• Proceedings of the Korea Information Processing Society Conference
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• 2023.05a
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• pp.250-252
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• 2023

본 논문에서는 인터넷으로 블록체인 플랫폼 및 서비스를 제공하는 BaaS 환경에서 IAM 서비스를 이용한 개인정보 보호 시스템을 설계했다. 서비스 사용자는 API를 통해 클라우드에 데이터를 요청하는데, 사용자와 클라우드 사이에 API 미들웨어를 두어 클라우드 데이터에 직접 접근하지 못하도록 한다. 또한, 미들웨어의 역할을 하는 서버는 IAM(Identity & Access Management) 서비스에서 생성한 역할 및 정책, 권한에 따라 허가된 데이터에만 접근하며 사용자의 데이터 요청 전/후로 인증 절차를 진행해 시스템의 기밀성 및 무결성을 만족할 수 있도록 한다.

• Microbiology and Biotechnology Letters
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• v.37 no.1
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• pp.55-61
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• 2009

Ginseng saponins (a secondary metabolite, termed ginsenosides) are the principal bioactive ingredients of ginseng, and modification of the sugar chains may markedly change the its biological activity. One of soil bacteria having $\beta$-glucosidase (to transform ginsenoside $Rb_1$) activity was isolated from soil of a ginseng field in Daejeon. 16S rRNA gene sequence analysis revealed that the isolate belonged to the genus Cellulosimicrobium, with highest sequence similarity (99.7%) to Cellulosimicrobium funkei ATCC BAA-$886^T$. The strain, Gsoil 235, could transform ginsenoside $Rb_1$ into Rd, $Rg_3$ and 3 of un-known ginsenosides by the analyses of TLC, HPLC. By investigating its deglycosylation progress, the optimal broth for, $\beta$-glucosidase was nutrient broth (In 48 hours, almost ginsenoside $Rb_1$ could be transformed into minor ginsenosides). On the contrary, the optimal broth for growth was determined as trypic soy broth (TSB).

• Journal of the Korean Society of Clothing and Textiles
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• v.19 no.3
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• pp.516-524
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• 1995

The purpose of this study was to analyze the variation of body shape and to. divide growth period into some age groups based on body type. Duncan Test, Heath-Carter's somatotyping method and allottery equation (y=baa) were applied to semiautomatic data concerning obesity/leakiness. The materials were 309 females aged from 12 to 17 years old. The following results were obtained: 1. In the Developmental trend of the human body, the 12-13 age groups belonged to the acceleration stage of adolescent growth spurt. The 14-15 age groups belonged to the latter period of adolescent growth. The 16-17 age groups belonged to the termination stage of adolescent growth. 2. In the Heath-Carter's somatotyping method, the average somatotype changed into M.M- C-C-M-D with age. 3. In the alphamerical analysis, the first critical point appeared at the stature 146 Cm in case of weight, LBM and fat. The second critical point appeared at the stature 154-157 Cm in case of LBM and Fat. All the three measurement showed positive allotmentty.

• Journal of Plant Biotechnology
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• v.33 no.1
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• pp.27-32
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• 2006

To clarify the roles of bromelain in plants, we isolated BL1 gene encoding bromelain from pineapple stem tissues and sequenced. The full length cDNA is 933 bp and encodes a polypeptide of 311 amino acid residues. The cDNA is most similar 94% at the amino acid level to bromelain previously isolated from pineapple (BAA21929). Explants of Lactuca sativa were co-cultivated with Agrobacterium tume-faciences LBA 4404 strains containing nptII and BL1 gene for transformation. Through initial selection of regenerated explants by culturing on a kanamycin and carbenicillin containing MS medium, multiple shoots were obtained after 2 months of culture. For a complementary step of selection, putative transgenic shoots were transferred to 1/2 Ms basal medium supplemented with 100 mg/L kanamycin and 500 mg/L carbenicillin. The selected shoots were obtained T1 generation seeds with emasculation, and tested with PCR analysis using 35S promoter and BL1 specific primers whether BL1 gene was introduced to genome of the plants. These results confirmed that produced the specific PCR bands in the putative transgenic lines. Additionally the Northern blot and endo protease activity showed that transcripts of BL1 gene were detected in transgenic lines. Theses results suggest that BL1 gene be successfully integrated and transcripted in the transgenic lettuce plants.

• Journal of Korean Society of Archives and Records Management
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• v.19 no.3
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• pp.223-245
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• 2019

As the foundation for the Fourth Industrial Revolution, blockchain is becoming an essential core infrastructure and technology that creates new growth engines in various industries and is rapidly spreading to the environment of businesses and institutions worldwide. In this study, the characteristics and trends of blockchain technology were investigated and arranged, its application to the records management section of public institutions was required, and the procedures and methods of construction in the records management field of public institutions were studied in literature. Finally, blockchain technology was applied to the records management to propose an archive chain model and describe possible expectations. When the transactions that record the records management process of electronic documents are loaded into the blockchain, all the step information can be checked at once in the activity of processing the records management standard tasks that were fragmentarily nonlinked. If a blockchain function is installed in the electronic records management system, the person who produces the document by acquiring and registering the document enters the metadata and information, as well as stores and classifies all contents. This would simplify the process of reporting the production status and provide real-time information through the original text information disclosure service. Archivechain is a model that applies a cloud infrastructure as a backend as a service (BaaS) by applying a hyperledger platform based on the assumption that an electronic document production system and a records management system are integrated. Creating a smart, electronic system of the records management is the solution to bringing scattered information together by placing all life cycles of public records management in a blockchain.

• Journal of the Korean Society of Food Science and Nutrition
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• v.42 no.9
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• pp.1439-1445
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• 2013

The aim of this study was to investigate the quality characteristics of rice cookies specifically prepared with rice flour following enzyme treatment. In this study, three types of specific enzyme treatments, that is, ${\alpha}$-amylase, ${\beta}$-amylase, and cellulose+${\beta}$-glucanase were applied on the effect of rice flour with respect to desired quality characteristics of the rice cookies. Based on our study results, we have found that the density of dough in the rice cookies was not significantly different between that of the non-enzyme treated (control) and the enzyme treated group. However, we found that the spread factor of rice cookies, prepared with rice flour treated with ${\beta}$-amylase, was higher than that of the control. It was also found that the moisture content of rice cookies (with added enzyme-treated rice flour) was 3.20 to 3.90%; however, this range is much lower than that observed in the control. Further, we observed that Hunter color's L-values were significantly higher for the control than those of the enzyme-treated cookies. We also found that hardness of enzyme-treated cookies was comparatively better than that of the control. In addition, the sensory acceptability scores of the enzyme-treated cookies were found to be significantly higher than the control in decisive parameters such as aroma, appearance, flavor, taste, texture, and overall acceptability. Based on our findings, we suggest that ${\alpha}$-amylase treated rice flour is an effective ingredient for improving the overall quality of rice cookies.

• Journal of Microbiology and Biotechnology
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• v.16 no.12
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• pp.1862-1867
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• 2006

A moderately halophilic, Gram-positive, spore-forming bacterium was isolated from the roots of Blutaparon portulacoides, a plant found in sandy soil parallel to the beach line in Restinga de Jurubatiba, Rio de Janeiro, Brazil. The strain, designated $M9^T$, was motile and strictly aerobic with rod-shaped cells. It grew in the absence of NaCl and up to 20% NaCl, and was able to hydrolyze casein and starch. Strain $M9^T$ had a cell-wall peptidoglycan based on L-Orn-D-Asp, the predominant menaquinone present was menaquinone-7 (MK-7), diaminopimelic acid was not found, and anteiso-$C_{15:0}$ and iso-$C_{15:0}$ were the major fatty acids. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain $M9^T$ belonged to the genus Halobacillus and exhibited 16S rRNA gene similarity levels of 97.8-99.4% with the type strains of the other nine Halobacillus species. The DNA-DNA relatedness of strain $M9^T$ with H. trueperi, the closest relative as regards 16S rRNA gene similarity, and H. locisalis was 21% and 18%, respectively. Therefore, on the basis of phenotypic, genotypic, and phylogenetic data, strain $M9^T$ (=ATCC BAA-$1217^T$, =CIP $108771^T$, =KCTC $3980^T$) should be placed in the genus Halobacillus as a member of a novel species, for which the name Halobacillus blutaparonensis sp. nov. is proposed.

• Journal of the Korean Society of Food Science and Nutrition
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• v.8 no.1
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• pp.25-30
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• 1979

A bacterial strain which showed a remarkable tolerance against cadmium was isolated from waste water and identified as a member of Staphylococcus aureus. Heavy metal ions, at the concentration of 10 ppm and other than cadmium, inhibited at least by 30 per cent of turbidometric growth of the organism. The organism exhibited a normal pattern of growth with increasing concentration of cadmium up to 50 ppm. However, they were unable to grow in the concomitant presence of cadmium higher than 500 ppm. In spite of the drastic growth inhibition of cadmium, the organism was able to grow even in the concomitant presence of 500 ppm cadmium when it was previously cultivated for 15 hours with exposing to 10 ppm cadmium or lead.

• Journal of Venture Innovation
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• v.2 no.1
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• pp.13-21
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• 2019

In LTE environment, which is 4th generation mobile communication systems, there is concern about private information exposure by transmitting initial identifier in plain text. This paper suggest mutual authentication protocol, which uses one-time password utilizing challenge-response and AES-based Milenage key generation algorithm, as solution for safe initial identification communication, preventing unique identification information leaking. Milenage key generation algorithm has been used in LTE Security protocol for generating Cipher key, Integrity key, Message Authentication Code. Performance analysis evaluates the suitability of LTE Security protocol and LTE network by comparing LTE Security protocol with proposed protocol about algorithm operation count and Latency.Thus, this paper figures out initial identification communication's weak points of currently used LTE security protocol and complements in accordance with traditional protocol. So, it can be applied for traditional LTE communication on account of providing additional confidentiality to initial identifier.

• Journal of Microbiology and Biotechnology
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• v.26 no.6
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• pp.1026-1034
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• 2016

In the current study, a total of 135 enterococci strains from different sources were screened for the presence of the enterocin-encoding genes entA, entP, entB, entL50A, and entL50B. The enterocin genes were present at different frequencies, with entA occurring the most frequently, followed by entP and entB; entL50A and L50B were not detected. The occurrence of single enterocin genes was higher than the occurrence of multiple enterocin gene combinations. The 80 isolates that harbor at least one enterocin-encoding gene (denoted "Gene⁺ strains") were screened for antimicrobial activity. A total of 82.5% of the Gene⁺ strains inhibited at least one of the indicator strains, and the isolates harboring multiple enterocin-encoding genes inhibited a larger number of indicator strains than isolates harboring a single gene. The indicator strains that exhibited growth inhibition included Listeria innocua strain CLIP 12612 (ATCC BAA-680), Listeria monocytogenes strain CDC 4555, Enterococcus faecalis ATCC 29212, Staphylococcus aureus ATCC 25923, S. aureus ATCC 29213, S. aureus ATCC 6538, Salmonella enteritidis ATCC 13076, Salmonella typhimurium strain UK-1 (ATCC 68169), and Escherichia coli BAC 49LT ETEC. Inhibition due to either bacteriophage lysis or cytolysin activity was excluded. The growth inhibition of antilisterial Gene⁺ strains was further tested under different culture conditions. Among the culture media formulations, the MRS agar medium supplemented with 2% (w/v) yeast extract was the best solidified medium for enterocin production. Our findings extend the current knowledge of enterocin-producing enterococci, which may have potential applications as biopreservatives in the food industry due to their capability of controlling food spoilage pathogens.