• Journal of Microbiology
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• v.44 no.3
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• pp.311-319
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• 2006

Bud6p is a component of a polarisome that controls cell polarity in Saccharomyces cerevisiae. In this study, we investigated the role of the Candide albicans Bud6 protein (CaBud6p) in cell polarity and hyphal development. CaBud6p, which consists of 703 amino acids, had 37% amino-acid sequence identity with the Bud6 protein of S. cerevisiae. The homozygous knock-out of CaBUD6 resulted in several abnormal phenotypes, such as a round and enlarged cells, widened bud necks, and a random budding pattern. In hypha-inducing media, the mutant cells had markedly swollen tips and a reduced ability to switch from yeast to hypha. In addition, a yeast two-Hybrid analysis showed a physical interaction between CaBud6p and CaAct1p, which suggests that CaBud6p may be involved in actin cable organization, like Bud6p in S. cerevisiae. Taken together, these results indicate that CaBud6 plays an important role in the polarized growth of C. albicans.

• Horticultural Science & Technology
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• v.19 no.4
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• pp.535-539
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• 2001

To establish the cultural practices of persimmon in heated plastic house, the growth and flower bud differentiation of 4-year old 'Nishimurawase' persimmon tree planted in 80 L pots were investigated. Temperature was kept at above $18^{\circ}C$. The earlier heating induced the earlier flower bud differentiation, while the number of days from bud burst to flower bud differentiation was 70-75 days, which was not affected by the date of heating. The date of flower bud differentiation in 'Nishimurawase' persimmon was May 1, May 21, and June 1 in the plastic house heated from Jan. 15, Feb. 15, and Mar. 15, respectively. The number of flower primordia per bud in the plastic house was 6.2-7.1, slightly fewer than that of 7.8 in the open-field, with the tendency of increasing the number by earlier heating. The days required for the differentiation of the last flower bud primordia from the beginning of differentiation of the first bud were 40-60, and that required for the flower bud differentiation of all buds on the same shoot was 30. The total carbohydrates in shoot increased and the contents of nitrogen decreased, resulting in increased C/N ratio just before flower bud differentiation, and it coincided with the time when the total carbohydates increased above 15%. During two weeks before flower bud differentiaton, The contents of zeatin per 1 g dry matter of shoot drastically increased from $27.2{\mu}g$ to $47.3{\mu}g$, while that of IAA slightly decreased from $188.6{\mu}g$ to $172.4{\mu}g$, and that of GA decreased from $2,225{\mu}g$ to $1,555{\mu}g$.

• Journal of Ginseng Research
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• v.6 no.1
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• pp.67-74
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• 1982

To clarify the morphological characteristics of Korean ginseng cultivated, the shapes and frequencies of Inflorescence, flowering bud, fruit, and leaf were investigated. The shapes of inflorescence, flowering bud and fruit, and leaf were divided into 6, 3, and 7 groups, respectively. The frequencies of these shapes were not significantly different according to the planting positions.

• Journal of Sericultural and Entomological Science
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• v.37 no.1
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• pp.6-9
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• 1995

To establish a regeneration system of mulberry through the adventitious bud formation of winter bud leaflet, medium conditions were examined. Adventitious bud formation rate of mulberry winter bud leaflet on the medium containing different concentrations of BA and TDZ as the growth regulators was high on 5mM BA and 0.5mM TDZ. The effect of preculture on the medium containing BA or TDZ was affected not only by the concentration of growth regulators but the duration of preculture. Fructose as carbon source was effective on adventitious bud formation.

• Journal of Sericultural and Entomological Science
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• v.16 no.1
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• pp.1-20
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• 1974

These studies are conducted to find out inducing factors for the non-sprouting bud which appears in the utility reclaimed hill mulberry held and to establish control method for it since 1971. The results are as follows： 1. In spring, winter bud does not germinate at all on the top of branches or does wither suddenly after or during its germination. Necrosis and browning are appeared in cortex and phloem of non-sprouting bud branch. In autumn, the deterioration of leaves, the abnormal leaves on the top of branch, and browning of veins or petioles are also observed. 2. The soil of non-sprouting bud mulberry Held were found to be sand loom derived from granite rocks. The sub soil of it was very poor in development of root system because it had hard soil and lower pore space ratio. Especially, the sub soil to bring about severe non-sprouting bud had been easily appeared with deficiency of moisture content because of high density solid. 3. Content of soil moisture was significantly lower in the severe non-sprouting bud soil than in the healthy field. The sub soil o( the healthy field contained proper moisture content. On the other hand, the sub soil of the severe non-sprouting bud held contained almost critical moisture content for wilting. 4. The depth of available soil was shallow in the non-sprouting bud than in the healthy fold. The more rate of the non-sprouting bud was severe, the more available soil depth was sallow. 5. Available boron content in soil was affected by moisture content in soil. There was. lower moisture content in the non-sprouting bud field than that in healthy fold during 5, June to 5, September. 6. There was no significant correlation between soil pH and available boron content. On the other hand, the correlation of the content of organic matter and available boron content appeared to be highly positive significance. 7. The quantity of boron was significantly contained more in healthy mulberry field (0.34∼0.43ppm) than in non-sprouting bud field (0.10∼0.28ppm). 8. Boron content in leaves and barks was significantly lower in the non-sprouting bud trees than in healthy trees. 9. The symptom of non-sprouting bud induced from boron free sand culture was similar with that arised in the non-sprouting bud field. 10. The rate of non-sprouting bud was high by the increased application of lime. Considering the facts mentioned the above, author may conclude that the non-sprouting bud of mulberry tree is caused by boron deficiency, but also it is affected by the parent rocks, organic matter, soil moisture content and lime application. 11. The non$.$sprouting bud may be completely controlled by the application of 6∼9kg borax per 10a mulberry field twice a year in spring and summer.

• Journal of Ginseng Research
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• v.4 no.1
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• pp.65-71
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• 1980

Twin-bud seedlings and four-leaflet seedlings of ginseng were found and transplanted to field and their growth characteristics were investigated. 1. Appearance frequencies of twin-bud and four-leaflet seedlings were 0.3 and 0.4 percent, respectively, in common nursery bed: and were 6.6 and 28.4 percent, respectively, in polystem line. 2. Generally, the growth of twin.bud and four-leaflet seedlings were better than those of common seedlings both in aerial part and in root. Root weights of both type seedlings exceeded the common ones by 66 and 38 percent, respectively. 3. When they became two-year-old plants, leafiet number of common plant was 11.6, and those of twin-bud and four-leaflet-seedling plants were 18.1 and 13.8, respectively. There were no inflorescence in twin-bud-seedling plant, but the ratios of in florescent Plant in four-leaflet.seedling and common plant were 44.0 and 12.5 percent, respectively. 4. In two-year-old plant, root weights of twin-bud and four-leaflet-seedling plants were heavier than those of common ones by 27 and 20 percent, respectively.

• Journal of Bio-Environment Control
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• v.20 no.4
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• pp.382-386
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• 2011

This study aims to identify the cause for low appearance of flowers in 'Heukgoosul' grapevines, and determine basic characteristics of the axillary buds and types of necrosis in order to find out their influence on necrosis. As for $1^{st}$ to $4^{th}$ node, the closer buds were located to cane, the smaller the size of buds became. However, there was no difference in the size of an axillary bud in the upper part of the shoot above the 4th node. The bud necrosis occurrence in the $1^{st}$ node was the highest with 32% while buds in $4^{th}$ to $10^{th}$ node were normal with 84~96%. The size of buds in July was the largest with 6.40 mm, while buds showed no difference from August to October. The bud necrosis and main bud necrosis occurred most frequently in October, however accessory bud necrosis took place from July demonstrating no difference since then. The analysis on the relationship between shoot vigor and necrosis of axillary buds showed that the shoot diameter and internodes' length have no co-relationship with axillary bud necrosis, but there were negative relationship between the size of buds and necrosis occurrence, which was the most related to accessory bud necrosis. Therefore, despite the low occurrence of bud necrosis and healthy buds in 'Heukgoosul' grapevines, there was little appearance of flowers in the grapevines, which was attributable to the necrosis in the first bud. In conclusion, the research suggests spur pruning with three nodes kept intact yield.

• Korean Journal of Plant Resources
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• v.8 no.3
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• pp.265-274
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• 1995

Characteristics of vegetative spur leaf, spur bud, fruiting supr leaf and flowering in relation to canopy position was investigated on order to promote better spur bud and flower bud differentiation on Citrus junos. There was no difference in vegetative spur leaf characteristics among directions. Vegetative spur leaf number and leaf area showed no difference between top and bottom position. There was no difference in spur bud size among directions, but spur bud at top was larger than that at bottom. Specific leaf weight of vegetative spur was mostly influenced by light interception, and leaf dry weight per spur average leaf dry weight, spur bud length and diameter had also a very high correlation with light interception rate. Shading and GA treatment in spur bud and flower bud differentiation was ineffective than natural light. Fruit thinning enhanced fruit quality as well as flower bud differentiation through an increase of leaf number per fruit, over 40 in Citrus junos.

• International Journal of Oral Biology
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• v.33 no.3
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• pp.83-89
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• 2008

Glutamate-induced cobalt uptake reveals non-NMDA glutamate receptors (GluRs) in rat taste bud cells. Previous studies suggest that glutamate-induced cobalt uptake in taste cells occurs mainly via kainate type GluRs. Cobaltstained cells were immunoreactive against GluR6 and KA1 subunits of GluRs. However, the functions of those type of receptors are not known yet. It is important question which types of taste cells are cobalt-stained when stimulated by glutamate and whether they express these kinds of GluRs. Circumvallate and foliate papilla of Sprague-Dawley rats (45-60 days old) were used. A cobalt-staining technique combined with immunohistochemistry against specific markers for taste bud cell types, such as blood group H antigen (BGH), $\alpha$-gustducin (Gus), or neural cell adhesion molecule (NCAM) was employed. We also performed double labeling of GluR6 or KA1 subunits of GluR with each specific marker for taste bud cell types. Lots of cobaltstained taste bud cells expressed Gus-like immunoreactivity, and subsets of the cobalt stained cells appeared NCAM- or BGH-like immunoreactivity. Stimulation with 1 mM glutamate significantly increased the number of cobaltstained cells in Gus-like immunoreactive cells, but not in NCAM- or BGH-like immunoreactive cells. In the double labeling experiments, GluR6 and KA1 subunits of GluRs were mainly expressed with Gus. These results suggest that kainate glutamate receptors preferentially expressed in type II taste bud cells in rat.

• Korean Journal of Plant Resources
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• v.15 no.1
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• pp.8-17
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• 2002

To improve new propagation method, I try to propagate wild polygonatum odoratum roots which formate adventitious bud without bud. The obtained results of size of roots and adventitious bud to formate for multiple propagation of wild polygonatum odoratum as the follow. One year grown wild polygonatum odoratum root in 5cm diameter formate 66.5％ adventitious buds. One year grown wild polygonatum odoratum root in 15cm in diameter germinate 113% adventitious bud. Two years grown root in 15cm diameter formate adventitious bud three times more then one year root. Wild polygonatum odoratum in 15cm diameter without any bud in experiment plot makes 4 times of propagation. Sand and Perlite media propagaton plot was increased 3.1∼3.3times more than common culture medium. The best result of mass propagation wild polygonatum odoratum is 10mm diameter root and 5cm cut in length to plant sand media plot.