• Journal of Wetlands Research
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• v.11 no.2
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• pp.55-66
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• 2009

The increase of pollutant loadings from nonpoint sources affect the water quality of the major rivers in Korea. Consequently, the need for managing the nonpoint source (NPS) pollution becomes the main concern of the Korean Ministry of Environment (MOE). Recently, the policy was changed from pollutant concentration-restricting approach to the total maximum daily load (TMDL) approach to improve the water quality and protect the aquatic ecosystem. Part of the program is the construction of Best Management Practice (BMP) pilot facilities basically to control NPS. Most of the BMPs adopted were foreign technologies which could not be properly employed in the country due to some limitations such as climate, watershed characteristics, etc. In other words, to be able to apply the BMPs, research on its applicability is necessary. In this study, a three-year monitoring has been conducted to assess the treatment performance of the BMP installed in highway toll plaza and parking lot. The data gathered aid in the characterization of NPS pollutants in runoff and estimation of the pollutant removal efficiency of the BMP. The results will be used for the future implementation of BMP in different land uses as well as for the determination of optimum operation and maintenance.

• Proceedings of the Korea Contents Association Conference
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• 2009.05a
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• pp.1124-1129
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• 2009

This study aims to examine the effects of non-invasive constant microcurrent stimulation on expression of Bone Morphogenetic Protein(BMP) 4 after tibia fracture in rabbits. Twenty four rabbits with tibia fracture were randomly divided into control and experimental groups. Each group was divided into four subgroups, based on the duration of the experiment (3, 7, 14, 28 days). The experimental groups received a constant microcurrent stimulation of $20{\sim}25{\mu}A$ intensity with surface Ag-AgCl electrode (diameter 1cm, Biopac, U.S.A.) for 24 hours a day. Cathode of the microcurrent stimulator located on the tibia directly, anode of it did on the gastrocnemius muscle. Rabbits were sacrificed on each of the postoperative days 3, 7, 14, 28. To investigate how non- invasive constant microcurrent stimulation affects bone healing, immunohistochemical analysis of BMP-4 was performed at each point. After evaluation, the test results are as follows: Comparisons of immunohistochemical observation of BMP-4 in 7 days after tibial fracture show that there was shown to be a moderate positive reaction (++) on concentric circles of Harversian system andt he interstitial lamella in the control group, while there was a very strong positive reaction (++++) on concentric circles of Harversian system and interstitial lamellain the experimental group. These results suggest that applying non-invasive constant microcurrent stimulation on fractured bone is helpful to bone healing.

• Journal of the Institute of Electronics and Information Engineers
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• v.50 no.12
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• pp.91-97
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• 2013

Image and it's processing techniques are widely applied and very important in the recent IT environment. In this paper, we try to reconstruct original BMP(Bitmap) image into indexed image and codebook utilizing vector quantization and represent high quality image only with same pixel depth of previous indexed image like JPEG etc. That is, By adopting common map method onto index image with $2^n$ color codebook, image can be represented as high quality as $2^{n+1}$ color codebook. When proposed output image is compared with original BMP image, it provides as much around 2dB as higher PSNR than conventional 8 bit index image(normal JPEG). Furthermore, this improvement(2 dB higher PSNR) could be provided when using the 9 bit indexed image.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.23 no.3
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• pp.258-262
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• 2001

Distraction osteogenesis(DO) can be performed in the bony defect associated with trauma, anomaly, and various kinds of disease. The gap generated by DO is filled with growing callus : during the period of distraction, the osteogenesis is continued. However, there have been few reports about expression pattern of growth factors in newly formed bone during the consolidation periods. We performed DO in the mandibular defect case and studied the expressed pattern of growth factors. Its pattern was compared to that of the same patient. BMP-2 and -4 were strongly expressed in the DO site. Particularly, BMP-4 was not expressed in the normal mature bone, but expressed in new bone in DO. However, there was no difference in the FGF-7 expression between the sites. Therefore, strong expression of BMP-4 are related to new bone formation in DO and they may not be related to the normal homeostasis in human bone. Though FGF-7 is related to the growth of keratinocyte, it may have minimal role in the DO and normal mature bone.

• Journal of Life Science
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• v.10 no.2
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• pp.218-227
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• 2000

Bone morphogenetic protein 1 (BMP-1) is part of a complex capable of inducing ectopic bone formation in mammals. Studies on TGF-β1 processing and Drosophila dorsal-ventral patterning have focused attention on BMP-1 as important in mediating the biological activity of this bone inducing complex. Herein, the bacterial expression, refolding, purification, and initial characterization of the BMP-1 proteolytic domain (BPD) are described. A semi-quantitative fluorescence-based thin layer chromatography assay was developed to assist in rapidly screening for optimal renaturation conditions. According to a preliminary screen for optimal conditions for the refolding of BPD , a detectable proteolytic activity against a high turnover substrate for astacin, a homologous protease from crayfish was observed. The conditions identified have allowed the expression of sufficient amounts of BPD for the characterization of the protein. Its proteolytic activity exhibits the same cleavage specificity as astacin against seven substrates that were previously synthesized for studying astacin. Furthermore, this activity is inhibited by the metal chelator 1,10-phenanthroline but not by its analogue 1,7-phenanthroline. The collagenase inhibitor Pro-Leu-Gly hydroxamate was found to inhibit both astacin and BPD activity. The results presented in this paper argue that BMP-1 does in fact possess an intrinsic proteolytic activity.

• Journal of the Korean GEO-environmental Society
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• v.15 no.9
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• pp.13-18
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• 2014

BMP test was carried out to evaluate the characteristics for co-digestion of night soil and food waste. 6 types of sludge were tested in 30 days which were raw, excess, digested, night soil/septic tank (1:1), food waste (food : dilution water = 1:1), and mixed sludge. Bio gas was produced actively after 2 days, and continued in 2 weeks. Gas generation amount was decreased rapidly after considerable space of time. Especially maximum productivity of gas was shown in 7~8 days. The ultimate methane yields of raw, excess, digested, night soil/septic tank, food waste, and mixed sludge were 64.63, 67.49, 66.45, 72.44, 107.85, and 46.71 mL $CH_4/g$ VS respectively from Modified Gompertz model. The lag growth phase time and maximum specific methane production rate of mixed sludge were 1.88 day and 80.4 mL/day respectively. The methane potential of mixed sludge was higher than individual sludge. So high methane potential was expected by controlling mixing ratio of food waste. Besides stable operation of digestion tank and the solution of oligotrophic problem were possible.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.40
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• pp.34.1-34.8
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• 2018

Background: Radiation therapy is widely employed in the treatment of head and neck cancer. Adverse effects of therapeutic irradiation include delayed bone healing after dental extraction or impaired bone regeneration at the irradiated bony defect. Development of a reliable experimental model may be beneficial to study tissue regeneration in the irradiated field. The current study aimed to develop a relevant animal model of post-radiation cranial bone defect. Methods: A lead shielding block was designed for selective external irradiation of the mouse calvaria. Critical-size calvarial defect was created 2 weeks after the irradiation. The defect was filled with a collagen scaffold, with or without incorporation of bone morphogenetic protein 2 (BMP-2) (1 ㎍/ml). The non-irradiated mice treated with or without BMP-2-included scaffold served as control. Four weeks after the surgery, the specimens were harvested and the degree of bone formation was evaluated by histological and radiographical examinations. Results: BMP-2-treated scaffold yielded significant bone regeneration in the mice calvarial defects. However, a single fraction of external irradiation was observed to eliminate the bone regeneration capacity of the BMP-2-incorporated scaffold without influencing the survival of the animals. Conclusion: The current study established an efficient model for post-radiation cranial bone regeneration and can be applied for evaluating the robust bone formation system using various chemokines or agents in unfavorable, demanding radiation-related bone defect models.

• Proceedings of the Korea Water Resources Association Conference
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• 2012.05a
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• pp.781-781
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• 2012

본 연구에서는 수문-수질 모의가 가능한 SWAT (Soil and Water Assessment Tool) 모형을 이용하여 경안천 상류의 별미천 유역($1.21km^2$)을 대상으로 최적관리기법(Best Management Practice, BMP) 적용에 따른 비점오염원 저감효과를 분석하고자 한다. 이를 위해, 별미천 유역내 논($0.049km^2$)과 밭($0.018km^2$) 지역에 대한 BMP 시나리오를 작성하였다. 먼저, 논에서는 계단식(Terrace) 재배 방법을 적용하였으며, 밭에서는 등고선(Contour farming) 재배 방법과 볏짚지표피복 시나리오를 적용하였다. 여기서, 볏짚지표피복 시나리오는 현재 $1276.6m^2$, 경사도 약 3.2%의 콩 재배가 이루어지고 있는 시험포장에서 수행된 결과를 이용하여 SWAT 모형의 지표유출관련 매개 변수를 조정하였다. SWAT 모형의 적용성 평가를 위해, 1:5,000 수치지도로부터 2m DEM(Digital Elevation Model) 및 QuickBird(2006.05.01) 위성영상으로부터 스크린 디지타이징기법(On-Screen Digitizing Method)을 이용하여 총 21개 토지이용항목의 1:5,000급 정밀토지이용도를 작성하고, 농촌진흥청에서 제공하는 1:25,000 정밀토양도를 구축하였다. 또한, 유역출구점에서 자동수위 및 강우량 계측기, 수질측정을 위한 오토샘플러를 설치하여 시간당 수위(유량), 강우 자료와 강우발생에 따른 수질자료(Sediment, Total Nitrogen, Total Phosphorus)를 이용하여 모형의 검보정(2011.06.08 ~ 2011.10.31)을 실시하였다. 모형의 검보정 후, 논에서의 Terrace 및 밭에서의 Contour farming, 밭 시험포장에서의 저감효과가 분석된 지표피복시나리오의 적용을 위한 SWAT 모형의 관련 매개변수를 정의하고, 각 BMP 시나리오별 비점오염원 저감효과를 분석하였다.

• Proceedings of the Korea Water Resources Association Conference
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• 2020.06a
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• pp.200-200
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• 2020

본 연구에서는 SWAT 모형과 APEX-Paddy 모형의 연계 모델링을 통한 대표 BMP(Best management practice) 적용, 정식시기 및 벼 생육기간을 고려한 시나리오 적용을 통해 농업용수의 관리 및 수질환경 개선 등에 활용할 수 있는 저영향 영농활동을 분석하고자 하였다. 만경강 유역을 대상으로 SWAT 모형을 구축하고 유역 내에 위치한 논 시험포장을 대상으로 강우-유출 및 비점오염원 모니터링 자료를 활용하여 APEX-Paddy 모형을 구축하였다. SWAT 모형과 APEX 모형을 연계하여 유역의 수문, 수질에 대한 정밀한 모델링을 수행하였으며, 이는 저영향 영농활동을 분석하기 위한 필드단위의 정확한 결과를 유역차원에 반영하기 위함이다. 특히, 본 연구에 사용된 APEX-Paddy 모형은 농촌진흥청과 Texas A&M의 공동연구를 통해 개발된 새로운 모형으로서 한국의 논 영농활동 및 담수환경을 반영하여 논에서의 유출 및 비점오염원을 모의할 수 있다. 연계 모형의 적합성 평가를 위해 R² (Determine of Coefficient), RMSE (Root mean square error), NSE (Nash-sutcliffe efficiency)를 사용하였다. 적합성 평가 지표를 분석한 결과, 유출량은 R² 평균 0.91, RMSE 평균 2.87 mm/day, NSE 평균 0.78로 나타났다. T-N 부하량은 R² 평균 0.74, RMSE 평균 59.3 kg/ha/day, NSE 평균 0.50으로 나타났다. 저영향 영농활동 관리방안을 위한 시나리오로 1) 논의 물꼬높이(BMP) 관리 적용, 2) 벼 생육기간 조절을 고려하여 기온변화에 따른 정식시기, 벼 생육기간 등을 조정하여 적용하였다. 기후변화 시나리오는 10개 GCM 모델의 RCP 8.5 시나리오를 통해 분석하였으며, 유역차원의 미래 영향을 분석한 결과, 물꼬관리 BMP에 따라 담수심이 증가되며, 관개량이 감소하고 유출량 10.7%, T-N 11.2% 저감되는 것을 나타냈으며, 벼 생육기간 조절은 BMP보다 상대적으로 효과가 높진 않았지만, 유출량 1.4%, T-N 3.1%의 저감효과를 나타냈다. 따라서 두 가지의 저영향 영농활동 관리방안은 미래기간의 기후변화에 대응하여 농업용수 및 물관리에 도움이 될 것으로 사료된다. 하지만 본 연구결과는 모델링 결과에 의존한 것이며, 추후 지속적인 연구와 보완이 필요하다.

• Journal of Veterinary Clinics
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• v.39 no.2
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• pp.59-64
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• 2022

A dog aged two years and seven months and a cat aged seven years were referred owing to fractures of long bones. Preoperative radiographs revealed comminuted bone fractures close to joints. Conventionally, long-bone fractures are treated using intramedullary pins, plate and screw systems, or an external fixator system. In cases of non-reducible fractures, various graft materials have been used in fracture treatments to stimulate bone repair. Here, recombinant human bone morphogenetic protein-2 (rhBMP-2) and a collagen membrane were applied. Four weeks after surgery, fractured bone fragments began to unite and the bone union was observed using radiography four months after surgery. No complications occurred related to grafted materials. We successfully applied rhBMP-2 and collagen membranes in two different species to support the healing process of comminuted fractures, according to the concept of guided bone regeneration.