• Journal of Microbiology and Biotechnology
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• v.26 no.11
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• pp.1983-1992
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• 2016

Pseudomonas strains isolated from the heavily contaminated Lubin copper mine and Zelazny Most post-flotation waste reservoir in Poland were screened for the presence of integrons. This analysis revealed that two strains carried homologous DNA regions composed of a gene encoding a DNA_BRE_C domain-containing tyrosine recombinase (with no significant sequence similarity to other integrases of integrons) plus a three-component array of putative integron gene cassettes. The predicted gene cassettes encode three putative polypeptides with homology to (i) transmembrane proteins, (ii) GCN5 family acetyltransferases, and (iii) hypothetical proteins of unknown function (homologous proteins are encoded by the gene cassettes of several class 1 integrons). Comparative sequence analyses identified three structural variants of these novel integron-like elements within the sequenced bacterial genomes. Analysis of their distribution revealed that they are found exclusively in strains of the genus Pseudomonas.

• Preventive Nutrition and Food Science
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• v.19 no.4
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• pp.348-352
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• 2014

Black rice contains many biologically active compounds. The aim of this study was to investigate the protective effects of black rice extracts (whole grain extract, WGE and rice bran extract, RBE) on tert-butyl hydroperoxide (TBHP)-induced oxidative injury in HepG2 cells. Cellular reactive oxygen species (ROS), antioxidant enzyme activities, malondialdehyde (MDA) and glutathione (GSH) concentrations were evaluated as biomarkers of cellular oxidative status. Cells pretreated with 50 and $100{\mu}g/mL$ of WGE or RBE were more resistant to oxidative stress in a dose-dependent manner. The highest WGE and BRE concentrations enhanced GSH concentrations and modulated antioxidant enzyme activities (glutathione reductase, glutathione-S-transferase, catalase, and superoxide dismutase) compared to TBHP-treated cells. Cells treated with RBE showed higher protective effect compared to cells treated with WGE against oxidative insult. Black rice extracts attenuated oxidative insult by inhibiting cellular ROS and MDA increase and by modulating antioxidant enzyme activities in HepG2 cells.

• Proceedings of the Korea Inteligent Information System Society Conference
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• 2007.11a
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• pp.362-366
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• 2007

최근 기업 경영활동의 의사결정시 사용되는 비즈니스 룰(Business Rule)을 정형화하고 하나의 시스템으로 구축하여 효과적으로 기업의 경쟁력을 제고하기 위한 노력이 다양하게 시도되고 있다. 비즈니스 룰 시스템 구축 작업의 경우 기업 내부에 비정형적으로 존재하는 비즈니스 룰을 체계적으로 관리하기 위해 BRMS(Business Rule Management System)와 같은 전문 관리 도구를 도입하나, 대부분의 경우 비즈니스 룰 리포지토리(Repository)를 단순히 기능별 혹은 업무별로 구성함으로 인해 동일한 내용의 룰이 서로 다른 룰 리포지토리에 중복 존재하게 되는 등 구조상의 문제점을 발생시킨다. 이로 인해 각 어플리케이션 간의 룰 또는 룰 세트(Rule Set) 공유 관계가 수동 관리되거나 중복 룰의 수정으로 인한 룰 세트별 버전 관리 문제 등 비즈니스 룰 리포지토리 운영의 어려움에 봉착하게 된다. 본 연구에서는 금융보험사의 룰웨어하우스 구축 사례를 통해 다양한 어플리케이션에서 참조되는 전사 수준의 비즈니스 룰 관리 아키텍처 구성 방법 및 각 방법이 지닌 장단점에 대해 분석한다. 본 연구의 결과를 토대로 다양한 어플리케이션에서 참조되고 수시로 변경되는 전사 수준의 통합 비즈니스 룰 관리 시스템 구축 방안에 대한 연구가 활성화되기를 기대한다.

• Journal of the Architectural Institute of Korea Planning & Design
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• v.34 no.9
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• pp.67-76
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• 2018

The improvements of G-SEED standards was studies in this research by comparing the composition, rating scores, and standards, including categories, issues and criteria of BREEAM in the UK. In this research, it was found that both certification systems had very similar composition and rating system, however, there was slight difference in the points of emphasizing the standards due to the differences in social awareness of green buildings in each country. As a result of the detailed comparison of both standards, in addition, it was found that almost half of criteria had different evaluation approaches such as evaluation methods and scopes between both standards even though they had same evaluation objectives; some of the differences were due to the social or cultural differences between the countries, and the others were not. Through the research, we were able to derive some criteria that need for improvements of G-SEED considering the domestic conditions in Korea.

• Journal of the Institute of Electronics Engineers of Korea SC
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• v.45 no.6
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• pp.1-9
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• 2008

This paper presents transmission parameters of DH(Dog-Horse) systems based on IEEE802.16e(WiBro) systems. DH requires higher uplink multimedia data rate, which is different from commercial WiBro systems. This paper designes some transmission parameters satisfying the DH system requirements and demonstrates link performance evaluation based on the parameters. For the transmission parameters, the number dwon/up link OFDM symbols are determined by 6/30, respectively. For reliable transmission of control signals, downlink modulation and coding rate are selected by QPSK, 1/2 coding rate and that of uplink is chosen by 16QAM 1/2 and 3/4 coding rate. Based on these parameters, computer simulations demonstrate the system performance of DH system.

• KIEAE Journal
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• v.10 no.4
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• pp.19-28
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• 2010

As design tools, building performance certification systems can be applied to provide adequate guidelines on design process to create environment-friendly buildings. Domestic certification systems of residential building took effect by doing supply to designers and contractors from affiliated organization of governments in the early 2000s. As a result, Building Energy Rating System, Apartment Performance Rating System, Green Homes and other means to promote green designs have been operated. International trends of applying certification systems were started in the early 1990s as forms of LEED in USGBC, BREEAM in BRE, GBTool Canada. These systems aim to evaluate building performance in line with the Climatic Change Convention and realize sustainable building design. In 2009, residential buildings accounted for the largest portion of the internal real estate market with 67.9 percent according to the National Statistical Office data. And for 18 years since 1991, apartments among constructed residential buildings have ranked top taking up 77.7% as of 2009. Apartment performance evaluations accordingly are to promote to constitution of improving tenant quality of life, the residential environment and saving energy and resources in the internal building market. The purpose of this study is to compare and analyze valuation bases of each sector in evaluation systems of residential buildings at home and abroad to upgrade current systems through reflecting the characteristics of residential buildings. Implementation of this study basically include comparison of valuation bases and partial analysis on properties of rating systems to suggest requisites for improvement in building performance certification.

• KIEAE Journal
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• v.14 no.1
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• pp.113-120
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• 2014

The Green growth has been demanded in all industrial sectors due to environmental destruction and exhaustion of natural resources. Buildings have consumed 1/3 of the total energy and 40% of natural resources and have accounted for 50% of $CO_2$ emissions and 30-50% of waste materials. In 1991, BREEAM(Building Research Establishment Environmental Assessment Method) of the British BRE(Building Research Establishment) had begun reduction of $CO_2$ emissions and energy saving movements, in which all the countries around the world have participated. The Republic of Korea has taken part in this trend by declaring a "National Vision in the Green Growth" in 2008 and implementing regulations on "Low Carbon, Green Growth" in 2010. G-SEED(Green Standard for Energy and Environmental Design) based on GBCS(Green Building Certification System) has been actively promoted for its application. This study has limited its scope to G-SEED office buildings. It has conducted surveys of problems and assessment items of the G-SEED identified in the preceding study by the AHP(Analytic hierarchy process) method. The purpose of this study is to conduct a comparison analysis of problems and ranking of evaluation items recognized in the survey and to be presented as reference materials for G-SEED system improvements at its next amendments.

• BMB Reports
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• v.40 no.3
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• pp.358-367
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• 2007

A novel mannose-binding tuber lectin with in vitro antiproliferative activity towards human cancer cell lines and antiviral activity against HSV-II was isolated from fresh tubers of a traditional Chinese medicinal herb, Typhonium divaricatum (L.) Decne by a combined procedure involving extraction, ammonium sulfate precipitation, ion exchange chromatography on DEAE-SEPHAROSE, CM-SEPHAROSE and gel-filtration on sephacryl S-200. The apparent molecular mass of the purified Typhonium divaricatum lectin (TDL) was 48 kDa. TDL exhibits hemagglutinating activity toward rabbit erythrocytes at 0.95 $\mu$g/ml, and its activity could be strongly inhibited by mannan, ovomucoid, asialofetuin and thyroglobulin. TDL showed antiproliferative activity towards some well established human cancer cell lines, e.g. Pro-01 (56.7 $\pm$ 6.8), Bre-04 (41.5 $\pm$ 4.8), and Lu-04 (11.4 $\pm$ 0.3). The anti-HSV-II activity of TDL was elucidated by testing its HSV-II infection inhibitory activity in Vero cells with $TC_50$ and $EC_50$ of 5.176 mg/ml and 3.054 $\mu$g/ml respectively. The full-length cDNA sequence of TDL was 1145 bp and contained an 813-bp open reading frame (ORF) encoding a 271 amino acid precursor of 29-kDa. Homology analysis showed that TDL had high homology with many other mannose-binding lectins. Secondary and three-dimensional structures analyses showed that TDL is heterotetramer and similar with lectins from mannose-binding lectin superfamily, especially those from family Araceae.

• Journal of The Korean Society of Grassland and Forage Science
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• v.15 no.3
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• pp.215-221
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• 1995

Three fistulated nonlactating Holstein cows were used to examine the change in ruminal characteristics when fed diets of 100% corn silage, 50% corn silage + 50% wet brewers' grain(WBG) and 100% WBG, and also twenty Holstein heifers averaging 156 $\PM$ 3.2kg BW were randomly assingned to determine the adequate feeding level of concentrate with the following treatments ; Only WBG, concentrate to 0.5, 1.0 and 1.5% of BW under feeding WBG ad libitum. Tne main results were as follows : I. Ruminal pH was minimized 3 hrs after feeding and then gradually increased. The mean ruminal pH was highest in feeding only WBG (6.72) and lowest in only corn silage(6.0) 2. Ruminal $NH_3$-N concentration was maximized 1 hr after feeding and then gradually decreased regardless of diets, n7e average ruminal $NH_3$-N content was highest in diet of 100% WBG(12.9 rngldl) and lowest in 100% corn silage(5.9 mg/dl) 3. Acetic acid percent among VFA was highest when WBG replaced 50% corn silage and acetic to propionic acid ratio was higher in order of feeding only corn silage, 50% corn silage + 50% WBG and only WBG. 4. Ruminal bacteria and protozoa number in diet of 50% corn silage + 50% WBG were 8.14 $\times$ > $10^5$ and 1.45 $\times$ $10^5$, respectively, which were most of all treatments. 5. Daily gain and feed efficiency of Holstein heifer in feeding concentrate to 0.5% of BW and forage hay to about 20% of total DM intake when fed free WBG were better

• Journal of Embryo Transfer
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• v.25 no.4
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• pp.281-286
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• 2010

This study was to evaluate the protein profile of seminal plasma using 2-DE in Hanwoo. Seminal plasma was harvested from five mature Hanwoo, and seminal plasma protein was extracted by M-PER Mammalian Protein Extraction Reagent. Proteins were refined by clean-up kit and quantified by Bradford method until total protein was $300\;{\mu}l$. Immobilized pH gradient (IPG) strip was used 18 cm and 3~11 NL. SDS-PAGE was used 12% acrylamide gel. Each gels were visualized by comassie brilliant blue and silver staining. These spots were analyzed by MALDI-TOF MS and searched on NCBInr. The result, 20 proteins of 36 protein spots were searched through peptide sequencing on the NCBInr. 8 proteins profiled by 2-DE were proved through previous bovine studies and the name of each protein was albumin, nucleobindin, clusterin, TIMP-2, spermadhesin Z13, spermadhesin-1 and BSP proteins (BSP 30 kDa and BSP A1/A2). 12 new proteins were ATP synthase, protein MAK16 homolog, Transmembrane protein 214, E3 ubiquitin-protein ligase BRE1A, dual serine/threonine and tyrosine protein kinase, tissue factor pathway inhibitor 2, alpha-actinin-4, RUN domain-containing protein 3B, catenin alpha-1, protein-glutamine gamma-glutamyltransferase 2, plakophilin-1 and inter-alpha-trypsin inhibitor heavy chain H1 has not been previously described in the bovine seminal plasma study. These proteins may be contribute to define the type of proteins affecting fertility of male and improve the fertilizing ability of semen in Hanwoo.