• Korean Journal of Environment and Ecology
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• v.27 no.6
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• pp.666-675
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• 2013

This research was carried out from March 2009 to July 2012 to explicate the height change based breeding-ecological characteristics of Titmice that were breeding in the artificial nests installed in the Halla Mountain. The first egg-laying day was delayed as the height of the area under investigation increased, and it turned out that the decrease in temperature due to the elevated height had influence on that delay (p<0.05). Generalizing the annual results of the analyses, we see that there was no significant correlation occurring between the average temperatures and egg-laying days in the breeding season (April~June) (p>0.05). The rate of artificial nest use was not different among areas and among year, but between the species, Parus major and Parus varius. There were differences in clutch sizes among species (p>0.05), but no differences in heights and years (p<0.05). The hatching-, fledging-, and breeding-success rates were 67.3%, 99.5%, and 67.0% respectively in 2011, and 71.3%, 96.8%, 69.0% respectively in 2012, from which we can tell that the fledging-success rate was high but the hatching- and breeding-success rates were similar. Though we confirm that temperature changes due to height differences had influence on breeding periods, but we cannot identify a significant correlation with other breeding-ecological characteristics. Thus, in order to more precisely explicate the influence of temperature changes due to heights on Titmice's breeding, long-term research is needed in terms of the investigation of microclimatic factors and food resource.

• Korean Journal of Plant Resources
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• v.28 no.5
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• pp.648-655
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• 2015

Balloon flower (Platycodon grandiflorum A. DC.) is a perennial plant of mainly Campanulaceae family, which have been widely used as a food ingredient and herbal medicine in East Asia. Although demands on related products and yearly cultivation area for balloon flower are increasing, diverse fundamental technologies and molecular breeding studies are not very well supported in Platycodons. In this study, 30 random amplification of polymorphic DNA (RAPD) primers were test in an attempt to explore genetic diversities. In addition, sequences information of the actin gene, a well conserved gene encoding a globular protein that forms microfilaments, was retrieved and analyzed. Two actin homologs were recovered; 3.4 kb fragment is a Pg-actin and 1.4 kb fragment is a Pg-actin homolog with 28.6% similarity. We have confirmed that the Pg-actin gene is configured into 4 exons and 3 introns. A single nucleotide polymorphism (SNP), G↔A, was detected on the intron 3, which served as a target for the CAPS marker development. The marker Pg-Actin-Int3 was applied to 32 balloon flower accessions. Balloon flower DNA sequence information generated in this study is expected to contribute to the analysis and molecular breeding and genetic diversity analysis of balloon flowers.

• Korean Journal of Breeding Science
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• v.49 no.2
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• pp.80-86
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• 2017

'Jungmo2501' (Avena sativa L.), a winter oat for forage use, was developed by the breeding team at the National Institute of Crop Science, RDA in 2010. The following is the characteristics of 'Jungmo2501' that is characterized as light green leaf, yellow brown culm and whitish yellow grain. The heading date of 'Jungmo2501' was about 3 days earlier than that of check cultivar 'Samhan'(May 7 and May 10, respectively). Its plant height was 11 cm longer than 103 cm of the check, and the leaf blade ratio of aerial parts was 26 % higher than the check (11.8% and 9.4%, respectively). The cold tolerance, resistance to lodging and wet injury of 'Jungmo2501' were similar to those of the check. The average forage dry matter yield of 'Jungmo2501' harvested at milk-ripe stage was 5% higher than the check ($15.5ton\;ha^{-1}$ and $14.7ton\;ha^{-1}$, respectively). 'Jungmo2501' was higher than the check in terms of protein content (6.6% and 5.9%, respectively), neutral detergent fiber (58.5% and 57.6%, respectively), and acid detergent fiber (34.5% and 32.1%, respectively), while total digestible nutrients was lower than the check (61.6% and 63.6%, respectively), and TDN yield was $0.37ton\;ha^{-1}$ more than that of the check ($9.71ton\;ha^{-1}$ and $9.34ton\;ha^{-1}$, respectively). The silage grade of 'Jungmo2501' estimated by Flig score showed level II, meaning good quality. Fall sowing cropping of 'Jungmo2501' is recommended only for areas where average daily minimum mean temperatures in January are higher than $-6^{\circ}C$.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.108-108
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• 2017

The objective of this study was to know prospect and present state of new variety breeding of Forage and Grassland in South Korea. The industry of forage in Korea is going up to expend through utilization of good quality forage, Italian ryegrass, forage corn and oat rather than rice straw. Especially, since 2007, Italian ryegrass(IRG) had been very important major winter forage crop in South Korea and developed 13 varieties including very early maturity variety (three varieties), early maturity variety (three varieties), medium maturity variety (one variety) and late maturity variety (six varieties). But the disadvantage of Italian ryegrass was weak winter hardness and drought but has good advantage of forage nutritive value, high-yielding and high sugar content, and like livestock as like hanwoo, dairy cattle, goat so on. The Ko-variety (Korea developed variety) of Italian ryegrass has high cold-tolerant and adaptability more than any other country developed variety, and expend to cultivation area from southern area (below Daejeon) to middle-northern area (upper Han river). Although the cultivation area of Italian ryegrass of South Korea was 21,700 ha in 2007, right now, that of Italian ryegrass is about 123,600ha due to expend cultivation area and know famer to good forage crop and have a various maturity varieties (very early, early, medium, late) according to local situation (before-crop harvesting stage or double cropping system). The seed market of Italian ryegrass in South Korea becoming extended to around 100 million Korean won and seed export get nearer to foreign country. We are going to develop of new variety for stress tolerant and high yield and quality forage variety, good adaptability to the Korean environmental conditions including reclaimed area, make self-sufficiency system for forage seed (Italian ryegrass), export our seed to foreign countries.

• Journal of The Korean Society of Grassland and Forage Science
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• v.35 no.1
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• pp.57-62
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• 2015

"Nokyang" (Hordeum vulgare L.), a new whole-crop silage barley cultivar, was developed by the breeding team at the Department of Rice and Winter Cereal Crop, National Institute of Crop Science, RDA, Korea in 2012. It was derived from combining "Nagyoung" and breeding line "SB77368-B-145". Among the combinations made in 2001, a promising line, "SB01T2017", showed good characteristics in terms of potential forage yield in the yield trial at Iksan from 2007 to 2009. In 2009, the promising line was designated as "Iksan457" and placed in regional yield trials at six locations around Korea for three years from 2010 to 2012, and was released under the name of "Nokyang." It has the growth habit of a group III creeping plant type, with a light-green leaf and common spike. Its average heading and maturing dates were on May 6 and June 4, respectively, which were later than cultivar "Youngyang." "Nokyang" also showed strong winter hardiness, and a resistance to shattering and barley yellow mosaic virus (BaYMV) similar to those of the check cultivar. The best thing among the traits of one is a new good quality with the plant green at the latter growing period. The average forage dry matter (DM) yields in the regional yield trial were about 12.8 and 11.5 MT $ha^{-1}$ in upland and paddy field, respectively, which were higher than those of the check cultivar, by 7% and 2%, respectively. This cultivar would be suitable for an area of the Korean peninsula where the daily minimum temperature in January is above $-8^{\circ}C$.

• Korean Journal of Agricultural Science
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• v.48 no.4
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• pp.681-691
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• 2021

The estimated breeding value (EBV) and accuracy of Hanwoo steer (Korean cattle) is an indicator that can predict the slaughter time in the future and carcass performance outcomes. Recently, studies using pedigrees and genotypes are being actively conducted to improve the accuracy of the EBV. In this study, the pedigree and genotype of 46 steers obtained from livestock farm A in Gyeongnam were used for a pedigree best linear unbiased prediction (PBLUP) and a genomic best linear unbiased prediction (GBLUP) to estimate and analyze the breeding value and accuracy of the carcass weight (CWT), eye muscle area (EMA), back-fat thickness (BFT), and marbling score (MS). PBLUP estimated the EBV and accuracy by constructing a numeric relationship matrix (NRM) from the 46 steers and reference population I (545,483 heads) with the pedigree and phenotype. GBLUP estimated genomic EBV (GEBV) and accuracy by constructing a genomic relationship matrix (GRM) from the 46 steers and reference population II (16,972 heads) with the genotype and phenotype. As a result, in the order of CWT, EMA, BFT, and MS, the accuracy levels of PBLUP were 0.531, 0.519, 0.524 and 0.530, while the accuracy outcomes of GBLUP were 0.799, 0.779, 0.768, and 0.810. The accuracy estimated by GBLUP was 50.1 - 53.1% higher than that estimated by PBLUP. GEBV estimated with the genotype is expected to show higher accuracy than the EBV calculated using only the pedigree and is thus expected to be used as basic data for genomic selection in the future.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.6
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• pp.779-783
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• 2006

The adiponectin gene is known to be involved in the regulation of energy homeostasis involving food intake, carbohydrate and lipid metabolism. Human adiponectin gene polymorphisms have been recently reported to be associated with obesity, insulin sensitivity and the risk of type 2 diabetes. The present study was carried out to investigate the porcine adiponectin gene as a candidate gene for fat deposition and carcass traits. A mutation of A178G of the porcine adiponectin gene that resulted in substitution of the amino acid Isoleucine to Valine was identified. AcyI PCR-RFLP was used to detect the polymorphism of the genotypes in five different pig populations (Large White, Landrace, Duroc, Chinese breeds Meishan and Qingping). The A allele frequency was significantly higher among subjects from Chinsese lard type breeds, while the G allele was the only one present in those from Western lean type breeds. To determine if there was an association of the polymorphism with phenotypic variation, the mutation was tested in 267 pigs of the "Large $White{\times}Meishan$" F2 resource population. The results of association analyses showed significant associations of the genotypes with fat deposition and carcass traits. Allele G was significantly associated with increase in loin eye height, loin eye area and lean meat percentage and bone percentage, and decrease in fat mean percentage, ratio of lean to fat, shoulder fat thickness, 6-7 rib fat thickness, thorax-waist fat thickness and buttock fat thickness. The substitution of A178G (Ile60Val) happened to be located at amino acid 60 in the collagenous domain of porcine adiponectin which might affect the association into higher-order structures, and accordingly affect the posttranslational modifications and optimal biological activity of the multimeric forms. The identified functional polymorphism provides new evidence of adiponectin as an important candidate gene affecting fat deposition and carcass traits in pigs.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.3
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• pp.324-328
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• 2006

Carbonic anhydrase III (CA3) is a member of a multigene family that encode carbonic anhydrase isozymes. In this study, a complete coding sequence of the pig CA3 gene which encodes a 260 amino-acid protein was determined. The amino acid comparison showed high sequence similarities with previously identified human (86.5%) CA3 gene and mouse (91.5%) Car3 gene. The partial genomic DNA sequences were also investigated. The length of intron 1 was 727 bp. Comparative sequencing of three pig breeds revealed that there was a T${\rightarrow}$C substitution at position 363 within intron 1. The substitution was situated within a NcoI recognition site and was developed as a PCR-restriction fragment length polymorphism (RFLP) marker for further use in population variation investigations and association analysis. Two alleles (A and B) were identified, and 617 bp fragments were observed for the AA genotype and 236 bp and 381 bp fragments for the BB genotype. The polymorphism of CA3 was detected in 8 pig breeds. Allele B was predominant in the Western pig breeds. In addition, association studies of the CA3 polymorphism with carcass traits in 140 $Yorkshire{\times}Meishan$ $F_2$ offspring showed that the NcoI PCR- RFLP genotype may be associated with variation in several carcass traits of interest for pig breeding. Allele B was associated with increases in lean meat percentage, loin eye height and loin eye area. Statistically significant association with backfat thickness was also found; pigs with the AB genotype had much less backfat thickness than AA or BB genotypes.

• Journal of Korean Society of Forest Science
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• v.90 no.1
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• pp.36-42
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• 2001

Changes in breeding bird community and forest structure by thinning were investigated from April 1997 to August 1998 in 10ha ($400{\times}250m$) of temperate mixed hardwood forest in Mt. Gariwang which is located in National Forest, Pyoungchang, Kangwon Province, Korea. Bird community was surveyed by the territory mapping method and forest structure was done by analyzing the vertical structure of foliage, distribution of diameter at breast height (DBH) of trees and tree species composition. After the thinning, tree density, number of tree species and basal area were decreased from 195ea/ha, 18 species and 6.69 $m^2/ha$ into 100ea/ha, 13 species and 3.04 $m^2/ha$, respectively. Foliage coverages in upper and mid layer were decreased, but coverage in low layer was increased. Large trees (Over 40cm of DBH) were decreased. Number of breeding bird species and pairs were decreased from 14 species and 23 pairs into 8 species and 12 pairs after the thinning. Number of breeding pairs of Yellow-breasted bunting Emberiza elegans incresed. There were decrease in number of species in hole & bush-nesting and canopy & bush-foraging guild. The results suggested that the thinning affect the species composition of breeding bird community and the forest structure. Changes of habitat structure might influence in breeding bird's density, species diversity and habitat using pattern of breeding bird community.

• Journal of Animal Science and Technology
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• v.65 no.4
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• pp.720-734
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• 2023

In Korea, Korea Proven Bulls (KPN) program has been well-developed. Breeding and evaluation of cows are also an essential factor to increase earnings and genetic gain. This study aimed to evaluate the accuracy of cow breeding value by using three methods (pedigree index [PI], pedigree-based best linear unbiased prediction [PBLUP], and genomic-BLUP [GBLUP]). The reference population (n = 16,971) was used to estimate breeding values for 481 females as a test population. The accuracy of GBLUP was 0.63, 0.66, 0.62 and 0.63 for carcass weight (CWT), eye muscle area (EMA), back-fat thickness (BFT), and marbling score (MS), respectively. As for the PBLUP method, accuracy of prediction was 0.43 for CWT, 0.45 for EMA, 0.43 for MS, and 0.44 for BFT. Accuracy of PI method was the lowest (0.28 to 0.29 for carcass traits). The increase by approximate 20% in accuracy of GBLUP method than other methods could be because genomic information may explain Mendelian sampling error that pedigree information cannot detect. Bias can cause reducing accuracy of estimated breeding value (EBV) for selected animals. Regression coefficient between true breeding value (TBV) and GBLUP EBV, PBLUP EBV, and PI EBV were 0.78, 0.625, and 0.35, respectively for CWT. This showed that genomic EBV (GEBV) is less biased than PBLUP and PI EBV in this study. In addition, number of effective chromosome segments (M_e) statistic that indicates the independent loci is one of the important factors affecting the accuracy of BLUP. The correlation between M_e and the accuracy of GBLUP is related to the genetic relationship between reference and test population. The correlations between M_e and accuracy were -0.74 in CWT, -0.75 in EMA, -0.73 in MS, and -0.75 in BF, which were strongly negative. These results proved that the estimation of genetic ability using genomic data is the most effective, and the smaller the M_e, the higher the accuracy of EBV.