• Korean Journal of Fisheries and Aquatic Sciences
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• v.48 no.6
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• pp.833-838
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• 2015

To develop a value-added product from individually quick-frozen oyster Crassostrea gigas extract (IQFOE), we prepared two types of oyster sauce (OS): bottled OS (BOS) and retort pouched OS (ROS). We investigated processing conditions, quality metrics and flavor compounds in each type of sauce. We found that the most appropriate base formular for both BOS and ROS consisted of 40.0% IQFOE (Brix $30^{\circ}$), 15.0% sugar, 6.0% salt, 4.0% monosodium glutamate, 4.0% soy sauce, 3.5% starch, 3.0% yeast extract, 3.5% wheat flour and 21.0% water. The crude protein, salinity and amino-nitrogen contents of the BOS and ROS were 8.2 and 8.3%, 9.3 and 9.2%, and 539.2 and 535.2 mg/100 g, respectively. In commercial oyster sauces (COS), these values were 4.7-6.5%, 9.7-12.0%, and 244.7-504.2 mg/100 g, respectively. The total free amino acids content of ROS was 7,346.9 mg/100 g, and the main free amino acids were glutamic acid, taurine, proline, glycine and alanine. The inosinic monophosphate (IMP) content of the ROS was 131.6 mg/100 g, and the primary inorganic ions were Na, K, S and P. The present BOS and ROS have favorable organoleptic qualities and storage stability compared with COS, and are suitable for commercialization as high-flavor seasoning sauces.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.4
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• pp.467-476
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• 2019

Objective: This research was conducted to study the genetic diversity in several Indonesian cattle breeds using microsatellite markers to classify the Indonesian cattle breeds. Methods: A total of 229 DNA samples from of 10 cattle breeds were used in this study. The polymerase chain reaction process was conducted using 12 labeled primers. The size of allele was generated using the multiplex DNA fragment analysis. The POPGEN and CERVUS programs were used to obtain the observed number of alleles, effective number of alleles, observed heterozygosity value, expected heterozygosity value, allele frequency, genetic differentiation, the global heterozygote deficit among breeds, and the heterozygote deficit within the breed, gene flow, Hardy-Weinberg equilibrium, and polymorphism information content values. The MEGA program was used to generate a dendrogram that illustrates the relationship among cattle population. Bayesian clustering assignments were analyzed using STRUCTURE program. The GENETIX program was used to perform the correspondence factorial analysis (CFA). The GENALEX program was used to perform the principal coordinates analysis (PCoA) and analysis of molecular variance. The principal component analysis (PCA) was performed using adegenet package of R program. Results: A total of 862 alleles were detected in this study. The INRA23 allele 205 is a specific allele candidate for the Sumba Ongole cattle, while the allele 219 is a specific allele candidate for Ongole Grade. This study revealed a very close genetic relationship between the Ongole Grade and Sumba Ongole cattle and between the Madura and Pasundan cattle. The results from the CFA, PCoA, and PCA analysis in this study provide scientific evidence regarding the genetic relationship between Banteng and Bali cattle. According to the genetic relationship, the Pesisir cattle were classified as Bos indicus cattle. Conclusion: All identified alleles in this study were able to classify the cattle population into three clusters i.e. Bos taurus cluster (Simmental Purebred, Simmental Crossbred, and Holstein Friesian cattle); Bos indicus cluster (Sumba Ongole, Ongole Grade, Madura, Pasundan, and Pesisir cattle); and Bos javanicus cluster (Banteng and Bali cattle).

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.8
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• pp.1069-1076
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• 2019

Objective: Yunnan is not only a frontier zone that connects China with South and Southeast Asia, but also represents an admixture zone between taurine (Bos taurus) and zebu (Bos indicus) cattle. The purpose of this study is to understand the level of genomic diversity and the extent of admixture in each Yunnan native cattle breed. Methods: All 120 individuals were genotyped using Illumina BovineHD BeadChip (777,962 single nucleotide polymorphisms [SNPs]). Quality control and genomic diversity indexes were calculated using PLINK software. The principal component analysis (PCA) was assessed using SMARTPCA program implemented in EIGENSOFT software. The ADMIXTURE software was used to reveal admixture patterns among breeds. Results: A total of 604,630 SNPs was obtained after quality control procedures. Among six breeds, the highest level of mean heterozygosity was found in Zhaotong cattle from Northeastern Yunnan, whereas the lowest level of heterozygosity was detected in Dehong humped cattle from Western Yunnan. The PCA based on a pruned dataset of 233,788 SNPs clearly separated Dehong humped cattle (supposed to be a pure zebu breed) from other five breeds. The admixture analysis further revealed two clusters (K = 2 with the lowest cross validation error), corresponding to taurine and zebu cattle lineages. All six breeds except for Dehong humped cattle showed different degrees of admixture between taurine and zebu cattle. As expected, Dehong humped cattle showed no signature of taurine cattle influence. Conclusion: Overall, considerable genomic diversity was found in six Yunnan native cattle breeds except for Dehong humped cattle from Western Yunnan. Dehong humped cattle is a pure zebu breed, while other five breeds had admixed origins with different extents of admixture between taurine and zebu cattle. Such admixture by crossbreeding between zebu and taurine cattle facilitated the spread of zebu cattle from tropical and subtropical regions to other highland regions in Yunnan.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.7
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• pp.1036-1043
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• 2019

Objective: To investigate the color and oxidative stabilities of longissimus lumborum (LL) and psoas major (PM) muscles from grain-finished Bos indicus cattle in Brazil. Methods: The LL and PM muscles were obtained 24 h post-mortem from eight (n = 8) Nellore bull carcasses, fabricated into 1.5-cm steaks, aerobically packaged, and stored at $4^{\circ}C$ for nine days. Steaks were analyzed for myoglobin concentration, pH, instrumental color, metmyoglobin reducing activity (MRA) and lipid oxidation. Results: The LL steaks exhibited greater (p<0.05) redness, color stability, and MRA than their PM counterparts on days 5 and 9. The LL and PM steaks demonstrated similar (p>0.05) lightness and yellowness on days 0, 5, and 9. On the other hand, PM steaks exhibited greater (p<0.05) myoglobin concentration, pH, and lipid oxidation than their LL counterparts. Conclusion: These results indicated that muscle source influenced the color and oxidative stabilities of beef from grain-finished Bos indicus animals. These results highlighted the necessity of muscle-specific strategies to improve the color stability of beef from grain-fed Bos indicus cattle.

• Journal of The Korean Society of Grassland and Forage Science
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• v.22 no.3
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• pp.169-176
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• 2002

To determine availability as a feed source of deer of by-products that produced at reforestation areas and comprised shrubs and wild grasses, mixed forest by-product silage(FBS), arrowroot silage(ARS), bristletooth oak tree silage(BOS), white oak tree silage(WOS) were prepared to compare dry matter intake, digestibility and nitrogen balance in digestion balance trials with spotted deer. Dry matter intake was highest in WOS and lowest in ARS but there was no significant difference(P＞0.05). Digestible dry matter intake was higher in FBS and ARS that had higher dry matter digestibility than in WOS and BOS that had lower dry matter digestibility(P＞0.05). Dry matter digestibility was highest fur ARS and lowest for BOS(P＜0.05). Digestibility of crude protein was similar to that of dry matter. That of crude fiber was higher for FBS and ARS than for BOS and WOS(P＞0.05). Daily gain of body weight was slightly greater for WOS than fer other treatments(P＞0.05). Nitrogen intake was highest for ARS and lowest for BOS(P＜0.05), and fecal nitrogen was opposite to nitrogen intake(P＜0.05). Nitrogen excretion with urine was highest for ARS that had higher nitrogen intake and lowest for BOS and there was a significant difference(P＜0.05). Retained nitrogen was highest for FBS and lowest for. BOS (P＞0.05). Although there was no significant difference(P＞0.05): the ration of nitrogen intake to retained nitrogen was highest in FBS for 43.9% and lowest in BOS for 24.7%. Consequently because by-products that produced at reforestation areas had high intake and internal availability, it was evaluated to valuable feed source for deer.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.10
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• pp.1478-1484
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• 2007

To determine the origin and genetic diversity of Red Chittagong (RC) cattle in Bangladesh, we analyzed mitochondrial DNA displacement loop (D-loop) sequences of 48 samples along with 22 previously published sequences from Bos indicus and Bos taurus breeds. Twenty five haplotypes were identified in RC cattle that were defined by 44 polymorphic sites and nucleotide diversity was $0.0055{\pm}0.0026$. The estimated sequence divergence times between RC and other zebu cattle breeds studied ranged between 22,700-26,900 years before present (YBP) which, it is suggested, predate domestication of RC cattle. Furthermore, it is assumed that introgressions have occurred in this breed mainly from Indian zebu breeds in the recent millennia. The phylogenetic studies showed RC cattle clustered with Bos indicus lineage with two distinct haplogroups representing high genetic variability of this breed. These findings can be used for designing proper breeding and conservation strategies for RC cattle in Bangladesh.

• Journal of ILASS-Korea
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• v.20 no.1
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• pp.35-42
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• 2015

This study is to investigate the spray behavior of DME-LPG blended fuels in common rail injection system for diesel engines. The visualization experiment was performed to analyze the macroscopic spray behavior of test fuels. In addition, the experiment using BOS(Background Oriented Schlieren) method is performed to compare liquid phase and gas phase. The test fuels are injected in high pressure chamber. The ambient pressure of high pressure chamber was formed by nitrogen gas. Spray tip penetration, spray cone angle and spray area were measured using high speed camera. SMD(Sauter Mean Diameter) and spray particle velocity were measured using the PDPA(Phase Doppler Particle Analyzer) system to analyze the microscopic properties of test fuels. The results of this experiment showed that spray tip penetration, spray cone angle and spray area of DME-LPG fuels are similar to those of DME fuel. When compared to results of experiment using BOS, significant differences of spray tip penetrations, spray cone angle and spray area are showed because of gas phase. The results of experiment using BOS method showed higher values. SMD of DME-LPG blended fuels is smaller than that of DME fuel. Velocity of DME-LPG blended fuels is faster than that of DME fuel.

• 한국신재생에너지학회:학술대회논문집
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• 2007.06a
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• pp.284-287
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• 2007

This paper presents the performance evaluation of PV systems installed at Tibet area of China in order to identity the key factors that determines system operation at a severe climate conditions and promote the cooperation of PV technology between Korea and China. The installed systems consist of 100kW on-grid connected PV systems, BOS(balance of systems), data acquisition and transmission equipments. The Korea side supplied the solar cell, BOS like as inverter, control box and monitoring system. And the Chinese side assembled solar module, constructed site and built control house. It has been shown that the average radiation per monthly from Tibet is 1.5 times larger than that from Mokpo. Also, radiation time from Tibet is 2hour higher than that from Korea. The economical analysis has shown that with the current prices, investment in a grid connected PV systems is generally profitable

• Journal of Korean Society of Industrial and Systems Engineering
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• v.19 no.38
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• pp.159-168
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• 1996

Appraisal format is the methods that is used to gether informations related to performance behaviors. Appraisal format has descriptive characteristics including evalustion dimensions, anchors, scales. The purpose of this paper is to study the effectiveness of a format characteristics of the appraisal results through literature review and to explore more approprate appraisal format through comparing three types of formats, graphic scales, BARS, BOS. According to the results, rating format's characteristics can affect the quality of appreisa results in that appraisal format can provide rators with evaluation standards which help raters to evaluate effectively. Specific dimensions are better than gernal one dimension. The effectiveness of scale in some degrees depends on development procedure. In general anchors are important to reduce rating errors and behavioral anchors are better than numeric anchors, The systematically developed BARS and BOS can reduce rating erros but graphic scales has limitstions.

• Journal of the Korean Society of Safety
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• v.31 no.1
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• pp.132-138
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• 2016

The purpose of this study was to develop safety competences, a set of behavioral indicators of each competence and measuring methods of behavioral indicators for preventing human error of nuclear power plants(NPPs). The safety competences and behavioral indicators were derived from the five steps consisted of derivation of preliminary competence items through literature review, content analysis, interview(FGI, BEI), examination of content validity and decision making of final indicators. The results showed that 13 core safety competences and 35 behavior indicators were derived finally. In addition, the methods of measuring safety competences or behavioral indicators such as Behaviorally Anchored Rating Scale (BARS), Behavior Observation Scale (BOS) were developed and suggested.