyclopropanecarbamate.

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Optimal Fuzzy Sliding-Mode Control for Microcontroller-based Microfluidic Manipulation in Biochip System

  • Chung, Yung-Chiang;Wen, Bor-Jiunn
    • 제어로봇시스템학회:학술대회논문집
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    • 2004.08a
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    • pp.196-201
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    • 2004
  • In biometric and biomedical applications, a special transporting mechanism must be designed for the ${\mu}$TAS (micro total analysis system) to move samples and reagents through the microchannels that connect the unit procedure components in the system. An important issue for this miniaturization and integration is microfluid management technique, i.e., microfluid transportation, metering, and mixing. In view of this, this study presents an optimal fuzzy sliding-mode control (OFSMC) design based on the 8051 microprocessor and implementation of a complete microfluidic manipulated system implementation of biochip system with a pneumatic pumping actuator, a feedback-signal photodiodes and flowmeter. The new microfluid management technique successfully improved the efficiency of molecular biology reaction by increasing the velocity of the target nucleic acid molecules, which increases the effective collision into the probe molecules as the target molecules flow back and forth. Therefore, this hybridization chip was able to increase hybridization signal 6-fold and reduce non-specific target-probe binding and background noises within 30 minutes, as compared to conventional hybridization methods, which may take from 4 hours to overnight. In addition, the new technique was also used in DNA extraction. When serum existed in the fluid, the extraction efficiency of immobilized beads with solution flowing back and forth was 88-fold higher than that of free-beads.

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A Development of GUI System for Optimal Operational Scheduling on Industrial Cogeneration Systems Using Evolutionary Algorithms (산업체 열병합발전시스템에서 최적운전계획 수립을 위한 진화 알고리즘을 이용한 GUI System 개발)

  • Jeong, Ji-Hoon;Lee, Jong-Beom
    • The Transactions of the Korean Institute of Electrical Engineers A
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    • v.51 no.11
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    • pp.544-550
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    • 2002
  • This paper describes a strategy of a daily optimal operational scheduling on the industrial cogeneration system. The cogeneration system selected to establish the scheduling consists of three units and several auxiliary devices which include three auxiliary boilers, t재 waste boilers and three sludge incinerators. One unit generated electrical and thermal energy using the back pressure turbine. The other two units generate the energy using the extraction condensing turbine. Three auxiliary devices operate to supply energy to the loads with three units. The cogeneration system is able to supply enough the thermal energy to the thermal load, however it can not sufficiently supply the electric energy to the electrical load. Therefore the insufficient electric energy is compensated by buying electrical energy from utility. In this paper, the evolutionary algorithms was applied to establish the optimal scheduling for the cogeneration systems. Also the GUI System was developed using established mathematics medeling and evolutionary algorithms in order that non-experts are able to establish operational scheduling. This results revel that the proposed modeling and strategy can be effectively applied to cogeneration system for paper mill.

A Rational Operation Scheduling Using Evolutionary Algorithm on Industrial Cogeneration System (산업용 열병합발전시스템에서 진화 알고리즘을 이용한 합리적 운전계획 수립에 관한 연구)

  • Choi, Kwang-Beom;Jeong, Ji-Hoon;Lee, Jong-Beom
    • The Transactions of the Korean Institute of Electrical Engineers A
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    • v.49 no.10
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    • pp.494-501
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    • 2000
  • This paper describes a strategy of a daily optimal operational scheduling in cogeneration system for paper mill. The cogeneration system selected to establish the scheduling consists of three units and several auxiliary devices. One unit generates electrical and thermal energy using the back pressure turbine. The rest two units generate the energy using the extraction condensing turbine. Three auxiliary boilers, two waste boilers and three sludge incinerators operate to supply energy to the loads with three units. The cogeneration system is able to supply enough the thermal energy to the thermal load, however it can not sufficiently supply the electrical power to the electrical load. Therefore the insufficient electric energy is compensated by buying electrical energy from utility. When the operational scheduling is performed considering the environmental problem. This paper shows the simulation results for daily operational scheduling obtained using the evolutionary algorithm. This results reveal that the proposed modeling and strategy can be effectively applied to cogeneration system for paper mill.

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Optimal Operation of industrial Cogeneration Plant with Back-Pressure and Extraction-Condensing Turbine/Generators (背壓과 抽氣復水터빈을 採用한 産業用 熱倂合 發電플랜트의 最適運用)

  • 오성근
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.12 no.2
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    • pp.69-76
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    • 1998
  • This paper presents a novel algorithm for determining the optimal operation of a cogeneration plant with back-pressure and extraction-condensing turbine/generators. The proposed algorithm determines the optimum load of boilers and turbine/generators, using only one parameter, the steam mass flow rate, which can be obtained directly from on-line measurement during plant operation. The proposed algorithm consists of the non -linear operating cost function, and its correlated constraints. Furthermore, it has been successfully applied to an actual industrial cogeneration plant, with satisfactory results. Comparison of these results with actual operating data has revealed that using the proposed algorithm results in at least 1.2~4.5[%] operating cost saving, depending on the process steam load. Furthermore the proposed algorithm can be easily installed in a process control computer because the required input data can be easily obtained from information available on-line.n-line.

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Optical Characterization of Light-Emitting Diodes Grown on the Cylinder Shape 300 nm Diameter Patterned Sapphire Substrate (300 nm Diameter Cylinder-Shape 나노패턴 기판을 이용한 LEDs의 광학적 특성)

  • Kim, Sang Mook;Kim, Yoon Seok
    • Korean Journal of Materials Research
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    • v.29 no.1
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    • pp.59-64
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    • 2019
  • This study investigates the optical characteristics of InGaN multiple quantum wells(MQWs) light emitting diodes(LEDs) on planar sapphire substrates(PSSs), nano-sized PSS(NPSS) and micro-sized PSS(MPSS). We obtain the results as the patterning size of the sapphire substrates approach the nanometer scale: The light from the back side of the device increases and the total light extraction becomes larger than the MPSS- and planar-LEDs. The experiment is conducted by Monte Carlo ray-tracing, which is regarded as one of the most suitable ways to simulate light propagation in LEDs. The results show fine consistency between simulation and measurement of the samples with different sized patterned substrates. Notably, light from the back side becomes larger in the NPSS LEDs. We strongly propose that the increase in the light intensity of NPSS LEDs is due to an abnormal optical distribution, which indicates an increase of extraction probability through NPSS.

Crack Size Determination Through Neural Network Using Back Scattered Ultrasonic Signal (저면산란 초음파 신호 및 신경회로망을 이용한 균열크기 결정)

  • Lee, Jun-Hyeon;Choe, Sang-U
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.24 no.1 s.173
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    • pp.52-61
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    • 2000
  • The role of quantitative nondestructive evaluation of defects is becoming more important to assure the reliability and the safety of structure, which can eventually be used for residual life evaluation of structure on the basis of fracture mechanics approach. Although ultrasonic technique is one of the most widely used techniques for application of practical field test among the various nondestructive evaluation technique, there are still some problems to be solved in effective extraction and classification of ultrasonic signal from their noisy ultrasonic waveforms. Therefore, crack size determination through a neural network based on the back-propagation algorithm using back-scattered ultrasonic signals is established in this study. For this purpose, aluminum plate containing vertical or inclined surface breaking crack with different crack length was used to receive the back-scattered ultrasonic signals by pulse echo method. Some features extracted from these signals and sizes of cracks were used to train neural network and the neural network's output of the crack size are compared with the true answer.

Acute Back Pain Care after Mandibular Block Anesthesia in an Aged Woman with Multiple sclerosis -A Case Report- (다발성 경화증 노인환자에서 하악 전달마취 시행후 발생된 급성 요통치험 1예 -증례 보고-)

  • Lee, Chun-Ui;Mo, Dong-Yub;Yoo, Jae-Ha;Choi, Byung-Ho;Kim, Jong-Bae
    • Journal of The Korean Dental Society of Anesthesiology
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    • v.10 no.2
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    • pp.197-202
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    • 2010
  • Multiple sclerosis is a degenerative disease prevalent in northern climates, and its cause is unknown. The histopathological lesion in multiple sclerosis is the sclerotic "plague", a discrete focus of myelin loss with maintenance of axon segments and glial proliferation. The plaques may be seen in widely different brain and spinal tissues. The common causes of low back pain are psychosomatic disorder, myofascial pain dysfunction syndrome and herniation of nucleus pulposus. Local anesthetics cross the blood-brain barrier and the signs of CNS toxicity appear at a level between 4.5 and $7.0\;{\mu}g/ml$. This is a case report of acute back pain care after mandibular block anesthesia for the surgical extraction of mandibular root rests in an old aged woman with multiple sclerosis.

Ionic Liquids as Benign Solvents for the Extraction of Aromatics

  • Hossain, Md. Anwar;Lee, Jee-Sun;Kim, Dai-Hyun;Nguyen, Dinh Quan;Cheong, Min-Serk;Kim, Hoon-Sik
    • Bulletin of the Korean Chemical Society
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    • v.33 no.10
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    • pp.3241-3247
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    • 2012
  • Ionic liquids (ILs) have been extensively investigated as promising alternatives to conventional organic solvents such as sulfolane and N,N-dimethylformamide for the selective extraction of aromatic hydrocarbons from the $C_6-C_{10}$ hydrocarbon mixtures produced from the cracking processes of naphtha and light oils. The most important advantage of ILs over conventional organic solvents is that they are immiscible with aliphatic hydrocarbons, and thus the back extraction of ILs from the raffinate phases and top hydrocarbon-rich layers is not necessary. In this paper, a brief review on the state of the art in the utilization of ILs for aromatics separation is presented.