• International Journal of Industrial Entomology and Biomaterials
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• 제4권1호
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• pp.51-56
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• 2002

A recombinant transfer vector pBacSCF was constructed by inserting huamn stem cell factor (hSCF) cDNA into plasmid pBacPAK8. BmN cells were co-transfected with modified Bombyx mori, nuclear polyhedrosis virus (BmBacPAK) DNA and the recmbinant transfer vector to construct a recombinant baculovirus containing hSCE gene. DNA dot blotting and RNA dot blotting demonstrated that the hSCE gene was contained in the recombinant virus and transcribed. The recombinant baculovirus was infectious to BmN cells and to silkworm. SDS-PAGE analysis showed a specific band of expressed product in the extract of infected cells and in the heamolymph of infected larvae. Bioactivity of the recombinant hSCE was determined with W-1 cell line and MTT colorimetric method in synergy with interlukin-3 (IL-3). These results revealed that the hSCF gene was over-expressed in cultured cells and lavae of silkworm.

• 전자공학회논문지
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• 제50권1호
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• pp.117-123
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• 2013

본 논문은 symmetric balance incomplete block design(BIBD) code의 BER=$10^{-9}$을 만족하는 spectral efficiency를 구하였다. 이 계산 결과 effective power가 큰 경우 ($P_{sr}=-10$ dBm)는 m=2로 고정시키고 q값을 변화시키는 ideal BIBD code구성이 효율적이었다. 이와 반대로 effective power가 작은 경우 ($P_{sr}=-25$ dBm)는 ideal BIBD code 구성 보다는 q > 2인 값을 취하고 m값을 변화시키는 설계가 더 효율적임을 알 수 있었다.

• 전자공학회논문지A
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• 제30A권5호
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• pp.45-51
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• 1993

An erbium doped fiber amplifier(EDFA) pumped by aingle 1.48$\mu$m LD was fabricated, and its gain and noise characteristics were measured. As a signal source, 1548 nm wavelength DFB LD was used. The small signal net gain of the EDFA module was 21.8 dB with maximum gain coefficient 0.7dB/mW for the erbium fiber length of 17.6 m, the pump power of 58 mW, and an input signal power of -25 dBm, respectively. The saturation power of the EDFA was 1 dBm for the input signal power of -5 dBm and the noise figure, measured by using an optical spectrum analyzer, was 5.8 dB for the input signal power of -40 dBm.

• International Journal of Industrial Entomology and Biomaterials
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• 제17권1호
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• pp.121-124
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• 2008

The caspase family proteins play an important role in programmed cell death (apoptosis). To date, the expression profiles of the caspase family genes in Bombyx mori (Bm) are poorly known. In this study, we examined the expression profiles of two novel Bm caspase family genes (ice-2 and ice-5), the potential change of the mitochondrial membrane and the morphology in Bm cells after stimulation of ultraviolet (UV) irradiation. The results showed the potential change of the mitochondrial membrane occurred at 5 hours after UV irradiation treatment. Analysis of fluorescent quantitative RT-PCR demonstrated that both the ice-2 and ice-5 might be involved in UV induced apoptosis in Bm cells. Notably, after UV irradiating, expression pattern of ice-2 and ice-5 were remarkably different. The ice-2 gene was highly expressed at two time points, 0.5 and 5 hours after UV stimulating, while the expression level of ice-5 only peaked at 5 hours after UV stimulating. It indicated that apoptosis induced by UV irradiation was involved in the mitochondrial pathway and the two isoforms of Bm ice may act but play different role during the apoptosis of Bm cells.

• Asian-Australasian Journal of Animal Sciences
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• 제19권5호
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• pp.739-743
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• 2006

Postmortem proteolysis of breast (BM) and leg (LM) muscles from Taiwan colored chickens (TCC) and silkie bantams (SB) at $5^{\circ}C$ were compared. Myofibrils were prepared from BM and LM samples that were randomly taken from the carcasses of SB and TCC after 0, 1, 3, 7 and 14 days of storage at $5^{\circ}C$. pH of samples was determined, and degradation of myofibrillar proteins was analyzed by the SDS-PAGE and western blots. The results showed that pH was lower in BM than in LM samples from both avian strains. Appearance of 30 kDa components and disappearance of titin and nebulin were more rapidly as seen on SDS-PAGE in BM than in LM samples. Western blots labeled with a monoclonal antibody to desmin also demonstrated that desmin degraded more quickly in BM samples. Our data might suggest that postmortem proteolysis occurred more rapidly in BM than in LM from both TCC and SB.

• 전자공학회논문지
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• 제54권5호
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• pp.123-127
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• 2017

본 논문에서는 FET의 채널저항을 이용한 이미지 제거 혼합기를 제작하여 그 특성을 분석하였다. 이 혼합기는 IF 50MHz~90MHz, LO 8.17GHz 및 RF 8.08GHz~8.12GHz로써 8GHz대역의 64QAM에 적용할 수 있도록 하였다. 측정 결과 -20dBm의 IF 신호와 10dBm의 LO 신호를 인가하였을 때 -33.2dBm의 RF 출력을 얻었으며, 약 12.9dB의 변환손실을 보였으며, 8.1GHz RF 신호에 대하여 LO 신호는 14.3dB, 이미지 신호는 10.4dB의 억압특성을 얻을 수 있었다. 또한 2-tone실험 결과 51.7dBc의 IMD 특성을 얻을 수 있었다.

• International Journal of Industrial Entomology and Biomaterials
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• 제29권2호
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• pp.145-152
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• 2014

The variation in the level of immune response related gene expression in silkworm, Bombyx mori following infection with Bombyx mori nucleopolyhedrovirus (BmNPV) was analyzed at different time intervals. The occlusion bodies of BmNPV orally inoculated to the two most divergent silkworm races viz., Sarupat (resistant to BmNPV infection) and CSR2 (susceptible to BmNPV infection) were subjected to oral BmNPV inoculation. The expression profile of gp 41 gene of BmNPV in the Sarupat and CSR2 races revealed that the virus could invade the midguts of both susceptible and resistant races. However, its multiplication was significantly less in the midgut of resistant race, while, in the susceptible race, the viral multiplication reached maximum level within 12 h. These findings indicate that potential host genes are involved in the inhibition of viral multiplication within larval midgut. The immune response genes arylphorin, cathepsin B, gloverin, lebocin, serpin, Hsp 19.9, Hsp 20.1, Hsp 20.4, Hsp 20.8, Hsp 21.4, Hsp 23.7, Hsp 40, Hsp 70, Hsp90 revealed differential level of expression on NPV infection. The gloverin, serpin, Hsp 23.7 and Hsp 40 genes are significantly up-regulated in the resistant race after NPV infection. The early up-regulation of these genes suggests that these genes could play an important role in baculovirus resistance in the silkworm, B. mori.

• 한국통신학회논문지
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• 제25권7A호
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• pp.935-942
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• 2000

본 논문에서는 1.6GHz PCS 대역 초소형 전압제어발진기의 설계개발하였다. 다층 PCB 구조를 갖는 Colpitts 형의 LC 동조형 발진기를 설계하교 회로 시뮬레이터를 이용하여 회로특성을 최적화하였다. 최적화된 설계 데이터를 이용하여 6$\times$6$\times$1.8 ㎣(0.065cc) 크기의 소형 VCO를 제작한 후 시험하였다. 개발된 VCO는 52.3MHz 튜닝범위에서 -1.67dBm $\pm$0.5dBm 의 일정한 출력레벨을 갖었으며 10kHz offset 주파수에서 -99.33dBc/Hz 의 우수한 위상잡음 특성을 나타내었다.

• 한국잠사곤충학회지
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• 제38권1호
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• pp.42-47
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• 1996

AcNPV와 BmNPV를 배양세포주에서 동시감염시켜 선발한 숙주범위가 넓어진 재조합 바이러스인 RecB-727과 RecS-A6의 분자생물학적인 특성들을 조사하였다. 재조합 바이러스의 LT50 값을 조사한 결과, RecS-A6는 모바이러스인 BmNPV 보다 비교적 낮은 병원성을 보았으나 RecB-727은 거의 비슷한 수준의 높은 병원성을 나타내었다. 재조합 바이러스 DNA를 분리하여 모바이러스 DNA와 함께 제한효소 패턴을 비교한 결과 DNA 수준에서 재조합이 일어났음을 확인할 수 있었으며, 일부 유전자의 재조합을 예측할 수 있었다. 또한 p10 유전자에 대한 Southern blot 분석 결과 RecB-727의 p10 유전자는 AcNPV에서 유래되었으며, RecS-A6는 BmNPV의 p10 유전자를 갖고 있는 것으로 추정된다. 재조합 바이러스의 숙주범위 확장에 중요한 역할을 하는 것으로 알려진 DNA helicase 유전자 내의 HindIII-SacI 0.6kb 부위에 대하여 약 250 bp의 염기서열을 조사한 결과, 이 부위의 염기서열은 BmNPV helicase의 염기서열과 동일하였다.

• 한국전자파학회논문지
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• 제17권2호
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• pp.198-206
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• 2006

데이터 통신용 400 MHz ISM 대역 송${\cdot}$수신기를 설계하였다. 전파 법규의 8.5 kHz 이하 점유 주파수 대역폭을 만족하기 위해 GFSK 변조 방식을 이용하였고, 8051 CPU에 통신 프로토콜을 이식하여 데이터를 송${\cdot}$수신하였다. 제작된 송${\cdot}$수신기는 ${\pm}3\;kHz$이하의 주파수 변조도와 20 dB의 SINAD에서 -107 dBm의 감도를 나타내었으며, 인접 채널 평균 전력을 -60 dBc 이하로 전파 법규를 만족하였다. 제작된 송수신기의 BER 특성은 입력 신호 전력이 -110 dBm에서 $1.8{\times}10^{-3}$, -106 dBm 이상일 경우 0으로 측정되었다.